36 results about "Trichoderma virens" patented technology

The invention relates to the field of fertilizers, in particular to a stable fertilizer for preventing and treating peanut root rot and a preparation method thereof, and the fertilizer comprises a fertilizer inner core layer, a nano microbial metabolite layer and a microbial protective layer from inside to outside. According to the stable fertilizer for preventing and treating peanut rhizome rot, the capsule embedding inhibitor made of humic acid and the trichoderma aureoviride metabolite are adopted to construct a dual nitrification inhibition system, and the capsule embedding inhibitor and the trichoderma aureoviride metabolite have a synergistic effect, so that the nitrification effect is reduced, the nitrogen fertilizer utilization rate is increased, and non-point source pollution is reduced.

The invention relates to the technical field of biological prevention and control, in particular to a trichoderma subatroviride Thp30 strain and application thereof. The trichoderma subatroviride Thp30 is preserved in Guangdong Microbial Culture Collection Center on May 22, 2025, and the preservation number is GDMCC No: 66381. The trichoderma pseudoviride Thp30 provided by the invention can inhibit the growth of wheat stem rot pathogen fusarium graminearum, apple anthracnose pathogen colletotrichum gloeosporioides, botrytis cinerea pathogen botrytis cinerea, wax gourd fusarium wilt pathogen fusarium oxysporum and pear anthracnose pathogen colletotrichum gloeosporioides, and promote the growth of wheat, soybean and wax gourd; the wheat basal stem rot can be effectively prevented and treated, and the freezing resistance of wheat can be enhanced.

The invention relates to the field of microorganisms, and discloses a complex microbial inoculant for preventing and treating clubroot of rape. Comprising the following raw materials in parts by weight: 10 to 12 parts of acid-resistant bacillus, 12 to 15 parts of pseudomonas fluorescens PF-5, 8 to 10 parts of bacillus mucilaginosus, 5 to 7 parts of halophilic bacillus, 8 to 10 parts of bacillus amyloliquefaciens, 12 to 15 parts of trichoderma atroviride ReTv2, 6 to 8 parts of azotobacter chroococcum, 10 to 12 parts of chitosan-gamma-polyglutamic acid microspheres, 12 to 15 parts of diatomite and 8 to 12 parts of humic acid. And 2-4 parts of algal polysaccharides. The living space of plasmodiophora brassicae is preempted by the acid-resistant bacillus; the pseudomonas fluorescens PF-5 directly strikes pathogenic bacteria and stimulates the defense system of the oilseed rape; the bacillus amyloliquefaciens releases a plurality of antibacterial substances; the trichoderma atroviride ReTv2 rapidly destroys the structure of the plasmodiophora brassicae in a manner of hyperparasitism and exoenzyme secretion.

The invention relates to a trichoderma virens strain and application thereof. According to the invention, a Trichoderma virens strain GZUAS831026 is separated from a capsicum land soil sample, the Trichoderma virens strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the Trichoderma virens strain is GDMCC No: 65370. The trichoderma virens strain obtained by the invention has a good pepper straw degradation effect and a growth promoting effect, has a good inhibition effect on pathogenic bacteria of the ginger tuber rot disease, and can be used for preventing and treating the ginger tuber rot disease.

The invention discloses a trichoderma virens preparation capable of preventing diseases and increasing yield as well as a preparation method and application of the trichoderma virens preparation. Belongs to the technical field of biological agents. According to the trichoderma virens strain T259 disclosed by the invention, the trichoderma virens strain T259 is respectively subjected to strain amplification culture, liquid amplification culture and solid fermentation culture, a solid fermentation product of the T259 strain is successfully prepared, the solid fermentation product is mixed with an auxiliary agent, and the trichoderma virens preparation capable of preventing diseases and increasing yield is prepared. The trichoderma virens spore content in the preparation is improved; solid fermentation can effectively improve the colonization ability of trichoderma virens in the field. The production of the trichoderma virens preparation has the characteristics of large sporulation quantity, short period, wide raw material source and low cost; the fertilizer is easy to use and operate, suitable for various crops and remarkable in disease prevention and yield increase effect. And a foundation is laid for quality and efficiency improvement and high-quality and high-efficiency development of modern agriculture.

The invention provides application of trichoderma evergreen in relieving continuous cropping obstacles of ginseng or salvia miltiorrhiza, and relates to the technical field of crop cultivation. Through deep research and creative labor, the trichoderma evergreen Tri403 can effectively relieve soil chemical property deterioration and soil microorganism change after continuous cropping of the ginseng or the salvia miltiorrhiza, further can improve the soil chemical property after continuous cropping of the ginseng or the salvia miltiorrhiza, increases the relative abundance of beneficial bacteria in the soil, inhibits the relative abundance of harmful bacteria, and improves the yield of the ginseng or the salvia miltiorrhiza. Meanwhile, the content of effective components of ginseng or salvia miltiorrhiza can be increased, and the quality of continuous cropping ginseng or salvia miltiorrhiza is improved.

The invention discloses application of trichoderma xerophyllum to prevention and treatment of plant root-knot nematodes, and belongs to the technical field of microorganisms, the trichoderma xerophyllum is preserved in Guangdong Microbial Culture Collection Center, the number is GDMCC No: 66977, and the preservation address is No.100, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province. The preparation method of the fungicide prepared based on the trichoderma xerophyllum comprises the following steps: transferring the strain into a seed culture medium, and culturing on a shaking table at the temperature of 25-30 DEG C and the speed of 150-200r / min for 24-36 hours to obtain a fermentation seed solution; inoculating the fermentation seed solution into a fermentation culture medium, and culturing for 72-96 hours on a shaking table at the temperature of 25-30 DEG C and the speed of 150-200 r / min to obtain a fermentation solution, namely the target fungicide. The trichoderma xerophyllum has a remarkable lethal effect on root-knot nematodes, the fatality rate of fermentation liquor of the trichoderma xerophyllum on the root-knot nematodes exceeds 90%, the fatality rate of 80% or above can still be kept even if the trichoderma xerophyllum is diluted by 50 times, the trichoderma xerophyllum acts quickly, nematodes can be quickly killed within 12 hours, larvae are effectively prevented from invading plants, and the trichoderma xerophyllum shows a good application prospect in the field of prevention and treatment of the plant root-knot nematodes.

The invention discloses a microbial acidic soil conditioner, and belongs to the technical field of soil improvement. The microbial acid conditioner comprises trichoderma virens T259 with the preservation number of CGMCC No.41109. Through the synergistic interaction of the trichoderma virens T259, the calcium magnesium phosphate fertilizer, the organic fertilizer, the fulvic acid and the chitosan, the acid-base property of soil can be adjusted, and the microbial structure of the soil can be improved; the composition can also induce crops to generate resistance and promote crop growth, so that resistance and development to diseases are enhanced, and the yield and quality are improved.

This invention provides a *Trichoderma viride* RM9 strain antagonizing jasmine white mold disease and its application. The *Trichoderma viride* RM9 strain of this invention is protected under the accession number CCTCC M 20221442. *Trichoderma viride* RM9 of this invention is isolated from the rhizosphere of jasmine plants, exhibits strong rhizosphere colonization ability, and demonstrates good control efficacy against jasmine white mold disease, achieving a control effect of 83.34%. It also promotes the growth of jasmine seedlings. Furthermore, its cultivation conditions are relatively simple, and its development into a solid inoculant is beneficial for improving jasmine yield and economic benefits.

The present invention belongs to the technical field of crop control, and discloses a compound microbial agent containing Bacillus pumilus and Trichoderma viride and its application in biological control of citrus. The Bacillus pumilus is Bacillus pumilus ( Bacillus pumilus ), HBTG2356, and its preservation number is: CCTCC NO: M20232119. The Trichoderma viride is Trichoderma viride ( Trichoderma virens ), HBTG3206, and its preservation number is: CCTCC NO: M20232117. Compared with single strains, this compound strain has a synergistic effect on diseases such as citrus canker and citrus root-knot nematode, and has a certain application prospect in the prevention and control of citrus diseases.

The invention relates to a method for separating and purifying active substances in trichoderma citrinoviride fermentation liquor, in particular to a method for separating and purifying trichoderma citrinoviride fermentation liquor. Comprising the steps of extraction of trichoderma citrinoviride fermentation liquor and purification and analysis of active substances in the trichoderma citrinoviride fermentation liquor, the trichoderma citrinoviride fermentation liquor is subjected to ethyl acetate extraction and vacuum concentration to obtain a crude extract, an efficient preparative liquid phase is adopted for separation and purification in the purification and analysis step, and methanol and formic acid water are selected in the purification and analysis step. Through the steps of extracting the trichoderma citrinoviride fermentation liquor and purifying and analyzing the active substances in the trichoderma citrinoviride fermentation liquor, the separation and purification process of the trichoderma citrinoviride fermentation liquor is simple, the cost is low, the operation is simple and convenient, and the product purity is high.

The invention discloses a compound microorganism for preventing and treating chestnut fruit rot and application of the compound microorganism. The compound microorganism is composed of trichoderma atroviride, bacillus siamensis and bacillus velezensis, and is constructed by directionally screening strains with broad-spectrum inhibition effect on seven core pathogenic bacteria from Chinese chestnut fruit endophytes and verifying compatibility. The method has the advantages that the compound flora synergistically prevents and controls diseases through direct antagonism and induction of plant system resistance, and the defensive enzyme activity and the content of total polyphenol and total flavone of Chinese chestnuts are remarkably improved; according to the compound microorganism, the indoor control effect reaches 90% (the morbidity is reduced), the diseased fruit rates of Chinese chestnut fruits treated in a chestnut garden are reduced by 60% and 42.86% respectively after the Chinese chestnut fruits are stored for 30 days and 120 days, the effectiveness and the stability are obviously higher than those of a single strain, the compound microorganism can be introduced in the flowering period or sprayed in the storage period, use is convenient and flexible, and an efficient and stable biocontrol strategy is provided for green prevention and control of the Chinese chestnut fruit rot.

The invention relates to the technical field of planting, in particular to a fungicide and fertilizer suitable for planting landscape plants. The microbial inoculum disclosed by the invention is prepared from the following components in parts by mass: 5 to 10 parts of bacillus coagulans microbial inoculum, 4 to 8 parts of bacillus laterosporus microbial inoculum, 10 to 18 parts of bacillus mucilaginosus microbial inoculum, 6 to 10 parts of bacillus velezensis microbial inoculum, 2 to 6 parts of trichoderma viride, 6 to 10 parts of bacillus amyloliquefaciens microbial inoculum and 2 to 6 parts of paecilomyces lilacinus. Through compatibility of various microbial agents, the soil structure can be improved, and the soil fertility can be improved. The microbial agent disclosed by the invention is prepared into a fertilizer, and the obtained fertilizer also has the effects of regulating the soil microhabitat, improving the soil structure and improving the soil fertility. The growth of garden plant roots can be promoted, and the growth performance of garden plants is improved. And a basis is provided for planting of landscape plants.

The invention provides a crop nutritional supplement containing glycopeptide as well as a preparation method and application of the crop nutritional supplement, and belongs to the technical field of biological agriculture. The crop nutritional supplement comprises fish protein peptide, fucose oligosaccharide, fulvic acid, silicon-calcium-potassium-magnesium fertilizer, bone meal, polyglutamic acid, a microbial agent and a carrier. Wherein the fish protein peptide is obtained by fermenting deep-sea fish meat with bacillus subtilis SH21 and performing enzymolysis with compound enzyme, and the microbial agent is prepared from trichoderma atroviride C61 and paenibacillus mucilaginosus HB-02. The crop nutritional supplement can activate the immune system of crops, enhance disease resistance, promote nutrient absorption and improve the yield and quality of crops, is environment-friendly, and can solve the problems caused by chemical pesticides and traditional chemical fertilizers.

The invention discloses Trichoderma virens (Trichoderma virens) and an application of the Trichoderma virens (Trichoderma virens). The invention provides a Trichoderma virens strain with strong hyperparasitism on peanut sclerotium rolfsii and sclerotinia sclerotiorum, the Trichoderma virens strain is named as MJ18, is preserved in China Center for Type Culture Collection (CCTCC) on January 15, 2025, and has a preservation number of CCTCC NO: M 2025149. The trichoderma virens MJ18 can be used for inhibiting peanut sclerotium rolfsii and sclerotinia sclerotiorum, can also be used for inhibiting plant pathogenic fungi such as pythium ultimum, fusarium graminearum, fusarium pseudograminearum and rhizoctonia solani, and can be used for preventing and treating related plant diseases caused by the pathogenic fungi. Besides, the trichoderma virens MJ18 can improve the emergence rate of the peanuts and promote the growth of the peanuts in the seedling stage, can be combined with chemical insecticides for use, and can be used as a seed coating agent to promote the germination of the peanuts and realize the common prevention and control of the southern blight of the peanuts and the insect pests of the peanuts at the same time.

The invention provides application of trichoderma atroviride in relieving continuous cropping obstacles of ginseng or salvia miltiorrhiza, and relates to the technical field of crop cultivation. Through deep research and creative labor, the trichoderma atroviride Tri802 can effectively relieve soil chemical property deterioration and soil microorganism change after continuous cropping of the ginseng or the salvia miltiorrhiza, further can improve the soil chemical property after continuous cropping of the ginseng or the salvia miltiorrhiza, increases the relative abundance of beneficial bacteria in the soil, inhibits the relative abundance of harmful bacteria, and improves the yield of the ginseng or the salvia miltiorrhiza. Meanwhile, the content of effective components of ginseng or salvia miltiorrhiza can be increased, and the quality of continuous cropping ginseng or salvia miltiorrhiza is improved.

A method for isolating and selecting Trichoderma Virens with an aerobic denitrification function and a method for remediating a water body with a low carbon-to-nitrogen ratio using the Trichoderma Virens are provided. Compared with the prior art, the biological treatment adopted by the present disclosure can allow the relatively-complete removal of nitrates without producing a residue, and exhibits advantages such as low cost, efficiency, and eco-friendliness. Further, the biological treatment adopted by the present disclosure can enhance the resistance of the fungus to toxic compounds and harsh environments, significantly promote the removal of nitrogen and organic matters in a water body, improve a decontamination ability of a water body, and increase the diversity of microorganisms in a water body, thereby accelerating the promotion of remediation of a water quality of a water body with a low carbon-to-nitrogen ratio. Therefore, the present disclosure has an extensive application potential.

The invention discloses a trichoderma atroviride engineering strain for expressing dsRNA (double-stranded ribonucleic acid) as well as a preparation method and application of the trichoderma atroviride engineering strain, the trichoderma atroviride engineering strain is trichoderma atroviride which is integrated with a dsRNA expression box expressed by an interference target gene BdSpt7, the dsRNA expression box is as shown in SEQ ID No.1, and the trichoderma atroviride is trichoderma atroviride T11 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41710. The preparation method of the trichoderma atroviride engineering strain comprises the following steps: constructing a dsRNA expression framework, constructing a recombinant expression vector and transforming protoplast. The trichoderma atroviride engineering strain can stably express a dsRNA expression cassette of a target gene BdSpt7 so as to play a role in preventing and treating physalospora piricola, is stable in performance and high in delivery efficiency, and can be used for preparing a biocontrol preparation and applied to prevention and treatment of physalospora piricola.

The application discloses a kind of for preventing and treating chestnut fruit rot disease complex microorganism and its application.The application complex microorganism is composed of Trichoderma atroviride and Bacillus siamensis, Bacillus velezensis, by from chestnut fruit endophyte directional screening to seven kinds of core pathogenic bacteria have broad-spectrum inhibitory effect strain, is constructed after compatibility verification.It is superior in that: complex microbial population is synergistically controlled disease by direct antagonism and induced plant system resistance, significantly improves chestnut defense enzyme activity and total polyphenol, total flavone content;Indoor control efficiency reaches 90% (reduces the incidence), chestnut fruit in chestnut orchard treatment is reduced 60% and 42.86% respectively in 30 days and 120 days, effective and stability are obviously stronger than single strain, and the application complex microorganism can be introduced by flowering period or sprayed in storage period, and is convenient and flexible, provides efficient, stable biocontrol strategy for chestnut fruit rot green control.

The invention belongs to the technical field of microorganisms, and relates to trichoderma atroviride SH-L1 and application thereof. The preservation number of the trichoderma atroviride SH-L1 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 41751. The invention also provides a microbial agent, which comprises trichoderma atroviride SH-L1 and / or fermentation liquor or metabolite of the trichoderma atroviride SH-L1. The trichoderma atroviride SH-L1 obtained by separation and screening can inhibit the growth of aspergillus flavus, inhibit the generation of aflatoxin or reduce the content of aflatoxin, promote the growth of peanut seedlings, improve the peanut yield and significantly inhibit peanut soil-borne bacteria, and has the advantages of being green, environmentally friendly, high in specificity, environmentally friendly and the like; peanut yield and quality safety can be guaranteed, and healthy development of the peanut industry is promoted.

The invention relates to the technical field of agricultural microbial preparations, and discloses a sweet potato root rot antagonistic fungus preparation as well as a preparation method and an application method thereof. The preparation consists of a trichoderma harzianum and trichoderma atroviride compound strain, a modified charcoal-humic acid organic carrier, a chitosan-sodium alginate microcapsule wall material, a dextrin-trehalose stabilizer and a sodium salicylate / methyl jasmonate synergistic factor, and is prepared by adopting a solid state fermentation process, a fluidized bed drying process, a microcapsule embedding process and a fluidized bed granulation process. The field control effect reaches 78%-92%, the lasting period is 3-4 months, and the pesticide composition has no inhibition effect on beneficial microorganisms in soil.

The present invention discloses a Trichoderma atroviride and its application, belonging to the field of biological control of plant diseases. The Trichoderma atroviride was deposited at the China Center for Type Culture Collection (CCTCC) on July 19, 2022, with the deposit number of CCTCC No. M 20221135. The Trichoderma atroviride CJCCSJ-F-KY10039 was isolated from the forest system in Xishuangbanna. The fermentation supernatant of this Trichoderma atroviride strain can inhibit the growth of the hyphae of Fusarium graminearum and Fusarium verticillioides. Through the plate confrontation test, it was found that its strain has an inhibitory effect on the growth of Fusarium verticillioides and Fusarium graminearum. This strain has good application prospects in the biological control of Fusarium graminearum and Fusarium verticillioides in maize.

This invention discloses a biocontrol compound microbial strain YC and its application, relating to the field of microbial technology. The biocontrol compound microbial strain YC is composed of *Trichoderma harzianum* YP2 and *Trichoderma viride* CK1A. *Trichoderma harzianum* YP2 was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and is classified as follows: Trichoderma afroharzianum YP2, with accession number CGMCC NO. 41947; the described *Trichoderma viride* CK1A was deposited on May 22, 2025, at the China General Microbiological Culture Collection Center, and classified as... Trichoderma atroviride CK1A, with accession number CGMCC NO. 41948. The biocontrol compound microbial system YC described herein is used to control maize stalk rot. The biocontrol compound microbial system YC of this invention exhibits stable efficacy and strong antibacterial ability, opening up new avenues for the biological control of maize stalk rot in my country.

The invention discloses a method for improving plant disease resistance and increasing fragrance. The method comprises the steps of soil preparation, wherein loose and fertile soil with high permeability is selected; illumination management: natural illumination is not less than 6 hours every day, and a plant ultraviolet light supplement LED lamp is adopted to supplement light for not less than 4 hours every day before flowering; according to the water requirement characteristic of the plants, the watering principle that the plants are dry and wet is adopted, and the watering time is selected in the morning or at night; temperature management: the environment temperature is maintained at 20-35 DEG C; special treatment: mixing potassium dihydrogen phosphate, phenylalanine, polyaspartic acid, cane sugar, L-glycine, cysteine, farnesyl pyrophosphoric acid, a pelargonium graveolens extract, trichoderma virens, trichoderma asperellum, bacillus subtilis, potassium sorbate, benzoic acid, jasmonic acid and vitamin C to obtain a composition; the composition is diluted with water by 500-600 times, and then the plant root zone is irrigated once every 7-10 days in the growth period of the plant. The method can improve plant disease resistance and increase flower plant fragrance.

The present invention discloses a strain of Trichoderma paraviridescens and its application in controlling plant diseases, belonging to the technical field of plant disease control. The preservation number of the Trichoderma paraviridescens is CGMCC No. 41126. The present invention also discloses the application of the Trichoderma paraviridescens or a broad-spectrum antibacterial agent containing the Trichoderma paraviridescens in inhibiting plant pathogenic bacteria. The Trichoderma paraviridescens provided by the present invention has a significant antagonistic effect against Diplodia pinea, the pathogen causing dead twig disease of Masson pine, and other common plant pathogenic bacteria, is environmentally friendly and pollution-free, provides a safe and reliable new way for the control of various plant diseases, and has important significance in the field of biological control of plant diseases. Moreover, the Trichoderma paraviridescens provided by the present invention is simple to culture, easy to preserve, has a wide antibacterial spectrum, is convenient for industrial production, and has good development and application prospects.

The invention relates to the technical field of microorganisms, and discloses trichoderma evergreen, a leavening agent containing the trichoderma evergreen, a preparation method and application of the leavening agent, and a method for preventing and treating hydroponic barley mould. The preservation number of the Trichoderma viridescens (Trichoderma viridescens) is CCTCC (China Center For Type Culture Collection) M 2025913. The leavening agent contains the trichoderma evergreen, the leavening agent acts on wheat seeds and wheat seedlings of hydroponic barley, growth of barley mould can be effectively inhibited, the leavening agent is safe, environmentally friendly, controllable in cost and suitable for large-scale application, and the urgent requirement of modern organic agriculture for the green prevention and control technology is met.

The invention belongs to the technical field of microorganisms, and discloses trichoderma atroviride, a biocontrol microbial agent and application of the trichoderma atroviride, the trichoderma atroviride is named as trichoderma atroviride T11, the preservation number of the trichoderma atroviride T11 is CGMCC No.41710, the trichoderma atroviride is preserved in China General Microbiological Culture Collection Center on December 11, 2024, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The active ingredient of the biocontrol inoculant is the trichoderma atroviride. The trichoderma atroviride T11 has an inhibiting effect on various plant disease pathogenic bacteria, also has a growth promoting effect on fruit trees, can be used for preparing a biocontrol microbial inoculum and applied to prevention and treatment of fruit and vegetable diseases, can also be used as an active ingredient for preparing a plant growth promoting agent, and is stable in effect and environment-friendly.

Compositions and methods for improving one or more aspects of plant growth using synergistically effective amounts of one or more lipo-chitooligosaccharides and one or more agriculturally beneficial microorganisms, such as Streptomyces lydicus WYEC 108, Trichoderma virens GI-3 and Bacillus amyloliquefaciens SB3778.

The present invention belongs to the field of fungal genetic breeding technology and specifically discloses a single-nucleotide polymorphism (SNP) molecular marker associated with the dark green Trichoderma resistance trait in Lentinus edodes and its application. The SNP molecular marker is located on chromosome 5 of the Lentinus edodes reference genome. At base 479,587, a C-to-T mutation occurs. Lentinus edodes with the T / T genotype exhibit high dark green Trichoderma resistance. This molecular marker can efficiently determine Trichoderma resistance during the mycelial stage of Lentinus edodes, overcoming the shortcomings of traditional breeding that relies on phenotypic identification. This accelerates the selection and breeding of new Trichoderma-resistant Lentinus edodes varieties and improves breeding efficiency.

The present application relates to the technical field of fertilizer preparation, and particularly relates to a compound microbial fertilizer for preventing root rot. The compound microbial fertilizer comprises 45-55 parts of compound fertilizer, 20-26 parts of wood ash, 6-10 parts of humic acid, 3-7 parts of diatomite, 4-8 parts of compound microbial agent and 8-12 parts of traditional Chinese medicine extract. The compound microbial agent is composed of bacillus licheniformis, bacillus cereus, bacillus atrophaeus, pseudomonas chlororaphis, pseudomonas brassicacearum, trichoderma virens and trichoderma harzianum. The traditional Chinese medicine extract is composed of radix scutellariae, ephedra, tagetes, oleander, ginkgo leaves and sophora flavescens in a mass ratio of 10-16:13-17:7-11:8-12:4-8:3-7. The traditional Chinese medicine extract is obtained by mixing the above-mentioned traditional Chinese medicine materials, adding water and decocting, and then filtering. The compound microbial fertilizer can not only prevent and treat the root rot of angelica, but also significantly improve the yield and quality of angelica.