114 results about "Mastitis in dairy cattle" patented technology

The invention discloses a Bdellovibrio strain for preventing and treating mastitis and applications thereof. A Bdellovibrio BDS01 is obtained by separation, and is unicellular, arced and 0.9-0.25 micrometer in size; the end of the Bdellovibrio BDS01 has flagellum which is 4 micrometers long. When cultured by a two-layer plating method at 28 DEG C for four days, the Bdellovibrio BDS01 can form transparent round plaques with the diameter of 1-2 mm. The microbial preparation prepared by using the Bdellovibrio BDS01 plastid has favorable effects for preventing cow mastitis. Compared with the telotroch, the plastid has the characteristics of convenient preparation and storage, strong environmental resistance, slightly slow sterilization action, longer sterilization action time, and the like, and does not have toxic or side effect on cows. Therefore, the microbial preparation containing the Bdellovibrio BDS01 plastid is suitable for large-size factory culture of cows, and a novel method forgreen processing and production of milk and for preventing and treating mastitis can be provided.

The invention discloses application of a cordyceps strain fermentation product to reduce somatic cell count in cow's milk, discloses application of the cordyceps strain fermentation product to improve cow milk yield and prepare forages for preventing cow mastitis. The application comprises that the cordyceps strain fermentation product is used to feed lactation cows. The cordyceps strain fermentation product is obtained by performing liquid fermentation or solid fermentation on a cordyceps fungus. Compared with a contrast group, a forage containing a cordyceps forage additive and used to feed lactation cows is capable of substantially improving cow milk yield, the produced cow's milk has substantially improved fat content and protein content and substantially reduced somatic cell count, and after feeding is stopped, the somatic cell count in the produced cow's milk still keeps a relatively low level. The cordyceps forage additive is shown to be capable of effectively change the health status of cows and improve the quality of the produced cow's milk, and is capable of effectively preventing cow mastitis, reducing somatic cell count in cow's milk and keeping the somatic cell count at a relatively low level for a long time.

The invention discloses a medicated bath liniment for cow nipples for preventing cow mastitis, and a preparation method of the medicated bath liniment. The medicated bath liniment is prepared from more than ten raw materials such as a film-forming agent. The medicated bath liniment has the advantages on the following three aspects: 1, the film-forming agent is added to medicated bath liquor, so that a protective screen is formed between each nipple and the environment; 2 a skin care agent is added to the medicated bath liquor, so as to protect the skin of the nipples; and 3 sterilization, film formation and skin care are organically combined, so that the functions of a medicated bath disinfector for the nipples are achieved, the nipples are protected in an omnibearing manner, and the cow mastitis is very effectively prevented.

The invention belongs to the technical field of feed additives, and particularly relates to a feed additive for improving immunity of cows in the milk production period by use of perilla and a preparation method of the feed additive. The feed additive comprises perilla antibacterial peptide, fermented alfalfa meal, a traditional Chinese medicine extract preparation and a complex enzyme preparation. The perilla antibacterial peptide is prepared from perilla seed meal left after oil pressing of perilla seeds. The perilla antibacterial peptide acts together with perilla leaf, dandelion, honeysuckle flower, Shuanghuanglian, fructus forsythiae and isatis root extracts with antibacterial and anti-inflammatory effects in the feed additive and can effectively treat and prevent mastitis of the cowsand improve the immunity of the cows in the milk production period. The fermented alfalfa meal, the traditional Chinese medicine extract preparation and the complex enzyme preparation in the feed additive provide a richer and more balanced nutrient basis for the cows and can effectively promote milk secretion and improve the yield and quality of milk, and green milk without hormones, antibioticsand chemical residues can be obtained.

The invention discloses a Chinese traditional medicine powder medicine for treating milk cow mastitis which is prepared from capsule of weeping forsythia, dandelion, elephantopus, Bupleurum root, baikal skullcap root, white mustard seed, dragon's bone, oyster shell, motherwort, frankincense, and myrrh.

The invention discloses a medicinal composition for enhancing immunity of cow and controlling mastitis, and relates to a medicament capable of enhancing the immunity of livestock and controlling diseases of the livestock, particularly breast diseases of the livestock. The medicinal composition comprises the following components based on every 100 weight parts: 2 to 6 parts of levamisole hydrochloride, 2 to 6 parts of water soluble vitamin E, 43 to 53 parts of astragalus polysaccharides, and 43 to 53 parts of epimedium herb. During preparation, the epimedium herb is crushed and screened, so that the granularity reaches 60 to 200 meshes; the epimedium herb is uniformly mixed with the levamisole hydrochloride, the water soluble vitamin E, and the astragalus polysaccharides; and the mixture is separately packaged, and the finished product is obtained. The medicinal composition for enhancing the immunity of cow and controlling mastitis has simple preparation method and low cost, and can fully utilize domestic rich Chinese medicament resources. The product can effectively prevent and control the cow mastitis, enhance the immunity of cow, has no side effect, cannot produce medicament resistance, particularly has no residue in the cow body after administration, and can further effectively solve the problem of milk drinking safety.

The invention provides a traditional Chinese medicine composite for treating the mastitis of dairy cattle. The traditional Chinese medicine composite is characterized by comprising the following components in percentage by weight: 20-40% of dahurian patrinia herb, 20-40% of arrowshaped tinospora root, 10-20% of sedum lineare, 10-20% of pipewort, 10-20% of hematoxylon, 10-20% of cortex erythrinae, 5-10% of concha arcae, 5-10% of eupatorium, 5-10% of inula japonica thunb, 1-5% of tribulus terrestris and 1-5% of pericarpium arecae. The composite provided by the invention is added with conventional auxiliary materials to be prepared into powder, oral liquid, breast perfusion and injection which are acceptable in clinic through the conventional process in pharmacy.

The invention provides a dairy cow early-stage mastitis monitoring method. The method includes following steps: (1), performing SCC count detection on milk; (2), performing pathogenic microorganism identification of the milk: colony count and bacteriological identification; (3), performing somatic cell typing detection; (4), identifying protein differential expression level and function of the milk: protein concentration measuring, protein quantifying and analysis protein differential expression level and function identification. By detecting differentiation degree of immune cells in cow's milk somatic cells and identifying expression level and function of mycoprotein in the milk, the method is conducive to timely finding early-stage mastitis and pathogen types inducing early-stage inflammation to make full preparation for timely prevention and targeted treatment of dairy cow mastitis.

The invention discloses an experimental method for an application of niacin in treating cow mastitis by using a GPR109A receptor. The experimental method comprises the steps of: S1, selecting cows with mastitis, wherein cows with positive mastitis are selected preliminarily, and a number of somatic cells of each cow is about 80-200 million / mL; S2, and detecting pro-inflammatory factors in cow serum and whey, wherein peripheral blood in normal cows, the cows with mastitis and niacin-fed cows with mastitis are collected on day 0 and day 7 respectively, and serum in the blood is extracted. The experimental method plans a whole new treatment strategy for cow mastitis, detects the expression of pro-inflammatory mediators in the cows with mastitis after niacin treatment by separating the serum and whey of the cows, comprehensively evaluates the therapeutic effect of niacin in treating cow mastitis by adopting the molecular biological method, and can achieve the effects of protecting mammaryglands and relieving inflammation since niacin can effectively reduce the expression of pro-inflammatory mediators in cow serum and whey.

A method for identifying mastitis in a dairy animal, the method including the steps of: (i) identifying a dairy animal suspected of being affected by mastitis; (ii) collecting a sample of milk from the identified dairy animal; (iii) analyzing the sample for the presence of one or more mastitic bacteria; (iv) analyzing the sample for the presence of one or more mastitic pathogens; (v) identifying, if the presence of one or more mastitic bacteria is indicated, the mastitic bacteria in the sample; and (vi) determining, based on the identified mastitic bacteria in the sample, a management decision for the dairy animal.

The invention provides a traditional Chinese medicine composite with an immune function and used for treating mastitis of cows. The traditional Chinese medicine composite contains the following crude medicines: sweetgum fruits, liquorice, rhizoma polygonati, herba violae, Chinese pulsatilla roots, rhizoma panacis majoris, eucommia bark, folium isatidis, myrrh, cortex moutan, folium eriobotryae, coptis roots, multiflower knotweed vines, white paeony roots, turmeric, radix astragali, rhizoma atractylodis macrocephalae, fructus choerospondiatis, schisandra chinensis and radix glehniae. The traditional Chinese medicine composite is capable of improving the immunity of the cows simultaneously when clearing heat, detoxifying, and anti-bacterially decreasing inflammation, thereby improving the anti-stress and the anti-disease capability of the cows; and the traditional Chinese medicine composite has the advantages of good treatment effect, reoccurrence prevention, no medicine resistance, no residue, and no toxic and side effects in treating mastitis of the cows.

Disclosed is preparation of a cocktail as a therapeutic agent for cow mastitis and use thereof. A compatibility of phages against the main pathogenic bacteria of cow mastitis is utilized in combination with a specific preparation method to prepare and obtain a therapeutic cocktail preparation. The therapeutic phage cocktail preparation for cow mastitis provided in the disclosure is easy to prepare, short in course of treatment and quick in effect. It has better therapeutic effect on both clinical cow mastitis and recessive mastitis than that of antibiotics and antimicrobial peptide and is expected to solve the problem of bacterial resistance in the treatment of cow mastitis, thereby reducing or getting rid of the troubles of diseases such as mastitis in cows, which is of potential development value in cow farming.

The invention provides a core promoter for influencing cow mastitis infectibility / resistance HMGB3 gene and a functional molecule mark and an application, which belongs to the biochemistry genetic breeding technical field. The concrete method is characterized in that a cow mastitis infectibility / resistance HMGB3 candidate gene is provided through inverse transcription RT-PCR and real time fluorescence quantification RT-qPCR, a functional SNP site information of a cow HMGB3 gene P3 promoter zone is used, a molecular biology correlation technology is used for discriminating the genotype of the cow at this site, and the genotype having mastitis resistance is selected. The favorable genotype is selected for keeping species, the frequency of the HMGB3 gene resistance genotype in a cow colony can be enhanced, thereby the capability of anti-mastitis by the cow colony is enhanced, the incidence of disease of the cow mastitis is reduced, and the economy loss is reduced. The invention provides the novel method for mastitis resistance genetic improvement.

The invention relates to staphylococcus aureus FnBPA-A protein mimic epitope peptides having immunizing protection, a mimic epitope peptide composition, and applications of the mimic epitope peptides and the mimic epitope peptide composition. Two immunoprotective mimic epitope peptides provided by the invention have the amino acid sequences respectively shown in SEQ ID NO:1 and SEQ ID NO:2, the mimic epitope peptide composition consists of two polypeptides shown in SEQ ID NO:1 and SEQ ID NO:2, and the mass ratio of the polypeptide shown in SEQ ID NO:1 to the polypeptide shown in SEQ ID NO:2 is 2 to 1. Experiment animal immunoprotective tests show that the two mimic epitope peptides can stimulate a body to produce high-level specific antibodies and have a certain degree of immunizing protection; moreover, the mimic epitope peptide composition has the immunoprotective effect on staphylococcus aureus infection better than that of an FnBPA-A holoprotein. Therefore, the mimic epitope peptides and the composition thereof can be used as effective components for development of multi-epitope vaccines of staphylococcus aureus and prevention of cow mastitis caused by staphylococcus aureus.

The invention aims to provide a drug delivery system with bovine antimicrobial peptide BMAP28 as therapeutic gene, a gene expression vector, a drug delivery vector and a preparation method and application thereof. The molecular cloning technique containing PCR technology, point mutation technology and enzyme digestion and connection, etc. is utilized, and a BMAP28 gene-carrying secretion-type eukaryotic expression plasmid is constructed. Naked plasmid DNA is encapsulated with chitosan nanoparticles to prepare the antimicrobial peptide gene-loaded drug delivery system. The drug delivery system of the invention can effectively mediate a gene drug to be expressed in eukaryotic cell and secreted to ectoenzyme. The system has good curative effects on cow mastitis.

The invention discloses a recombinant plasmid of a GSDMD-N (gasdmermin D-N) gene, an expression method in mammary gland and an application, and belongs to the field of genetic engineering. An expression recombinant plasmid of the GSDMD-N gene is constructed and enabled to be specifically expressed in breast tissue and breast cells, so that mastitis caused by staphylococcus aureus infection is prevented and treated. GSDMD-N protein is a polypeptide compound found in recent years, which can specifically recognize biofilm component cardiolipin of bacteria and can be combined with cardiolipin to form a plurality of honeycomb-shaped pores, so that bacteria are disintegrated and die. According to the technology, the exogenous gene GSDMD-N is efficiently expressed in animal mammary glands througha specific promoter WAP (whey acidic protein) of breast tissue by a mammary bioreactor, staphylococcus aureus causing cow mastitis is killed, and accordingly, the cow mastitis caused by staphylococcus aureus infection is prevented and treated. The recombinant plasmid and the recombinant attenuated salmonella have the advantages of being efficient, safe, low in cost and the like, provide a a new approach for prevention and control of the cow mastitis caused by staphylococcus aureus infection, and have good application prospect.

The invention relates to a Chinese medicinal composition for controlling cow mastitis, which is characterized by consisting of the following medicaments in portion by weight: 23 to 27 portions of dandelion, 23 to 27 portions of honeysuckle flowers, 11 to 15 portions of wild chrysanthemums, 11 to 15 portions of angelica, 11 to 15 portions of reed rhizome, 3 to 7 portions of gardenia, and 6 to 10 portions of scutellaria. A preparation method comprises the steps of weighing dandelion, honeysuckle flowers, wild chrysanthemums, angelica, reed rhizome, gardenia and scutellaria as raw materials in portion by weight, grinding, sifting, allowing particle size to reach 60 to 200 meshes, evenly mixing, separately packing and obtaining finished products. The invention has the advantages that as bacterial infection is a main cause of cow mastitis and the incidence of the cow mastitis is often accompanied by different degrees of inflammation and hepatic depression, the composition can clear heat, release toxin, resist inflammation, reduce swelling, regulate Qi, activate collaterals so as to ensure smooth Qi blood, stop pain, reduce swelling, resolve and soften hard lumps. The composition is prominent in efficacy, high in therapeutic effects, free from drug residues as well as toxic side effects, particularly overcomes the problem of residues of the prior antibiotics and chemical drugs in dairy products, and gives play to the effects of Chinese herbal medicament in resisting bacteria, diminishing inflammation, promoting blood circulation and removing blood stasis during disease treatment.

The invention relates to a traditional Chinese medicinal composition for treating dairy cow mastitis. The composition is prepared by 13 types of dry raw materials of traditional Chinese medicine including Mongolian dandelion herb, herba violae, folium isatidis, peppermint, rhubarb, rhizoma dioscoreae bulbiferae, radix trichosanthis, flos chrysanthemi indici, baical skullcap root, golden thread, lightyellow sophora root, tangerine peel, and honeysuckle flower. The invention also relates to a preparation method of the composition and applications thereof used for preparing medicines for treating dairy cow mastitis; the products of the invention have obvious therapeutic action to dairy cow mastitis, especially intractable mastitis diseases.

The invention discloses a golden staphylococcus adhesin functional peptide for milk cow mastitis, a polynucleotide for coding adhesin protein and a biological method for preparing adhesin protein function by the genetic engineering; simulantesouly, the invention discloses the possible uses of the golden staphylococcus adhesin functional peptide in related field. The invention has the advantages of helping to further research the pathogenesis of the mastitis caused by the golden staphylococcus and the corresponding vaccine.

The invention belongs to the technical field of medicine, in particular relating to a freeze-dried powder preparation for curing bovine mastitis. The preparation disclosed in the invention comprises the following components by weigh percent: 0.04-1.5wt% of lysostaphin, 10-72.5wt% of lysozyme, 10-40wt% of retardant, 5-20% of monopotassium phosphate and 10-35% of disodium hydrogen phosphate. When the preparation is poured into the bovine breast, the lysostaphin and lysozyme can maintain favorable stability and sterilization vigor in milk, and flocculent precipitates generated by polymerization of lysostaphin or lysozyme with casein never appear in milk.

The invention discloses a pair of primers for screening cows with mastitis resistance. The sequence of an upstream primer is shown in SEQ ID NO.7 and the sequence of a downstream primer is shown in SEQ ID NO.8. The invention also discloses a kit for screening cows with mastitis resistance. The kit comprises the primers and conventional reagents required for PCR (polymerase chain reaction) amplification. The primers and the kit can be used for screening cows with mastitis resistance. An application method comprises the steps of extracting genome DNA (deoxyribonucleic acid) of cows to be detected, carrying out specific PCR amplification by utilizing the primers, and detecting the PCR amplification products to judge the genotype, wherein the hybrid type has relatively strong mastitis resistance, therefore, the cow individuals with hybrid genotypes are selected for genetic molecular breeding of cows. The method has the effects of improving the accuracy of selection of cow individuals with mastitis resistance and saving the genetic improvement time of mastitis resistance of cows, and has good application prospects.

The invention discloses a traditional Chinese medicine composition for treating dairy cow mastitis. The traditional Chinese medicine composition is a medicament prepared from the following raw materials expressed in weight parts: 5 to 8 parts of green tangerine peel, 5 to 8 parts of liquorice root and 8 to 15 parts of dandelion. The traditional Chinese medicine composition provided by the invention has the effects of clearing heat, removing toxins, activating blood circulation to expel blood stasis and resolve static blood, removing edema and promoting lactation and can overcome defects like milk pollution and poor quality of dairy products caused by antibiotic treatment. According to results of clinical diagnosis and treatment, the traditional Chinese medicine composition has a remarkable treatment effect, and milk yield of a dairy cow quickly returns to normal after the dairy cow is healed.

The invention relates to a cow mastitis key SNPs locus rs88640083 and 2b-RAD genotyping and analysis method. The method comprises the following steps: constructing a library and sequencing; Bioinformatics analysis: data filtering, digestion sequence extraction, data alignment, SNP typing, genome-wide association analysis; and performing genome-wide association analysis (GWAS) of clinical mastitisphenotypic traits in dairy cows by BayesA model and Logistic regression model. Compared with the prior art, the present invention has the advantages that compared with RADseq, 2b-RAD sequencing has the following advantages: 1. The length of enzyme fragments is uniform and no subsequent screening is needed; 2, that enzyme section does not need to add a Y-shaped link; 3, the steps are simple; 4, thesequence cost of each sample is low; 5, the sequence takes short time. The invention also constructs two genome-wide association analysis models (BayesA and Logistics); and a Chinese Holstein mastitis key SNPs locus and its corresponding gene (SYK) are screened.

The invention provides a preparation method of a Dicranostigma leptopodum extract perfusate. The method comprises the following steps: adding water to a Dicranostigma leptopodum extract, decocting, mixing filtrates, and concentrating to obtain a concentrate; and extracting with ethanol, removing ethanol, adding benzyl alcohol and Tween-80 to the above obtained ethanol removed medicinal liquid, adding injection water to a full dose, and sterilizing to obtain the Dicranostigma leptopodum extract perfusate. The invention also provides an application of the perfusate in the preparation of medicines for treating cow mastitis. The Dicranostigma leptopodum extract perfusate adopting the Dicranostigma leptopodum extract as an effective component has very good heat clearing, detoxifying, bacterium resisting, inflammation eliminating and virus resisting effects. The perfusate is a pure traditional Chinese medicinal preparation, contains no chemical components, and is in favor of the safety of dairy products. Products produced through extraction and preparation integrated production have the advantages of stable properties and controllable quality, and are in favor of industrial production. The perfusate has the advantages of definite effect, no residual or toxic side effects, and realization of use in the lactation period as an effective medicine for treating clinic mastitis of cows, and becomes a good substitute of antibiotics.

The invention discloses a preparation method of a compound Chinese herb and an extract thereof for treating bovine mastitis, belonging to the technical field of veterinary drugs. The compound Chinese herb for treating bovine mastitis is prepared from the following components in parts by weight: 2-6 parts of dandelion, 2-6 parts of rattletop, 2-6 parts of selfheal, 2-4 parts of rheum officinale, 1-4 parts of radix bupleuri, and 1-4 parts of analgesic-antipyretic medicine. The compound Chinese herb is high in curative effect, safe and reliable, non-toxic and pollution-free, and free of residue, can be applied to the field of prevention and treatment for the dairy cattle breeding industry and the milch goat breeding industry, and is an efficient product for preventing and treating mammitis; after an analgesic-antipyretic drug is added into the compound Chinese herb, the effect is more obvious, and the time for taking effect is shorter, and the cure rate is high; the preparation method is simple in technology, low in cost, and storing in practicability.

The invention discloses a staphylococcus aureus inactivated vaccine for bovine mastitis and a preparation method thereof. The method comprises the steps of firstly isolating and identifying a sample of bovine mastitis milk juice, screening one strain of 5, 8, and 336 type staphylococcus aureus with good immunogenicity and stable passage, and performing steps including fermental cultivation, inactivation and the like to obtain an antigen stock solution of inactivated thallus; at the same time, constructing an SEB protein expression strain and an Hlalpha protein expression strain, and performingsteps including induction culture, centrifugation, purification, inactivation and the like to obtain an antigen stock solution of recombinant protein SEB and Hlalpha; and finally, mixing the antigenstock solutions with adjuvants to prepare the staphylococcus aureus inactivation vaccine for bovine mastitis. The staphylococcus aureus inactivation vaccine for bovine mastitis has good safety and caneffectively prevent infection of the 5, 8, and 336 type staphylococcus aureus. The staphylococcus aureus inactivation vaccine for bovine mastitis is of great significance to prevent and control popularity and spreading of bovine mastitis.

The invention refers to an anti-cow mastitis pathogenicbacteria compound yolk antibody (anti-MasIgY), the making method and its application of making drugs curing relative diseases caused by infection of cow mastitis or feed additive. Test anti-MasIgY according to SDS-PAGE electrophoresis (7.5% separating glue), its chart showing single band; colorize blue by R250; use immune mark Western-blotting to test the clear peculiar ferment-dyed stripes appearing in medium-high molecular weight banjd; adopts the biochemical method of double-contracted urea to determine protein concentration. The method: making the cow mastitis pathogenicbacteria antigen, make immunity on healthy hens by the antigen, collecting eggs, and then extracting anti-MasIgY from the eggs.

The invention discloses a medicine for treating mastitis in dairy cattle. The medicine is prepared from radix astragali, taraxacum and prodigiosin. The medicine is effective for mastitis in dairy cattle, including acute mastitis in dairy cattle, chronic mastitis in dairy cattle, suppurative mastitis in dairy cattle and the like. The effective rate can reach 98.7%, and the cure rate is 93.7% or so. The medicine disclosed by the invention has high cure rate for mastitis in dairy cattle, saves raw materials, and thus, is an ideal medicine for treating mastitis in dairy cattle.

The invention discloses a compound preparation for treating dairy cow mammitis. The compound preparation is prepared from, by weight, 1 part of lavender essential oil, 1-2 parts of cinnamon essential oil, 1-3 parts of wild marjoram essential oil and 3-6 parts of auxiliary materials. A preparation method of the compound preparation is provided. The compound preparation and the preparation method have the advantages that the plant essential oil is adopted as effective ingredients, the good effects of clearing away heat and toxic materials, resisting bacteria and diminishing inflammation are achieved, the effect of treating dairy cow mammitis is extremely good, and due to the adoption of the pure traditional Chinese medicine preparation and no chemical drug ingredient is contained, the safety of dairy products is facilitated. Uniform solids are obtained through stirring, stable performance and controllable quality are achieved, and industrial production is well facilitated. The compound preparation serves as traditional Chinese medicine, the effect of the compound preparation is definite, no residue or toxic or side effect is generated, the compound preparation can be used in a lactation period and can become good substitute goods of antibiotics, and the obtained traditional Chinese medicine compound ointment has high application value in veterinary clinical medicine.