363 results about "Proteolysis" patented technology

The present invention relates to various molecular constructs having at least one cellular-targeting element, one protein-targeting element, and one ubiquitin ligase recruitment element, wherein these elements are conjugated in a manner that facilitates cellular uptake and targeted degradation of specific proteins within cells. The present invention further relates to multifunctional proteolysis-targeting chimera conjugates and uses thereof for the treatment of diseases or disorders such as immune and inflammatory diseases, infectious diseases, cardiovascular diseases, endocrine disorders, and various cancers that are otherwise resistant to traditional therapeutic regimens.

The invention belongs to the field of functional proteomics research, and particularly relates to a combined analysis method for analyzing interaction between a small molecule substance and a targeted protein, which is used for analyzing the small molecule substance and the protein by adopting LiP-MS (Limited Protein-Mass Spectrometry) technology and phosphorylated proteome combined analysis. According to the method, the optimized LiP-MS technology is adopted, the target protein can be directly recognized under the complex proteome background without enriching specific protein, and the problem that low-abundance protein is difficult to detect is solved; phosphorylated proteome abundance transformation protein is analyzed by combining high-resolution mass spectrometry, so that the recognition capability on a drug target and a shear variant thereof is enhanced, and the detection sensitivity is improved.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to promote the growth of soybean plants under flood stress. By selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then apply to the soil containing the soybean at a specific growth stage after germination. Optionally, the KHP solution can be diluted by water as disclosed in the application.

The present invention relates to the technical field of chemical pharmacy, and in particular to a compound for degradation of a targeted interleukin-1 receptor-associated kinase 4 (IRAK4), and a pharmaceutically acceptable salt of the compound. The compound can be used for treating or preventing IRAK4-mediated immune diseases, inflammatory diseases, and cancers.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The present invention relates to a microbial combination and a fermented milk and the use thereof, in particular to a multi-strain microbial combination, a fermented milk prepared from the microbial combination and the use of the microbial combination in the fermentation of a camel milk. The microbial composition contains at least two of lactic acid bacteria and yeasts derived from traditional fermented milk products in Xinjiang, including Leuconostoc pseudomesenteroides, Lactobacillus rhamnosus, Debaryomyces hansenii and Kluyveromyces marxianus. The microbial composition ensures that industrially fermented camel milk products have the flavor and nutritional functions of naturally fermented sour camel milk, but with higher quality, better safety and significantly prolonged shelf life. The microbial composition has relatively high acidification efficiency, proteolysis capacity and lactose decomposition capability, can improve the unpleasant flavor of camel milk products industrially fermented by using conventional strains, and has wide application prospects in industrial production.

The present invention discloses the preparation of an anti-inflammatory peptide derived from the hydrolysis of shell nacre protein and its application in skin repair. The peptide PDFDNGF provided by the invention can effectively promote the proliferation of RAW264.7 macrophage cells and inhibit the excessive production of nitric oxide and cytokines in RAW264.7 cells induced by lipopolysaccharide, while increasing the levels of anti-inflammatory cytokines, demonstrating significant anti-inflammatory activity. Furthermore, the active peptide has a significant proliferative effect on L929 cells, indicating its potential to promote skin wound healing. This invention provides a theoretical basis for the high-value utilization of shell nacre.

The invention discloses extraction of polydeoxyribonucleotide as well as preparation and application of a PDRN-Ce (Polydeoxyribonucleotide-Ce) microsphere cluster. The extraction and purification method comprises the following steps: mixing in-vitro fish testis tissues with water, carrying out crushing treatment and homogenization treatment on an obtained premix, carrying out proteolysis and solid-liquid separation, and collecting supernate to obtain enzymatic hydrolysate; wherein protease used for proteolysis comprises neutral protease; carrying out alcohol precipitation on the enzymatic hydrolysate by adopting an alcohol solvent, and collecting a precipitate; and dissolving the precipitate in water, desalting and drying. According to the extraction method, the purity and the yield of the poly-deoxyribonucleotide are remarkably improved, the preparation method is low in cost and convenient to operate, the prepared poly-deoxyribonucleotide is stable in property, small in molecular weight and easy to absorb by skin, in order to further improve the bioavailability of the extracted poly-deoxyribonucleotide, the PDRN-Ce microsphere cluster is prepared, and the PDRN-Ce microsphere cluster can be used for preparing the poly-deoxyribonucleotide. Good application prospects are realized.

The present invention relates to compounds of formula (I), in particular their use as degradation agents for the human papilloma virus E7 protein: # imgabs0 #

The invention relates to the technical field of non-small cell lung cancer treatment, in particular to application of a novel epidermal growth factor receptor degradation protein hydrolysis targeting chimera DC1-206 in preparation of a medicine for treating EGFR mutant non-small cell lung cancer. The structure of the targeted chimera DC1-206 comprises an E3 ubiquitin ligase ligand, a target protein ligand EGFR-TKI and a Linker of a special structure for connecting two active ligands, a triad EGFR-PROTAC active drug molecule is finally formed, the target protein ligand of PROTAC is combined with a target protein in the body of a patient, and the targeted chimera DC1-206 can be used for preparing the targeted chimera DC1-206. According to the present invention, the E3 ubiquitin ligase ligand is combined with the substrate binding region of the E3 ubiquitin ligase in the cell, such that the target protein is pulled to the side of the E3 ubiquitin ligase through the Linker, the target protein is degraded by the UPS system, the lung cancer cell proliferation is effectively inhibited, the lung cancer cell apoptosis is promoted, and the efficient targeting tumor inhibition effect is achieved.

The present invention relates to compounds of formula (I) useful for the ex vivo, in vitro or in vivo degradation of BRAF by the ubiquitin proteolysis pathway. The invention also provides pharmaceutically acceptable compositions comprising the compounds, and methods of using the compositions in the treatment of various diseases, disorders, and / or conditions.

The invention relates to methoxy polyethylene glycol epoxidation modified silk fibroin and a preparation method thereof, and belongs to the technical field of biological extraction. The method comprises the following steps: adding a low-concentration hydrogen bond inhibitor and a pH regulator into a regenerated silk fibroin solution, mixing, adding trypsin, incubating, inactivating, carrying out solid-liquid separation, and taking a solid phase to obtain a silk fibroin solid; then mixing and activating with an inorganic salt solution, performing epoxy co-incubation with methoxy polyethylene glycol after activation, and performing purification treatment to obtain a chemically grafted silk fibroin solution. The invention provides a low-concentration hydrogen bond inhibition-pH regulation combined enzymolysis technology, a dissolution modification technology of a precipitation layer after enzymolysis and a technical scheme of inorganic salt solution activation / methoxy polyethylene glycol epoxy synergistic chemical modification for the first time, so that the problems that the proteolysis effect is low and the enzymatic activity cannot be maintained for a long time are solved; and the precipitation layer is successfully prepared into a stable solution, so that the success rate of chemical modification of the silk fibroin is greatly improved, and subsequent further development and use are facilitated.

Proteolysis Targeting Chimeras (PROTACs) are heterobifunctional degraders that specifically eliminate targeted proteins by hijacking the ubiquitin-proteasome system (UPS). Provided are pharmaceutical compositions which include a mixture of a PROTAC, a hydrophilic polymer, a surfactant, and optionally an acid and an adsorbent. Also described are methods for preparing and using such pharmaceutical compositions. In one aspect, disclosed herein is an amorphous solid dispersion comprising PROTAC.

The invention relates to the technical field of biochemistry, in particular to a specific affinity enrichment and preparation method of a torreya grandis protease hydrolysate ACE inhibitory peptide, which comprises the following steps: step 1, preparing and characterizing magnetic aminated Fe3O4 nanoparticles; step 2, preparing and characterizing the immobilized trypsin; 3, preparing and characterizing the immobilized ACE, and optimizing reaction conditions; step 4, determining the activity of the immobilized ACE; step 5, then preparing and specifically enriching the Chinese torreya antihypertensive peptide; step 6, then, carrying out molecular docking through AutoDock Vina; according to the method, genipin is adopted as a cross-linking agent, magnetic Fe3O4 nanoparticles with the ACE inhibitory peptide specific affinity recognition function are constructed, specific enrichment of the ACE inhibitory peptide from the torreya grandis cake protein hydrolysate is achieved, and specific separation is achieved.

The invention belongs to the technical field of bioengineering and application thereof, and particularly relates to a laver compound zymolyte as well as a preparation method and application thereof. The compound enzyme is prepared through a compound enzymolysis process of protease and laver polysaccharide enzyme. Compared with a traditional single proteolysis process, a composite enzymolysis process of adding the high-enzyme-activity laver polysaccharide enzyme effectively breaks a'polysaccharide-protein 'composite structure of laver cell walls, so that the viscosity of zymolyte is reduced, high laver liquefaction rate and high product yield are realized, and the release efficiency of nutritional active ingredients is remarkably improved; the product is rich in low-molecular-weight polypeptide and oligomeric porphyra polysaccharide. The obtained zymolyte shows the comprehensive effects of improving joint activity, inhibiting osteophyte formation, adjusting cartilage metabolism balance and relieving inflammatory response in an osteoarthritis mouse model, and osteoarthritis is effectively relieved. According to the invention, efficient release and utilization of nutritional active ingredients of the laver are realized, and powerful technical support is provided for developing high-valued products, such as health-care products and functional foods, which have the function of maintaining joint health.

This document provides methods and materials involved in treating cancer. For example, methods and materials for using (a) one or more antibody-drug conjugates (ADCs) and (b) one or more proteolysis-targeting chimera (PROTAC) compounds to treat a mammal (e.g., a human) having cancer are provided.

The present disclosure relates to bifunctional compounds, which find utility as modulators of Protein Arginine N-Methyl Transferase 5 (PRMT5) in MTAP deleted / low expressing cells. In particular, the present disclosure is directed to bifunctional compounds, which contain on one end a ligand which binds to an E3 ubiquitin ligase and on the other end a moiety which binds the PRMT5-MTA complex, such that the PRMT5 protein is placed in proximity to the ubiquitin ligase to effectuate ubiquitination, and therefore, degradation (and inhibition) of PRMT5 protein, as well as methods for their preparation and use. Specifically, the present invention describes compounds of Formula (I) and pharmaceutically acceptable salts, hydrates and solvates, as well as methods for their preparation and use

The present disclosure relates to enzymes, compositions and methods for performing conditional homologous recombination of a targeted DNA molecule or genome by using modified proteins comprising nucleic acid DNA binding proteins with protease-conditional recombinase (hereinafter “ProRec”) activity. Namely a new cre recombinase pro enzyme, compositions / kit and sensors comprising the cre recombinase pro enzyme of the present disclosure. Namely, a cre recombinase pro-enzyme, for identifying and quantifying proteolytic activity in a disease or an infection, comprising intein mediated circularization, a flip-excision cassette comprising an output reporter and / or effector protein; a linker sequence cleavable by a protease.

The invention provides a pill-type organic fertilizer special for vegetables. The pill-type organic fertilizer is prepared from the following raw materials in parts by weight: modified biochar, fermented livestock and poultry manure, plant source protein zymolyte, a complex microbial inoculant, a trace element supplement and a binder, and a method therefor; the complex microbial inoculants comprise actinomycetes, bacillus amyloliquefaciens and trichoderma harzianum; the trace element supplement comprises EDTA (Ethylene Diamine Tetraacetic Acid) chelated zinc, EDTA chelated iron and boric acid. Therefore, on one hand, the organic fertilizer can effectively improve the facility agriculture soil environment, adjust the soil acidity and alkalinity, reduce the salinization degree, promote balance and stability of microbial communities and restore and improve the ecological function of soil; on the other hand, the utilization rate of the fertilizer can be greatly improved, and the plant-source proteolysis provides a quick-acting nitrogen source to promote root development; the microelement supplement meets the requirements of vegetables on microelements; nutrients are balanced, vegetable stress resistance is enhanced, and photosynthetic efficiency is improved; and the binder enables the fertilizer to form uniform particles, is suitable for mechanical fertilization, and improves the fertilization efficiency.

The present disclosure provides antibodies that bind complement C1s protein; and nucleic acid molecules that encode such antibodies. In some embodiments, such anti-complement C1s antibodies inhibit proteolytic activity of C1s. The present disclosure also provides compositions comprising such antibodies, and methods to produce and use such antibodies, nucleic acid molecules, and compositions.

The invention relates to the field of food materials, and particularly discloses a gamma-aminobutyric acid and hydrolyzed egg yolk powder composite beverage capable of promoting sleep and inhibiting bone age and a preparation method of the gamma-aminobutyric acid and hydrolyzed egg yolk powder composite beverage. A gamma-aminobutyric acid and hydrolyzed egg yolk powder composite beverage capable of promoting sleep and inhibiting bone age comprises the following raw materials in percentage: 3-5% of xylitol, 3-5% of fructo-oligosaccharide, 2-4% of gamma-aminobutyric acid, 3-9% of antioxidant composite concentrated juice, 0.16-1% of purple carrot juice beverage thick slurry, 0.1-0.19% of calcium aspartate, 0.02-0.05% of casein hydrolyzed peptide, 0.02-0.05% of hydrolyzed egg yolk powder, 0.02-0.05% of casein phosphopeptide and the balance of water. The formula comprises the following components in percentage by weight: 0.006 to 0.01 percent of zinc gluconate, 0.0005 to 0.00085 percent of zinc-rich edible yeast powder, 0.0005 to 0.00085 percent of tea theanine, 0.0005 to 0.00085 percent of N-acetylneuraminic acid, 0.0018 to 0.09 percent of a traditional Chinese medicine composition, 0.005 to 0.01 percent of L-calcium lactate, 0.0005 to 0.01 percent of collagen peptide, 0.1 to 0.3 percent of xanthan gum, 0.0005 to 0.00085 percent of melissa officinalis, 0.2 to 0.98 percent of a compound flavoring agent and the balance of purified water. Through the synergistic effect of all the components, the sleep quality is effectively improved, overactivity of bone cells is inhibited, and the bone age development is delayed.

The invention discloses a CDK2 / 4 / 6 proteolysis targeting chimera as well as a preparation method, a pharmaceutical composition and application thereof, and the chemical structure of the CDK2 / 4 / 6 proteolysis targeting chimera is shown as a formula I, the CDK2 / 4 / 6 proteolysis targeting chimera or the isomer, the pharmaceutically acceptable salt, the solvate, the prodrug, the deuterated compound or the polymorphic substance thereof can induce selective degradation of CDK2, CDK4 and CDK6 through ubiquitin-proteasome, so that the phosphorylation level of RB1 is reduced, release of a transcription factor E2F is further inhibited, the process of cells from a G1 phase to an S phase is blocked, and the targeting effect of the cells is improved. Compared with an inhibitor, the compound is more efficient and low in toxicity, and can be used for treating various cancers. # imgabs0 #

Provided herein are compounds of formula (I), or their tautomers, stereoisomers, or pharmaceutically acceptable salts. These compounds are useful as KRAS(G12D) proteolytic agents and / or inhibitors. [Case 1] TIFF2026511222000314.tif64165

This text describes bifunctional compounds that can be used as modulators of non-receptor leucine-rich repeat kinase 2 (LRRK2). In particular, the bifunctional compounds of the present disclosure contain a moiety that binds to the cereblon E3 ubiquitin ligase at one end and a moiety that binds to LRRK2 at the other end, such that the target protein is placed in proximity to the ubiquitin ligase to effect degradation (and inhibition) of the target protein. The bifunctional compounds of the present disclosure exhibit a broad range of pharmacological activities related to degradation / inhibition of the target protein. Treat or prevent diseases or disorders caused by abnormal regulation of the target protein with the compounds and compositions of the present disclosure.

The invention discloses a preparation method of a red rice bran protein peptide compound with antioxidant and hypoglycemic effects. According to the method, red rice bran protein is extracted through an alkali-solution and acid-isolation method; hydrolyzing the red rice bran protein with alkaline protease to obtain a red rice bran protein hydrolysate; the red rice bran protein hydrolysate prepared by the invention has the functional characteristics of excellent emulsibility, foamability and oil absorbency, and has excellent antioxidant and hypoglycemic functional activities after being subjected to in-vitro simulated gastrointestinal digestion. The red rice bran protein hydrolysate can be used in plant-based food and dairy product substitutes and functional food with a hypoglycemic effect.

The invention discloses a method for synergistically improving the bleeding problem in the storage process of pea starch-quinoa protein gel through proteolysis and gellan gum addition. The invention provides a method for preparing gel. The method comprises the following steps: (1) mixing enzymolysis quinoa protein, pea starch, gellan gum and water to obtain a mixed suspension; and (2) heating and gelatinizing the mixed suspension, and cooling and molding to obtain the gel. The gel prepared by the method has the beneficial effects that the water binding capacity is high, and the bleeding rate is low; microscopic structure observation shows that a highly compact and uniform network is formed, and no obvious bubbles exist; low-field nuclear magnetic resonance analysis shows that the bound water proportion is obviously increased; a dynamic rheological test proves that the gel has excellent gel stability; and the process is simple and safe and easy for industrial production. The invention provides a reliable technical scheme for the development of high-protein nutritional soft food for old people.

The microorganism strain Pediococcus acidilactici TAK 589 CoccobEst is a new antimicrobial and antioxidant probiotic, CoccobEst inhibits the growth of the enteropathogens L. monocytogenes, E. coli, S. enteritidis, S. typhimurium, S. sonnei, S. aureus, E. faecalis and C. sakazakii, but does not inhibit lactobacilli of natural intestinal microbiota. The strain's antimicrobial properties are enhanced by periplasmic peptidoglycan hydrolases with proteolytic activity. CoccobEst is used as a zootechnieai feed additive to prevent bacterial infections in the gastrointestinal tract of farm animals and pets and to prevent and reduce diarrhea. CoccobEst has highly active antioxidant defense system, increasing the redox activity of glutathione and reducing oxidative stress in cells. Therefore, CoccobEst is used as a food supplement or functional food for humans to prevent bacterial infections of the gastrointestinal tract and to prevent and reduce oxidative stress. In addition, CoccobEst is used as a starter culture in fermentation of vegetables.

The invention discloses a method for preparing a soybean protein hydrolysate-soybean seed coat polysaccharide compound based on ultrasonic treatment.The method belongs to the technical field of food processing and manufacturing, soybean protein isolate hydrolysate with a balanced structure is obtained by controlling enzymolysis conditions, then the soybean protein isolate hydrolysate is compounded with soybean seed coat polysaccharide, moderate ultrasonic treatment is combined, and the soybean protein hydrolysate-soybean seed coat polysaccharide compound is obtained. Compared with the prior art, the preparation method has the advantages that the emulsifying property is improved while the protein structure functionality is maintained, and the preparation method is simple in process, green, environmentally friendly and capable of achieving large-scale production and is suitable for functional emulsion food, active substance delivery and development of other emulsion-based products.

Use of glycogen synthase kinase-3 (GSK3) as a protease, and a GSK3-based proteolysis-targeting chimera (PROTAC) and a preparation method and use thereof are provided, belonging to the technical field of proteolysis. The GSK3 and an N-terminal domain, an intermediate domain, or a C-terminal domain thereof have a protease activity and can achieve efficient proteolysis. Based on the GSK3 and other similar proteases, PROTACs can be developed to achieve efficient targeted proteolysis of a target protein.

The present disclosure can improve the degree of soy protein proteolysis and the ability to produce valine, isoleucine, and leucine, which are branched-chain amino acids, as well as other amino acids, such as threonine, glycine, tyrosine, and lysine. Accordingly, the present disclosure can prevent sarcopenia and has antioxidant activity. Also, the present disclosure can maintain protein metabolic balance and relieve sarcopenia by muscle synthesis.The self-aggregation, hydrophobicity, and intestinal adhesion of a mixed strain is improved compared to a case where a single strain used for the mixed strain is used. Therefore, when the mixed strain of the present disclosure is ingested, the strain can remain for a long time in the intestine.