Bdellovibrio strain for preventing and treating mastitis and applications thereof
A technology for leech vibrio and mastitis, which is applied in the field of leech vibrio strains for preventing and treating mastitis, can solve the problems of consumption of disinfectants, polluted milk, consumption and the like, and achieves the effects of good safety, remarkable effect and wide application range.
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[0040] (1) Isolation and purification of Bdellovibrio BDS01
[0041] Inoculate the host bacteria Escherichia coli (Escherichia coli, purchased from Guangdong Microbial Culture Collection Center, No. GIM 1.137) into 100 mL of sterile LB ((Luria-Bertani)) liquid medium (10 g of peptone, 5 g of yeast extract, 1000 mL of distilled water , pH 7.2) in an Erlenmeyer flask, placed in a constant temperature shaker with a rotation speed of 200 rpm and a temperature of 28° C. for 14 hours, so that it was in the logarithmic growth phase. The culture solution was centrifuged at 6000rpm for 20min at 4°C, the supernatant was discarded, and the precipitated cells were mixed with 1mL DNB (dilute nutrient broth) liquid medium (0.8g nutrient broth, 0.5g casein acid hydrolyzate g, yeast extract 0.1g, dissolved in 1000ml distilled water, pH value is 7.2~7.6) after resuspended (concentration is about 3×10 12 cfu / mL) in a refrigerator at 4°C until use.
[0042] Pretreat the collected soil, take ab...
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