5 results about "Plastid" patented technology

The invention discloses a germplasm aquaporin OsPIP2 and a preparation method thereof. 1 is applied to regulation and control of rice chalkiness, and belongs to the technical field of biological breeding. According to the invention, a gene OsPIP2 for controlling chalkiness of rice is cloned; 1, the nucleotide sequence of the gene is as shown in SEQ ID NO. 1. According to the invention, a gene editing technology Crispr / cas9 is used for modifying OsPIP2 in wild type ZH11; after the function of the rice chalkiness gene OsPIP2 is destroyed, knockout strains show that the chalkiness rate and the chalkiness degree of rice grains are remarkably improved, which indicates that the gene has the function of regulating and controlling rice chalkiness, and meanwhile, analysis finds that the rice chalkiness gene OsPIP2 is a japonica rice type OsPIP2; the chalkiness of 1Hap3 is obviously lower than that of other haplotypes, which indicates that OsPIP2; the 1Hap3 has the potential of reducing chalkiness of indica rice, gene resources can be provided for high-quality breeding of rice, and reference is provided for functional research of other homologous genes.

This invention discloses a method for converting a carbon source into glycolic acid. The method includes: providing a plastid comprising the gene sequences of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3; transfecting the plastid into a bacterial strain to obtain a modified bacterial strain; and providing the carbon source to the modified bacterial strain, enabling the modified bacterial strain to convert the carbon source into glycolic acid. The method disclosed in this invention can improve the yield of glycolic acid production using a fully biosynthetic approach.

The invention discloses a method for improving the fermentation level of microalgae astaxanthin through plant hormone pretreatment, and belongs to the technical field of microalgae biology. According to the method, firstly, under the dark condition, two plant hormones including brassinolide and cytokinin are utilized to conduct pretreatment on a Chromococcus zoffii seed solution so as to increase the number of plastids, and then through shake-flask culture and fermentation tank fed-batch fermentation, it is proved that the strategy can promote accumulation of astaxanthin of Chromococcus zoffii under the nitrogen deficiency condition. The appropriate concentrations of the two plant hormones are both low, high light stress is not needed, the step operation is simple and convenient, and the production cost is reduced.

The invention relates to a transgenic breeding method capable of simultaneously improving carotenoid content, stability and bioavailability of rice endosperm. Expression cassettes of NeuroAL1, NeuroAL2, NeuroAL3 and arabidopsis thaliana tHMGR are constructed by using a rice endosperm specific promoter, all the genes are not positioned in a plastid, and after rice is transformed, transgenic rice with red seed endosperm and rich provitamin A carotenoid and fungus specific carotenoid is obtained, or the NeuroAL1 is replaced by UstiAL1, the seed endosperm color is changed into orange yellow, and the transgenic rice with rich provitamin A carotenoid and fungus specific carotenoid is transformed into fungus specific carotenoid and rich provitamin A carotenoid and fungus specific carotenoid. Compared with the traditional golden rice, the golden rice has the advantages that more provitamin A carotenoids are accumulated, and compared with the traditional golden rice, the content of the provitamin A carotenoids in the endosperm of the golden rice is higher, the light stability is higher, and the bioavailability is also higher, so that a new generation of golden rice with richer nutrition is created on the basis of a new strategy of cytoplasm de novo synthesis.

The present invention relates to polynucleotides for the generation of genetically modified plants, algae or plastids thereof that are capable of producing cannabinoids. In an aspect, the present invention also relates to methods of producing cannabinoids.