A kind of trichoderma longibranchus and application thereof

A technology of Trichoderma longum and fungal cell wall, applied in the field of microorganisms, can solve problems such as multi-pesticide environment, adverse effects, etc., and achieve the effects of reducing harm, improving quality, and reducing the occurrence of strawberry anthracnose

Active Publication Date: 2018-07-10
SHANGHAI WANLIHUA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a Trichoderma strain with high spore production and high efficiency for preventing and treating strawberry anthracnose, so as to solve the problem that the current control of strawberry anthracnose requires a large amount of chemical pesticides and the pesticides have adverse effects on the environment

Method used

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  • A kind of trichoderma longibranchus and application thereof
  • A kind of trichoderma longibranchus and application thereof
  • A kind of trichoderma longibranchus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, the screening and cultivation of Trichoderma longibrachiata

[0050] In the present embodiment, relevant Trichoderma longibrachiae screening and culture method thereof are as follows:

[0051] strain screening

[0052] Weigh 1g of soil sample into 10ml of water, mix it on a vortex instrument, and then carry out gradient dilution to 10 -5 , take 1ml and add it to the surface of the Trichoderma selective medium TSM medium, spread evenly, and culture the medium statically at 25° C. for 5-7 days. According to the shape, color and size of the fungal colonies screened on the plate, Trichoderma colonies were selected and transferred to PDA medium for purification, cultivation and further identification;

[0053] Wherein, the formula (unit is g / l) of described TSM medium: MgSO 4 ·7H 2 O 0.2g, KH 2 PO 4 0.9g, KCl0.15g, NH 4 NO 3 1.0g, glucose 3g, chloramphenicol 0.25g, methylsulfonate 0.3g, pentachloronitrobenzene 0.2g, rose bengal 0.15g, agar 20g.

[0...

Embodiment 2

[0057] Embodiment 2, the cultivation of Trichoderma longibrachiata

[0058] In this embodiment, 100 mL of PDB culture solution was added to each 250 mL Erlenmeyer flask, and 1 mL of spore suspension was inoculated in each bottle. Cultivate the bacterial strain at a temperature of 25° C. and shake at 150 r / min, and a large amount of spores of Trichoderma longibranchus strain can be harvested on the 7th day. Add an equal volume of diatomaceous earth to the spores, filter, pulverize and dry for later use.

[0059] Among them, such as Figure 1-Figure 3 Colony morphology and conidiophores, phialides straight, and ampoule-shaped diagrams of Trichoderma longibrachiata strains shown in .

[0060] which from Figure 1-Figure 3 It can be seen that the conidia on the PDA medium are green, oval in shape, (2.5-)3.5-4.7(-7.8)×(2.0-)2.3-3.0(-4.0)μm in size, and the length is the same as The width ratio is (0.9-)1.5-2.0(-2.7), smooth;

[0061] Chlamydospores are abundant, terminal chl...

Embodiment 3

[0064] Embodiment 3, described Trichoderma longibrachiae is used for preventing and treating strawberry anthracnose

[0065] In this example, the strain preparation of Trichoderma longibrachiae described in the present invention, the existing pyraclostrobin, azoxystrobin and difenoconazole were divided into four experimental groups to prevent and control strawberry anthracnose.

[0066] Specific experimental methods include:

[0067] Mix the soil 7-10 days before planting, and after transplanting and surviving, irrigate the roots three times with an interval of 10-15 days;

[0068] After pulling out a large number of stolons, spray the treatment once;

[0069] Each treatment plot was randomly sampled at 5 points, and the blades, petioles and stolons of the established strawberry seedlings were examined 7 days after the last spraying, and the disease progression was recorded, and then the disease index was calculated, and the relative control effect was calculated according ...

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Abstract

The invention discloses a Trichoderma longibranchiatum. The Trichoderma longibranchiatum has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 14, 2014, and the preservation number is CGMCC No.8723. The fungal cell wall degrading enzymes produced by Trichoderma longibrachiae of the present invention such as chitinase, dextranase, etc., these enzymes can dissolve the cell wall of pathogenic bacteria and penetrate into its cells to form a large amount of branches and sexual structures, so that The growth of pathogenic fungi is inhibited and dies, thereby realizing the effect of preventing and treating strawberry anthracnose. The trichoderma longibrachiae of the invention can efficiently inhibit the pathogenic fungus of strawberry anthracnose, reduce the occurrence of strawberry anthracnose, and replace chemical pesticides to inhibit strawberry anthracnose.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Trichoderma neophytum strain with high antagonistic organisms to strawberry anthracnose. Background technique [0002] Strawberry anthracnose is one of the main diseases of strawberry seedlings, and it is a universal fungal disease. Strawberry anthracnose mainly occurs at the seedling stage and initial stage of planting, and mainly harms stolons, petioles, leaves, stipules, petals, calyxes and fruits. Under the climate conditions of high temperature and rainy summer, anthracnose occurs seriously, causing a large number of dead seedlings and a low seedling rate, which is a worldwide problem in strawberry seedling cultivation and seriously affects the economic benefits of strawberry planting. [0003] The disease is caused by the infection of the fungus Deuteromycetes Colletotrichum fragariae Brooks, whose sexual stage is Glomerella fragariae of the Ascomycota subphylum. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 郭玉婷吴紫燕陈娟黄杰锋陈晨王承芳王军旷文丰毛伟力
Owner SHANGHAI WANLIHUA BIOTECH
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