598 results about "Proteolysis" patented technology

The present invention relates to various molecular constructs having at least one cellular-targeting element, one protein-targeting element, and one ubiquitin ligase recruitment element, wherein these elements are conjugated in a manner that facilitates cellular uptake and targeted degradation of specific proteins within cells. The present invention further relates to multifunctional proteolysis-targeting chimera conjugates and uses thereof for the treatment of diseases or disorders such as immune and inflammatory diseases, infectious diseases, cardiovascular diseases, endocrine disorders, and various cancers that are otherwise resistant to traditional therapeutic regimens.

The invention provides a tripeptide for promoting bone growth, the tripeptide is VPF, the amino acid sequence of the tripeptide is Val-Pro-Phe, the molecular weight of the tripeptide is 361.44 Da, and the tripeptide belongs to the technical field of functional bioactive peptides. The tripeptide can be prepared from egg yolk protein through enzymolysis separation and purification, can also be obtained through chemical solid-phase synthesis, has a good effect of promoting bone growth, can remarkably improve the growth and proliferation level of osteoblasts in a body, promotes synthesis and secretion of bone matrixes, and has a good application prospect. Meanwhile, the secretion of insulin-like growth factors (IGF-1), transforming growth factors (TGF-beta1) and I-type procollagen amino-terminal peptide (PINP) in the body is efficiently promoted, and good market prospects are achieved.

The invention belongs to the field of functional proteomics research, and particularly relates to a combined analysis method for analyzing interaction between a small molecule substance and a targeted protein, which is used for analyzing the small molecule substance and the protein by adopting LiP-MS (Limited Protein-Mass Spectrometry) technology and phosphorylated proteome combined analysis. According to the method, the optimized LiP-MS technology is adopted, the target protein can be directly recognized under the complex proteome background without enriching specific protein, and the problem that low-abundance protein is difficult to detect is solved; phosphorylated proteome abundance transformation protein is analyzed by combining high-resolution mass spectrometry, so that the recognition capability on a drug target and a shear variant thereof is enhanced, and the detection sensitivity is improved.

The invention discloses a single-cell protein extraction and hydrolysis process. The invention discloses a single-cell protein extraction and hydrolysis process which comprises the following steps: S1, adding a chemical reagent into single-cell protein thalli to lyse cells to obtain a cell lysis solution; s2, adding a protein denaturation reagent into the cell lysis solution, and centrifuging to obtain a protein denaturation precipitate; s3, adding a protein dissolving reagent into the protein denaturation precipitate to obtain a protein solution; and S4, adding enzyme into the protein solution, and carrying out protein hydrolysis to obtain a hydrolysate. The method has a wide application prospect, and is expected to promote technical progress and industrial development in related fields.

The present disclosure provides antibodies that bind complement C1s protein; and nucleic acid molecules that encode such antibodies. In some embodiments, such anti-complement C1s antibodies inhibit proteolytic activity of C1s. The present disclosure also provides compositions comprising such antibodies, and methods to produce and use such antibodies, nucleic acid molecules, and compositions.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to promote the growth of soybean plants under flood stress. By selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then apply to the soil containing the soybean at a specific growth stage after germination. Optionally, the KHP solution can be diluted by water as disclosed in the application.

The invention discloses an LC-MS / MS detection method for a palbozuril monoclonal antibody drug in a blood sample, which comprises the following steps: enriching and purifying the palbozuril monoclonal antibody drug in serum and plasma by coupling an enrichment reagent I with magnetic beads, magnetically separating the enriched blood sample, discarding the supernatant, adding a buffer solution I for leaching, and adding a buffer solution II for diluting after leaching; the method comprises the following steps: carrying out high-temperature denaturation on a diluted blood sample, sequentially adding an internal standard and protease for enzymolysis, and then adopting a high performance liquid chromatography-tandem mass spectrometry to realize high-sensitivity rapid detection of the drug concentration of the palbozuril monoclonal antibody in the blood sample. In the pretreatment process, the steps of protein reduction and alkylation are removed, the proteolysis time is shortened, the whole process is simple, convenient and rapid, and multiple requirements of clinical detection on timeliness, accuracy and low detection cost can be met at the same time.

The present invention relates to the technical field of chemical pharmacy, and in particular to a compound for degradation of a targeted interleukin-1 receptor-associated kinase 4 (IRAK4), and a pharmaceutically acceptable salt of the compound. The compound can be used for treating or preventing IRAK4-mediated immune diseases, inflammatory diseases, and cancers.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The present invention relates to a microbial combination and a fermented milk and the use thereof, in particular to a multi-strain microbial combination, a fermented milk prepared from the microbial combination and the use of the microbial combination in the fermentation of a camel milk. The microbial composition contains at least two of lactic acid bacteria and yeasts derived from traditional fermented milk products in Xinjiang, including Leuconostoc pseudomesenteroides, Lactobacillus rhamnosus, Debaryomyces hansenii and Kluyveromyces marxianus. The microbial composition ensures that industrially fermented camel milk products have the flavor and nutritional functions of naturally fermented sour camel milk, but with higher quality, better safety and significantly prolonged shelf life. The microbial composition has relatively high acidification efficiency, proteolysis capacity and lactose decomposition capability, can improve the unpleasant flavor of camel milk products industrially fermented by using conventional strains, and has wide application prospects in industrial production.

The present invention provides engineered leucine decarboxylase (LDC) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered leucine decarboxylase (LDC) polypeptides. In some embodiments, the engineered LDC polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis, and / or increased tolerance to low pH environments. In some embodiments, the engineered LDC polypeptides are optimized to provide improved storage stability. The present invention also provides methods for the use of the compositions comprising the engineered LDC polypeptides for therapeutic and industrial purposes.

Provided herein are novel stabiligases for use in cell membrane proteome analysis. The subject stabiligases are capable of attaching to glycans found on the surface of cell membranes to form glycan-tethered (GT) stabiligases. Such glycan-tethered stabiligases are capable of robustly and selectively attaching label probes to cell surface proteins of intact cells. The subject novel stabiligases described herein advantageously allow for the identification and profiling of cell membrane proteins that have undergone an extracellular N-terminal proteolytic event.

The invention discloses a peptidomics-based computer-aided rapid screening method, collagen antifreeze peptide and application thereof, and relates to the fields of food biotechnology and bioinformatics. The amino acid sequence of the collagen antifreeze peptide is as shown in any one of SEQ ID NO.1-5. The invention provides a computer-aided antifreeze peptide rapid screening method based on peptiomics, which comprises the following steps: performing peptiomics analysis on a cod collagen hydrolysate containing a large amount of polypeptide, and screening the antifreeze peptide according to physicochemical properties, amino acid composition of a primary structure and a molecular docking result of the cod collagen hydrolysate. The interaction capability of the cod collagen hydrolysate and the ice crystals is scored in multiple aspects, so that the antifreeze peptide with strong interaction capability with the ice crystals is rapidly screened. The antifreeze peptide provided by the invention can form a plurality of hydrogen bonds with ice crystals, has extremely strong action capacity with the ice crystals, can be used for preparing an antifreeze agent, and is further applied to frozen surimi products.

The present invention discloses the preparation of an anti-inflammatory peptide derived from the hydrolysis of shell nacre protein and its application in skin repair. The peptide PDFDNGF provided by the invention can effectively promote the proliferation of RAW264.7 macrophage cells and inhibit the excessive production of nitric oxide and cytokines in RAW264.7 cells induced by lipopolysaccharide, while increasing the levels of anti-inflammatory cytokines, demonstrating significant anti-inflammatory activity. Furthermore, the active peptide has a significant proliferative effect on L929 cells, indicating its potential to promote skin wound healing. This invention provides a theoretical basis for the high-value utilization of shell nacre.

Provided are a polypeptide having the function of lowering cholesterol in a soybean protein hydrolysate, and a preparation method therefor and the use thereof. The polypeptide is derived from a peptide fragment having a binding_energy value of < -1.2 Kcal docked to a cholesterol esterase molecule, and the peptide fragment of the polypeptide subjected to molecular docking to cholesterol esterase is derived from 23 polypeptides with the biological activity score of Peptide Ranker > 0.9. Therefore, the polypeptide has a significant blood-lipid-lowering function.

The invention relates to the technical field of agricultural fertilizers, and discloses a preparation method of an amino acid foliar fertilizer extracted based on animal hair, and the preparation method comprises the following steps: raw material pretreatment: carrying out plasma modification on animal hair to improve the keratin hydrolysis performance; a double hydrolysis process: firstly decomposing a keratin structure in the hair through sulfuric acid hydrolysis, and then generating free amino acid through enzyme hydrolysis; recycling by-products: preparing biochar or protein feed from undissolved residues generated in the hydrolysis process, and separating and recycling amino acids and salts in waste liquid; and preparing a foliar fertilizer formula: chelating the extracted amino acid concentrated solution with trace elements. The technical effects of zero emission and high-valued conversion of wastes are achieved through a resource utilization scheme that biochar is prepared from undissolved residues through a pyrolysis process or protein feed is prepared from the undissolved residues through secondary enzymolysis, and free amino acids and salts in waste liquid are separated and recycled through a membrane separation and crystallization extraction technology.

The invention discloses preparation of a shell nacre protein hydrolysis anti-inflammatory peptide and application of the shell nacre protein hydrolysis anti-inflammatory peptide in skin repair. The peptide PDFDNGF provided by the invention can effectively promote proliferation of macrophage RAW264.7 cells, can inhibit excessive production of nitric oxide and cytokines in the RAW264.7 cells induced by lipopolysaccharide, can increase the level of anti-inflammatory cytokines, and shows remarkable anti-inflammatory activity. In addition, the bioactive peptide has a remarkable proliferation effect on L929 cells, and it is indicated that the bioactive peptide has the potential of promoting skin wound healing. The method provides a theoretical basis for high-value utilization of the shell nacre layer.

The invention discloses extraction of polydeoxyribonucleotide as well as preparation and application of a PDRN-Ce (Polydeoxyribonucleotide-Ce) microsphere cluster. The extraction and purification method comprises the following steps: mixing in-vitro fish testis tissues with water, carrying out crushing treatment and homogenization treatment on an obtained premix, carrying out proteolysis and solid-liquid separation, and collecting supernate to obtain enzymatic hydrolysate; wherein protease used for proteolysis comprises neutral protease; carrying out alcohol precipitation on the enzymatic hydrolysate by adopting an alcohol solvent, and collecting a precipitate; and dissolving the precipitate in water, desalting and drying. According to the extraction method, the purity and the yield of the poly-deoxyribonucleotide are remarkably improved, the preparation method is low in cost and convenient to operate, the prepared poly-deoxyribonucleotide is stable in property, small in molecular weight and easy to absorb by skin, in order to further improve the bioavailability of the extracted poly-deoxyribonucleotide, the PDRN-Ce microsphere cluster is prepared, and the PDRN-Ce microsphere cluster can be used for preparing the poly-deoxyribonucleotide. Good application prospects are realized.

The present invention relates to compounds of formula (I), in particular their use as degradation agents for the human papilloma virus E7 protein: # imgabs0 #

The invention relates to the technical field of non-small cell lung cancer treatment, in particular to application of a novel epidermal growth factor receptor degradation protein hydrolysis targeting chimera DC1-206 in preparation of a medicine for treating EGFR mutant non-small cell lung cancer. The structure of the targeted chimera DC1-206 comprises an E3 ubiquitin ligase ligand, a target protein ligand EGFR-TKI and a Linker of a special structure for connecting two active ligands, a triad EGFR-PROTAC active drug molecule is finally formed, the target protein ligand of PROTAC is combined with a target protein in the body of a patient, and the targeted chimera DC1-206 can be used for preparing the targeted chimera DC1-206. According to the present invention, the E3 ubiquitin ligase ligand is combined with the substrate binding region of the E3 ubiquitin ligase in the cell, such that the target protein is pulled to the side of the E3 ubiquitin ligase through the Linker, the target protein is degraded by the UPS system, the lung cancer cell proliferation is effectively inhibited, the lung cancer cell apoptosis is promoted, and the efficient targeting tumor inhibition effect is achieved.

Antigen binding molecules for targeting proteolysis are provided. The molecules generally include (1) a TRIM21 binding profile, (2) a cell penetrating profile of a cell penetrating antibody, and (3) an antigen binding profile that binds to a target protein. The TRIM21 binding feature typically includes a polypeptide sequence sufficient to bind TRIM21 and is effective to direct the target protein to travel along the TRIM21 degradation pathway. The cell penetrating feature of the cell penetrating antibodies is typically to enhance delivery of molecules through the cell membrane. The antigen binding feature binds to the target protein and mediates binding of the molecule to the target protein. In some embodiments, the antigen binding molecules are multispecific antibodies, such as bispecific or trispecific antibodies. Compositions comprising an effective amount of an antigen-binding molecule and methods of using the same are also provided.

The present invention relates to compounds of formula (I) useful for the ex vivo, in vitro or in vivo degradation of BRAF by the ubiquitin proteolysis pathway. The invention also provides pharmaceutically acceptable compositions comprising the compounds, and methods of using the compositions in the treatment of various diseases, disorders, and / or conditions.

The invention relates to methoxy polyethylene glycol epoxidation modified silk fibroin and a preparation method thereof, and belongs to the technical field of biological extraction. The method comprises the following steps: adding a low-concentration hydrogen bond inhibitor and a pH regulator into a regenerated silk fibroin solution, mixing, adding trypsin, incubating, inactivating, carrying out solid-liquid separation, and taking a solid phase to obtain a silk fibroin solid; then mixing and activating with an inorganic salt solution, performing epoxy co-incubation with methoxy polyethylene glycol after activation, and performing purification treatment to obtain a chemically grafted silk fibroin solution. The invention provides a low-concentration hydrogen bond inhibition-pH regulation combined enzymolysis technology, a dissolution modification technology of a precipitation layer after enzymolysis and a technical scheme of inorganic salt solution activation / methoxy polyethylene glycol epoxy synergistic chemical modification for the first time, so that the problems that the proteolysis effect is low and the enzymatic activity cannot be maintained for a long time are solved; and the precipitation layer is successfully prepared into a stable solution, so that the success rate of chemical modification of the silk fibroin is greatly improved, and subsequent further development and use are facilitated.

Proteolysis Targeting Chimeras (PROTACs) are heterobifunctional degraders that specifically eliminate targeted proteins by hijacking the ubiquitin-proteasome system (UPS). Provided are pharmaceutical compositions which include a mixture of a PROTAC, a hydrophilic polymer, a surfactant, and optionally an acid and an adsorbent. Also described are methods for preparing and using such pharmaceutical compositions. In one aspect, disclosed herein is an amorphous solid dispersion comprising PROTAC.

The invention relates to a cysteine protease structural domain mediated protein C-terminal selective modification method. The method comprises the following steps: adding a nucleophilic reagent, a catalyst and a cysteine protease allosteric activator into a target protein fused with a cysteine protease structural domain (CPD) as shown in a formula (I), and releasing a C-terminal modified target protein while inducing CPD splitting decomposition. The invention also provides a CPD-catalyzed proteolysis method and a C-terminal modification conversion method on the basis of the C-terminal modification. The method disclosed by the invention has high flexibility and has a huge application prospect in C-terminal modification of protein molecules. # imgabs0 #

The invention relates to the technical field of biochemistry, in particular to a specific affinity enrichment and preparation method of a torreya grandis protease hydrolysate ACE inhibitory peptide, which comprises the following steps: step 1, preparing and characterizing magnetic aminated Fe3O4 nanoparticles; step 2, preparing and characterizing the immobilized trypsin; 3, preparing and characterizing the immobilized ACE, and optimizing reaction conditions; step 4, determining the activity of the immobilized ACE; step 5, then preparing and specifically enriching the Chinese torreya antihypertensive peptide; step 6, then, carrying out molecular docking through AutoDock Vina; according to the method, genipin is adopted as a cross-linking agent, magnetic Fe3O4 nanoparticles with the ACE inhibitory peptide specific affinity recognition function are constructed, specific enrichment of the ACE inhibitory peptide from the torreya grandis cake protein hydrolysate is achieved, and specific separation is achieved.

The invention discloses a proteolysis targeted coupling nano antibody PROTNC as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention aims to provide a micromolecule coupled nano antibody type degradation agent which can degrade intracellular protein in a targeted manner. The invention provides a proteolysis targeted coupled nano antibody PROTNC. A degradation agent is prepared from the following components: a nano antibody, a connexon, an E3 ubiquitin ligase small molecule ligand and an intracellular delivery module. The PROTNC can be used for degrading intracellular proteins in a targeted manner, and particularly can be used for realizing targeted degradation on inherent disordered proteins.

The invention relates to the field of biological medicine, in particular to a polypeptide composition with bacteriostatic activity and a preparation method thereof.The polypeptide composition is prepared by wrapping polypeptide I with modified polyethylene glycol, then wrapping with nano-silver and finally modifying polypeptide II with the nano-silver. The outer layer is sequentially wrapped with spherical particles of modified polyethylene glycol, nano-silver and polypeptide II, and the polypeptide II and the nano-silver are promoted to be combined with a bacterial membrane and destroy the bacterial membrane through the non-specific electrostatic interaction of the polypeptide II and the nano-silver, so that the polypeptide II has relatively high antibacterial performance; the outer-layer polypeptide is degraded by proteolytic enzyme or plays a role with a bacterial membrane, so that the inner-layer polypeptide I wrapped by the modified polyethylene glycol is exposed, the antibacterial effect is further played, and the proteolytic stability of the polypeptide is improved; due to the spherical structure, the prepared polypeptide composition with the antibacterial activity has excellent antibacterial performance, meanwhile, the stability can be improved, and the polypeptide composition has wide application prospects.

The present disclosure provides DNase enzymes with reduces susceptibility to proteolysis and / or which provide improved in vivo stability or half-life. In accordance with aspects of the disclosure, the DNase enzymes described herein are more physiologically stable and thus are suitable for therapy with reduced doses and / or less frequent dosing. In embodiments, the DNase enzymes have benefits for systemic therapy, which include longer exposure and extended duration of pharmacodynamic action.

The invention belongs to the technical field of bioengineering and application thereof, and particularly relates to a laver compound zymolyte as well as a preparation method and application thereof. The compound enzyme is prepared through a compound enzymolysis process of protease and laver polysaccharide enzyme. Compared with a traditional single proteolysis process, a composite enzymolysis process of adding the high-enzyme-activity laver polysaccharide enzyme effectively breaks a'polysaccharide-protein 'composite structure of laver cell walls, so that the viscosity of zymolyte is reduced, high laver liquefaction rate and high product yield are realized, and the release efficiency of nutritional active ingredients is remarkably improved; the product is rich in low-molecular-weight polypeptide and oligomeric porphyra polysaccharide. The obtained zymolyte shows the comprehensive effects of improving joint activity, inhibiting osteophyte formation, adjusting cartilage metabolism balance and relieving inflammatory response in an osteoarthritis mouse model, and osteoarthritis is effectively relieved. According to the invention, efficient release and utilization of nutritional active ingredients of the laver are realized, and powerful technical support is provided for developing high-valued products, such as health-care products and functional foods, which have the function of maintaining joint health.