34results about How to "Valid mark" patented technology

The invention discloses fluorescence amino carbon quantum dots, and a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing aqua ammonia with purified xylan, and stirring until the xylan is dispersed in the solution; (2) transferring the mixture into a reaction kettle, putting in a sealed muffle furnace, and carrying out hydrothermal treatment; (3) cooling the product to room temperature, and carrying out ultrasonic dispersion treatment; (4) carrying out refrigerated centrifugal separation on the treated product, removing the precipitate, and collecting the supernate to obtain a fluorescence amino carbon quantum dot water solution; and (5) concentrating the product water solution by rotary evaporation, and finally, carrying out vacuum drying to obtain the fluorescence amino carbon quantum dots. The amino carbon quantum dots have the advantages of high fluorescence, high stability, low cytotoxicity and favorable biocompatibility, and can be widely used in the fields of cell imaging, disease diagnosis, drug transfer and the like.

The invention relates to an aminoacyl-tRNA (transfer ribonucleic acid) synthetase mutant containing an amino acid sequence selected from groups consisting of amino acids shown by SEQ ID NO:4 and conservative variants thereof. The invention provides a CpK (short for N<epsilon>-(1-methylcyclopropyl-2-acrylamide)-lysine) translation system for fixed point specific insertion of N<epsilon>-(1-methylcyclopropyl-2-acrylamide)-lysine in a target protein through pairing of orthogonal tRNA and orthogonal aminoacyl-tRNA synthetase, and a method for fixed point specific insertion of CpK in the target protein by using the translation system. The CpK translation system comprises (i) N<epsilon>-(1-methylcyclopropyl-2-acrylamide)-lysine, (ii) the orthogonal aminoacyl-tRNA synthetase, (iii) the orthogonal tRNA and (iv) a nucleic acid for coding the target protein, wherein the orthogonal aminoacyl-tRNA synthetase is used for realizing aminoacylation of the orthogonal tRNA preferentially by using CpK, and the nucleic acid contains at least one selective codon specifically identified by the orthogonal tRNA. The invention also relates to an application of a mutant protein for fixed point specific insertion of CpK in protein labeling through a light click reaction with a tetrazole compound.

The invention discloses a method for performing correction on a belief propagation algorithm, comprising steps of obtaining an initial parameter when a iterations is k, updating bit information outputted by a bit node n and verification information outputted by a verification node m according to an initial parameter, enabling k=k+1, updating hard decision information of various bit nodes in order to obtain the value of the nth element of the hard decision vector at the kth interation and outputting a coding sequence according to a calculated value or executing flipping decoding. The invention can effectively mark the key trap set element scale causing the decoding leveling, effectively reduces the fault leveling of the simulation code work, has lower calculation complexity and good coding possibility and can better satisfy the high reliability transmission requirement of other communication systems like the optical transmission.

The invention provides an acrylyl lysine translation system which dopes acrylyl lysine into target protein by using an orthogonal tRNA, an orthogonal acrylyl lysine aminoacyl tRNA synthetase and their combination, and also provides a method for specifically doping acrylyl lysine into target protein at a fixed point by using the translation system. The acrylyl lysine translation system comprises: (i) acrylyl lysine; (ii) the orthogonal acrylyl lysine aminoacyl tRNA synthetase; (iii) the orthogonal tRNA, wherein the orthogonal acrylyl lysine aminoacyl tRNA synthetase is used for preferential aminoacylation of the orthogonal tRNA by the acrylyl lysine; and (iv) nucleic acid coding the target protein, wherein the nucleic acid contains at least one selection codon specifically recognized by the orthogonal tRNA. The invention also relates to an application of the mutant protein doped with the acrylyl lysine.

The invention discloses a digital restoration method for flaking diseases of ancient wall paintings based on compressed sensing, and an intelligent terminal system. The method employs a wall painting image preprocessing module, a wall painting flaking disease marking module and a wall painting image restoration module. The method comprises the steps: enabling the wall painting image preprocessing module to carry out the image denoising and HSV color space transformation, enabling the wall painting flaking disease marking module to achieve the V component extraction of a wall painting image, drawing a brightness contour map through a contour function, carrying out the threshold segmentation of the brightness contour map, and obtaining wall painting segmented images; obtaining a closed interval of a disease region through the morphological corrosion operation, carrying out the overlapping of the segmented images and a damaged wall painting image, and marking the flaking disease region of the wall painting; enabling the wall painting image restoration module to carry out the restoration of the flaking disease region of the wall painting through an image restoration algorithm based on compressed sensing of a PMLE mechanism and an EM mechanism. The method can independently complete the marking of the flaking disease region in the ancient wall painting and the intelligent restoration, and provides support for the digital virtual display of the wall painting.

The invention belongs to the field of fluorescent imaging reagents and relates to a dark red fluorescent active ester IR640B-NHS which can be rapidly marked, and a precursor IR640B of the ester. According to the ester, 2,3,3-trimethylindole is adopted as a matrix which reacts with raw materials such as butane sultone, then a cyanine type fluorescent group with a sulfonic group is synthesized, further through a Suzuki-Miyaura reaction, phenyl carboxylic acid is introduced into a fluorescent group through a carbon-carbon bond, and the phenyl carboxylic acid is modified to generate an N-hydroxylcarboxyfluorescein diacetate succinimidyl ester. The active ester can react with a primary amino group in biological macromolecules under physiological conditions, and then dark red fluorescent groupscan be marked on target molecules through amide bonds. By adopting the ester, biological macromolecules such as polypeptide, proteins, antibodies or polymer molecules can be rapidly, safely, effectively and stably marked, in-vitro evaluation on acceptor targeting properties and intracellular distribution of target biological macromolecules can be implemented by using a fluorescence microscope, aflow cytometer and the like, and nondestructive monitoring and quantitative tracing on target molecules can be achieved through living optical imaging.

The invention discloses a high-fluorescence quantum efficiency red-light polymer, quantum dot solution and an application. A structural formula of the high-fluorescence quantum efficiency red-light polymer is as shown in the specification, and the weight-average molecular weight of the polymer is 35kg/mol. The high-fluorescence quantum efficiency red-light polymer transmits fluorescence in a deepand near infrared area (650nm-900nm), a high-fluorescence quantum efficiency red-light polymer quantum dot with particle diameter smaller than 20nm is prepared from the polymer by a nano re-precipitation method, and a cell micro-tubular protein structure is successfully and effectively marked. A cytotoxicity test proves that the high-fluorescence quantum efficiency red-light polymer quantum dot has low influence on cell activity and good biocompatibility and provided with a near infrared fluorescence probe for in-vivo imaging.

The invention provides a method for separating human intervertebral disc PROCR < + > nucleus pulposus progenitor cells, which comprises the following steps of: 1) fully cutting nucleus pulposus into pieces, and sequentially and respectively digesting the nucleus pulposus pieces with trypsin, streptavidin and type II collagenase to obtain a cell suspension; 2) carrying out erythrocyte splitting decomposition treatment on the cell suspension, carrying out magnetic bead sorting to remove tissue impurities, and resuspending cells with PBS + 0.04% BSA to obtain a cell resuspension; and 3) dyeing the cells in the cell resuspension with PROCR flow cytometry antibodies, sorting through a flow cytometry, comparing the sorting efficiency of two mainstream PROCR clone number antibodies, optimizing the sorting scheme, and finally obtaining the human intervertebral disc PROCR + nucleus pulposus progenitor cells. The invention also provides the human intervertebral disc PROCR < + > nucleus pulposus progenitor cell obtained according to the separation method and application thereof. The nucleus pulposus PROCR < + > cells prepared by the invention have good clone formation, self-renewal and three-line differentiation capacities, and a brand new seed cell source is provided for a biological treatment strategy of intervertebral disc degenerative diseases.

The invention discloses application of polyphyllin I in lysosome detection. Experiments prove that the polyphyllin I has a specific lysosome targeting effect; and in order to facilitate observation and detection, the polyphyllin I and a fluorescent dye are further synthesized into a red fluorescent probe which is named as Polyphyllin I-CY3, and the structural formula of the polyphyllin I-CY3 is shown in (II) that is described in the specification. The polyphyllin I-CY3 red fluorescent probe synthesized by the invention can permeate cell membranes, can be used for specific fluorescent staining of living cell lysosome, and has the advantages of good targeting property, high detection speed, good repeatability, convenience in use, no cytotoxicity and the like.

The invention relates to an automatic hub surface defect detection device which comprises a to-be-detected hub conveying unit, a hub outer side defect detection unit, a hub inner side defect detection unit and a detected hub conveying unit. Hubs are conveyed to the hub outer side defect detection unit through the to-be-detected hub conveying unit, and hub outer side defect detection and marking tasks are completed. The hub is further conveyed to a hub inner side defect detection unit to complete hub inner side defect detection and marking tasks; and finally, the hub is conveyed to a detected hub conveying unit, and a qualified product and defective product sorting task is completed. According to the hub surface defect automatic detection device, region division is carried out on the hub based on the characteristics of a hub rotating body, the hub surface defect automatic detection device is provided according to the division result, full automation of hub inner and outer surface defect detection and marking is achieved through a region division detection mode, the hub defect detection and marking efficiency is effectively improved, and the hub surface defect automatic detection device has good practical application value.

The invention discloses a tumor cell proliferation detection method based on Hochest333258 and application. The method comprises the following steps: subculturing tumor cells to a cell culture plate,counting cells, then carrying out doubling dilution, so that a gradient series with different cell numbers is obtained, carrying out the conventional culture in a culture medium, then adding liquid containing Hochest333258, uniformly mixing, then standing for a period of time, abandoning a cell culture medium, washing off residual culture medium with PBS buffer liquid, then adding the PBS buffer liquid, finally detecting a fluorescent value, and judging cell proliferation level or survival rate by virtue of fluorescence signal intensity. The method disclosed by the invention is simple and is high in repeatability and accuracy; and the proliferation level of the tumor cells can be effectively detected.

In marking execution mode, after initialization, a scanning control signal based on predetermined marking data and condition data is sent to a scanning head (20) so that a beam spot of a YAG laser beam scans spirally on the interior of a predetermined first black cell (CEB) in a two-dimensional bar code marking region on the surface of a workpiece (W). After the completion of the spiral scanning on the interior of the first black cell, the beam spot skips over from the scanning end point to a scanning start point of a second black cell (CEB) adjacent to that end point. Then, the interior of the second black cell is also scanned with the beam spot in a unit plotting pattern similar to the above. Afterward, the same spiral scanning as the above is iteratively made on a third and all subsequent black cells (CEB). Upon the completion of the spiral scanning on the last black cell, all the marking actions are complete.

The invention discloses a free cell marking and separating probe as well as a related product and application thereof, and particularly aims at fetal trophoblast cells or epithelial tissue-derived tumor cells. A free cell capture probe comprises human papilloma virus-like particles and reporter genes carried by the human papilloma virus-like particles and/or protein tag expression genes used for separating target cells. Whether a blood sample of a subject contains fetal trophoblast cells or not can be effectively detected by utilizing the characteristic that the human papilloma virus-like particles selectively infect the placental trophoblast cells, and the aim of separating the fetal trophoblast cells can be achieved after optimization. The probe can also be used for identifying or separating epithelial tissue-derived cells such as epithelial tissue-derived tumor cells in a sample by utilizing the epitheliophilic characteristic of the probe. The method is ingenious in conception, safe and reliable, and has very high sensitivity and specificity.

The invention discloses a ferrimagnetic nano material. The ferrimagnetic nano material comprises cubic ferroferric oxide nano particles and phospholipid polyethylene glycol coating the outer layers ofthe cubic ferroferric oxide nano particles. The invention also discloses an application of the ferrimagnetic nano material in magnetic particle imaging. The ferrimagnetic nano material provided by the invention can be stably dispersed in a water phase system, has the excellent magnetic particle imaging effect, and is a sensitive and efficient MPI contrast agent.

The invention discloses a marking method for microbial self-repairing of concrete cracks based on a fluorescent material. The method comprises the following steps: adding a Eu(NO3)3 solution with a certain concentration into the microbial self-repairing concrete crack, maintaining a test block at room temperature, doping eu<3+> in calcium carbonate generated by microbial mineralization and deposition in a crack area, and generating the CaCO3: Eu < 3 + > fluorescent substance which emits bright red fluorescence after being irradiated by an ultraviolet lamp in the wavelength of 365 nm. The actual position of self-repairing product calcium carbonate in the microbial self-repairing concrete test block crack can be clearly represented.