Bdellovibrio strain for preventing and treating mastitis and applications thereof

A technology for leech vibrio and mastitis, which is applied in the field of leech vibrio strains for preventing and treating mastitis, can solve the problems of consumption of disinfectants, polluted milk, consumption and the like, and achieves the effects of good safety, remarkable effect and wide application range.

Inactive Publication Date: 2010-02-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these conventional disinfectants are in direct contact with organic matter such as cow dung and feed residues, causing the disinfectant to be consumed by a large amount of organic matter, hindering the drug's effect, greatly reducing the killing effect of the drug on pathogenic mi

Method used

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  • Bdellovibrio strain for preventing and treating mastitis and applications thereof
  • Bdellovibrio strain for preventing and treating mastitis and applications thereof
  • Bdellovibrio strain for preventing and treating mastitis and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] (1) Isolation and purification of Bdellovibrio BDS01

[0041] Inoculate the host bacteria Escherichia coli (Escherichia coli, purchased from Guangdong Microbial Culture Collection Center, No. GIM 1.137) into 100 mL of sterile LB ((Luria-Bertani)) liquid medium (10 g of peptone, 5 g of yeast extract, 1000 mL of distilled water , pH 7.2) in an Erlenmeyer flask, placed in a constant temperature shaker with a rotation speed of 200 rpm and a temperature of 28° C. for 14 hours, so that it was in the logarithmic growth phase. The culture solution was centrifuged at 6000rpm for 20min at 4°C, the supernatant was discarded, and the precipitated cells were mixed with 1mL DNB (dilute nutrient broth) liquid medium (0.8g nutrient broth, 0.5g casein acid hydrolyzate g, yeast extract 0.1g, dissolved in 1000ml distilled water, pH value is 7.2~7.6) after resuspended (concentration is about 3×10 12 cfu / mL) in a refrigerator at 4°C until use.

[0042] Pretreat the collected soil, take ab...

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Abstract

The invention discloses a Bdellovibrio strain for preventing and treating mastitis and applications thereof. A Bdellovibrio BDS01 is obtained by separation, and is unicellular, arced and 0.9-0.25 micrometer in size; the end of the Bdellovibrio BDS01 has flagellum which is 4 micrometers long. When cultured by a two-layer plating method at 28 DEG C for four days, the Bdellovibrio BDS01 can form transparent round plaques with the diameter of 1-2 mm. The microbial preparation prepared by using the Bdellovibrio BDS01 plastid has favorable effects for preventing cow mastitis. Compared with the telotroch, the plastid has the characteristics of convenient preparation and storage, strong environmental resistance, slightly slow sterilization action, longer sterilization action time, and the like, and does not have toxic or side effect on cows. Therefore, the microbial preparation containing the Bdellovibrio BDS01 plastid is suitable for large-size factory culture of cows, and a novel method forgreen processing and production of milk and for preventing and treating mastitis can be provided.

Description

technical field [0001] The invention relates to a Bdellovibrio, in particular to a Bdellovibrio strain for preventing and treating mastitis and application thereof. Background technique [0002] Cow mastitis (also called mastitis) is one of the three most common diseases of dairy cows. It not only seriously affects the milk production and milk quality of dairy cows, but also endangers human health. The annual loss caused by recessive mastitis in my country is about 135 million yuan, and in the world, the annual loss is estimated to reach 3.8 million tons of milk. The incidence of mastitis in my country is higher than the world average, including recessive mastitis, the average incidence is 70%. It can be seen that cow mastitis is very harmful to the dairy industry. [0003] Pathogen infection is the main cause of mastitis, including bacteria, fungi, viruses, mycoplasma, etc. The main pathogenic bacteria are bacteria, the common ones are Staphylococcus aureus, Escherichia ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P15/14A01N63/02A01P1/00A23K1/18A23L1/015C02F3/34C12R1/01A23K10/18A23K50/10A23L5/20
Inventor 蔡俊鹏鲁锋蒋小平
Owner SOUTH CHINA UNIV OF TECH
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