Trichoderma harzianum strain

A technology of strains and fungi, applied in the directions of fungicides, fungi, biocides, etc., can solve the problems of difficult industrialized mass production, poor adaptability to external environment, small spore production of Trichoderma, and achieve efficient control and wide application range. , easy to cultivate effect

Inactive Publication Date: 2013-04-17
SHANGHAI WANLIHUA BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As also found in the research, Trichoderma also has the effect of antagonizing microbial pathogens, and it has a strong inhibitory effect on soil-borne plant pathogenic fungi, but in time cu

Method used

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  • Trichoderma harzianum strain
  • Trichoderma harzianum strain
  • Trichoderma harzianum strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: to the field control of capsicum wilt:

[0042] Control of Capsicum Fusarium Wilt with Trichoderma harzianum CGMCC No.5202

[0043] experiment method

[0044] A total of 3 times were applied during the experiment, the first time: planting (April 25, 2010); the second time: flowering period (May 11, 2010); the third time: initial fruiting period (May 27, 2010 day). On April 25, 2010, the plants were planted, and the roots were irrigated on the same day, and each root was irrigated with 500mL of Trichoderma harzianum preparation.

[0045] Description of experimental conditions:

[0046] The field specifications, varieties and fertilizer and water management conditions are the same. And there was no rainfall on the day after each spraying, and no fertilization, irrigation and other pest control were carried out during the test period.

[0047] Data test method:

[0048] Disease severity grading survey. Random sampling was adopted in each plot, ...

Embodiment 2

[0054] Embodiment 2: Petri dish confrontation test:

[0055] Carry out the plate confrontation test between Trichoderma harzianum strain CGMCC No.5202 and cucumber wilt and corn stalk rot: use a puncher to punch the Trichoderma harzianum strain and the pathogen strain into 5mm 2 Row's mycelium cake, respectively inserts the two ends of the PDA culture medium of 9cm, only inoculates pathogenic bacteria, does not inoculate Trichoderma as control. Measure the colony diameter to calculate the antibacterial rate. The antibacterial rates were 78.44% and 77.37%, respectively.

[0056] From the above experimental results, we found that the Trichoderma harzianum strain of the present invention has an efficient control effect on plant diseases caused by fungi.

[0057] The Trichoderma harzianum strain (Trichoderma harizianum) described in the present invention was preserved on August 31, 2011 in the General Microorganism Center of the China Microbial Culture Collection Management Co...

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Abstract

The invention provides a Trichoderma harzianum strain which is preserved in the general microbiology center of management committee of microorganisms deposit, with an accession number being CGMCC No. 5202. The Trichoderma harzianum strain can dissolve cell walls of pathogen by using self-producing fungal cell wall degrading enzymes and penetrate into the pathogen cells, so that the growth of the pathogenic fungi is inhibited and then dead, thereby realizing prevention and treatment effects of diseases, and highly-efficiently preventing and treating plant diseases caused by fungi. The Trichoderma harzianum strain provided by the invention is large in sporulation amount, easy to cultivate, suitable for industrial mass production, wide in using range and strong in practicality.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a trichoderma harzianum strain with high resistance to various soil-borne diseases. Background technique [0002] Under the large-scale production mode of modern melons, fruits and vegetables, diseases caused by fungi have become the main problems affecting plant quality, yield and preservation. These pathogenic bacteria are facultative parasitic fungi with a wide range of hosts. They infect a wide range of pathogens. They can parasitize a variety of fruits, vegetables and flowers. Not only can they infect fruit stems, new shoots and young leaves during plant growth, Harm inflorescences, young fruits, and fruits that are about to mature, and often become the main disease that causes fruit rot during storage, transportation, and sales due to the latent existence of the pathogen. Such as Botrytis cinerea, which is caused by the infection of Deuteromycotina, Hyphospora, Hyphospora, Botrytis ci...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 旷文丰王承芳张钰王军胡曲瑞毛伟力
Owner SHANGHAI WANLIHUA BIOTECH
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