Trichoderma citrinoviride and application thereof

A technology of Trichoderma citrifolia and fungal cell wall, applied in the field of microorganisms, can solve the problems of environmental impact of pesticides, large amount of chemical pesticides, etc., and achieve the effect of protecting the environment and reducing damping-off of peppers

Active Publication Date: 2016-01-06
SHANGHAI WANLIHUA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of Trichoderma strain with high spore production and efficient prevention and treatment of capsicum damping-off, so

Method used

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  • Trichoderma citrinoviride and application thereof
  • Trichoderma citrinoviride and application thereof
  • Trichoderma citrinoviride and application thereof

Examples

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Embodiment Construction

[0042] The invention will be explained in more detail by means of the following examples. The following examples are illustrative only, and it should be understood that the present invention is not limited by these examples.

[0043] strain screening

[0044] Weigh 1g of soil sample into 10ml of water, mix it on a vortex instrument, and then carry out gradient dilution to 10 -5 , take 1ml and add it to the surface of the Trichoderma selective medium TSM medium, spread evenly, and culture the medium statically at 25° C. for 5-7 days. According to the shape, color and size of the fungal colonies screened on the plate, Trichoderma colonies were selected and transferred to PDA medium for purification, cultivation and further identification.

[0045] TSM medium formula (unit is g / l): MgSO 4 ·7H 2 O0.2g, KH 2 PO 4 0.9g, KCl0.15g, NH 4 NO 3 1.0g, glucose 3g, chloramphenicol 0.25g, methylsulfonate 0.3g, pentachloronitrobenzene 0.2g, rose bengal 0.15g, agar 20g.

[0046] Strai...

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Abstract

The invention discloses a trichoderma citrinoviride strain. The trichoderma citrinoviride strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the trichoderma citrinoviride strain is CGMCC No. 8722. The trichoderma citrinoviride strain has the advantages that the trichoderma citrinoviride strain dissolves pathogenic fungus cell walls through the self-produced fungal cell wall degrading enzyme, the trichoderma citrinoviride strain seeps into the cells of pathogenic fungi to form a large amount of branches and sexual structures, the growth of the pathogenic fungi is inhibited, and the pathogenic fungi die; the pathogenic fungi of pepper damping-off can be efficiently inhibited by the trichoderma citrinoviride strain, pepper damping-off is reduced, and the trichoderma citrinoviride strain can replace chemical pesticides to inhibit pepper damping-off while environmental protection is achieved.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a trichoderma fungus with high antagonistic effect on pepper seedling damping-off. Background technique [0002] Capsicum damping-off is a disease that mainly harms seedlings. Its symptoms are: the damage before emergence can lead to rotten seeds and dead seedlings; Thin thread. In the later stage, the seedlings are lodging, and when the humidity is high, white cotton flocs are densely grown on the diseased part. In addition to the damage to capsicum and solanaceous vegetables, the disease can also affect vegetable seedlings such as melons, lettuce, celery, cabbage, cabbage, radish, and onion. The pathogen of the disease is Pythiumaphanidermatum (Eds.) Fitzp., which belongs to the flagellate subphylum fungus, and is the pathogen of damping-off disease in vegetable seedling stage. Seedlings cause great harm. [0003] In order to prevent this fungal disease, chemical pesticide...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 干华磊吴紫燕黄杰锋陈娟王承芳谢俊旷文丰毛伟力
Owner SHANGHAI WANLIHUA BIOTECH
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