3results about How to "Optimize the reaction system" patented technology

The invention belongs to the technical field of animal epidemic disease detection, and provides a specific primer and a probe for fluorescent quantitative PCR (Polymerase Chain Reaction) detection of bacillus anthracis, and the specific primer and the probe comprise primers SEQ ID NO: 1-SEQ ID NO: 4 and probes SEQ ID NO: 5-SEQ ID NO: 6 on plasmids of bacillus anthracis pX01 and pX02. The invention also provides a detection kit containing the 2 * reaction buffer. The kit disclosed by the invention can be used for detecting the bacillus anthracis in the animal skin with high sensitivity and high specificity.

The application discloses a method for preparing L-serine by enzyme catalysis, and in-situ preparation of low-cost and high-activity THF is achieved by using a biological method or a chemical method; THF and enzyme inactivation are avoided by precisely controlling the flow addition of formaldehyde; the reaction system is optimized to promote the in-vivo circulation regeneration of THF, so that higher conversion efficiency is achieved with less THF feeding amount, and the use amount and cost of the expensive coenzyme THF are significantly reduced. The method is based on the optimization of the existing enzyme process, does not need complex equipment modification, and is easy to realize industrialized scale production.

This invention discloses a Is PETase mutants and their application in the degradation of PET plastics. This invention addresses existing... Is The PETase mutant cannot tolerate high substrate (PET plastic) concentrations, thus providing a method to improve the degradation activity of PET plastic. Is PETase mutant. For the aforementioned Is The present invention also optimizes the reaction system of the PETase mutant, thereby providing a method for depolymerizing PET plastics. Using the mutants described in this invention, such as M16-G, the degradation of high-concentration (20%) PET plastics can be completed at a Tg temperature, and it can be used for the degradation and recycling of PET plastics under industrial conditions. Furthermore, the present invention... Is PETase mutants, such as M16-G, can tolerate seawater. Seawater can be used to prepare the reaction buffer required for its degradation of PET plastics, which helps to save freshwater resources and protect the environment.