The invention relates to a strain capable of producing 1,4-beta-D-
xylanase and an application of the strain and can effectively solve the problem about production of 1,4-beta-D-
xylanase to meet demands in industry. The method comprises steps as follows: a culture medium for
enzyme production by
fermentation is inoculated with the strain capable of producing 1,4-beta-D-
xylanase, a
fermentation broth of 1,4-beta-D-xylanase is obtained and centrifuged,
ammonium sulfate is slowly dropwise added to a liquid supernatant and salted out overnight, a
salting-out liquid is centrifuged, precipitates are taken and dissolved with a
buffer solution, packing is performed, a
buffer solution is taken as a balance solution for DAE FF gel
filtration chromatography,
active components are collected, packing is performed again, linear
elution is performed with a balance
buffer solution,
active components are collected, the
mass concentration of
ammonium sulfate in the
active components is adjusted to 40%, packing is performed, an
ammonia sulfate buffer solution is taken as a balance solution for linear
elution, active components are collected, salt is removed through
dialysis, and
dry powder of 1,4-beta-D-xylanase is obtained through freezing and suction
drying. The strain capable of producing 1,4-beta-D-xylanase is a novel strain
Trichoderma citrinoviride HL28-5 and has the
efficacy of producing 1,4-beta-D-xylanase, and a new way of producing 1,4-beta-D-xylanase is exploited.