35 results about "Trichoderma citrinoviride" patented technology

The invention discloses a compound bacterial manure used for prevention and control of cotton verticillium wilt and the preparation method thereof. Materials of the compound bacterial manure consist of Streptomyces aburaviensis, Streptomyces tanashiensis, Brevibacillus agri, Bacillus clausii, Trichoderma citrinoviride, and potassium fulvic acid. Bacteria of the invention can make full use of the interspecific or intraspecific antibiosis, competition, hyperparasite, and bacteriolysis of microbes, and induce cotton to generate the disease resistance ability through secondary metabolite, thereby producing various antibiotic substances such as luteomycin and aburamycin to prevent the pathogenic bacteria of cotton verticillium wilt. The 5 strains used in the invention can be obtained through separation from soil directly; the strains can promote the growth of root, and secrete some secondary metabolites to plant roots while growing on the plant surface, inside the plant or in soil, thereby improving the plant's absorption of nutrients, and promoting the plant growth like fertilizers.

The invention relates to a trichoderma citrinoviride P-T 7987 strain, which is preserved in China General Microbiological Culture Collection Center and is assigned the accession number CGMCC No.10646. The trichoderma citrinoviride P-T 7987 is strong in habitat adaptability, is high in growth speed, is high in sporulation quantity, can be used for inhibiting botrytis cinema, sclerotinia sclerotiorum, pythium aphanidermatum, phytophthora capsici and rhizoctonia solani, can be hyper-parasitized on hypha of the botrytis cinema, the sclerotinia sclerotiorum, the pythium aphanidermatum, the phytophthora capsici and the rhizoctonia solani to rapidly and effectively degrade the vegetable bacteria. The trichoderma citrinoviride P-T 7987 has a potential and developable biological prevention and treatment value.

The invention discloses a preparation method of a microbial inoculum for preventing and curing fungal diseases of plants; the microbial inoculum for preventing and curing fungal diseases of plants isprepared by mixing Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum prepared by the method of the invention is used to carry out control efficiency experiments and theresults show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and the control efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by 11.06% so that the effect for preventing diseases and increasing yield is obvious; thecontrol efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

The invention discloses a banana stem and leaf residue decomposing inoculant for inhibiting fusarium wilt and a preparation method thereof. The banana stem and leaf residue decomposing inoculant is prepared by using trametes versicolor with a cellulose, lignin and hemicellulose decomposing function, trichoderma citrinoviride, aspergillus oryzae, bacillus amyloliquefaciens with a biological control function, and streptomyces pristinaespiralis for compound fermentation. The banana stem and leaf residue decomposing inoculant can effectively accelerate the decaying of banana stem and leaf residues returned to the field, while inhibiting the growth of fusarium wilt of banana and other pathogenic bacteria; the decomposition of the banana stem and leaf residues is accelerated after applying the decomposing inoculant in the field, wherein the weight loss rate of the banana stem and leaf residues is increased remarkably; the tensile force of the banana stem and leaf residues is reduced remarkably; the number of soil pathogens including antagonistic bacillus and actinomycetes is increased remarkably; and the number of pathogenic organisms is reduced remarkably.

The present invention relates to producing micropropagules from Trichoderma citrinoviride EGE-K-130 mould strain isolated from resources of our country and whose strain is molecularly identified, as a bio-control agent by means of an eco nomical production process.

The invention relates to a strain capable of producing 1,4-beta-D-xylanase and an application of the strain and can effectively solve the problem about production of 1,4-beta-D-xylanase to meet demands in industry. The method comprises steps as follows: a culture medium for enzyme production by fermentation is inoculated with the strain capable of producing 1,4-beta-D-xylanase, a fermentation broth of 1,4-beta-D-xylanase is obtained and centrifuged, ammonium sulfate is slowly dropwise added to a liquid supernatant and salted out overnight, a salting-out liquid is centrifuged, precipitates are taken and dissolved with a buffer solution, packing is performed, a buffer solution is taken as a balance solution for DAE FF gel filtration chromatography, active components are collected, packing is performed again, linear elution is performed with a balance buffer solution, active components are collected, the mass concentration of ammonium sulfate in the active components is adjusted to 40%, packing is performed, an ammonia sulfate buffer solution is taken as a balance solution for linear elution, active components are collected, salt is removed through dialysis, and dry powder of 1,4-beta-D-xylanase is obtained through freezing and suction drying. The strain capable of producing 1,4-beta-D-xylanase is a novel strain Trichoderma citrinoviride HL28-5 and has the efficacy of producing 1,4-beta-D-xylanase, and a new way of producing 1,4-beta-D-xylanase is exploited.

The present invention relates to the field of microorganisms and water treatments, and discloses Trichoderma citrinoviride M-13 with the preservation number of CGMCC No.8332, wherein the microorganism can be used for reducing or absorbing Cr<6+> and treating the water body containing Cr<6+>, provides a restoration effect for the contaminated water body, and can be used for sewage treatments. According to the invention, the microorganism is used for sewage treatments, and has characteristics of simple process, high treatment efficiency, low cost and good application prospects.

The invention relates to an application of penicilliumenol E1 from trichoderma citrinoviride in preparation of anti-breast cancer drugs. The compound has the structural characteristics as follows: the compound comprises a molecular skeleton of pentacyclic amid adn a carbonyl group connected with a saturated fat long chain with ten carbon molecules, N is connected with one methyl, and one molecule contains two hydroxyl groups. Experiments prove that alkaloid compounds have better inhibition activity for breast cancer cells and can be used for anti-tumor research as a substance for preparation of drugs for inhibiting proliferation of breast cancer cells or anti-breast cancer drugs.

The invention discloses application of trichoderma citrinoviride-derived penicillium enol E1 to a liver cancer aspect. The compound has the following structure characteristics: a molecular skeleton contains one pentacyclic amide, one carbonyl is connected with one 10-carbon saturated fat long chain, N is connected with one methyl and two hydroxyl groups exist in the molecule. Experiments prove that the alkaloid compound has high inhibition activity on liver cancer cells, can be used for preparing medicines for inhibiting proliferation of the liver cancer cells or medicines for resisting liver cancer, and is applied to anti-tumor research.

The invention relates to an application of penemenol E1 derived from trichoderma citrinoviride in an aspect of resisting nasopharynx cancer. The compound has the following structural characteristics: the compound contains the molecular skeleton of a pentacyclic amide, the compound contains a carbonyl group which is linked to a decane saturated fat long chain, a methyl group is linked to N, and two hydroxyl groups exist in molecules. Experiments prove that the alkaloid compound has a relatively good inhibitory activity on nasopharynx cancer; and the alkaloid compound can be used for preparing medicines for inhibiting proliferation of nasopharynx cancer cells or medicines for resisting the nasopharynx cancer, and the alkaloid compound is applicable to anti-tumor researches.

The invention relates to penem enol E1 derived from trichoderma citrinoviride and application in preparation of an anti-oral epidermoid carcinoma drug. The compound is structurally characterized by containing a molecular skeleton of pentacyclic amide, a carbonyl connected with a long n-decyl saturated aliphatic chain, a methyl connected to N and molecules existing in two hydroxyl groups. An experiment proves that the alkaloid compound has good oral epidermoid carcinoma inhibiting activity. The penem enol E1 can serve as a drug for inhibiting oral epidermoid carcinoma cell proliferation or the anti-oral epidermoid carcinoma drug for antitumor research.