Burkholderia multivorans WS FJ9 and application thereof to growth promotion of pine

A polyphagic Burkholderia and Holderia technology, applied in the field of microorganisms, to achieve the effect of promoting growth and development, significant differences, and excellent strain resources

Active Publication Date: 2012-07-25
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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At present, there are few reports on the screening and application of h

Method used

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  • Burkholderia multivorans WS FJ9 and application thereof to growth promotion of pine
  • Burkholderia multivorans WS FJ9 and application thereof to growth promotion of pine
  • Burkholderia multivorans WS FJ9 and application thereof to growth promotion of pine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The phosphorus solubilizing ability test of embodiment 1WS-FJ9 bacterial strain:

[0026] Phosphate Solubilizing Medium A: Glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 5g, KCl 0.2g, MgSO 4 .7H 2 O0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, pH 7.0.

[0027] Phospholytic medium B: with AlPO 4 Instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , the other components and contents are the same.

[0028] Montkina organophosphate medium, used for the liquid culture of organophosphate-decomposing bacteria for the determination of phosphorus-dissolving ability: glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, CaCO 3 5g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, lecithin 0.2g, distilled water 1000mL, pH7.0~7.5.

[0029] Inoculate the activated WS-FJ9 strain into NB medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of distilled water, pH 7.2-7.4), shake culture at 30°C for 18-24h to make seed...

Embodiment 2

[0032]Example 2 WS-FJ9 bacterial strain optimum phosphorus solubilizing condition test:

[0033] After WS-FJ9 is activated, inoculate into NB medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of distilled water, pH 7.2-7.4), shake culture at 30°C for 18-24h to make seed liquid, and take 0.5mL of seed solution were respectively inoculated in 100mL Erlenmeyer flasks equipped with 50mL of NBRIP (Phosphate Growth Medium of the International Botanical Research Institute, formulated with phosphate-dissolving medium A) as the basic medium, and the carbon source of the NBRIP medium was respectively set to sucrose, fructose , maltose, lactose, and mannitol; the nitrogen source was set to peptone, beef extract, potassium nitrate, ammonium nitrate, and calcium nitrate, respectively, and the phosphorus-solubilizing medium connected with the same volume of blank seed solution was used as the control (CK), and each treatment had 3 Repeat, 30°C, 180r / min shaking cultu...

Embodiment 3

[0034] Embodiment 3WS-FJ9 bacterial strain greenhouse pot test:

[0035] After WS-FJ9 was activated, a small amount of bacterial cells were picked with an inoculation loop and inoculated into a 100 mL Erlenmeyer flask containing 50 mL of NB medium (the formula is the same as that in Example 1), and cultured at 29°C and 180 r / min for 72 hours with shaking. Centrifuge the fermentation broth (4°C, 6000r / min) for 5min, rinse the bacteria with sterile normal saline for 2 to 3 times, then adjust the bacterial suspension with sterile normal saline (7~8×10 8 cfu / mL) to make WS-FJ9 bacterial agent. Pine slash seedlings (120 days old) were inoculated, and the same amount of sterile saline was used as the control (CK), and the inoculation volume was 15 mL / plant. 20 replicates per treatment were placed in the greenhouse for unified management, the light was 12h / d, and watering was timely.

[0036] The growth situation of slash pine seedlings inoculated 180d, the results are shown in Tab...

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Abstract

The invention discloses burkholderia multivorans WS FJ9 which is separated from the soil at the root of the pine and can dissolve the slightly-soluble inorganic phosphorus and organic phosphorus efficiently and the application of the burkholderia multivorans. The WS FJ9 burkholderia multivorans is collected in the CCTCC (China Center for Type Culture Collection) on December 1, 2011 and has a collection number of No. M2011435. The burkholderia multivorans WS FJ9 has strong tricalcium phosphate, aluminum phosphate and lecithin dissolving effect under the condition of submerged culture, is very different from the contrast, has strong sphaeropsissapinea inhibiting activity and can be prepared into a microbial inoculum inoculated to the wetland pine to promote the growth of the pine. The invention provided an excellent strain resource for the development of the biological fertilizer for the pine.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a pine rhizosphere high-efficiency phosphorus-solubilizing bacterium Burkholderia polyphagia and its application in promoting the growth of pine trees. Background technique [0002] Phosphorus is one of the three major elements of plant nutrition. It is an important component of nucleic acid, various enzymes, and coenzymes in plants. Metabolism plays an important role, and phosphorus deficiency can lead to stagnation of plant growth and development, poor root development, dwarf plants, and severe decline in biomass. Therefore, phosphorus is an essential nutrient for plants. [0003] Soil contains a large amount of reserve phosphorus, which can be divided into inorganic phosphorus and organic phosphorus, but mainly exists in insoluble form. Phosphorus in the soil is mainly fixed by three methods: adsorption, precipitation, and bio-holding. More than 95% of the phosp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08A01N63/00A01P21/00A01P3/00C12R1/01
Inventor 吴小芹侯亮叶建仁李冠喜
Owner NANJING FORESTRY UNIV
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