82 results about "Slash Pine" patented technology

A method for somatic cell embryo generation and plant regeneration of a hybrid of a slash pine and a Caribbean pine is characterized in that the invention relates to a method which adopts a immature zygotic embryos of the hybrid of the slash pine and the Caribbean pine to carry out somatic cell embryo generation and plant regeneration and uses liquid and slide improved P6 mediums to alternatively combine for adjustment and control culture during the process of the somatic cell embryo generation and plant regeneration for a plurality of times and is appended with proper auxin combination. The invention provides a packaged technology for fast breeding the hybrid seedlings of the slash pine and the Caribbean pine industrializedly and can solve the current problem of short supply of the seedlings during the hybrid afforestation of the slash pin and the Caribbean pine.

The invention discloses a grafting method taking masson pine tissue culture seedlings as stocks. The grafting method comprises processes of stock cultivation, scion preparation, seedling grafting and post-grafting management and protection; by taking the masson pine tissue culture seedlings cultivated for 4 to 6 months as the stocks and collecting bud sticks germinating on stock plants as scions, grafting is performed by adopting a split grafting method in December to January or in March to April; grafted seedlings are cultivated under the conditions of suitable light and humidity, and water and fertilizer management and pest control are performed by a conventional method. According to the grafting method disclosed by the invention, the masson pine tissue culture seedlings with developed roots, vigorous metabolism and rapid growth are cultivated through a tissue culture technological means to serve as the stocks, therefore not only can the seedling period of the stocks be shortened and the grafting survival percentage be increased but also the genetic gain of an excellent variety is ensured; adverse effects brought by the fact that heterogeneous-stock grafting is performed by taking slash pines as the stocks widely in masson pine grafting in production at present are effectively avoided, and the working effect of masson pine tissue culture stocks is greatly promoted; the grafting method has a broad application prospect, and has better economic benefit, social benefit and ecological benefit.

The invention discloses an artificial pollination breeding method of a pinus massoniana hybrid. The artificial pollination breeding method comprises the following steps of 1, selection of masson pine as a female parent, 2, selection of pinus elliottii, pinus elliottii-pinus caribaea hybrid, pinus caribaea and pine moth-resistant pinus massoniana as male parents, 3, collection of male parent pollen, 4, treatment on the male parent pollen, 5, bagging and artificial pollination by a home-made pollination tool, and 6, cultivation. Through the artificial pollination breeding method, seeds of the pinus massoniana hybrid having the excellent and stable properties of straight trunk, fast early-stage growth rate, high rosin yield and pest and disease resistance are obtained. The artificial pollination breeding method solves the problems of open pollination in a pinus massoniana seed garden and improves crossbreeding efficiency. The artificial pollination breeding method is a complete artificial pollination breeding method, has simple processes, is practical, has a low cost, low requirements on operators, and small influences by a natural environmental factor such as weather, can effectively improve cross pollination efficiency in a pinus massoniana seed garden, can improve accuracy and reliability of cross pollination, can accelerate seed selection of a novel forestall germplasm resource variety, and has good economic, social and ecological benefits.

The invention discloses a method for shortening building time of a garden of Masson's pine clone graft seeds. According to the characteristic that slash pine is superior to Masson's pine in early growth, stocks of slash pine and Masson's pine are planted in a manner of two plantlets in one hole, and slash pine is grafted to Masson's pine in autumn and is supplementarily grafted in spring. Therefore, garden building time is shortened effectively while guaranteeing building quality, and the building efficiency of the Masson's pine clone graft seed garden is improved. The seed garden can be built for only 14 months by the method, which is 8 months smaller than the traditional building time. The efficient building method is economical, efficient, simple to operate, applicable to construction of bases for fine Masson's breeds and popularization to base production institutions, and practically significant to building of fine bases for Masson's pine.

The invention discloses a slash pine resin tapping method for total healing of a tapping face. The method comprises the following steps: opening a median gutter in the center or at one side of the tapping face during first tapping, wherein the length of the median gutter is about 20-25cm and the width is about 1.5-2cm; tapping in the same year, namely after the first tapping, tapping from top to bottom along a tree trunk in sequence at an interval of 4-6 days, and spraying an irritant agent once to the tapping face in a vaporific manner every 12 days till the end of the resin tapping period of the year; and performing subsequent tapping in the next year, namely tapping according to the tapping method of the past year. The method is characterized in that the cutting depth of the median gutter ensures that a cambium layer of the tree trunk cannot be cut wound, so that the cambium layer of the tree trunk can be reserved undamaged; the subsequent tapping in the next year is performed at a position 1-2cm below a side gutter of the tapping face formed in the past year to cut a new tapping face. The method has the advantages that a biological and chemical reagent is used for stimulating pines to ensure that the pines form and excrete more rosin, the rosin flowing time is prolonged, the yield of rosin is improved, and meanwhile the tapping face can be completely healed in 2-3 years.

The invention discloses slash pine needle extract. A preparation method of the slash pine needle extract comprises the following steps of shearing fresh slash pine needles by scissors to obtain slash pine needle sections with length of 2-3cm, crushing the sections by a high-speed crusher, adding water into the crushed pine needle sections according to a ratio of the crushed pine needle sections to the water of 1: (10-12), carrying out extraction for 24-26h, carrying out filtration by nylon gauze to remove residue, carrying out suction filtration by a vacuum pump to obtain a pine needle extract stock solution and carrying out dialysis purification to obtain the slash pine needle extract with different molecular weight ranges. The slash pine needle extract can be used for effectively inhibiting and killing moss in aquatic product culture, can improve water quality, has no toxicity, no harm, residue and pollution on the environment and other aquatic organisms, realizes prevention and control of moss in aquatic product culture and develops a novel application value and prospect of pine needles.

A functional compound natto health care product is compounded from natto, blueberry, slash pine needle, kidney bean, kudzuvine root and sorbitol. The compatibility proportions of the raw materials are as follows: 20 to 80 % of natto, 5 to 15 % of blueberry, 2 to 5 % of slash pine needle, 20 to 25 % of kidney bean, 15 to 20 % of kudzuvine root and 2 to 5 % of sorbitol, and the proportion of medicine and auxiliary material is (20 to 80 %) : (80 to 20 %). The health care product can be in the form of capsule, troche, chewable tablet, granula, oral liquid or soft capsule. The product has the health care effects of aging resistance, blood fat reduction, organism metabolism regulation, immunization improvement and the like, and has active social and economic significance on disease prevention, body-building and the realization of industrialization popularization.

The invention discloses a nutritional soil heavy metal restoration agent which is prepared from the following raw materials in parts by weight: 28-30 parts of fungus dreg, 16-19 parts of straw, 10-11 parts of wood dust, 7-9 parts of diammonium phosphate, 2-3 parts of sodium phytate, 5-7 parts of bentonite, 3-5 parts of medical stone powder, 80-90 parts of deseeded sunflower head, 28-32 parts of pig manure, 19-21 parts of kaolin, 8-9 parts of calcium carbonate, 28-30 parts of slash pine needle and a right amount of. The soil restoration agent can stabilize, enhance and balance the physical functions of the soil. The added fungus dreg, straw and the like increase the organic content of the soil. The medical stone powder, bentonite and the like enhance the air permeability of the soil, and relieve the soil hardening. The soil restoration agent has strong removal effects on chromium, cadmium and other pollutants in the soil, increases the soil fertility, and enhances the comprehensive properties of the soil.

The invention relates to a blueberry health-promoting steamed bun comprising the following materials by weight: 15-25% of blueberry, 3-10% of fresh slash pine needles, 15-25% of Sanqing bean flour, 35-55% of wheat flour, 0.5-3% of erythritol, and 0.1-2% of dry yeast. A preparation method comprises the steps of cleaning blueberry, and pulping to obtain blueberry juice; cleaning fresh slash pine needles, shearing into short pieces, adding water, pulping, and squeezing to obtain slash pine needle extractive solution; mixing dry yeast, Sanqing bean flour and wheat flour in a stirrer, adding erythritol, the blueberry juice and slash pine needle extractive solution, blending into dough, and making into steamed bun according to conventional method. The steamed bun has the advantages of fresh mouth feeling, rich nutrition, inhibition effect on mildew, prolonged shelf life, and health-promoting effect in enhancing immunity and preventing/treating diseases.

The invention discloses a photocatalysis soil heavy metal restoration agent, which is prepared from the following raw materials (by weight): 4-5 parts of titanium dioxide, 12-13 parts of egg shell, 5-6 parts of calcium ammonium nitrate, 6-8 parts of flyash, 10-14 parts of water hyacinth powder, 2-4 parts of potassium pyrophosphate, 85-95 parts of seeded sunflower disc, 40-45 parts of pig dung, 23-26 parts of kaolin, 12-14 parts of calcium carbonate, 32-35 parts of slash pine needle and a proper amount of water. By using organic biomass charcoal and organic fermented materials as adsorption carriers, lots of organic pollutants such as heavy metal ions and pesticide residue, etc. can be adsorbed on particle surface. After transmission light in soil catalyzes and restores titanium dioxide on particle surface, organic matter can be subjected to oxidative degradation, thus achieving the effect of thoroughly removing noxious substances in soil. The product has a good application prospect in the field of soil restoration.

The invention discloses a heavy metal contaminated soil restoration agent for wheat fields. The restoration agent is prepared from the following raw materials (by weight): 10-13 parts of barnyard manure soil, 2-3 parts of a Bacillus cereus microbial agent, 2-4 parts of a Rhizopus oryzae Went et Pr.Geerl. microbial agent, 3-4 parts of potassium phosphate, 25-28 parts of tree ash, 18-22 parts of fermented glutinous rice, 4-6 parts of ferric citrate, 86-94 parts of seeded sunflower disc, 15-20 parts of cornstalk, 10-14 parts of kaolin, 12-13 parts of calcium carbonate, 24-28 parts of slash pine needle and a proper amount of water. The Bacillus cereus microbial agent and the Rhizopus oryzae Went et Pr.Geerl. microbial agent which are added in the preparation of the soil restoration agent can coexist with original microorganisms in the soil to form new ecological balance. And under combined action of charcoal and a nutrient source, content of available heavy metals especially chromium in soil and content of heavy metals in wheat can be effectively reduced, thus being beneficial to growth and development of wheat. By the use of the restoration agent, quality of wheat is enhanced.

The invention discloses a method for preparing oligomeric rosin by taking slash pine rosin as a raw material. Preparation processes comprise the following steps of: melting the slash pine rosin into liquid-state rosin in a melting tank by heating; putting the liquid-state rosin into a reaction kettle and heating the liquid-state rosin; and extracting a substance with a low boiling point from the rosin under a vacuum condition. Under the vacuum condition, a vacuum degree is required to be at least -0.080 MPa. In a heating process, a catalyst in an amount which is 0.05 to 0.5 weight percent that of the rosin and a stabilizer in an amount which is 0.05 to 0.5 weight percent that of the rosin are added. The oligomeric rosin is prepared by polymerizing and stabilizing the slash pine rosin which serves as the raw material in vacuum at a high temperature. Without a solvent in the conventional method, the method has the characteristics of safe production and low cost. The main performance of the product of oligomeric rosin is moderate softening point, so that the oligomeric rosin is very suitable to be used as the raw material for a hot melt adhesive. Compared with the oligomeric rosin prepared by the conventional method, the product has lower acid value and lighter color.

The invention discloses a slash pine artificial forest transformation technology. The slash pine artificial forest transformation technology comprises the steps of slash pine forest stand selection standard, a forest stand survey, a single tree survey, forest stand transformation, protection measures and the like. Based on the status quo of slash pine timber forests, forest stands with a good forest stand soil productivity basis are selected, a scientific and reasonable transformation technology is adopted, a single timber wood target is replaced with a multi-purpose forest stand cultivation mode which integrates seedling growing superior tree protection, big-diameter wood cultivation and high-rosin-yield single-plant management, through broad-leaved trees under a forest, the soil productivity is renewed and maintained, the nutrient requirement for timber wood and rosin production is guaranteed, and therefore requirements for different production targets and different interests parties are guaranteed.

The invention discloses a tourmaline-assisted soil heavy mental restoration agent, which is prepared from the following raw materials (by weight): 12-14 parts of garden soil, 24-26 parts of sweet potato powder, 4-5 parts of sodium dihydrogen phosphate, 14-16 parts of tourmaline powder, 29-31 parts of peanut meal, 1-2 parts of nickel sulfate, 82-86 parts of seeded sunflower disc, 26-30 parts of manure of a pig farm, 20-24 parts of kaolin, 10-12 parts of calcium carbonate, 27-29 parts of slash pine needle and a proper amount of water. In allusion to heavy metal pollution and low bioavailability of persistent organic pollutants in soil, with assistance of organic materials such as tourmaline, peanut meal, sweet potato powder and the like, reproduction of soil microorganisms is promoted, bioavailability of pollutants is raised, and restoration efficiency of heavy metal and organic pollutants in soil by soil microorganisms is further enhanced. Pollutant content of restored soil is lower than the national standard. The product is suitable for promotion and application.

The invention discloses a full-functional soil heavy metal restoration agent, which is prepared from the following raw materials (by weight): 40-45 parts of rice chaff ash, 6-7 parts of ammonium dihydrogen phosphate, 10-12 parts of sawdust, 13-15 parts of lobster powder, 2-3 parts of silicon oxide, 95-100 parts of seeded sunflower disc, 30-35 parts of dried rabbit dung powder, 15-16 parts of kaolin, 6-7 parts of calcium carbonate, 32-36 parts of slash pine needle and a proper amount of water. The soil restoration agent has a significant improvement effect of heavy metal adsorption and passivation. By chemical bond combination of raw materials such as a charcoal matrix, silicon oxide, organic acid and the like, degradation ability of organic pollutants such as polycyclic aromatic hydrocarbon by indigenous microorganisms in soil can be greatly enhanced. With the addition of rice chaff ash and sawdust, etc., content of organic matter in soil is also increased, and soil quality is improved. The product is a full-functional restoration agent for comprehensive improvement of heavy metal pollution, organic pollution and chemical and physical properties of soil.

The invention discloses a rapid molecule detection method of Pinus massoniana Lamb. and Pinus elliottii, and a specific primer pair thereof. The primer pair is PJ164L/R and has nucleotide sequences which are represented by SEQ. ID. No.1 and SEQ. ID. No.2 respectively in a sequence table, and the primer pair allows a 303bp product to be specifically amplified in Pinus massoniana Lamb. and a 325bp product to be specifically amplified in Pinus elliottii. The rapid molecule detection method of Pinus massoniana Lamb. and Pinus elliottii is established depended on the strong specificity characteristic of the sequences, allows determination to be carried out through the once implementation of a PCR amplification reaction and gel electrophoresis directly according to the fragment of the product, has the advantages of simple operation, accuracy, reliability and high sensitivity, is suitable for the rapid and reliable molecule detection and identification of Pinus massoniana Lamb. and Pinus elliottii, and has great scientific research values to identify the Pinus massoniana Lamb. and Pinus elliottii hybrid seedling in the future.

A method of using slash pine containerized seedlings to directly renovate a metallic mineral waste yard comprises: (1), germinating slash pine seeds in seedling pots; (2) managing plantlets; (3) transplanting to degradable nonwoven container bags; (4) managing containerized seedlings; (5) preparing a mine mineral waste slope; (6) transplanting. The survival rate of slash pine seedlings grown with slash pine containerized seedling is high, the slash pine seedlings may be transplanted to the mine mineral waste yard to ecologically renovate the waste mine, the need of slash pine seedlings for growth nutrition can be met, the survival rate of slash pine in mineral waste is also increased, and site basis is provided for improving soil matrix of mine mineral waste yard in later stage and field planting of other renovating plants; the method of using slash pine containerized seedlings to directly renovate the metallic mineral waste yard provides a solution to the need of slash pine for growing on mine mineral waste sites.

The invention discloses an environmental-friendly restoring agent for heavy metals in soil. The environmental-friendly restoring agent is prepared from the following materials by weight: 15-28 parts of carboxymethyl chitosan, 17-31 parts of polyacrylamide,7-19 parts of polyaluminum chloride, 20-39 parts of polyaluminum ferric chloride, 14-31 parts of bentonite, 17-28 parts of sepiolite, 7-11 partsof calcium magnesium phosphate fertilizer, 28-33 parts of calcium superphosphate, 20-31 parts of magnesium sulfate, 15-27 parts of sodium silicate, 13-23 parts of boric acid, 21-31 parts of borax, 28-37 parts of zinc sulfate, 11-21 parts of amino acids, 9-14 parts of humic acid, 13-18 parts of a compound enzyme preparation, 4-6 parts of slash pine needles, 4-6 parts of Pseudomonas fluorescens powder, 7-11 parts of halotolerant yeast powder and 10-15 parts of Schizosaccharomyces pombe powder. The environmental-friendly restoring agent for heavy metals in soil can supplement a large amount of organic matters and nitrogen-phosphorus-potassium nutrient sources, reduce the activity of heavy metals in soil, improve the physical and chemical properties of soil, lower soil acidity, and achieve the purpose of restoring soil activity and fertility.

The invention provides a culture method for rooting slash pine x Caribbean pine F1 hybrid needle fascicles in water. The culture method includes the following steps: the needle fascicles of a slash pine x Caribbean pine F1 hybrid seed tree are taken and disinfected with carbendazim solution for 2 to 3 hours; the bases of the needle fascicles are cut; the bases of the needle fascicles are immersed in NAA solution for 12 to 16 hours, and the needle fascicles are washed with clean water for 2 to 3 times and vertically placed in glass bottles loaded with nutrient solution; the needle fascicles are cultured in a plastic greenhouse which is shaded by a shading net, and the nutrient solution is replaced once each week. By researching the affection of the age of the seed tree of the slash pine x Caribbean pine F1 hybrid needle fascicles, the concentration of growth regulator, the ingredients of the nutrient solution and other factors on the rooting of the needle fascicles, the invention obtains the optimal culture method for rooting the needle fascicles in water, and the rooting rate of the needle fascicles reaches 91.96 percent. The material source is rich, the operating method is simple, and is convenient to use, and the rooting rate is high. The operating process cannot pollute the environment, and the culture method is safe to use, and can be used for rooting needle fascicles of pine trees in water.

The invention discloses a secondary salinity soil heavy metal restoration agent, which is prepared from the following raw materials (by weight): 8-9 parts of lignite, 5-6 parts of weathered coal, 2-3 parts of potassium carbonate, 32-35 parts of cassava residue, 13-15 parts of malt sprout, 1-3 parts of manganese sulfate, 77-83 parts of seeded sunflower disc, 35-38 parts of silkworm excrement, 24-26 parts of kaolin, 9-10 parts of calcium carbonate, 29-31 parts of slash pine needle and a proper amount of water. In allusion to physicochemical properties of secondary salinity soil, lots of organic humus including lignite, weathered coal and the like are added to dilute soil salt and alkali content and enhance granular structure of soil. Meanwhile, by strong water absorption of biochar, water evaporation of soil can be reduced, accumulation of saline matter evaporated with capillary water towards surface soil is decreased, organic matter and soil microorganisms are increased, pH value of soil is reduced, and the ecological environment around soil is fundamentally improved.

The invention discloses a health-care jelly of red bayberry. The jelly is prepared from the material ingredients in percentage by weight: 10-25% of bayberry nuclear powder, 1.5-3% of pine needle juice of slash pine, 0.5-1.6% of jelly powder, 5-15% of bifidobacterium sugar, 0.05-0.2% of red bayberry essence, 0.2-0.9% of citric acid and the balance of water. The preparation technology comprises thesteps of: firstly, weighing the jelly powder and bifidobacterium sugar to put into a stirrer, adding water to agitate, heating and boiling when agitating to obtain an A product; adding the balance ofthe water in the ingredient to boil the A product; adding bayberry nuclear powder and pine needle juice of the slash pine when the A product is completely dissolved, reboiling and filtering by a filter cloth of 100-150 meshes, obtaining a B product; and adding red bayberry essence and citric acid, bulking, sterilizing and cooling the product after cooling to 70-80 DEG C to obtain a health-care jelly finished product of the red bayberry. According to the invention, since the health-care jelly product of the red bayberry is prepared from pure natural materials, characteristics of the existing jelly can be preserved, and the jelly can be prevented from generating mycete; a quality guarantee period is long; meanwhile, the health-care jelly has abundant nutritional ingredients and a healthcarefunction, the body can be built and the life can be prolonged after being eaten frequently.

The invention discloses an acidified soil heavy metal restoration agent, which is prepared from the following raw materials (by weight): 2-4 parts of quicklime, 2-3 parts of calcium sulfate, 6-9 parts of tobacco slag, 5-7 parts of iron chloride, 10-12 parts of monoammonium carbonate, 34-38 parts of coconut shell powder, 22-25 parts of charcoal, 3-4 parts of potassium sulfate, 88-92 parts of seeded sunflower disc, 24-26 parts of switchgrass branches and leaves, 25-28 parts of kaolin, 4-6 parts of calcium carbonate, 25-27 parts of slash pine needle and a proper amount of water. The soil restoration agent can be used for treating moderately acidified contaminated soil. With the addition of alkaline substances such as quicklime, calcium carbonate and the like, pH value of soil can be increased, and then microbial activities of soil are raised and decomposition of soil organic matter is accelerated. With matching of charcoal for adsorption of heavy metal cations, soil eluviation is decreased and soil fertility is increased. Thus, agriculture and forestry production of acidified soil is rapidly restored, and use value of soil is raised.