A kind of trichoderma longibranchus and application thereof
A technology of Trichoderma longum and fungal cell wall, applied in the field of microorganisms, can solve problems such as multi-pesticide environment, adverse effects, etc., and achieve the effects of reducing harm, improving quality, and reducing the occurrence of strawberry anthracnose
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Embodiment 1
[0049] Embodiment 1, the screening and cultivation of Trichoderma longibrachiata
[0050] In the present embodiment, relevant Trichoderma longibrachiae screening and culture method thereof are as follows:
[0051] strain screening
[0052] Weigh 1g of soil sample into 10ml of water, mix it on a vortex instrument, and then carry out gradient dilution to 10 -5 , take 1ml and add it to the surface of the Trichoderma selective medium TSM medium, spread evenly, and culture the medium statically at 25° C. for 5-7 days. According to the shape, color and size of the fungal colonies screened on the plate, Trichoderma colonies were selected and transferred to PDA medium for purification, cultivation and further identification;
[0053] Wherein, the formula (unit is g / l) of described TSM medium: MgSO 4 ·7H 2 O 0.2g, KH 2 PO 4 0.9g, KCl0.15g, NH 4 NO 3 1.0g, glucose 3g, chloramphenicol 0.25g, methylsulfonate 0.3g, pentachloronitrobenzene 0.2g, rose bengal 0.15g, agar 20g.
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Embodiment 2
[0057] Embodiment 2, the cultivation of Trichoderma longibrachiata
[0058] In this embodiment, 100 mL of PDB culture solution was added to each 250 mL Erlenmeyer flask, and 1 mL of spore suspension was inoculated in each bottle. Cultivate the bacterial strain at a temperature of 25° C. and shake at 150 r / min, and a large amount of spores of Trichoderma longibranchus strain can be harvested on the 7th day. Add an equal volume of diatomaceous earth to the spores, filter, pulverize and dry for later use.
[0059] Among them, such as Figure 1-Figure 3 Colony morphology and conidiophores, phialides straight, and ampoule-shaped diagrams of Trichoderma longibrachiata strains shown in .
[0060] which from Figure 1-Figure 3 It can be seen that the conidia on the PDA medium are green, oval in shape, (2.5-)3.5-4.7(-7.8)×(2.0-)2.3-3.0(-4.0)μm in size, and the length is the same as The width ratio is (0.9-)1.5-2.0(-2.7), smooth;
[0061] Chlamydospores are abundant, terminal chl...
Embodiment 3
[0064] Embodiment 3, described Trichoderma longibrachiae is used for preventing and treating strawberry anthracnose
[0065] In this example, the strain preparation of Trichoderma longibrachiae described in the present invention, the existing pyraclostrobin, azoxystrobin and difenoconazole were divided into four experimental groups to prevent and control strawberry anthracnose.
[0066] Specific experimental methods include:
[0067] Mix the soil 7-10 days before planting, and after transplanting and surviving, irrigate the roots three times with an interval of 10-15 days;
[0068] After pulling out a large number of stolons, spray the treatment once;
[0069] Each treatment plot was randomly sampled at 5 points, and the blades, petioles and stolons of the established strawberry seedlings were examined 7 days after the last spraying, and the disease progression was recorded, and then the disease index was calculated, and the relative control effect was calculated according ...
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