Artificial pollination breeding method of pinus massoniana hybrid

A technology of artificial pollination and masson pine, which is applied in the directions of botanical equipment and methods, application, and plant genetic improvement, can solve the problems of poor stem shape straightness, great influence of natural conditions, slow growth in the early stage, etc. Straight shape, low cost and rapid growth in the early stage

Inactive Publication Date: 2013-10-02
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the pollination in the masson pine seed orchards in operation is mainly free pollination by wind, which is greatly affected by natural conditions. If the pollen of the male flowers matures and looses pollen in rainy weather, the pollen cannot diffuse normally and combine with the female flowers, which will lead to abortion of the female flowers and cause the seed garden A large area of ​​production is reduced, which is very unfavorable for the selection of excellent strains of Masson pine
In addition, compared with foreign pine such as slash pine, wet plus pine and Caribbean pine, masson pine has poor straightness in dry form, slow growth in the early stage, and poor resistance to pine caterpillars, pine poisonous worms and other insect pests.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In March 2010, the improved generation seed garden of Pinus massoniana in the state-owned Paiyangshan Forest Farm in Guangxi Zhuang Autonomous Region was artificially controlled with Pinus massoniana as the female parent and Pine slash, Pine scum, Pinus caribbean and Pine-resistant pine caterpillar as the male parent. In the pollination cross experiment, a total of 141 combinations were pollinated. From October to November 2011, 131 combinations of hybrid masson pine seeds were harvested, and the remaining 10 combinations failed to pick cones due to branches broken by wind or man-made damage, with a total of 948.26 grams. Conventional methods were used to raise seedlings, and now Afforestation carried out progeny determination test, and the growth of seedlings was good.

Embodiment 2

[0028] In March, 2011, the supplementary test of embodiment 1 was carried out in the improved seed garden of Pinus massoniana in the state-owned Paiyangshan Forest Farm of Guangxi Zhuang Autonomous Region, and 90 combinations were pollinated in total. From October to November 2012, 90 combinations of hybrid masson pine seeds were harvested, totaling 155.44 grams. Conventional methods were used to raise seedlings. Now afforestation has been carried out for progeny testing, and the seedlings are growing well.

Embodiment 3

[0030] In March 2010, 47 Pinus massoniana were selected in the state-owned Paiyangshan Forest Farm of Guangxi Zhuang Autonomous Region to conduct artificial pollination hybridization experiments.

[0031] 1. Preparation before pollination

[0032] The masson pine with good growth and no pests and diseases was selected as the female parent of the plant, and the excellent individual slash pine with good growth and no pests and diseases in the stand was selected as the male plant, and the pollen of slash pine was collected in February. When collecting pollen, use branch scissors to cut 20cm-30cm well-grown branches of male flowers in the middle and upper part of the crown of the selected slash pine tree and bring them back to hydroponic culture. Collect pollen when the male flowers mature and loosen pollen. Put the collected pollen number of the male parent into a high-temperature sterilized brown glass bottle. At the same time, put an appropriate amount of desiccant in the bottl...

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PUM

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Abstract

The invention discloses an artificial pollination breeding method of a pinus massoniana hybrid. The artificial pollination breeding method comprises the following steps of 1, selection of masson pine as a female parent, 2, selection of pinus elliottii, pinus elliottii-pinus caribaea hybrid, pinus caribaea and pine moth-resistant pinus massoniana as male parents, 3, collection of male parent pollen, 4, treatment on the male parent pollen, 5, bagging and artificial pollination by a home-made pollination tool, and 6, cultivation. Through the artificial pollination breeding method, seeds of the pinus massoniana hybrid having the excellent and stable properties of straight trunk, fast early-stage growth rate, high rosin yield and pest and disease resistance are obtained. The artificial pollination breeding method solves the problems of open pollination in a pinus massoniana seed garden and improves crossbreeding efficiency. The artificial pollination breeding method is a complete artificial pollination breeding method, has simple processes, is practical, has a low cost, low requirements on operators, and small influences by a natural environmental factor such as weather, can effectively improve cross pollination efficiency in a pinus massoniana seed garden, can improve accuracy and reliability of cross pollination, can accelerate seed selection of a novel forestall germplasm resource variety, and has good economic, social and ecological benefits.

Description

technical field [0001] The invention relates to plant hybrid breeding, in particular to an artificial pollination breeding method for hybrid masson pine. Background technique [0002] Hybrid breeding is a breeding method that uses the same or different species of organisms with different genetic compositions to cross to obtain the desired phenotype type. The superior traits of the super-parents may, of course, also have a combination of inferior traits of the parents, or disadvantageous traits that the parents do not have. The breeding process is to select individuals that meet the breeding goals from among the many types of hybrid offspring for further cultivation until new varieties with excellent traits and stable quality are obtained. [0003] Masson pine is the main native afforestation tree species in southern my country. It has a wide distribution range and ranks in the forefront of the cultivated area. It has strong adaptability to climate and environment. Its main ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 王胤颜培栋杨章旗杨春霞冯源恒贾婕张明慧郭飞
Owner GUANGXI FORESTRY RES INST
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