88 results about "Oxidized Glutathione" patented technology

The invention relates to the field of pharmaceutical synthesis and discloses a linaclotide synthesis method. The linaclotide synthesis method includes: performing solid-phase synthesis to obtain linaclotide resin with an N terminal, a Thr side chain, a Cys side chain, an Asn side chain, a Tyr side chain and a Glu side chain of an amino acid sequence shown in SEQ ID NO:1 coupled with protecting groups and with a C terminal coupled with a resin solid-phase carrier, cracking to remove the protecting groups and the resin solid-phase carrier prior to carrying out oxidizing reaction by the aid of a GSH (glutathione)/GSSH (oxidized glutathione) oxidization system to obtain a crude linaclotide product, and purifying the crude linaclotide product so that linaclotide is obtained. By the method, an Mmt protecting group is used for protecting a cysteine side chain, crude linear linaclotide peptide is synthesized by a one-by-one coupling mode, and the linaclotide is obtained by oxidization by the aid of the GSH /GSSH oxidization system. Compared with existing methods, the linaclotide synthesis method has the advantages that purity of the crude linear peptide is improved, the oxidization step can be performed without purification, and purity and yield of the crude linaclotide product are remarkably improved.

Methods of modulating, adjusting, and maintenance of the glutathione redox status of an individual, or cell, tissues, bodily fluids, or body compartments of the individual, are disclosed, as are compositions suitable for such modulating, adjusting, and maintenance. The modulation or adjustment is achieved by altering the amounts of reduced glutathione versus oxidized glutathione in the individual, or cells, tissues, bodily fluids, or body compartments of the individual. Modulation, adjustment, and maintenance of the redox status of an individual enables treatment, prevention or suppression of diseases or symptoms associated with diseases. These methods are achieved by using quinone derivatives.

The present invention relates to a kit for the treatment of cancer comprising (a) a container for containing a first compound (i) or a precursor thereof, said first compound or precursor being a compound that oxidizes glutathione (GSH); (b) a container for containing a second compound (ii) or a precursor thereof, said second compound or precursor being a compound that forms an adduct or conjugate with GSH; (c) a container for containing a third compound (iii) or a precursor thereof, said third compound or precursor being a compound that inhibits the rate-limiting enzyme of GSH biosynthesis, gamma-glutamylcysteine synthetase (GCS); and (d) a container for containing a fourth compound (iv) or a precursor thereof, said fourth compound or precursor being a compound that inhibits the enzyme responsible for the conversion of GSSG to GSH, glutathione reductase (GR).

The invention provides methods, compositions and kits relating to hyaluronan based matrices using oxidized glutathione as a crosslinking agent.

The present invention provides crystals of glutathione oxidized n hydrate (wherein n is an integer or a fraction of not less than 0 and less than 8) useful, for example, as final products of, as raw materials for, or as intermediates of health foods, pharmaceuticals, cosmetics or the like, and a process for producing crystals of glutathione oxidized, which comprises a step of crystallizing glutathione oxidized from a mixture of an aqueous solution containing glutathione oxidized and a water-miscible organic solvent and which is suitable for large-scale synthesis or industrialization.

The present invention is directed to a method for decreasing inflammation and oxidative stress in a mammal comprising; administration to a mammal a composition comprising a glucose anti-metabolite; and wherein said composition comprises amounts of the glucose anti-metabolite sufficient to decrease a level of an oxidized glutathione and/or increase the ration of reduced glutathione to oxidized glutathione in the blood of the mammal subsequent to administration of the glucose anti-metabolite.

The present invention relates to a composite for the treatment of a variety of medical conditions, the composite comprising an oxidized glutathione-based compound, which has a disulfide bond, and a metal material, in particular where the metal is either platinum or palladium. The oxidized glutathione-based compound and metal material can be present in a ratio of 3000 to 1 and preferably 1000 to 1. The oxidized glutathione-based compound can be oxidized glutathione itself or salts or derivatives. A feature of the invention is that the composite has a more stabilized disulfide bond than the oxidized glutathione-based compound itself. Methods for preparing the composite are provided, such methods being beneficial in that the composite is provided in high yields and at high purity. Methods for treating various medical conditions with the composites of the present invention are also disclosed.

The present invention relates to a composite for the treatment of a variety of medical conditions, the composite comprising an oxidized glutathione-based compound, which has a disulfide bond, and a metal material, in particular where the metal is either platinum or palladium. The oxidized glutathione-based compound and metal material can be present in a ratio of 3000 to 1 and preferably 1000 to 1. The oxidized glutathione-based compound can be oxidized glutathione itself or salts or derivatives. A feature of the invention is that the composite has a more stabilized disulfide bond than the oxidized glutathione-based compound itself. Methods for preparing the composite are provided, such methods being beneficial in that the composite is provided in high yields and at high purity. Methods for treating various medical conditions with the composites of the present invention are also disclosed.

The invention relates to a method for producing an intelligent degradable edible film by using pea starch. The method comprises the following steps: S1, component blending, namely adding 100-120kg ofstarch into 1000L of purified water under stirring, carrying out dispersion and uniform mixing, then, carrying out further stirring for 10-15min, then, adding 40-60L of plasticizer, carrying out stirring for 10-15min, then, adding 12-18kg of fortifier, 1-1.5kg of anhydrous calcium chloride, 500-800g of plant extracts, 500-800g of natural colorant and 3-5kg of oxidized glutathione, and carrying outstirring for 30-40min; S2, homogeneous degasification; S3, tape casting; S4, drying; and S5, film uncovering, mechanical film winding, packaging and storage. The film material disclosed by the invention is capable of realizing identification of quality change of a food through color development and has good antibacterial properties, mechanical properties, oil resistance, humidity resistance and oxygen resistance. The method can be applied to packaging of various materials such as foods, health products, medicines and feeds.

The invention provides a renaturation liquid for recombining a chicken alpha interferon inclusion body as well as a preparation method and an application thereof. The renaturation liquid mainly comprises arginine, EDTA (Ethylene Diamine Tetraacetic Acid), oxidized glutathione, Tris and glycerol, wherein the concentration of each component is as follows: 0.2-0.8 M of arginine, 0.5-2 mM of EDTA, 0.3-0.9 mM of toxidized glutathione, 60-100 mM of Tris and 15-30% (v/v) of glycerol; and the pH (Potential of Hydrogen) of the renaturation liquid is 8.0-8.5. The renaturation liquid is prepared by dissolving in distilled water and utilizing HCl to adjust the pH value; the renaturation liquid has an application prospect on the aspect of recombining the chicken alpha interferon inclusion body; and the renaturation liquid has a reasonable proportion for components to carry out a three-step washing and purifying and drip sample injection primary renaturation process method on the inclusion body to better separate a bacterial protein with the inclusion body, so that the renaturation rate of the protein is greatly improved.

The invention discloses a glycine-containing feed additive and applications thereof. The invention aims to protect a feed additive taking glycine as an effective component and following applications 1)-5) of glycine or the feed additive: 1) an application of improving the growth performance of weaned piglets; 2) an application of increasing the content of free glycine in the plasma of the weaned piglets; 3) an application of increasing the content of reduced glutathione in the plasma of the weaned piglets; 4) an application of reducing the contents of glutamine, ammonia, urea and oxidized glutathione in the plasma of the weaned piglets; and 5) an application of reducing the ratio of oxidized glutathione to reduced glutathione in the plasma of the weaned piglets. By adding glycine in the daily ration, the average daily feed intake, average daily gain and feed conversion efficiency of weaned piglets of 8-15kg can be obviously increased. Tests show that by adding 0.75% or 1.5% of glycine into the corn-bean meal daily ration for the weaned piglets of 8-15kg, the best growth performance of the weaned piglets can be favorably exerted.

The composition, in accordance with the present invention, for producing a plant body having an improved sugar content includes glutathione, a polynucleotide encoding γ-glutamylcysteine synthetase, or a polynucleotide encoding glutathione-binding plastid type fructose-1,6-bisphosphate aldolase. The composition preferably includes oxidized glutathione. This allows provision of a composition for easily producing a plant body having an improved sugar content.

The invention provides a glutathione reductase determination kit, and a preparation method and applications thereof, and relates to the technical field of biochemical reagent determination. The kit includes a reagent R1 and a reagent R2; the reagent R1 includes a phosphate buffer solution, ascorbic acid oxidase, pyruvate oxidase, potassium ferrocyanide, oxidized glutathione, MgCl2, FAD, a preservative and a surfactant; and the reagent R2 includes a glycine buffer solution, NADPH, the preservative and a protective agent. Through the adding of EGTA and dithiothreitol in the reagents, the linearrange of the kit can be enlarged, and the stability of the kit can be enhanced. The preparation method and applications of the kit are also provided; and the kit can accurately determine glutathione reductase.

The invention discloses a cleaning liquid after chemical mechanical polishing and a preparation method thereof. The cleaning liquid comprises the following raw material components in mass fraction: 0.01%-25% of alkali, 0.01%-30% of alcohol amine, 0.001%-1% of an antioxidant, 0.01%-0.1% of polypeptide, 0.01%-0.1% of amino acid, 0.01%-10% of a corrosion inhibitor, 0.01%-10% of a chelating agent, 0.01%-5% of a surfactant, 28.9-89.9% of water, based on 100% in total, wherein the amino acid is a combination of histidine and cysteine, and the polypeptide is reduced glutathione (peptide A) and oxidized glutathione (peptide B). The cleaning liquid is stronger in cleaning ability, is lower in corrosion rate, is stronger in BTA removal ability, is better in stability, and can realize the effects of cleaning, corrosion inhibition and BTA removal at the same time.

The invention discloses a compounding method for glutathione and belongs to the technical field of cosmetics and medicines. The method comprises the following steps: protecting L-cystine with phthalicanhydride (called as phthalic anhydride for short); preparing acyl chloride and condensing acyl chloride with glycine protected with hexamethyldisilazane (called as HMDS for short), thereby acquiringcysteine glycine dipeptide protected with phthalic anhydride; removing the phthalic anhydride protecting group, thereby acquiring the cysteine glycine dipeptide; protecting glutamic acid with phthalic anhydride and then reacting with acetic anhydride, thereby acquiring anhydride anhydride; reacting with cysteine glycine dipeptide, thereby acquiring tripeptide; and removing phthalic anhydride protection, thereby acquiring oxidized glutathione, and then reducing S-S bond, thereby acquiring the reduced glutathione GSH. According to the method, a chemical synthesis method is adopted for synthesizing the oxidized and reduced glutathione, the raw materials are low in cost, the yield is high, the high-purity products can be acquired and the method can meet the requirement for industrial production.

A shoot of a plant is cultivated under the presence of glutathione, so that the shoot is rooted. It is possible to cultivate the shoot under the presence of glutathione by use of a rooting medium including glutathione or by contacting a solution including glutathione with the shoot. Oxidized glutathione is preferably used as glutathione. By promoting rooting of the shoot of the plant, a rooting rate of the shoot of the plant is improved. This improves productivity of a clone seedling.

The invention discloses a method for screening glutathione reductase inhibitors based on fluorescence recovery of carbon dots. Yellow fluorescence carbon dots for Ag<+> ion specific quenching serve asa fluorescence probe; glutathione reductase can perform catalytic reduction on oxidized glutathione to produce reduced glutathione in the presence of a coenzyme NADPH (Nicotinamide Adenine Dinucleotide Phosphate), and the reduced glutathione can perform competitive binding with the Ag<+> ion so as to further recover fluorescence of the carbon dots; and the glutathione reductase treated and inactivated by the glutathione reductase inhibitor cannot perform catalytic reduction on the oxidized glutathione, therefore, the fluorescence of the carbon dots cannot be recovered. On this basis, screening of the glutathione reductase inhibitors can be realized by virtue of the fluorescence recovery situation. The method for screening glutathione reductase inhibitors disclosed by the invention has thecharacteristics of being high in sensitivity, excellent in selectivity, low in cost, low in toxicity, environment-friendly, high in stability and the like, and has significances for screening relateddrugs based on the glutathione reductase inhibitor.

The invention relates to a method for detecting reduced glutathione (GSH) and/or oxidized glutathione (GSSG) based on a surface enhanced Raman spectroscopy, and also relates to a kit for detecting GSHand/or GSSG. The detection method and kit are simple in operation, high in detection speed and high in sensitivity, and can be well used for determining the content of GSH and GSSG in an alkylating agent infected cell sample, thereby rapidly screening and detecting an alkylating agent.

The invention provides a method for separating and purifying reduced glutathione from a solution containing glutathione. The method includes the steps of firstly, adding an oxidizing agent to the glutathione solution so that glutathione can be oxidized into oxidized glutathione; secondly, adding acid to the solution, regulating pH to 1.0-4.0, and conducting centrifugation or filtration; thirdly, processing the centrifuged or filtered solution obtained in the second step through ion exchange resin and adsorption resin, and obtaining oxidized glutathione eluant; fourthly, reducing the eluant obtained in the third step through a reducing agent to obtain a glutathione solution, desalting the reduced glutathione solution through adsorption resin, and conducting concentration, crystallization and drying to obtain glutathione. The method is easy and convenient to operate, low in pollution, free of low temperature and nitrogen protection, low in energy consumption, mild in reaction condition and suitable for industrialized production, and the purity of the obtained solid reduced glutathione is larger than 99%.