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Freeze-dried powder preparation for curing bovine mastitis

A technology for mastitis and freeze-dried powder, which is applied in freeze-dried transportation, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., can solve the problems of reduced bactericidal activity of lysostaphylococcus enzyme, affecting milk quality, etc. , to achieve a significant therapeutic effect

Inactive Publication Date: 2011-04-13
SHANGHAI HI TECH UNITED BIO TECHCAL RES +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in practical application, we found that due to the presence of a large amount of casein in milk, its isoelectric point is about 4.6, while the isoelectric point of lysostaphin is about 10.5-11.0, and a large dose of lysostaphin is perfused into After the mammary gland tissue of dairy cows, it will react with casein in milk to form flocculent precipitates, which will reduce the bactericidal activity of lysostaphin and affect the quality of milk

Method used

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  • Freeze-dried powder preparation for curing bovine mastitis
  • Freeze-dried powder preparation for curing bovine mastitis
  • Freeze-dried powder preparation for curing bovine mastitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Formula of recombinant lysostaphin freeze-dried powder preparation

[0019] Recipe 1:

[0020] Lysostaphin 0.04% (0.1mg), lysozyme content 72.5% (181.25mg), acacia gum 10.87% (27.175mg), potassium dihydrogen phosphate 5.76% (14.4mg), disodium hydrogen phosphate 10.83% (27.075mg ).

[0021] Recipe 2:

[0022] Lysostaphin 0.23% (0.23mg), lysozyme content 56.77% (56.77mg), soluble soybean polysaccharide 17.03% (17.03mg), potassium dihydrogen phosphate 9.01% (9.01mg), disodium hydrogen phosphate 16.96% (16.96 mg).

[0023] Recipe 3:

[0024] Lysostaphin content 1.09% (1mg), lysozyme content 10.9% (10mg), polyvinylpyrrolidone 38.14% (34.99mg), potassium dihydrogen phosphate 17.31% (15.88mg), disodium hydrogen phosphate 32.56% (29.87mg ).

[0025] Recipe 4

[0026] Lysostaphin 0.23% (0.23mg), lysozyme content 56.77% (56.77mg), pectin 17.03% (17.03mg), potassium dihydrogen phosphate 9.01% (9.01mg), disodium hydrogen phosphate 16.96% (16.96mg ).

[0027] Recipe 5

...

Embodiment 2

[0034] The screening experiment of embodiment 2 formula

[0035] Prepare 100ml of solution with 0.2mol / L phosphate buffer solution of pH7.0, contain recombinant lysostaphin 2mg (enzyme activity is 2000U), this solution is divided into 20 parts, every part is 5ml. Then add the following auxiliary materials in a weight ratio of 1:1. After mixing evenly, add 20ml of freshly collected normal milk (pasteurized), incubate at 39°C for 20 hours, and then observe the changes in the properties of the milk after adding samples with different serial numbers. Preservation of enzyme bioactivity in milk. The specific results are shown in Table 1 below. The result of table 1 shows: arabic gum, propylene glycol alginate, pectin, soluble soybean polysaccharide, polyvinylpyrrolidone can well stabilize the activity of lysostaphin in milk, so our 5 formulas in embodiment 1 The stabilizers mentioned above were used.

[0036] Table 1 Pre-experimental results of prescription screening

[0037] ...

Embodiment 3

[0039] Example 3 Recombinant lysostaphin freeze-dried powder can effectively kill Staphylococcus aureus in milk

[0040] Refer to the 2002 edition of "Disinfection Technical Specifications". Get 1 bottle of freeze-dried powder prepared in Example 1 and dissolve it with 10ml of fresh milk and finally dilute it into 50ml of milk for subsequent use. Arrange the test tubes on the test tube rack and mark them well, and divide the prepared samples into the test tubes, the filling volume is 5ml / tube. Take the freshly cultured Staphylococcus aureus thallus, dilute it to a concentration of about 108cfu / ml with a diluent of physiological saline, and use it on the same day. Add 100 μl of bacterial solution into the sample tube, mix quickly, and start timing. After acting for 30 minutes and 60 minutes, take 0.5ml of the bacteria-drug mixture into a test tube containing 4.5ml of neutralizing agent, shake and mix well. After acting for 10 minutes, dilute the liquid appropriately, and the...

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Abstract

The invention belongs to the technical field of medicine, in particular relating to a freeze-dried powder preparation for curing bovine mastitis. The preparation disclosed in the invention comprises the following components by weigh percent: 0.04-1.5wt% of lysostaphin, 10-72.5wt% of lysozyme, 10-40wt% of retardant, 5-20% of monopotassium phosphate and 10-35% of disodium hydrogen phosphate. When the preparation is poured into the bovine breast, the lysostaphin and lysozyme can maintain favorable stability and sterilization vigor in milk, and flocculent precipitates generated by polymerization of lysostaphin or lysozyme with casein never appear in milk.

Description

technical field [0001] The invention relates to a pharmaceutical preparation, in particular to a freeze-dried powder preparation for treating cow mastitis. Background technique [0002] Dairy cow mastitis is a common, complex disease that causes the most serious economic losses in the dairy industry. At home and abroad, large doses of antibiotics are often used to treat dairy cow mammary gland infection. Although antibiotics have certain curative effects, most of the above-mentioned pathogenic bacteria have produced drug-resistant strains, so the curative effect is not obvious. On the contrary, it causes antibiotic residues in the milk, and the consequences of mixing the milk produced by these cows into the edible milk are serious. Both the Food and Agriculture Organization of the United Nations and the World Health Organization recommend that the milk expressed within at least 3 days after the withdrawal of antibiotic treatment from cows should not be directly used as raw ...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K38/48A61K47/36A61K47/32A61K47/34A61P15/14A61K47/10
Inventor 黄青山崔福英陆锦春
Owner SHANGHAI HI TECH UNITED BIO TECHCAL RES
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