133 results about "Cambial layer" patented technology

The invention discloses a stem cell tissue culture seedling raising technique. The technique comprises the steps of 1, taking a fresh plant, conducting sterilization on the surface and cutting the plant; 2, conducting tissue differentiation and reserving a cambium; 3, culturing the cambium on an isolation medium. According to the technique, existing methods are improved based on a traditional tissue culture technique, plant stem cells are taken as a target, induction, separation and explant culture are conducted, and a corresponding stem cell culture system is established. By the adoption of the plant stem cell culture technique, the plant culture technique is enriched, and a novel research direction and opportunity is provided for commercialized production of natural products and development of the plant biotechnology.

The invention relates to a preparation method of a porous nano-fiber tubular scaffold, which comprises the steps that (1) PLLA (Poly L Lactic Acid) and other polymers are dissolved in a solvent to get a polymer solution; (2) the polymer solution is injected into a tubular mold and rapidly placed at a low temperature for phase separation, then the polymer solution is taken out, a tubular mold housing is removed, polymer gel after the phase separation and a core mold are immersed in ice water together, then the core mold is taken out, the polymer gel is immersed in deionized ice water to exchange the solvent, and the tubular scaffold is obtained; and (3) finally, the tubular scaffold obtained is frozen and dried for 48-120 hours, and then the porous nano-fiber tubular scaffold is obtained. The preparation method is simple to operate, and requires no additional hole-foaming agent, is suitable for volume production, and is lower in preparation cost; the prepared tubular scaffold has a nano-fiber structure similar to an extracellular matrix of a human tissue, and a porous structure with the diameter and porosity capable of being adjusted, and facilitates growth of cells and reconstruction of a cambium.

Compositions and methods are provided for treatment of cartilage defects in animals and humans. The compositions of the invention include synovial tissue, synovial cells and matrices containing synovial (or cambium) tissue or cells for use in filling a cartilage defect. The matrix and synovial tissue or cell preparations may also contain a proliferation agent, transforming factor or other active agents to promote healing. A controlled-release delivery system may be used to administer the transforming factor. The compositions of the invention also include a synovial covering membrane or devitalized fascial sheet for covering the cartilage defect. The methods of this invention are those in which a minimally invasive surgical intervention is performed to remove a small portion of synovial membrane from a joint. Portions of the synovial membrane, or cells expanded in vitro, are implanted alone or within a matrix, into the defect site, where they produce new cartilage tissue and repair the defect. Alternatively, partially transformed synovial-derived tissue may be formed in situ and implanted into the defect site.

The invention aims to provide a grafting method with minimum cut area for improving grafting survival rate, in particular a method for grafting different plants on the same plant at high survival rate and with simpleness, feasibility and wide application range. The grafting method comprises the following steps: (1) cion and parental stock selection, during which, an individual plant without disease and pest is selected as a parental stock and a wattle with the diameter similar to that of the parental stock is selected as a scion; (2) cion cutting, during which, 3 to 6 minutes of the scion is cut as a cion with one bud and one leaf (leafstalk only) left and the section perpendicular to the longitudinal direction of the wattle taken as a grafting surface; (3) parental stock cutting, during which, the parental stock is cut at the grafting height with the section perpendicular to the height direction of the parental stock taken as a grafting surface; (4) cion and parental stock combination, during which, the cion section and the parental stock section are tightly attached to align a cambium; and (5) interface binding and fixing, during which, an interface is fixed preliminarily by an adhesive tape and then the cion is tightly wrapped by a plastic film strip or a small-opening plastic bag so as to be fixed on the parental stock.

The present invention relates to a stem cell derived from cambium of family Gingkoaceae and to method for the isolated culturing thereof. The stem cell derived from cambium of family Gingkoaceae according to the present invention is advantageous as it is isolated at a non-differentiated state without passing through a dedifferentiation process and stably maintained without causing a variation in the cellular growth rate and growth pattern even during a long-term culture, thereby enabling a mass culture. In addition, the stem cell derived from cambium of family Gingkoaceae according to the present invention showed an antioxidant effect similar to or higher than that of existing synthetic antioxidants when the radical-scavenging activity for scavenging radicals generated by the treatment of an oxidant is measured, and thus can be valuably used as an excellent anti-inflammatory composition.

The invention discloses a grafting method of Magnolia wufengensis. A Magnolia denudate nursery-grown plant is taken as a rootstock, a current-growth branch of the Magnolia wufengensis is taken as a scion, and when the branch has a lignifying tendency in autumn, grafting can be started. The grafting method has the following steps: cutting an angular cut reaching xylem closely at the inner side of a phloem portion, then, performing longitudinal vertical sliding cutting downwards from right above the angular cut to the angular cut so as to cut a flat and smooth long tangent plane and ensure that the exposition position of the long tangent plane is mainly disposed on a cambium layer; performing sliding peeling downwards at the opposite side of a scion axillary bud to form a flat and smooth long-horse-ear-shaped tangent plane, wherein the tangent plane bridges over the diameter of the whole branch, a heart center is seen below the middle of the long-horse-ear-shaped tangent plane, and the axillary bud is sheared at a position 0.8 to 1cm away from the upper portion of the axillary bud; and carrying out scion colligation. According to the grafting method provided by the invention, the grafting butt joint surface is enlarged, the more callus a wound generates, healing can be speeded up, through such a means, the technical difficulty of rapid breeding of the Magnolia denudate is solved, and the technical defect is detected that the survival rate by use of a conventional Magnolia denudate grating method is low.

The invention provides a method for grafting fruit tree seedlings in spring. The method comprises the following steps of collecting scions, choosing grafting time, paring rootstock, paring the scions, jointing, cutting the rootstock, erasing sprouts, dressing top, removing bundling substances, and the like. The method is characterized in that the scions are collected at the period of 2 to 3 weeks before germination in spring and are sealed with wax on the bottom on standby; when the rootstock slightly buds, one smooth side exposed to the sun is processed with a downward flat cut with the length of about two centimeters, and the underside of the rootstock is processed with a crisscross cut, so as to form a small oblique plane; a position one centimeter above the scion bud is processed with a downward flat cut with the length of about two centimeters, and then the underside of the scion bud is processed with a crisscross cut, so as to form another small oblique plane; the scions are pressed on the rootstock, the cambiums of the rootstock and the scions are aligned; the rootstock is shorn off at a position two centimeters above the scion bud 10 to 15 days after grafting; when the scion bud germinates, the sprouts of the rootstock are erased; and when a seedling is 20 to 30 centimeters high, the grafting bundling substances are removed. The method solves the problem that the grafting propagation of forest fruits is low in survival rate and high in cost, and is applicable to the grafting propagation of fruit trees.

The invention relates to an asexual propagation method of michelia compressa. The asexual propagation method includes the steps that firstly, seedlings of the michelia compressa are manufactured; secondly, pretreatment of a scion of michelia compressa is performed; thirdly, a grafting method is adopted; fourthly, management after grafting is performed. Liriodendron chinense is used as a rootstock, and the cut-grafting method is used for grafting the michelia compressa. After cutting is sequentially soaked into a plant growth regulator and 1%DMSO, the rootstock is inserted, and after the cambium of the scion and the cambium of the rootstock are aligned, an anti-drop film is used for tightly winding and wrapping a port from bottom to top. According to the asexual propagation method of the michelia compressa, a great number of root-included seedlings of the michelia compressa can be obtained, the rate of reproduction is 81-86 times, and after the seedlings are obtained, the seedlings are grafted on the Liriodendron chinense, namely the rootstock, and the survival rate is 95%. After transplantation on a field is achieved for two years, the tree vigor is strong, a tree is resistant to diseases and capable of wintering safely, the problem of vacancy of the market resource of the michelia compressa is effectively solved, and the asexual propagation method of the michelia compressa is wide in application prospect.

The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Taxus cambium- or procambium-derived cell line; a lysate thereof; an extract thereof; or a culture medium thereof. The cell line, the lysate, the extract, and the culture medium has minimized side effects compared to the conventional therapeutic drugs, is safe to the human body, is involved directly in the growth of cancer to induce cancer cell death, and shows anticancer activity of inhibiting angiogenesis occurring in carcinogenesis. Accordingly, the cell line, the lysate, the extract and the culture medium is useful for the prevention, treatment and alleviation of cancer.

The invention discloses a method for improving the graft survival rate of a holly, which comprises the following steps: from the last ten-day of February to the middle ten-day of March and 3 to 5 days before grafting, irrigating a grafting nursery with water once thoroughly; scissoring a twig of a variety to be grafted, which grows robustly with good buds and has a thickness of between 1 and 2 centimeters as a scion, using a grafting knife to cut obliquely once at a 1-centimeter part on the back of a scion bud to cut off 1/3 of a xylem and ensure that the inclined plane is about 2 centimeters in length, then cutting a small inclined plane on the back side of the inclined plane to cut off certain xylem slightly and ensure that the bevel of between 0.5 and 0.8 centimeter in length, and scissoring off the scion at the position of 1 to 2 centimeters above the bud; planting a Chinese holly selectively (without considering the stem height and the thickness), using a lateral branch twig of which the thickness is between 1 and 2 centimeters as a rootstock, using a pair of lopping shears to shear at the position of 5 to 6 centimeters above a bifurcation, selecting a smooth position around the rootstock bark, and cutting vertically downwards for about 2 centimeters at the smooth place with a slight xylem; inserting the scion into a cut of the rootstock so that cambiums on two sides of the long inclined plane of the scion are aligned with and abutted against the cambiums on two sides of the rootstock, and if the scion is thin, the scion is ensured to be aligned with the cambiums on one side of the rootstock; fastening and sealing the cut by a plastic strip at an inserting opening from top to bottom; watering the interface with a watering pot, and sleeving a plastic bag on the cut so as to seal the cut and preserve moisture; building a shading net for sun-shading and temperature reduction at a position 2 meters above the nursery; and removing the plastic bag sleeved outside.

The invention discloses a nursery stock grafting method which comprises the following steps of (1) selection of a stock, selection of a scion, (3) combination, (4) treatment of the combined portion and (5) timely watering after combination. In the combination process, the combined part of the stock is cut open to form a 30-degree oblique plane through a disinfected knife, the phloem of the lower portion of the scion is cut off by 1 cm to 1.2 cm, so that the cambium is exposed, the cambium of the stock is combined with the cambium of the scion. The grafted nursery stock is managed in a conventional method. According to the nursery stock grafting method, the combination area between the cambium of the stock and the cambium of the scion is enlarged, combination is tighter, the grafting survival rate can be increased, the survival rate can achieve 80 percent to 90 percent, the germination rate of the nursery stock after grafting can be increased at the same time, and the nursery stock after grafting can survive ahead of time by 10 days to 15 days.

The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time. Accordingly, the cell line derived from the cambium of an herbaceous plant having a storage root makes it possible to produce large amounts of useful plants which are difficult to cultivate outdoor due to various problems associated with the period of cultivation, the selection of cultivation land, cultivation cost and the like.

The invention discloses an agarwood promoter and method for producing agarwood in Aquilaria sinensis. It is characterized in that the agarwood promoter is composed of following components in volume percentage: 5-10% of liquid cultivating Schizophyllum commune, 1.5-2% glucose or granulated sugar, 0.5-1% of acetate or malic acid, 85-95% of water. The method comprises the following steps: perforating Aquilaria sinensis trunk at a place not higher than 40cm through the bark, cambium, then perforating the secondary xylem, but hot hurting the primary xylem; introducing the agarwood promoter into the hole using a conduit and agarwood can be formed in half a year. The agarwood promoter of the invention has simple components and is easily acquired and prepared; the operation of the agarwood promoter to promote agarwood production is simple; the method of the invention has many advantages: obvious effect for effectively increasing agarwood productivity, shortening agarwood production period, better utilization and protection for Aquilaria sinensis resources.

The present invention relates to an anti-aging or antioxidant composition which contains, as an active ingredient, a plant stem cell line derived from the cambium of Panax ginseng, including wild ginseng and ginseng, or an extract thereof, a lysate thereof and a culture thereof. The composition has minimized side effects compared to existing anti-aging agents and antioxidants, and thus is safe for the skin. Also, the composition of the present invention shows an antioxidant effect of inhibiting reactive oxygen species caused by exposure to UV radiation that is the major cause of skin aging, and it can effectively reduce or inhibit aging-related factors. Thus, the composition is useful for the prevention and inhibition of aging.

The invention relates to application of ozone oil to an animal infectious disease. Ozone oil is applied to treatment of the animal infectious disease, and is smeared on the focus of the animal infectious disease till the animal infectious disease is cured and cambiums are generated. Ozone oil has strong sterilizing action, slow ozone release and long acting time. Oxygen atoms and grease released by the ozone oil can be used for promoting curing of a wound surface. When the ozone oil is used for treating the animal infectious disease, toxic and side effects on an animal are eliminated, the organs of the animal are not harmed, and a remarkable effect is achieved. According to the invention, the animal infectious disease can be eliminated, the healthy growth and reproduction of animals are promoted, the animal reproduction rate is increased, yield and income are increased for raisers, and virtuous circular development of livestock breeding is promoted.

Provided is a grafting method for kiwi berry trees. The method comprises: beveling from 1 cm below a scion bud eye in an angle of 45 DEG, beveling to 2/5 of the diameter of a scion branch, beveling downward from 1 cm above a bud to the depth of the first cut, taking down a bud sheet which slightly has a xylem and is totally 2-3 cm in length, then on a smooth position of a stock, 80 cm-100 cm above the ground, cutting apart the stock according to the method for cutting the bud sheet, and taking down cut blocks; then, embedding the bud sheet in the stock, the scion bud should be aligned at a cambium on one side of the stock, and finally, using a plastic strip to wrap tightly a grafting wound, to prevent moisture from losing. Since seedlings are high in survival rate, the survival rate of kiwi berries grafted by the method is more than 95%. The method greatly improves disease resistance, accelerates growth speed, improves yield, and changing variety is more convenient, just a top grafting position needs changing, and a whole plant does not need to be changed. When grafting in high places, planting and manual operation are more convenient.

Disclosed a new technology for fast growing seedling by grafting hard branch of grape, is characterized in the following method and steps : (1) building hotbed for growing seedling of grape and preparing heating means; (2) selecting high quality of hard branch for grafting and stock with developed and strong root; (3) shearing the stock in its 5-8 cm above the root, and making a vertical cut in the middle of said cut; cutting a surface with obliquity from 2.5 cm of lower graft to the bottom, and cutting a tangent plane in the back of graft at the base with two flower buds cutting in its upper part; introducing the oblique plane of graft into the stock along the cut with respect to at least one cambium of two sides, and packing securely the combination of the top part of stock and the grafting cut of the graft with plastic; (4) putting the seedling grafted on hotbed keeping a certain damp condition of root and a certain temperature condition around grafting combination, and the grafting combination will heal in twenty to thirty days therefore the seedling can be cultivated out. Said technology is in advantages of low cost, fast cultivation, novelty, easy graft, and survival rate than 95 %.

The invention discloses a slash pine resin tapping method for total healing of a tapping face. The method comprises the following steps: opening a median gutter in the center or at one side of the tapping face during first tapping, wherein the length of the median gutter is about 20-25cm and the width is about 1.5-2cm; tapping in the same year, namely after the first tapping, tapping from top to bottom along a tree trunk in sequence at an interval of 4-6 days, and spraying an irritant agent once to the tapping face in a vaporific manner every 12 days till the end of the resin tapping period of the year; and performing subsequent tapping in the next year, namely tapping according to the tapping method of the past year. The method is characterized in that the cutting depth of the median gutter ensures that a cambium layer of the tree trunk cannot be cut wound, so that the cambium layer of the tree trunk can be reserved undamaged; the subsequent tapping in the next year is performed at a position 1-2cm below a side gutter of the tapping face formed in the past year to cut a new tapping face. The method has the advantages that a biological and chemical reagent is used for stimulating pines to ensure that the pines form and excrete more rosin, the rosin flowing time is prolonged, the yield of rosin is improved, and meanwhile the tapping face can be completely healed in 2-3 years.

The present invention discloses a specific gene controlling growth of Arabidopsis vascular bundle and application thereof. The gene codes the protein of amino acid sequence shown in SEQ ID No.2 in sequence list and the genome gene is AT5g62940; the corresponding cNDA sequence is shown in the SEQ ID No.1 of the sequence list. As shown from research, the gene codes a transcription factor which is positioned in a core and has trans-activation activity, but the transcription factor which can regulate the formation of the Arabidopsis interfascicular cambium and the growth of vascular tissue is the transcription factor which is found as the first transcription factor for regulating the interfascicular cambium in Arabidopsis. In a transgenic gene plant, overexpression of the gene can thicken the stem of the plant, but inhibiting the expression of the gene in the plant can inhibit the occurrence of the interfascicular cambium. Therefore, the gene can be used for regulating the thickness of the stem, researching the woody plants with growth strength of secondary growth, and improving the quality. The gene has wide application prospect in regulating thickness growth and growth of the wood, flowers and plants.

The invention relates to a taxus mairei grafting culture method. The taxus mairei grafting culture method is characterized by comprising the following steps that (a) a 2-3-year old branch section with the light grey bark layer is cut out from a strong taxus mairei plant with needed characters, the bottom end of the branch section is cut with an art knife to form a long inclined face and a short inclined face which are symmetrical, and the bark layer of 0.4-0.5 cm is cut away from the upper end of the long inclined face to expose a cambium; (b) a common strong taxus mairei nursery stock is selected as a stock, and the smooth part on the shady face serves as a part to be grafted, wherein firstly, the knife edge cuts into the bark layer part in a 30-degree angle, the knife edge cuts downwards parallel to the stock after the white cambium is seen, and then downward cutting is continuously conducted by 2-3 cm with a 30-degree angle existing between the knife edge and the stock; (c) a scion is inserted into the notch of the stock through a left hand, the cambium at the upper end of the long inclined face of the scion is aligned with the cambium of the stock, the scion and the stock are pinched by a right hand, and a plastic thin film is used for sealing binding. The taxus mairei grafting culture method is good in grafting effect, high in survival rate and capable of rapidly culturing a large amount of nursery stocks.