7 results about "Aminoacylation" patented technology

The invention relates to aminoacyl-tRNA synthetases that aminoacylate tRNA with 2-Aminoisobutyric acid (Aib), thus enabling the incorporation of the Aib into a growing polypeptide chain during translation, e.g. in eubacterial host cells such as E. coli. For example, but not limited to, the invention relates to new aminoacyl-tRNA synthetases and the uses hereof, as well as a method for producing polypeptides that contain one or more Aib.

The present inventive concept relates to methods of nucleic acid sequencing, and applications and uses of the same. Aspects of the inventive concept include nanopore sequencing on nucleic acids, such as ribonucleic acids (RNAs), including, but not limited to, transfer RNAs (tRNAs). Further aspects include kits for performing the methods, and applications of the methods of the inventive concept as substantively described herein.

The present invention provides novel active esters of amino acids for use in aminoacylation of tRNAs with reduced safety and waste risks.SOLUTION: The present invention provides a compound represented by formula (I): or a salt thereof, in particular dichloropyridinylmethyl ester and 2,2,2 - trifluoroethyl ester or a salt thereof.SELECTED DRAWING: None

The application discloses a protein lysine valylated pan-antibody and a preparation method and application thereof, relates to the technical field of biology, and discloses a polyclonal antibody, wherein an epitope is a valyl group on an epsilon-amino group of a lysine residue in a protein or polypeptide; the pan-antibody has sequence-independent recognition of the valyl group, and does not cross-react with unmodified lysine residues and methionylated lysine residues; the pan-antibody has sequence-independent recognition characteristics, so that researchers can realize qualitative and quantitative analysis of the modification by using conventional immunological means, and the pan-antibody provides a key material basis for revealing the biological functions of valylated modification in tumorigenesis, metabolic regulation and cardiovascular diseases.

The present invention provides a novel amino acid active ester for use in the aminoacylation of a tRNA, said ester being capable of reducing safety and waste risks. The present invention provides a compound represented by formula (I):or a salt thereof, in particular dichloropyridinyl methyl ester and 2,2,2-trifluoroethyl ester or a salt thereof.

This invention relates to the field of biotechnology, specifically to a method for constructing high-throughput sequencing libraries, a kit, and a method for detecting aminoacylated tsRNA. The method for constructing high-throughput sequencing libraries includes the following steps: (1) first-level enzymatic treatment; (2) second-level chemical treatment; (3) third-level enzymatic repair; (4) library construction. This invention constructs a triple cascade treatment system of "specific enzyme digestion labeling - denaturation deaminoacylation - enzymatic modification and repair", which overcomes the three major technical bottlenecks that have long existed in the sequencing of transport tRNA-derived fragments: "signal missed detection", "full-length truncation" and "confusion of active state".

The invention belongs to the field of functional feed additives, and provides a preparation method of liquid fermentation soybean peptide and application of the liquid fermentation soybean peptide in feed. A six-step process of pre-dissolving, directional enzymolysis, gamma-glutamyl, cascade tangential flow filtration and steady-state treatment is adopted. Narrow molecular weight distribution is realized through compounding of neutral endo-protease and exo-aminopeptidase; gamma-glutamyltranspeptidase is introduced to enhance the flavor activity; a target molecular weight segment is accurately enriched through a two-stage membrane; mild enzyme deactivation and sterilization are performed to retain active peptides; the pH is adjusted to 3.5-5.5, so that residual enzyme activity and microbial growth are inhibited, and the shelf life stability of the product is improved. According to the process, highly controllable distribution and functional modification of 60-90% of peptide with the molecular weight of 500-3000Da, 30-70% of gamma-glutamyl peptide and 70-100% of peptide with the molecular weight of less than 1000Da are realized, and the contradiction of low-viscosity processing, mild enzyme deactivation, shelf stability, tangential flow grading flux and pollution resistance under the condition of relatively high solid content is solved; the palatability and the flavor stability of the feed are remarkably improved, and the application value in pig feed is wide.