The invention discloses a preparation method of a pre-prepared active Chinese herbal medicine material-added cow supplement feed. The preparation method comprises the following steps: after uniformly stirring corn, pre-prepared active Chinese herbal medicine materials, barley flour, edible mushroom dregs, wheat bran, peanut vines, apples, canola oil, tofukasu, vinasse, bone meal, fish meal, black bean flour, sodium alginate, skim milk powder, calcium hydrogen phosphate, sodium bicarbonate, salt, fructose syrup, and compound amino acid, drying until the moisture content is 15-18wt%, and then pelletizing to obtain the pre-prepared active Chinese herbal medicine material-added cow supplement feed. The cow supplement feed is added with the natural pollution-free pre-prepared active Chinese herbal medicine materials, so that the cow lactation is greatly promoted, the cow milking quality is improved, and the requirement of the dairy industry for the high-quality supplement feed is met.