The present invention provides a method for manufacturing fungal pharmaceutical composition, used for extracting a glycosaminoglycan fiber from a fungal cell wall. Differing from the glycosaminoglycan fiber produced by using a fabrication method proposed by Taiwan patent No. 442496 showing many drawbacks including low extraction percentage, coarse fiber, and having light-yellow color, the glycosaminoglycan fiber manufactured by using this novel method reveals the advantages of high extraction percentage, fine fibers, and showing white color. So that, the novel glycosaminoglycan fiber produced by using the present invention's method is suitable for being processed to an excipient. Moreover, because a variety of experimental results have proved that the glycosaminoglycan fiber produced by using the present invention's method possesses good adsorption ability of tissue fluid and moisture retention ability, this novel glycosaminoglycan fiber is also suitable for being processed to a skin dressing, an artificial skin, or a hydrate mask.