Technology for extracting glutathione peroxidase with biological method

A glutathione peroxidation and bio-extraction technology, applied in biochemical equipment and methods, enzymes, enzymes, etc., can solve problems such as poor, difficult extraction methods of glutathione peroxidase, cumbersome effects, etc. , to achieve the effect of rich selenium content

Inactive Publication Date: 2010-03-17
崔所亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: the present invention provides a biological process for extracting glutathione peroxidase, the purpose of which is to solve the problems of difficulty, tediousness and poor effect of the previous methods for extracting glutathione peroxidase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a. Select an environment with a temperature of 8°C, a sunlight intensity of 3500LUX, and an environment with a soil moisture content of 50%, and select a selenium-rich moss with a thickness of 8 cm as the culture substrate; this environment can be simulated in a greenhouse or directly. Select in the valley of Baxian County, Qinling Town, Qinling Mountains at an altitude of 2,700 meters, and the sowing time is selected in the first ten days of the month in April of the lunar calendar, just before and after Qingming Festival, because in this period, the environment in this area is closer to the environment required by the present invention, Moreover, the average annual rainfall in this area is between 600 and 800 mm, so the humidity can meet the requirements, and it is relatively close to Ziyang, Shaanxi, the selenium capital of our country, so the selenium content in this area is also quite rich, so the moss in this area also contains There is a lot of selenium, so it is ...

Embodiment 2

[0030] a. Select an environment with a temperature of 9°C, a sunlight intensity of 3500LUX, and an environment with a soil moisture content of 55%, and select a selenium-rich moss with a thickness of 10 cm as the culture substrate; this environment can be simulated in a greenhouse or directly. Select in the valley of Baxian County, Qinling Town, Qinling Mountains at an altitude of 2,700 meters, and the sowing time is selected in the first ten days of the month in April of the lunar calendar, just before and after Qingming Festival, because in this period, the environment in this area is closer to the environment required by the present invention, Moreover, the average annual rainfall in this area is between 600 and 800 mm, so the humidity can meet the requirements, and it is relatively close to Ziyang, Shaanxi, the selenium capital of our country, so the selenium content in this area is also quite rich, so the moss in this area also contains There is a large amount of selenium,...

Embodiment 3

[0038]a. Select an environment with a temperature of 10°C, a sunlight intensity of 4000LUX, and an environment with a soil moisture content of 60%, and select a selenium-rich moss with a thickness of 15 cm as the culture substrate; this environment can be simulated in a greenhouse or directly. Select in the valley of Baxian County, Qinling Town, Qinling Mountains at an altitude of 2,700 meters, and the sowing time is selected in the first ten days of the month in April of the lunar calendar, just before and after Qingming Festival, because in this period, the environment in this area is closer to the environment required by the present invention, Moreover, the average annual rainfall in this area is between 600 and 800 mm, so the humidity can meet the requirements, and it is relatively close to Ziyang, Shaanxi, the selenium capital of our country, so the selenium content in this area is also quite rich, so the moss in this area also contains There is a lot of selenium, so it is...

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Abstract

The invention provides a technology for extracting glutathione peroxidase with a biological method, specially comprising the steps of: a. in the environment that the temperature is 8-10DEG C and sun illuminance is 3500-4000 LUX, selecting selenium rich moss with the depth of 8-15cm as culture base material, and guaranteeing the water content of the soil for growing the selenium rich moss to be 50%-65%; b. burying seeds of rubus crataegifolius bge into the selenium rich moss of the step a according to plant distance of 10-15cm and row distance is 10-15cm; c. after three moths to three and a half moths, picking leaves of the rubus crataegifolius bge to be standby; d. preparing solution with water, salt and pure selenium according to the mass ratio of 100:2:1; and e. dipping the leaves in thestep c with the solution in the step d at normal temperature for 0-20 minutes, washing out impurity on the leaves, drying and grinding. The method is a pure biological extraction method; and comparedwith the chemical extraction method, the method is easily operated, saves labor power and physical resources, and has a good effect.

Description

Technical field: [0001] The invention belongs to the field of biological extraction, in particular to a process for extracting glutathione peroxidase by a biological method. Background technique: [0002] Glutathione peroxidase (GSH-Px) is an important peroxide-decomposing enzyme widely present in the body. Selenium is a component of the glutathione peroxidase system, which can catalyze glutathione (GSH) into oxidized glutathione (GSSG), reducing toxic peroxides to non-toxic hydroxyls Compounds, while promoting the decomposition of hydrogen peroxide (H2O2), thereby protecting the structure and function of the cell membrane from peroxide interference and damage. The active center of glutathione peroxidase (GSH-Px) is selenocysteine, and its activity can reflect the selenium level in the body. [0003] The GSH-Px enzyme system mainly includes 4 different GSH-Px, which are cytoplasmic GSH-Px, plasma GSH-Px, phospholipid hydroperoxide GSH-Px and gastrointestinal specific GSH-P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08
Inventor 崔所亮王翠霞
Owner 崔所亮
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