Method for producing clone seedlings

Abstract

A shoot of a plant is cultivated under the presence of glutathione, so that the shoot is rooted. It is possible to cultivate the shoot under the presence of glutathione by use of a rooting medium including glutathione or by contacting a solution including glutathione with the shoot. Oxidized glutathione is preferably used as glutathione. By promoting rooting of the shoot of the plant, a rooting rate of the shoot of the plant is improved. This improves productivity of a clone seedling.

Description

TECHNICAL FIELD[0001]The present invention relates to a method for producing a clone seedling, and specifically relates to (i) a method for producing a clone seedling by rooting a shoot of a plant and (ii) a rooting medium which can be suitably used for the method.BACKGROUND ART[0002]A step of mass-producing plant bodies (seedlings) having a trait appropriate for a purpose and being homogeneous in quality is unavoidably required in industrially using the plant bodies for the purpose of agricultural production, afforestation, breeding, etc. A conventional cutting propagation and a tissue culture method created by recent development in biotechnology are useful as means for mass-producing plant bodies. Employing those methods makes it possible not only to merely mass-produce seedlings, but also to rapidly produce a large amount of plant bodies having the same trait, i.e., clone seedlings. In particular, in order to mass-produce an excellent line of a genetically modified tree, it is ne...

AI Technical Summary

Benefits of technology

[0037]According to the present invention, a shoot of a plant is cultivated under the presence of glutathione, so that rooting of the shoot is promoted and therefore a rooting rate is improved. This improves productivity of clone seedlings. In addition, such effect is remarkable in particular with respect to a plant species having low rooting ability. Accordingly, the present invention contributes to quick mass-production of widely various plant species of clone seedlings. In particular, even for a plant species having low rooting ability, the present invention can quickly mass-produce a clone seedling of such a plant species so that the plant species having low rooting ability becomes industrially applicable.

Problems solved by technology

A step of mass-producing plant bodies (seedlings) having a trait appropriate for a purpose and being homogeneous in quality is unavoidably required in industrially using the plant bodies for the purpose of agricultural production, afforestation, breeding, etc.

In particular, if a plant species is low in rooting ability, the low rooting ability becomes a serious hindrance against industrial application of clone seedlings of the plant species.

This method can reduce a mortality rate of the plant tissue to some extent, however, rooting of the plant tissue is suppressed or delayed.

However, rooting rates of some plant species and some particular eucalyptus lines with useful trait cannot be improved sufficiently by these improvements, thereby resulting in low rooting rates that lead to low productive efficiency of seedlings.

This has been a big problem for this technique.

As described above, there has been no technique for obtaining practically enough quantity of clone seedlings from shoots; and for glutathione, it has been merely reported that glutathione influences formation of adventitious embryos of calluses of limited kinds of plant species.