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Lactobacillus plantarum CW006 with antihypertensive function

A technology of Lactobacillus plantarum and strains, applied in the food processing and pharmaceutical industries, the field of new strains of Lactobacillus plantarum, can solve the problems of increased blood pressure, loss of diastolic vasoactive activity, increased blood pressure, etc.

Active Publication Date: 2006-10-11
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, in the RAS system, ACE can hydrolyze inactive angiotensin I (AngI) into active angiotensin II (AngII), which promotes the constriction of blood vessels and increases blood pressure; on the other hand, in the KKS system , it can hydrolyze bradykinin (BK), so that the activity of BK to relax blood vessels is lost, which further aggravates the increase of blood pressure

Method used

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  • Lactobacillus plantarum CW006 with antihypertensive function
  • Lactobacillus plantarum CW006 with antihypertensive function
  • Lactobacillus plantarum CW006 with antihypertensive function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preliminary Screening

[0038] Taking traditional fermented food (douche, bean paste, yoghurt, Tibetan mushroom) as samples, lactic acid bacteria were separated, purified and preserved by Gram staining and catalase experiments. After culturing in MRS medium for 24 hours, transfer it to fresh MRS medium, culture under the same conditions for 12 hours, centrifuge the bacteria, resuspend with PBS buffer, add 1% to 10% skimmed milk (SKM), and ferment at 37°C For a certain period of time, the fermented milk was refrigerated and centrifuged at 8500×g at 4°C for 10 minutes, and the obtained supernatant was adjusted to pH 8.3 with 10mol / L NaOH, and stored at -70°C until testing. The method of Cushman and Cheung (1971) was used to measure the activity of inhibiting ACE in fermented milk.

[0039]

[0040] O.D. A is the absorbance value in the absence of inhibitor

[0041] O.D. B is the absorbance value in the presence of inhibitor and ACE

[0042] O.D. C Absor...

Embodiment 2

[0046] The acid and bile resistance characteristics of embodiment 2 lactic acid bacteria

[0047] The experimental strains were suspended in MRS medium with pH 2.0 and pH 3.0 respectively, cultured at 37°C for 3 hours, and the survival rate of viable bacteria was determined by plate counting method.

[0048] The experimental strains were inoculated into MRS and MRSO (MRS with 0.3% ox bile added) at 1% inoculation amount, cultured at 37°C, and the time difference between MRS and MRSO culture solution reaching 0.3 unit growth at A620nm was used as the evaluation of the bile tolerance of the strain in accordance with.

[0049] Strain number

[0050] Strain number

[0051] CW006

Embodiment 3

[0052] Example 3 Effect of Simulated Gastrointestinal Digestion on ACE Inhibitory Activity

[0053] The whey prepared above was subjected to ultrafiltration through a cellulose membrane with a molecular weight cut-off of 10,000 Da (permeate) for determination. According to Sandrine Parrot's method, adjust the whey pH to 2.0 with 1M hydrochloric acid, take 10ml of the sample solution and add 1ml of pepsin solution (0.1g of pepsin dissolved in 1L of 0.01M hydrochloric acid), where E / S is 1:200, at 37 ℃ water bath shaker (160 times / min) digestion reaction for 90min, then heated in 85℃ water bath for 5min to terminate the reaction, adjust the pH of the sample solution to 7.5 with 4% sodium hydroxide, add 0.2ml trypsin solution (0.5g trypsin solution In 1L deionized water), where E / S is about 1:200, reacted for 240min under the same conditions as above, and then heated in a water bath at 85°C for 5min to terminate the reaction. Samples were taken after pepsin digestion for 90min, ...

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Abstract

This invention discloses a new Lactobacillus plantarum strain CW006(conservation number CCTCC M 206032) with an antihypertensive effect, and the hygienic and medical agents related, belonging to the micro-biological additives technological field. This Lactobacillus plantarum strain CW006 has a proteolysis activity and can acquire the antihypertensive effect by producing angiotensin-converting enzyme inhibitor via fermentation of raw milk. With these characteristics, it can be used as starting strain in the dairy industry, and applied in various health care products and medical agents as theraputic additives.

Description

technical field [0001] The present invention relates to a new bacterial strain of Lactobacillus plantarum and the application of the bacterial strain in the production and manufacture of products with antihypertensive (and / or hypotensive) effects, such as in food processing and pharmaceutical industries. It belongs to the technical field of microbial products. technical background [0002] Hypertension is a clinical syndrome characterized by elevated arterial vascular systolic or diastolic blood pressure and is a common cardiovascular disease. It has a high incidence and is often accompanied by hyperlipidemia, coronary heart disease, diabetes, atherosclerosis and other diseases, as well as functional or organic changes in the heart, blood vessels, brain and kidneys, which pose a great threat to health. major global public health problem. [0003] In organisms, many systems are closely related to the maintenance of blood pressure, among which the central nervous system, adr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K9/19A61K9/48A61K35/747A61P9/12C12R1/25
Inventor 陈卫张灏田丰伟赵建新商靓靓黄文利王宇
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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