32 results about "Masson pine" patented technology

The invention discloses a method for efficiently preparing fulvic acid. The forestry waste is smashed and sieved, the pH is adjusted to be acidic, and a pretreatment solution is obtained after ultrasonic treatment; the preparation method comprises the following steps: dissolving Fe (NO3) 3.9 H2O in deionized water, adding gamma-Al2O3, carrying out ultrasonic treatment, adding EDTA-2Na, stirring until the solution is clear, filtering, and drying to obtain a composite catalyst; adding an H2O2 solution and a composite catalyst into the pretreatment solution, mixing, heating and stirring for reaction, adjusting the pH to be alkaline, standing and filtering to obtain supernate; and drying the supernate to constant weight, grinding and sieving to obtain the fulvic acid. According to the method, the masson pine sawdust is used as a raw material, and the EDTA-Fe / Al2O3 composite catalyst and H2O2 are subjected to oxidation reaction, so that the yield, the purity and the carboxyl content of fulvic acid are remarkably improved. The method can be used for soil improvement, organic fertilizer production and environmental governance, and has remarkable ecological and economic values.

The present invention belongs to the technical field of specialty paper preparation, and specifically relates to a method for producing specialty paper. The method for producing specialty paper of the present invention uses a compound of bleached sulfate masson pine pulp and bleached wheat straw pulp to construct a three-dimensional skeleton structure in which long and short fibers are interwoven, and uses modified calcium titanate whiskers, modified magnesium borate whiskers and polyacrylate microspheres as fillers. The rigid skeleton of the modified calcium titanate whiskers and the flexible buffer of the modified magnesium borate whiskers form a stress gradient transmission, and the addition of polyacrylate microspheres can reduce local stress concentration. The three act synergistically to improve tensile strength and folding endurance. The high retention rate of acorn starch ensures that the filler is in full contact with the fiber, providing dense reaction sites for trans-aconitic acid crosslinking, thereby forming a water-resistant crosslinked network. Thus, the raw materials work synergistically to ensure that the produced specialty paper has excellent dry tensile strength, bursting resistance, folding endurance and wet strength retention rate.

The invention discloses a method for screening an anti-monochamus alternatus clonal pinus massoniana key gene based on genomics as well as an obtained gene and application thereof, and relates to the technical field of plant genetic engineering. The invention discloses a genomics-based method for screening an anti-monochamus alternatus clonal pinus massoniana key gene. The method comprises the following steps: screening a pinus massoniana anti-monochamus alternatus differential gene through a transcriptome; the masson pine anti-monochamus alternatus differential metabolites are screened out through the metabolome; according to a metabolome differential metabolite analysis result and in combination with a transcriptome differential gene analysis result, joint analysis is performed to construct an interaction network diagram, the pinus massoniana anti-monochamus alternatus key gene PmFLS is screened out, an overexpression strain of the pinus massoniana anti-monochamus alternatus key gene PmFLS is constructed, and the pinus massoniana anti-monochamus alternatus key gene PmFLS is obtained. Finally, a transgenic arabidopsis thaliana transgenic line with increased leaf area, advanced bolting time, increased total flavone content and increased FLS enzyme activity is obtained, and powerful support is provided for gene improvement of masson pine.

The invention relates to an ecological restoration composite structure for an acid rock cliff side slope. The ecological restoration composite structure is composed of a side slope stability strengthening system, a soil dressing layer anti-scouring stabilizing system, an acid-resistant plant community restoration system, a high-position water supply maintenance system and a water intercepting and draining ditch. The slope stability strengthening system forms a composite anchoring structure through full-length viscous anchor rods and a flexible protective net, and the soil dressing layer anti-scouring stabilizing system forms a multi-layer composite structure through a sprayed aggregate soil layer, ecological rods, a galvanized chain link net, an ecological base material layer, a reinforced microphone pad and anchoring parts. According to the acid-resistant plant community restoration system, acid-resistant indigenous plants such as masson pine with combined deep roots and shallow roots are sown in the soil dressing layer; the high-position water supply maintenance system provides maintenance water for the slope through a high-position water storage pool, a plastic hose, a PE water conveying pipe and a spraying device. According to the method, the problems of instability, scouring, difficulty in vegetation survival and the like of the acid rock cliff slope are systematically solved, and the long-term goals of slope stability and ecological restoration are achieved.

The present invention belongs to the field of plant culture technology, and in particular to a method for inducing the growth of adventitious roots from the terminal buds of a Masson pine adult tree, the method comprising: (1) generating axillary buds by terminal bud induction culture; (2) generating adventitious roots by axillary bud induction culture; wherein, in step (1), a first culture medium is used for cultivation, and the first culture medium is a DCR culture medium containing 6-benzylaminopurine. The method does not need to be rejuvenated, saves steps and time compared with existing adult tree regeneration methods, and inducing axillary buds and breeding from the terminal buds, the bud induction rate and the single bud induction rate are high, and the growth is good; in addition, the adventitious root hairs obtained by the method are numerous, and the roots are thick and strong.

The invention discloses a rhododendron lapponicum seedling culture medium and a preparation method thereof, and relates to the technical field of ornamental plant culture. The rhododendron lapponicum seedling culture medium is prepared from the following raw material components in parts by mass: 30 to 40 parts of masson pine needle compost, 25 to 35 parts of camellia oleifera shell ferment, 10 to 15 parts of perlite, 8 to 12 parts of vermiculite, 5 to 10 parts of peat soil, 0.3 to 0.8 part of folium cortex eucommiae extract, 0.2 to 0.6 part of honeysuckle vine extract, 0.5 to 1.0 part of humic acid and 0.3 to 0.7 part of calcium superphosphate. According to the method, agricultural and forestry waste source organic materials and inorganic conditioning materials are reasonably proportioned, plant extracts with biological activity are introduced, a seedling raising system which is synergistically optimized in the aspects of a physical structure, a chemical environment and biological functions is constructed, the growth vigor of seedlings is remarkably improved, and the yield of the seedlings is increased. A new technical scheme is provided for green, efficient and sustainable industrial seedling culture of the alpine rhododendron.

The invention discloses chaetomium formosanum and application thereof in prevention and treatment of pine wood nematode disease, and belongs to the technical field of biocontrol of plant diseases. The name of the bacterial strain is Chaetomium ascotrichioides 1-24-2, and the preservation number of the bacterial strain is GDMCC No: 64397. The invention further discloses a preparation method of the chaetomium ascotrichioides strain. The chaetomium formosanum 1-24-2 disclosed by the invention is derived from resistant masson pine plant tissues; the chaetomium formosanum 1-24-2 and the biological agent obtained based on the chaetomium formosanum 1-24-2 have a strong inhibition effect on pine wood nematode diseases, are low in requirements on culture conditions and have good development and application prospects.

This invention discloses a management method for improving the carbon sequestration capacity of Masson pine forests, belonging to the field of Masson pine forest management technology. The method includes steps such as land preparation and clearing, density optimization, mixed planting, formation of multi-layered forests, and post-management. Through the above method, pure Masson pine forests are transformed and managed into multi-layered mixed forests. During this process, fermented fertilizer prepared from pine needle litter is applied to increase the organic matter content of the forest soil, promote good tree growth, increase biomass, and optimize forest stand structure. This, in turn, enhances the carbon sequestration capacity of the tree layer, understory vegetation layer, and soil layer, comprehensively improving the carbon sink capacity of Masson pine forests, which is of great significance for improving the ecological environment.

The invention discloses application of a Pinus massoniana PmVNS2 gene, and belongs to the technical field of plant genetic engineering. The pinus massoniana PmVNS2 gene is obtained through cloning, the nucleotide sequence of the pinus massoniana PmVNS2 gene is shown as SEQ ID NO.1, and the expression protein sequence of the pinus massoniana PmVNS2 gene is shown as SEQ ID NO.2. A plant expression vector 35S:: PmVNS2 is constructed and transferred into a poplar leaf, and a poplar PmVNS2 transgenic plant is obtained through cultivation and screening. Compared with a control plant, the root number, plant height or ground diameter are obviously improved. Real-time fluorescent quantitative PCR finds that PmVNS2 significantly promotes up-regulation of expression levels of most secondary wall synthesis key enzyme genes and transcription factor coding genes in poplar; further scanning electron microscope results show that the number of heterologous expression transgenic poplar secondary xylem cell layers is obviously increased, and cell walls are obviously thickened. The invention provides an effective molecular tool and method for improving the properties of the poplar, and has a wide application prospect.

The invention belongs to the technical field of preparation of specialty paper, and particularly relates to a production method of specialty paper. According to the production method of the specialty paper, the bleached sulfate masson pine pulp and the bleached wheat straw pulp are compounded for use, a long-short fiber interlaced three-dimensional skeleton structure is constructed, modified calcium titanate whiskers, modified magnesium borate whiskers and polyacrylate microspheres are used as fillers, and the special paper is prepared by compounding the modified calcium titanate whiskers, the modified magnesium borate whiskers and the polyacrylate microspheres. The rigid framework of the modified calcium titanate whisker and the flexible buffering of the modified magnesium borate whisker form stress gradient transfer, the addition of the polyacrylate microspheres can reduce local stress concentration, the synergistic effect of the three can improve the tensile strength and folding strength, and the high retention rate of the acorn starch can ensure that the filler is in full contact with fibers. A compact reaction site is provided for trans-aconitic acid cross-linking, so that a water-resistant cross-linked network is formed. Therefore, through the synergistic effect of the raw materials, it is ensured that the produced specialty paper has excellent dry tensile strength, bursting strength, folding strength and wet strength retention rate.

Provided are robotic systems and methods for identifying a particular type of tree or plant (e.g., slash pine trees), for tapping them and collect their oleoresin for processing using an autonomous, long-range robotic forest rover. One aspect of the system comprises an autonomous vehicle equipped with an industrial robot, automated tool changer, a plurality of tools required for robotic operations, vision and navigation systems and powertrain for long-range operation. The rover identifies healthy, mature trees or plants, approaches them and performs the robotic operations required for tapping the trees or plants. In addition to these tasks, the Global Navigation Satellite System (GNSS) coordinates of the tree or plant is recorded along with its diameter, and a digital image of the tree or plant. This information is communicated to a base station for creating and updating a tree farm database.

The invention relates to the technical field of plant detection, in particular to application of a PmACAA1 gene in determination of masson pine seed vigor. The activity of the pinus massoniana seeds is judged by testing the relative expression quantity of the PmACAA1 gene, and if the expression quantity of acetyl coenzyme A acyltransferase is reduced, the activity of the pinus massoniana seeds is high. By means of the PmACAA1 gene, the activity of the pinus massoniana seeds can be efficiently and rapidly judged, and the method is not limited by the germination period of the seeds. According to the method provided by the invention, the efficiency of testing the activity of the pinus massoniana seeds is greatly improved, a good early-stage tool is provided for storage, later-stage cultivation and the like of the pinus massoniana seeds, and the economic benefit and the social benefit are good.

The invention belongs to the field of food, and relates to a solid beverage containing pine pollen and rosa sterilis extract and application of the solid beverage. The invention develops a compound solid beverage containing pine pollen and a rosa sterilis extract, overcomes the defects of poor stability, easy sedimentation and the like of a liquid beverage, and is specifically prepared from a masson pine pollen concentrated extract, a rosa sterilis concentrated extract, a sweetening agent, an adhesive, a preservative and a filling agent. Experiments prove that the solid beverage containing the pine pollen and the rosa sterilis extract has an anti-oxidation effect.

The present invention relates to the field of natural product separation technology, and more specifically, to a flavonoid compound isolated from Masson pine cones, a preparation method, and uses thereof. The preparation method of the flavonoid compound comprises alcohol extraction of the Masson pine cones, macroporous resin adsorption, and column separation. The compound disclosed in the present invention can be used for anti-inflammatory purposes, as well as in the preparation of RAW 264.7 cell inhibitors and in the preparation of TNF-α, IL-1β, and IL-6 protein expression inhibitors.

The invention relates to the field of insecticide preparation, and particularly discloses an insecticide taking mesoporous silica as a carrier and a preparation method of the insecticide. The abamectin sustained-release tablet comprises the following raw materials in parts by weight: 1-1.5% of mesoporous silica-loaded abamectin, 2.6-3.2% of fiber-loaded bacillus thuringiensis, 2-5% of a dispersing agent, 2-5% of a wetting agent, 1-2% of a binder and the balance of filler, wherein the total percentage is 100%. The preparation method comprises the following steps: S1, weighing the mesoporous silica carrier abamectin, the fiber carrier bacillus thuringiensis and the filler, and uniformly mixing and stirring to obtain a primary mixture; s2, adding a dispersing agent, a wetting agent and a binder into the primary mixture, and uniformly mixing and stirring to obtain an insecticide; the pesticide has the advantage of efficiently killing the pine caterpillars on the masson pine.

Provided are robotic systems and methods for identifying a particular type of tree or plant (e.g., slash pine trees), for tapping them and collect their oleoresin for processing using an autonomous, long-range robotic forest rover. One aspect of the system comprises an autonomous vehicle equipped with an industrial robot, automated tool changer, a plurality of tools required for robotic operations, vision and navigation systems and powertrain for long-range operation. The rover identifies healthy, mature trees or plants, approaches them and performs the robotic operations required for tapping the trees or plants. In addition to these tasks, the Global Navigation Satellite System (GNSS) coordinates of the tree or plant is recorded along with its diameter, and a digital image of the tree or plant. This information is communicated to a base station for creating and updating a tree farm database.

The present invention discloses a non-destructive measurement method for the morphological indexes of Masson pine seedlings, comprising: collecting and correcting Masson pine images; reconstructing the collected images into 3D point clouds and pre-processing the point clouds; creating a dataset for semantic segmentation of the Masson pine point clouds, calculating class balance weights for different classes in the dataset; constructing a densely weighted semantic segmentation network model; assigning the class balance weights to the loss function of the model; training the densely weighted semantic segmentation network model using the dataset; obtaining the original skeleton points of the stem based on the points of the Masson pine stem in the Masson pine point cloud segmented by the densely weighted semantic segmentation network model, and refining and extracting the skeleton points of the stem; and extracting the morphological index values of the Masson pine to be measured based on the refined skeleton points of the stem. The present invention can accurately measure the morphological indexes of Masson pine seedlings, no longer relying on manual labor and specialized instrument measurement equipment, and reducing measurement costs and difficulty.

The invention discloses a pinus massoniana gene silencing method, which is based on a plant virus vector and can effectively realize pinus massoniana gene silencing through an optimized transduction method. Experimental results based on a phytoene dehydrogenase gene (PmPDS) show that the silencing efficiency of the method can reach 79.1%; experimental results based on a plant geranyl geranyl pyrophosphate synthase 6 (PmGGPPS6) and an NAC transcription factor family 5 gene (PmNAC5) show that the method has wide applicability and can be used for silencing different types of genes. According to the method, the application range in the field of pinus massoniana gene function research is widened, possibility is provided for deep research of gene functions in multiple aspects such as pinus massoniana growth and development and metabolism regulation, and a new thought and method are provided for genetic improvement and variety cultivation of pinus massoniana; the cultivation of pinus massoniana varieties with specific phenotypes or excellent characters is facilitated, and the economic value and ecological benefits of the pinus massoniana varieties are improved.

The invention discloses a method for interplanting phoebe zhennan in a pure masson pine forest to cultivate a mixed forest, and belongs to the technical field of masson pine forest management. Large-size phoebe zhennan container seedlings are cultivated and transplanted, a root cluster dispersing agent is prepared and applied during transplanting, the dispersing agent can effectively disperse root clusters and promote root systems to extend into soil, and therefore the soil quality is improved; the method solves the problem that the growth condition is reduced due to limited nutrient and moisture absorption after transplanting caused by root system winding and seedling raising container obstruction of large-sized nanmu container seedlings, ensures the survival rate and good growth condition of the large-sized nanmu container seedlings cultivated in the masson pine forest, and can effectively improve the management benefit of the masson pine forest. The method disclosed by the invention has a good application prospect in operation and transformation of the pure masson pine forest.

The invention provides a sweet-scented osmanthus composition with the effects of resisting depression, relieving anxiety and regulating emotion as well as a preparation method and application of the sweet-scented osmanthus composition, and relates to the field of natural plant medicines. The sweet-scented osmanthus composition with the effects of resisting depression, relieving anxiety and regulating emotion comprises the following raw materials: a sweet-scented osmanthus petal extract, a sweet-scented osmanthus leaf extract and a sweet-scented osmanthus root extract, the mass ratio of the sweet-scented osmanthus petal extract to the sweet-scented osmanthus leaf extract to the sweet-scented osmanthus root extract is (40-60): (25-35): (15-20). Furthermore, the composition also contains a masson pine extract and patrinia villosa juss leaf powder, and the mass ratio of the osmanthus flower petal extract to the osmanthus flower leaf extract to the osmanthus flower root extract to the patrinia villosa juss leaf extract to the masson pine extract is (40-60): (25-35): (15-20): (8-10): (3-5). The sweet-scented osmanthus composition developed by the invention not only has a good anti-depression effect, but also has a good anti-anxiety effect, has double effects in the aspect of emotion regulation, and can give full play to the industrial value of sweet-scented osmanthus.

The present invention discloses a strain of Trichoderma paraviridescens and its application in controlling plant diseases, belonging to the technical field of plant disease control. The preservation number of the Trichoderma paraviridescens is CGMCC No. 41126. The present invention also discloses the application of the Trichoderma paraviridescens or a broad-spectrum antibacterial agent containing the Trichoderma paraviridescens in inhibiting plant pathogenic bacteria. The Trichoderma paraviridescens provided by the present invention has a significant antagonistic effect against Diplodia pinea, the pathogen causing dead twig disease of Masson pine, and other common plant pathogenic bacteria, is environmentally friendly and pollution-free, provides a safe and reliable new way for the control of various plant diseases, and has important significance in the field of biological control of plant diseases. Moreover, the Trichoderma paraviridescens provided by the present invention is simple to culture, easy to preserve, has a wide antibacterial spectrum, is convenient for industrial production, and has good development and application prospects.

The application discloses a kind of packing paper preparation method, comprising the following steps: 1) surface embryo layer preparation: the bleaching treatment of mixed pulp is obtained by mixing camphor tree wood pulp, masson pine wood pulp and waste paper pulp, after being sequentially coated, pressed, dried, coiled and polished, surface embryo layer is obtained;2) impregnation hot-pressing treatment: surface embryo layer is prepared for use by impregnation hot-pressing treatment with resin;3) inner embryo layer preparation: the bleaching treatment of mixed pulp is obtained by mixing camphor tree wood pulp, masson pine wood pulp and waste paper pulp, after being sequentially coated, pressed, dried, coiled and polished, inner embryo layer is obtained;Before coating operation, antibacterial agent is added to mixed pulp;4) hot-rolled composite treatment: the inner embryo layer and the surface embryo layer after impregnation hot-pressing treatment are arranged with reinforcing layer, the reinforcing layer is fiber mesh layer, and hot-rolled bonding can be achieved.The application can prepare composite packing paper with good waterproofness, good strength and good antibacterial property, and the composite packing paper is suitable for preparing packaging bag of pre-packaged food.

The invention discloses a method for imitating wild cultivation of dictyophora rubrovolvata in a pinus massoniana and gleditsia sinensis mixed forest land. The method comprises the following steps: selecting and cleaning the pinus massoniana and gleditsia sinensis mixed forest land, removing bags from dictyophora rubrovolvata clinker mushroom sticks suitable for being planted under the forest, placing furrows, covering rich bacterial fertilizer humus soil, and carrying out imitating wild cultivation under the protection of a rain-sheltering small arched shed and a sunshade net. Aiming at different growth stages of the under-forest dictyophora indusiata, the culture environment is regulated and controlled in time, the nutrient solution and the microbial liquid fungicide in each stage are creatively applied, the hypha soil climbing time is shortened by 3 days, the mushroom egg culture time is shortened by 7 days or above, the incidence rate of dictyophora rubrovolvata diseases and pests is reduced by 10% or above, and the method has the advantages that the biotransformation rate of the mushroom sticks is increased by 5%, and the yield of the woodland dictyophora rubrovolvata is increased. According to the method, the under-forest vacant land is developed and utilized, a greenhouse does not need to be built or soil in the greenhouse does not need to be replaced every year, the soil continuous cropping obstacle problem of bamboo fungus planting is solved, and the cost for building the greenhouse and replacing the soil per mu is saved by more than 10,000 yuan; the method is easy to popularize, and can promote the development of the under-forest bamboo fungus planting industry and under-forest economy.

The invention discloses a carbon sequestration concrete paving material suitable for a high-humidity environment and a preparation method of the carbon sequestration concrete paving material, and belongs to the technical field of carbon sequestration concrete.The paving material is prepared from, by mass, pretreated masson pine epidemic wood chippings, plant chippings, magnesium chloride, magnesium silicate, sodium silicate, stearic acid and a coloring agent, the paving material is prepared from the following raw materials: pretreated masson pine epidemic wood chips, plant chips, magnesium chloride, magnesium silicate, sodium silicate, stearic acid and a coloring agent in a mass ratio of (30-35): (20-25): (5-10): (10-15): (10-12): (6-8): (0-2). The paving material solves the problems that the existing carbon-sequestration concrete paving material prepared from masson pine epidemic wood is insufficient in strength, easy to crack and easy to rot in a high-humidity region.

The invention belongs to the field of forestry carbon neutralization, and discloses a carbon emission accounting and carbon footprint evaluation method in a masson pine operation process. According to the method, based on a full life cycle dynamic evaluation principle of a masson pine operation process and product production, the masson pine operation and product production process is divided into two sub-stages: a forestation stage and a product production stage; the method comprises the following steps: tracking production activity input and output of process units in each stage, constructing a life cycle list, constructing a carbon footprint accounting model of masson pine operation and product production processes by using an emission factor method according to different activities and carbon emission types, and meanwhile, carrying out uncertainty and sensitivity analysis to realize scientific carbon footprint accounting. The technical gap of carbon footprint accounting of the whole life cycle of the timber forest product is filled, the transparency and accuracy of the whole industry chain accounting of the forestry product are improved, the process low-carbon regulation and production technology improvement are facilitated, the cost is further reduced, the supply chain is comprehensively optimized, and the overall carbon footprint is reduced.

The invention discloses a carbon sequestration concrete material based on masson pine epidemic wood and a preparation method thereof, and belongs to the technical field of carbon sequestration concretes.The carbon sequestration concrete material is prepared from masson pine epidemic wood, bamboo fibers, magnesium oxide, magnesium chloride, fly ash, xylinic acid, disodium hydrogen phosphate, sodium lauroyl isethionate and octadecyl dimethyl betaine; pinus massoniana epidemic wood is crushed and then mixed with other raw materials, then magnesium oxide and magnesium chloride are adopted as adhesives to prepare the carbon sequestration concrete material, high-quality utilization of the Pinus massoniana epidemic wood resources is greatly improved, and the carbon sequestration concrete material prepared according to the method is high in strength, good in efflorescence and scumming resistance, not prone to cracking and suitable for large-scale production. Good application prospects are realized.

The present invention provides a method and device for replanting and repairing degraded masson pine forests, belonging to the technical field of forest resource management. A method for replanting and repairing degraded masson pine forests according to the present invention establishes a forest resource map, details regional resource information and tree grades, directly screens each logging area in stages based on the regional resource information and tree grades, rather than only considering the infected area, can more reasonably and gradually determine the target logging area, and in combination with the forest resource map, accurately determine the replanting area, maximize the utilization of the vacant land resources after logging, accelerate the regeneration of forest resources, improve the utilization efficiency of forest land resources, ensure the rationality of the logging area and distance, and rationally centralize the distribution of the logging and replanting areas, which can reduce the logging and replanting frequency, difficulty and cost, and improve the operation efficiency.

The invention relates to a near-natural transformation method for a masson pine man-made forest. The near-natural transformation method comprises the following steps: (1) selecting and cultivating target trees, resin trees and other trees in a middle-age masson pine man-made forest, and growing interference trees and pitaya trees; (2) cultivating and cutting off resin trees; complementary planting of broad-leaved tree species and tending are carried out, and a different-age pine and broad-leaved mixed forest is obtained; and (3) selecting and marking second-generation target trees in the broad-leaved tree species subjected to complementary planting, selectively cutting interference trees and cultivating the second-generation target trees, then selectively cutting mature masson pine target trees and the second-generation target trees in sequence, and then continuously using the target tree single-plant operation method for operation to complete near-natural transformation. According to the transformation method, masson pine large-diameter wood cultivation and rosin production are both considered, tree species composition and space structure are improved, biodiversity is enriched, forest health is maintained, multiple functions of the forest are fully played, and healthy, stable, high-quality and efficient close-to-nature man-made forests are cultivated.

The invention discloses a method and system for estimating the total carbon reserve of a target ecological system carbon library, and relates to the technical field of forestry information, and the method comprises the following steps: carrying out the sample collection of a target ecological system; the vegetation type of the target ecological system is a subtropical evergreen broad-leaved forest, cypress, masson pine, alder and quercus acutissima are taken as main materials, the altitude is 182-954 m, the gradient is 0-45 degrees, the soil is yellow soil and purple soil, and the thickness of a dry branch and fallen leaf layer is 0-15 cm; the sample comprises at least one of a soil sample, a vegetation sample and a litter layer sample; carrying out analysis and calculation on the basis of the sample to obtain the total carbon reserve and carbon density of the target ecological system; the total carbon reserves and carbon density distribution characteristics of the target ecological system comprise the carbon density of a vegetation layer, the carbon reserves of the vegetation layer, the carbon density of a soil layer, the carbon reserves of the soil layer, the carbon density of a litter layer and the carbon reserves of the litter layer.