22 results about "Organogenesis" patented technology

The application discloses a method for high-frequency regeneration of Rhododendron chrysanthum based on cotyledon node indirect organogenesis, and belongs to the field of plant biotechnology. The method takes the cotyledon node of Rhododendron chrysanthum sterile seedling as an explant, and obtains a complete regenerated plant through the induction of callus, differentiation of adventitious buds, elongation of adventitious buds, strong seedling and rooting culture in sequence. The application realizes the super-high-frequency differentiation of adventitious buds by combining the cotyledon node explant with a specific hormone combination for the first time, the differentiation coefficient is as high as 96, and the rooting rate is 92.7%, thereby effectively solving the technical bottleneck of low propagation efficiency of Rhododendron chrysanthum, and providing reliable technical support for the conservation and industrialization development of Rhododendron chrysanthum germplasm resources.

The invention provides a corydalis pygmaea terminal bud organogenesis and in-vitro rapid propagation method, and belongs to the technical field of plant tissue culture. Comprising the following steps: by taking a terminal bud of a corydalis pygmaea aseptic seedling as an explant, sequentially performing adventitious bud differentiation culture, cluster bud multiplication culture and rooting induction to obtain a corydalis pygmaea tissue culture seedling, pretreating a root system of the corydalis pygmaea tissue culture seedling with nano-zinc oxide, and transplanting the corydalis pygmaea tissue culture seedling. According to the method, the terminal bud is used as the explant, the organ occurrence rate and the propagation coefficient are remarkably increased by optimizing the culture medium formula and the culture conditions, the induction rate reaches 85% or above, the browning rate is controlled to be 10% or below, the proliferation multiple per four weeks can reach 11-14 times, the rooting rate exceeds 90%, rapid and efficient propagation of corydalis pygmaea is achieved, reliable technical support is provided for large-scale production, and the method has a wide application prospect. The problem of raw material supply can be effectively solved, and a foundation is laid for industrial production of active ingredients.

The invention discloses a method for high-frequency regeneration of rhododendron micranthum based on cotyledonary node indirect organogenesis, and belongs to the technical field of plant biology. According to the method, a cotyledonary node of a rhododendron micranthum aseptic seedling is used as an explant, and a complete regenerated plant is obtained through callus induction, adventitious bud differentiation, adventitious bud elongation, seedling strengthening and rooting culture in sequence. According to the method, the cotyledonary node explant is combined with the specific hormone combination for the first time, the ultra-high frequency differentiation of the adventitious buds is realized, the differentiation coefficient is as high as 96, the rooting rate reaches 92.7%, the technical bottleneck of low propagation efficiency of rhododendron micranthum is effectively solved, and reliable technical support is provided for germplasm resource preservation and industrial development of rhododendron micranthum.

The present application relates to the technical field of plant induction regeneration, and particularly relates to a method for improving artificial propagation efficiency of Trichosanthes cucumerina var. villosa, which uses Trichosanthes cucumerina var. villosa stem segments as explants and optimizes the proliferation medium, fixes the excellent traits of the female parent to the greatest extent through direct organogenesis, avoids the variation possibly caused by indirect organogenesis, and has a proliferation cycle of 40 days and a propagation coefficient of more than 14.0; the method meets the needs of large-scale production of high-quality Trichosanthes cucumerina var. villosa seedlings, solves the bud tip necrosis phenomenon commonly existing in in vitro rapid propagation of cucurbitaceae plants, improves the quality of seedlings, and improves the economic benefits; the present application has important significance and value for artificial rapid propagation of Trichosanthes cucumerina var. villosa, and lays a technical foundation for protecting the wild resources and developing artificial planting.

The disclosed subject matter provides techniques for culturing organoids or cells. A device for culturing organoids can include an access port configured to receive a solution, a loading chamber, wherein the access port is located in the loading chamber, and a plurality of culture chambers, wherein the culture chambers are radiated from the loading chamber so that the solution injected into the loading chamber through the access port is distributed into the plurality of culture chambers, wherein the plurality of culture chambers are open to an external environment and comprises a protruding edge at an opening of the plurality of culture chambers.

The present application belongs to the technical field of plant induced regeneration, and particularly relates to a high-efficiency artificial propagation method of Dendrobium candidum, comprising the following steps: taking two-year-old stem segments with nodes as materials, cutting the stem segments into appropriate sizes, and disinfecting the stem segments; and culturing the stem segments in a culture medium A for 90 days, and then completing the proliferation and rooting integration through a direct organogenesis route. The present application realizes the cyclic production of Dendrobium candidum, and the whole culture process can be completed only by using one culture medium, greatly improving the artificial in-vitro propagation efficiency, and the proliferation coefficient is completely comparable to the seed asymbiotic germination method, and the problems of large offspring trait separation and different qualities caused by the seed asymbiotic germination are solved, and the quality and yield of seedlings are greatly improved. The present application can provide a brand-new technical support for the factory-scale growth of Dendrobium candidum, so as to achieve the purpose of cultivating seedlings with genetic stability and excellent quality to improve the artificial planting efficiency, and the cost is low, the cycle is short, and the quality and survival rate of seedlings are high.

The present disclosure relates to a modified plant SYMRK polypeptide that constitutively induces symbiotic organogenesis at a higher level than an unmodified plant SYMRK polypeptide under the same conditions, or induces symbiotic organogenesis in the absence of rhizobium and / or arbuscular mycorrhizal fungi recognized by a plant, and its use in a plant.

The application discloses a direct organogenesis genetic transformation method based on spinach stem tips and axillary buds, and relates to the technical field of plant genetic transformation. pCAMBIA1305-GUS As a plant expression vector, the Agrobacterium GV3101 (pSoup-p19) is used as a receptor Agrobacterium to establish a stable genetic transformation system of spinach. The application utilizes the regeneration capacity of the spinach stem tip meristem and axillary bud meristem, can improve the regeneration efficiency and transformation efficiency, and significantly shortens the genetic transformation cycle. The system can be used for the function research of key regulation genes of agronomic traits of spinach and the cultivation of excellent spinach varieties.

Chrono-Evolution BioMorph Visor Pod with Layered Organogenesis Projection Chamber

Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.

The invention discloses a Taiwania flousiana tissue culture and rapid propagation method, and relates to the technical field of nursery stock cultivation, the method comprises an explant wound pretreatment stage, a light regulation organ generation culture stage and an ex vitro rooting mycorrhizal transplanting stage, and the explant wound pretreatment stage adopts a sandwich grafting method to insert a young stem tip tissue between a scion and a stock. According to the sandwich grafting method, the young stem tip tissue is inserted between the scion and the stock, and the mature effect of the explant of the adult superior tree is effectively broken through physiological age resetting of a triple structure. By means of the method, the activity of polyphenol oxidase of the explant is greatly reduced, the germination rate is greatly increased, the excellent hereditary character of a female parent is kept for new branches, and the contradiction between regeneration capacity decline of adult materials and hereditary uncertainty of young materials is solved. Directional regulation and control of organ generation are achieved through staged application of the adjustable LED illumination module, and the whole cultivation period is compressed. Stable mass production is achieved through a standardized operation process, and the requirements of large-scale afforestation for high-quality nursery stocks are met.

Disclosures herein are directed to populations of cells comprising one or more cell of a first mammalian species and one or more cell of a second mammalian species, such as stem cells and embryos, wherein the cells are engineered to express an either an antigen-binding protein or an antigen, wherein each are expressed with a cell membrane signaling peptide and / or a cell membrane anchor protein. The inclusion of the cell membrane signaling peptide or a cell membrane anchor protein enhances cell adhesion, enabling improved formation of interspecies chimeras, for example the generation of a human and non-human interspecies chimera. Also provided herein are methods of manufacturing the population of cells as described herein. Also provided are methods of organogenesis to generate an organ, organoid, or tissue mass comprising an interspecies chimeric animal for organ transplant, and methods of treatment using the same.

The application discloses a high-flux propagation method of Panacis ginseng C.A.Meyer seedlings mediated by a direct organogenesis pathway, and comprises the following steps: after the surface of a current-year tender stem section of a fine strain of Panacis ginseng C.A.Meyer is disinfected, the stem section is cut into sections, and the sections are inoculated into an axillary bud germination induction culture medium to be cultured; leaf blades obtained in the culture are used as explant materials, the explant materials are cut into sections, and the sections are inoculated into an adventitious bud induction culture medium to be cultured for induction of the adventitious buds; the sections are transferred into an adventitious bud proliferation and elongation culture medium to be cultured for proliferation and elongation of the adventitious buds; the obtained elongated buds are separated from the bud clusters, immersed into a solution added with K-IBA and IAA to be treated, and then transferred into an adventitious root induction culture medium to be cultured for induction of the adventitious roots; after domestication, washing and surface disinfection, the seedlings are transplanted to be cultured. The application provides a rapid, efficient and high-quality Panacis ginseng C.A.Meyer tissue culture propagation method which can keep the fine characteristics of the mother plant, and provides certain technical support for germplasm preservation, large-scale propagation and variety improvement of the fine strain of Panacis ginseng C.A.Meyer.

The invention discloses a wolfberry tissue culture and rapid propagation method based on multiple organogenesis, and belongs to the technical field of plant rapid propagation. The method comprises the following steps: preparing in-vitro tissues from Ningxia wolfberry tissue culture seedlings, putting the in-vitro tissues into an adventitious bud induction culture medium, and culturing to obtain adventitious buds; the adventitious buds are placed in an adventitious bud elongation culture medium to be cultured for one month, and elongated adventitious buds are obtained; and cutting off the elongated adventitious buds, placing the adventitious buds in a rooting culture medium, and culturing to obtain a complete Ningxia wolfberry plant. According to the method, the adventitious bud induction rate of in-vitro leaves on a culture medium added with 0.5 mg / L of 6-BA and 0.5 mg / L of NAA is the highest and is 48.00%; the adventitious bud induction rate of the in-vitro stem segment on a culture medium added with 0.5 mg / L of 6-BA and 0.1 mg / L of NAA is the highest and is 58.82%; the adventitious bud induction rates of the in-vitro roots on a culture medium added with 0.05-0.5 mg / L of 6-BA and 0.05 mg / L of NAA are all 100%.

The application discloses application of a soybean GmSTM17 gene in regulation of soybean cotyledon node organogenesis and belongs to the technical field of biological breeding. In order to improve the regeneration potential in the process of soybean cotyledon node organogenesis, the application provides a method for regulating soybean cotyledon node organogenesis, which comprises regulating expression of a GmSTM17 protein coding gene in a receptor soybean to regulate cotyledon node organogenesis of the receptor soybean, and the amino acid sequence of the GmSTM17 protein is sequence 3. The application also provides application of the GmSTM17 protein in regulation of the regeneration capacity of plant tissues. It is disclosed that the GmSTM17 gene has an inhibiting effect on the growth and development of the clump-bud meristem cells, thereby negatively feeding back regulating the regeneration potential, regeneration efficiency and regeneration rate of the soybean cotyledon node explants. The application provides a theoretical basis for further analyzing the soybean regeneration mechanism and cultivating a soybean variety with high regeneration capacity.

Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.

Stretchable electronics implanted during tissue formation allow chronic studies of tissue-wide electrophysiology. This disclosure describes integrated, stretchable mesh nanoelectronics implanted during organogenesis of human stem cell-derived pancreatic islets, allowing for the long-term, stable recording of single-cell extracellular spike bursting dynamics. By employing a conventional spike sorting pipeline, two major electrical states for both α and β cells were identified based on their responses to glucose threshold for action potential firing. Continuous recording during further maturation by entrainment to 24-hour feeding cycles showed islet-level hormone secretion rhythms reflect increased and sustained coordination between α and β stimulated electrical activities, linked to cell-cell communication and exocytic network induction, revealing a role for circadian rhythms in coordinating system- level stimulus-coupled responses. These results highlight the ability for "cyborg" islets to advance efforts to generate fully functional in vitro islets for research and therapeutic applications.

The invention provides an LdBBM13 gene for regulating organogenesis and somatic embryogenesis of Lanzhou lily and application of the LdBBM13 gene, and belongs to the field of research on growth and development of bulb flower organs. The functions of the LdBBM13 gene in organogenesis and somatic embryogenesis are disclosed by adopting a VIGS silencing technology and a heterologous overexpression method and combining histomorphological observation and transcriptional regulation analysis. Researches show that overexpression of the LdBBM13 gene can significantly improve the induction rate of arabidopsis thaliana calluses, and silencing of the gene can inhibit generation of Lanzhou lily scale induced somatic embryos. The LdBBM13 gene provides a basis for developing a transgenic lily bulb marker gene related to organogenesis by using the LdBBM13 gene, and the Lanzhou lily bulb regeneration and seedling breeding process can be accelerated.

The present invention describes a microstructured device for the implementation, observation and characterization of organoids / spheroids as three-dimensional cell models suspended in time-lapse and / or confocal microscopy with immersion objectives, wherein these cell models are suitable for the study of organogenesis, carcinogenesis and drug screening. The invention is also related to the method of production of said device.

The invention discloses a method for inducing and regenerating calluses of a noniploid bamboo reed panicle, which comprises the following steps: S1, carrying out induction culture on the disinfected noniploid bamboo reed panicle in a callus induction culture medium to obtain the calluses; s2, carrying out multiplication culture on the callus in a callus multiplication culture medium to obtain a callus group; s3, performing differentiation culture on the callus clusters in a differentiation culture medium, and after the callus clusters grow into plantlets, performing seedling multiplication culture in a seedling multiplication culture medium to obtain tissue culture seedlings; s4, performing rooting culture on the tissue culture seedlings in a rooting culture medium to obtain nine-ploid bamboo reed rooting seedlings; s5, domesticating the nine-ploid bamboo reed rooting seedlings to obtain regenerated nine-ploid bamboo reed domesticated seedlings, and then planting the regenerated nine-ploid bamboo reed domesticated seedlings; according to the method, the nineploid bamboo reed spica is used as an explant, and the steps of induction culture, multiplication culture, differentiation culture, seedling multiplication and rooting culture are sequentially performed through an organ generation way, so that multiplication and differentiation into a complete plant by utilizing induced calluses are realized.

The invention belongs to the field of plant biotechnology breeding, and particularly relates to an organogenesis approach-based alfalfa rapid tissue culture regeneration method, which comprises the following steps: disinfecting and sterilizing alfalfa seeds, placing the seeds in a seed culture medium for dark culture and vernalization, and alternately culturing the vernalized seeds in a light environment and a dark environment, enabling the seeds to germinate cotyledons; cutting off cotyledons, transferring the cotyledons into a bud induction culture medium, alternately culturing in a light environment and a dark environment, and inducing to obtain cluster buds; transferring the cluster buds into a bud elongation culture medium, and alternately culturing in a light environment and a dark environment to enable the cluster buds to grow, so as to obtain highly developed cluster buds; and transferring the highly developed cluster buds into a rooting culture medium, alternately culturing in a light environment and a dark environment, and culturing and rooting to obtain a complete plant. According to the regeneration material obtained by the method, the conventional tissue culture period of 6-8 months can be shortened to only 1-2 months, and the tissue culture regeneration time is greatly shortened.