126 results about "Pine tree" patented technology

The invention discloses a strain of arthrosporium spp. And application thereof in prevention and treatment of pine wood line disease. The invention relates to Arthrobotrys heuanensis 18GD1705A, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 15, 2021, the address of the Arthrobotrys heuanensis is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the postal code is 100101, and the preservation number is CGMCC No.23851. The Arthrobotrys heuanensis 18GD1705A has the advantages that the Arthrobotrys heuanensis 18GD1705A can be used for preparing a microbial inoculant; according to the method disclosed by the invention, the river-source arthrosporium is inoculated into pine seedling bodies, and the nematodes are caught by utilizing a three-dimensional viscous bacterium net formed by special hypha metamorphosis and can also be killed by forming a structure similar to a contraction ring through singular contraction. Meanwhile, before the pine wood nematodes colonize the pine trees, the river source arthrosporium inoculant is inoculated into the pine trees, so that the tree vigor of the pine seedlings can be effectively enhanced, and a better prevention effect is achieved.

The invention discloses development and application of a molecular marker for identifying a female parent of a pine tree. The technical problem to be solved is how to identify or assist in identifying the female parent of the pine tree. Specifically, the invention discloses application of substances for detecting SNP40, SNP81, SNP318, SNP443, SNP521 and / or SNP590 in identifying a female parent of a pine to be detected or preparing a product for identifying the female parent of the pine to be detected. The SNP40 is an SNP in pine mitochondria, is the 40th nucleotide of a sequence 1, and is C or T; the SNP81 is an SNP in pine mitochondria, is the 81st nucleotide of a sequence 1, and is A or C; the SNP254 is an SNP in pine mitochondria, is the 254th nucleotide of a sequence 1, and is C or T; the SNP318 is an SNP in pine mitochondria, is the 318th nucleotide of a sequence 1, and is A or C; the SNP443 is an SNP in pine mitochondria, is the 443rd nucleotide of a sequence 1, and is G or T; the SNP521 is an SNP in pine mitochondria, is the 521th nucleotide of a sequence 1, and is G or T; the SNP590 is an SNP in pine mitochondria, is a 590th nucleotide of a sequence 1, and is A or C. The source of a female parent to be detected is determined according to the sequence, and the invention can be used for industrial and agricultural production.

The invention discloses arthrobotrys guangdongensis and application of the arthrobotrys guangdongensis in prevention and treatment of pine wood nematode diseases. The invention relates to Arthrobotrys guangdonensis 20GD2519A, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 15, 2021, the address of the Arthrobotrys guangdonensis is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the postal code is 100101, and the preservation number is CGMCC No.23849. The Arthrobotrys guangdonensis 20GD2519A has the advantages that the Arthrobotrys guangdonensis 20GD2519A can be used for preparing a microbial inoculum; the invention directly aims at pathogenic nematodes, inhibits and kills nematodes, and reduces nematode density. The strain has strong sporulation ability, can maintain good hereditary stability, and still has high fatality rate to pine wood nematodes after continuous subculture. The survival rate of pine trees inoculated with the arthrobotrys guangdongensis suspension in advance can reach 72%.

The invention provides application of a pseudomonas chlororaphis strain Rhs-P27 in prevention and treatment of pine wood nematode diseases, and relates to the technical field of agricultural environmental microorganisms. In particular to a Pseudomonas chlororaphis strain Rhs-P27 separated from rhizosphere soil of Chinese pines and application of the Pseudomonas chlororaphis strain Rhs-P27 in prevention and treatment of pine wood nematode diseases. The strain is preserved in the China Center for Type Culture Collection in Wuhan University on December 18, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) 20242846. The in-vitro poisoning activity of the strain Rhs-P27 on pine wood nematodes can reach 100% within 24 hours; a fermentation filtrate and a zymophyte suspension of the strain Rhs-P27 have a remarkable inhibition effect on the propagation of the pine wood nematodes; the prevention effect of a zymophyte suspension of the strain Rhs-P27 on the pine wood nematode disease of biennial pinus tabulaeformis potted seedlings can reach 45%, and the density of pine wood nematode in pine trees can be remarkably reduced; the pseudomonas chlororaphis strain Rhs-P27 disclosed by the invention has a good application prospect in the field of pollution-free prevention and treatment of pine wood nematode diseases.

The invention provides a pine wood nematode disease tree detection method and system based on image analysis, and relates to the technical field of image processing. The method comprises the following steps: acquiring a to-be-analyzed pine image acquired by an unmanned aerial vehicle, and determining a plurality of reference crown areas in the to-be-analyzed pine image; respectively carrying out diseased tree fading analysis and crown form analysis on each reference crown area, carrying out primary anomaly detection on the plurality of reference crown areas, and dividing to obtain a reference sample group and an abnormal sample group; performing clustering analysis based on environment distribution difference on the plurality of reference tree crown areas in the reference sample group to generate a plurality of environment influence tree crown groups; and determining an environment influence crown group to which each reference crown region belongs in the abnormal sample group, and carrying out secondary anomaly detection on the plurality of reference crown regions to obtain a pine wood nematode pest detection result of the to-be-analyzed pine image. According to the invention, precise detection of the pine wood nematode disease tree is realized.

The invention provides a pine thread pest monitoring system and method based on an intelligent sensor. The method comprises the following steps: firstly, acquiring near infrared spectrum information of each pine tree sample through an intelligent spectrum sensor; further determining a characterization wave band of each pine tree sample; further determining the erosion state of each pine tree sample, and determining the identification quantity of each pine tree sample during pest grading through all erosion states and the characterization wave band of each pine tree sample; then, according to the similarity characteristic quantity of the near infrared spectrum image collected by each intelligent spectrum sensor and the humidity gradient at different sampling points, determining the recognition gain when performing pest qualitative treatment on each pine tree sample; and further determining the erosion trends of the pine nematodes at different sampling points in the pine nematode influence area, and sending all the erosion trends to a pine nematode pest monitoring center. By adopting the scheme of the application, the propagation trend of insect attack behaviors can be monitored at the initial stage of pine nematode infection, so that the accuracy of predicting the diffusion trend of pine nematode at the initial stage is improved.

The invention provides an abnormal color-changing pine monitoring method and system, and particularly relates to the technical field of monitoring and evaluation.The method comprises the steps that firstly, an unmanned aerial vehicle remote sensing image of a target forest area is obtained, and a preprocessing data set containing abnormal color-changing pine and withered pine labels is generated through clipping, framing and labeling processing; the data set is input into a target detection model for training, the model comprises a feature extraction layer based on a lightweight convolutional network, an attention mechanism layer integrating a channel and a space attention sub-layer, and a feature fusion layer adopting a bidirectional weighted feature pyramid structure, and finally a pine tree state recognition model is obtained. Based on pine tree coordinate data output by the model, multi-factor weight collaborative analysis is carried out in combination with multi-source environmental factors, a pine wood nematode disease diffusion risk probability graph is generated, and monitoring and early warning information is output according to the pine wood nematode disease diffusion risk probability graph. And efficient and accurate pine tree disease monitoring is realized.

The utility model belongs to the technical field of forestry epidemic disease detection, and discloses a sampling device for pine wood nematode disease detection, which comprises a U-shaped frame, one side of the U-shaped frame is fixedly connected with a support, one end of a push rod is fixedly connected with a push plate, the other end of the push rod is fixedly connected with a motor, the tail end of a rotor of the motor is coaxially and fixedly connected with a drill bit, and the drill bit is fixedly connected with the support. The clamping assembly comprises a one-way lead screw rotationally connected to one side of the U-shaped frame, the outer portion of the one-way lead screw is in threaded connection with a moving block, clamping plates are symmetrically arranged on the inner side face of the U-shaped frame, a cross rod is fixedly connected to one side of each clamping plate, and the cross rods are in sliding connection with the U-shaped frame; by means of the pine tree sampling device, the problems that due to the fact that the stability during drilling and sampling is difficult to guarantee, the pine tree sampling efficiency is reduced, and damage to pine trees is easily increased are effectively solved.

The attractant is composed of (1R)-2, 2-dimethyl-3-methylene dicycloheptane, pinene, camphene and other components, 95% ethyl alcohol is used as a solvent, porous silica gel microspheres with the average pore size of 2-5 nm are matched to serve as a slow-release carrier, and the pine tree longicorn attractant is prepared. The preparation method comprises the steps of step-by-step mixing, ultrasonic dispersion, standing reaction and slow-release packaging, and process parameters of each step are accurately controlled. Compared with the prior art, the pine tree longhorn beetle attractant has the remarkable advantages that through precise matching of the components and collaborative optimization of technological parameters, triple breakthrough of the pine tree longhorn beetle attractant in targeting, stability and cost control is achieved, and an efficient and reliable technical scheme is provided for green prevention and control of pine wood nematode diseases.

The utility model discloses improved pine cultivation equipment which comprises a cultivation frame, a plurality of basin fixing hoops are installed on the cultivation frame, cultivation basins are placed in the basin fixing hoops, a water guide drawer is arranged below the cultivation basins, and erecting columns are arranged on the two sides of the water guide drawer. A pot body is arranged in the culture pot, connecting pipes are installed on the two sides of the pot body, an air conveying disc is arranged between the connecting pipes, air holes are formed in the outer surface of the pot body, and a plurality of drainage holes are formed in the bottom of the pot body. Pine seedlings are placed in a culture pot, in order to improve pine and grow vigorously, an oxygen supply device can be connected to an input cylinder, a hand wheel is controlled by the input cylinder to rotate, oxygen is input into an air conveying disc from a connecting pipe, oxygen is supplied into soil from a guide column, and therefore the roots and stems of the pine seedlings can absorb oxygen, and the roots and stems of the pine seedlings can grow vigorously. And meanwhile, the other connecting pipe can be connected with a medicament, and the medicament is sprayed between the guide columns, so that the medicament can be absorbed by the soil more sufficiently, and the culture effect of the pine wood is further improved.

The invention relates to the technical field of agricultural microorganisms, in particular to bacillus tequilensis R78 for preventing and treating pine wood nematode diseases and application of the bacillus tequilensis R78. The bacillus tequilensis R78 for preventing and treating the pine wood nematode disease is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32106, and the preservation time is September 29, 2024. The bacillus tequilensis R78 can be used for preventing and treating the pine wood nematode disease. The bacillus tequilensis R78 is separated from pine trees, and fermentation liquor of the bacillus tequilensis R78 has stable and efficient pine wood nematode inhibiting and killing capacity. When OD600 is equal to 1.5, the fatality rate of fermentation liquor of the strain R78 on pine wood nematode can reach 95% or above, the disease index of pine tree infected with pine wood nematode disease can be remarkably reduced, and the prevention and treatment effect can reach 68.6%.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a promoter of a pinus massoniana terpene synthase gene Pm TPS (-)-alpha-pinene and application of the promoter. According to the invention, a promoter fragment of a Pm TPS (-)-alpha-pinene gene and three homeopathic action elements with jasmonic acid response on the promoter fragment are obtained from pinus massoniana. A complete promoter fragment of the Pm TPS (-)-alpha-pinene gene of the pinus massoniana is transformed into arabidopsis thaliana by means of agrobacterium mediation, and the promoter can drive expression of a GUS gene in leaves of the arabidopsis thaliana or nicotiana benthamiana. GUS staining and GUS enzyme activity of transgenic Bensi tobacco leaves are reduced along with deletion of jasmonic acid response cis-acting elements on the promoter. Furthermore, the yield of pine resin terpenoids can be increased by utilizing the promoter and the homeopathic element thereof.

The invention relates to the technical field of biology, in particular to a pine bark polyphenol extract as well as a preparation method and application thereof. According to the present invention, the pine tree bark polyphenol extract is obtained through extraction with an ethanol solution, and the pine tree bark polyphenol extract mainly comprises 2-Hydroxy-4-hydroxybenzoic acid, Pinosylvin, Hispidulin, 3, 5-Dimethoxyphenol, 2, 3, 5, 4 '-Tetrahydroxystilbene, 2-O-beta-D-glucose, Quercetin-3-O-glucose, 6-O-Caffeoyl-D-glucose, Galvantinol, MeIAA and Rhapontigenin, and the pine tree bark polyphenol extract can be used for preparing the pine tree bark polyphenol extract, and can be used for preparing the pine tree bark polyphenol extract, the pine tree bark polyphenol extract, the pine tree bark polyphenol extract, the pine tree bark polyphenol extract, the pine tree bark polyphenol extract, the pine tree bark polyphenol extract and the preparation method thereof, the pine bark polyphenol extract can be used for effectively relieving LPS (lipopolysaccharide)-induced intestinal barrier injury of weaned piglets by regulating intestinal flora (improving Pediococcus abundance and reducing Alloprevotella abundance) and a tryptophan-AhR pathway under the condition that the addition amount of the pine bark polyphenol extract is lower than 0.05 wt%.

The utility model provides a kind of for Pinus taeda growth environment monitoring device, belong to soil monitoring equipment technical field, the device includes support plate, fixed monitoring mechanism, fixed block, monitoring cabinet, water tank, water inlet bucket and apron, in use, monitoring probe is connected in fixed clamping plate, data transmission line is set in slot simultaneously, then insert pipe is inserted into land, monitoring probe is sent into ground bottom by insert pipe, to carry out real-time monitoring to underground soil environment, insert pipe can fix the whole device simultaneously, it is convenient to fix installation monitoring device on sand, further improve the stability of the whole device fixed by cooperation support plate and support leg, in addition, rainwater can enter water inlet bucket by multiple water inlets on apron, rainwater can be collected and stored by water tank, it is convenient to carry out irrigation work, meanwhile, water inlet bucket, connecting pipe and apron can reduce the transpiration loss of water under high temperature environment.

1. Name of the designed product: concrete barrier (pine tower type). 2. Use of the designed product: barrier for road safety protection. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The utility model relates to the technical field of medicine bottle recycling equipment, and discloses a pine tree punching and medicine injection medicine bottle recycling device which comprises a supporting ring and a collecting net, the collecting net is installed at the top of the supporting ring, a forking mechanism and an adjusting mechanism are arranged on the outer side of the supporting ring, and the forking mechanism comprises a connecting block, a supporting block, a fork rod and a first bolt. The two sets of supporting blocks are rotationally connected to the inner side of the connecting block, one end of each fork rod is welded to the outer side of the corresponding supporting block, and the first bolt penetrates through the connecting block and the supporting blocks. According to the pine punching and medicine injection bottle recycling device, a fork rod is inserted between a medicine bottle and a pine and forks the medicine bottle out of the pine, so that the pine punching and medicine injection bottle can be conveniently recycled into a collecting net, meanwhile, an operator can pull the fork rod, the fork rod drives a supporting block to rotate on the inner side of a connecting block, and therefore the angle of the fork rod is changed; and the recovery device can conveniently recover medicine bottles with different sizes.

The invention discloses a pine wood nematode disease rapid detection method, and relates to the field of forestry plant quarantine and pest and disease damage detection, a portable integrated detection device is used for executing the following steps: S1, sample collection: a miniature drilling unit of the device automatically drills into a to-be-detected pine tree trunk to take a wood chip sample; s2, sample pretreatment: conveying and weighing a wood chip sample, transferring the wood chip sample to a crushing and extracting chamber after the wood chip sample reaches a target weight, adding a lysis solution, heating, crushing and extracting to obtain an extracting solution; s3, sample transfer: injecting the extraction liquid into a micro-fluidic chip mechanism by using a diaphragm pump; s4, nucleic acid purification and isothermal amplification: carrying out nucleic acid purification on the extract in a micro-fluidic chip mechanism, and carrying out a nucleic acid isothermal amplification reaction at a constant temperature; and S5, optical detection and result analysis: carrying out real-time fluorescence monitoring on a nucleic acid isothermal amplification process, obtaining and processing a fluorescence signal, calculating the estimated concentration of the pine wood nematodes based on an algorithm model, and evaluating an infection risk level in combination with forest stand background information. The method realizes on-site rapid and accurate detection, and can meet field rapid detection requirements.

The utility model belongs to the technical field of deinsectization equipment, and particularly relates to an electric reeling and killing device for pine tree trunk borers, which comprises a dredging spring, the dredging spring is provided with a dredging head, the dredging head is provided with a groove, the groove is provided with a through hole, an extension rod is slidably arranged in the through hole, a hookworm block is fixed on the extension rod, the extension rod is provided with a stop block, and a cavity is provided with a trigger assembly. The dredging spring is provided with a mounting head; an electric drill is started, the electric drill drives a dredging spring and a dredging head to rotate and advance and go deep into the end of a worm hole of a tree trunk, in the advancing process, all stop blocks are pushed to move all around by means of a trigger assembly, all stop blocks drive an extension rod and a hookworm block to move all around, and after injurious insects encountered along the way are caught and killed, the injurious insects are hooked by the hookworm block; the device is pulled out of the tree hole along with the dredging spring, so that the pine tree trunk borer pests are quickly caught and killed in a physical prevention and control mode, the cost for purchasing a large amount of pesticides and the labor cost for spraying the pesticides are saved, environment pollution and human body damage can be avoided, and the device is environmentally friendly and efficient.

The present invention relates to the technical field of pine hybridization. The present invention provides a method for distant hybridization of the genus Pinus based on stock-scion interaction, comprising the following steps: (1) selecting stock and scion of different tree species for grafting to obtain a grafted plant; (2) cultivating the grafted plant, removing the stamens and bagging before the female flowers of the grafted plant turn white; (3) after the female flowers of the grafted plant turn white, artificial pollination is performed using pollen of a pine tree of the same variety as the stock. The present invention uses pine trees of different factions for grafting and hybridization, and utilizes the horizontal transfer of small molecules between the stock and scion to achieve the possibility of interspecific hybridization, thereby overcoming the problem of highly incompatible hybridization between different subgenera of pine trees.

This invention discloses a method for establishing a spectral characteristic database of trees affected by wood nematode, involving the interdisciplinary fields of forestry disease monitoring, spectral analysis, and database technology. By capturing subtle spectral changes in pine needles in the red and near-infrared bands, this invention can identify signs of infection before obvious symptoms appear on the tree's surface. Through a multi-step, multi-index comprehensive analysis method, it effectively eliminates interference from other ground features such as forest land and bare soil. The established dedicated spectral model and indices are highly targeted, achieving higher classification and identification accuracy than using a single general index, significantly reducing the false positive rate. The use of UAV hyperspectral remote sensing technology overcomes the shortcomings of manual inspection, such as limited coverage, numerous blind spots, and poor timeliness, enabling rapid and comprehensive monitoring of large-scale pine forest resources. It can not only determine whether pine trees are infected but also quantitatively assess the severity of the disease through chlorophyll content estimation models and spectral characteristic differences.

The utility model relates to the technical field of decorative articles, in particular to a five-fork double-color PE simulation Nobel pine tree. The five-fork double-color PE simulation Nobel pine tree solves the technical problem that a simulation tree in the prior art cannot be dynamically displayed. A five-fork double-color PE simulation Nobel pine tree comprises a base. The tree trunk is arranged on the base; the decorative tree leaves are arranged on the tree trunk; by arranging the driving assembly, the driving assembly can drive the inner rod and the outer rod to rotate, so that the simulated Nobel pine tree can achieve a dynamic display effect, and the decoration performance of the simulated Nobel pine tree is optimized. Meanwhile, the reversing assembly is adopted, the inner rod and the outer rod can have different rotation directions through the reversing assembly, or the inner rod and the outer rod have different rotation speeds through the reversing assembly, and the decoration performance of the simulated Nobel pine tree is further optimized.

This invention provides a comprehensive fertilization method to promote the rapid growth of Podocarpus macrophyllus, including step S1: Before planting the Podocarpus macrophyllus, determine the amount of fertilizer, the depth of the planting hole, and ensure that the diameter of the planting hole is larger than the diameter of the root ball of the Podocarpus macrophyllus, based on the diameter of the pine tree's rootstock. Step S2: Mix the fertilizer combination that meets the required amount of fertilizer with the soil excavated from the planting hole to form a soil-fertilizer mixture. When planting the Podocarpus macrophyllus, use the soil-fertilizer mixture to bury the root ball of the Podocarpus macrophyllus in the planting hole. Step S3: During the growing season of the Podocarpus macrophyllus, use a combination of short-acting and long-acting fertilizers to promote its rapid growth. This invention can provide optimal fertilizer efficiency for Podocarpus macrophyllus and promote its rapid growth.

The invention relates to the technical field of tree protection, and particularly discloses a composite agent for preventing and controlling pine trunk borers and a use method of the composite agent. The invention discloses a composite medicament for preventing and controlling pine trunk borers. The composite medicament comprises a component A and a component B, the component A is prepared from the following raw materials: emamectin benzoate and an emulsifier; the component B is prepared from the following raw materials: an emulsifying agent, an adhesive, cypermethrin and emamectin benzoate. According to the application, the component A consisting of the emamectin benzoate and the emulsifier and the component B consisting of the emulsifier, the adhesive, the cypermethrin and the emamectin benzoate are matched for use, so that the prevention and control effect on the monochamus alternatus and the monochamus alternatus is improved, and the healthy growth of pine wood is promoted.

The invention discloses a pesticide injection device for pine nematode disease prevention and control, and relates to the technical field of pine nematode disease prevention and control, the pesticide injection device comprises a supply box, a drilling machine, a measuring rod and a drilling rod, the drilling machine is connected with a wire, the other end of the wire is connected with the supply box, a battery is installed in the supply box, and pesticide is stored in the supply box; a mounting box is fixedly connected to the upper end of the end, away from the drilling machine, of the base, a driving mechanism and a medicine supply mechanism are arranged in the mounting box, a sliding groove is formed in the side wall of the end, away from the drilling machine, of the mounting box, one measuring rod is fixedly connected with the mounting box, the other measuring rod is fixedly connected with a sliding block, the sliding block is in sliding connection with the sliding groove, and the drill rod is slidably sleeved with an indicating rod. The pine tree pesticide injection device has the advantages that pesticide injection can be directly conducted on pine trees after punching is conducted, the pesticide injection efficiency is remarkably improved, the punching depth and the pesticide injection amount can be accurately controlled according to pine trees with different diameters, and the control effect on the pine tree nematode disease is improved.

1. Name of the Design Product: Coat Rack (J2 Pine Tree). 2. Use of the Design Product: The design product is a coat rack and can be used for daily use. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Indicates the Design Key Points: Stereogram.

1. Name of the designed product: upper garment (rice field pine tree tent). 2. Use of the designed product: for wearing. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: front view. 5. The designed product is a thin product, so the left view is omitted; the designed product is a thin product, so the right view is omitted; the designed product is a thin product, so the top view is omitted; the designed product is a thin product, so the bottom view is omitted.

The present invention relates to a method for rapidly and efficiently extracting a plant essential oil from a plant raw material, particularly from xylem of a pine tree, thereby maximizing the yield, reducing the manufacturing cost and time, improving the productivity, performing high-purity treatment on the extracted plant essential oil by a refining process, thereby realizing the diversification and commercialization of the extracted oil, and improving the quality of the plant essential oil. The invention relates to a plant essential oil extraction method and plant essential oil extracted oil extracted by the extraction method.

1. Name of the product in this design: Baijiu Box (Welcoming Pine Tree Box). 2. Purpose of this design: For holding wine bottles, etc. 3. The key design elements of this product are the combination of shape, pattern, and color. 4. The image or photograph that best illustrates the design's key points: 3D view 1. 5. The design for which protection is sought includes color. 6. The bottom view and the top view are symmetrical, so the bottom view is omitted.

1. The name of the design product: lamp ornament (pine tree). 2. The use of the design product: the design is used for lighting and decorative lamp ornaments. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.