34results about How to "Valid mark" patented technology

The invention discloses the application of papaya saponin I in detecting lysosomes. In the present invention, it has been found through experiments that patricia saponin I has the effect of specifically targeting lysosomes. In order to facilitate observation and detection, the present invention further synthesizes a red fluorescent probe with papaya saponin I and a fluorescent dye, which is named as Polyphyllin I-Cy3, its structural formula is as shown in (II). The Polyphyllin I-Cy3 red fluorescent probe synthesized by the present invention can permeate the cell membrane and can be used for lysosome-specific fluorescent staining in living cells. It has good targeting, fast detection speed, good repeatability, convenient use, and no cytotoxicity, etc. advantage.

The invention belongs to the technical field of promotion of mesenchymal stem cell in-vitro osteoblast differentiation, and discloses an application of ruthenium complex modified nano selenium in promotion of mesenchymal stem cell in-vitro osteoblast differentiation. The invention provides the application of the ruthenium complex modified nano selenium in promotion of mesenchymal stem cell in-vitro osteoblast differentiation. The nano selenium can promote the mesenchymal stem cell in-vitro osteoblast differentiation of human umbilical cord, the differentiation balance is exsited between the mesenchymal stem cell ossification differentiation and esterification differentiation, so that the nano selenium has the effect for inhibiting esterification differentiation of mesenchymal stem cells, and can effectively labeling the mesenchymal stem cell. According to the invention, a ruthenium complex is used for modifying nano selenium, other auxiliary reagents are required for adding during a preparation process, the products system is simple, and the product can be directly preserved and used. The employed modification agent can greatly reduce the cytotoxicity of the ruthenium complex modified nano selenium, usage amount of the medicine for cells can be increased, external discharge is reduced, and the medicine in the cell can keep as a high level.

The invention relates to an aminoacyl-tRNA (transfer ribonucleic acid) synthetase mutant containing an amino acid sequence selected from groups consisting of amino acids shown by SEQ ID NO:4 and conservative variants thereof. The invention provides a CpK (short for N<epsilon>-(1-methylcyclopropyl-2-acrylamide)-lysine) translation system for fixed point specific insertion of N<epsilon>-(1-methylcyclopropyl-2-acrylamide)-lysine in a target protein through pairing of orthogonal tRNA and orthogonal aminoacyl-tRNA synthetase, and a method for fixed point specific insertion of CpK in the target protein by using the translation system. The CpK translation system comprises (i) N<epsilon>-(1-methylcyclopropyl-2-acrylamide)-lysine, (ii) the orthogonal aminoacyl-tRNA synthetase, (iii) the orthogonal tRNA and (iv) a nucleic acid for coding the target protein, wherein the orthogonal aminoacyl-tRNA synthetase is used for realizing aminoacylation of the orthogonal tRNA preferentially by using CpK, and the nucleic acid contains at least one selective codon specifically identified by the orthogonal tRNA. The invention also relates to an application of a mutant protein for fixed point specific insertion of CpK in protein labeling through a light click reaction with a tetrazole compound.

The invention discloses fluorescence amino carbon quantum dots, and a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing aqua ammonia with purified xylan, and stirring until the xylan is dispersed in the solution; (2) transferring the mixture into a reaction kettle, putting in a sealed muffle furnace, and carrying out hydrothermal treatment; (3) cooling the product to room temperature, and carrying out ultrasonic dispersion treatment; (4) carrying out refrigerated centrifugal separation on the treated product, removing the precipitate, and collecting the supernate to obtain a fluorescence amino carbon quantum dot water solution; and (5) concentrating the product water solution by rotary evaporation, and finally, carrying out vacuum drying to obtain the fluorescence amino carbon quantum dots. The amino carbon quantum dots have the advantages of high fluorescence, high stability, low cytotoxicity and favorable biocompatibility, and can be widely used in the fields of cell imaging, disease diagnosis, drug transfer and the like.

The invention provides an acrylyl lysine translation system which dopes acrylyl lysine into target protein by using an orthogonal tRNA, an orthogonal acrylyl lysine aminoacyl tRNA synthetase and their combination, and also provides a method for specifically doping acrylyl lysine into target protein at a fixed point by using the translation system. The acrylyl lysine translation system comprises: (i) acrylyl lysine; (ii) the orthogonal acrylyl lysine aminoacyl tRNA synthetase; (iii) the orthogonal tRNA, wherein the orthogonal acrylyl lysine aminoacyl tRNA synthetase is used for preferential aminoacylation of the orthogonal tRNA by the acrylyl lysine; and (iv) nucleic acid coding the target protein, wherein the nucleic acid contains at least one selection codon specifically recognized by the orthogonal tRNA. The invention also relates to an application of the mutant protein doped with the acrylyl lysine.

The invention relates to a video marking method and device, and the method comprises the steps: coding a mark, wherein the mark is corresponding to the description information and position of the video; converting the coded mark into an audio file; and inserting the audio file into the video according to the position. The technology, provided by the invention, based on sound coding can effectively mark the video, and the marked video just has one file, thereby preventing information file loss from causing no video marking, no arrangement method, and query and / or obtaining problems. Moreover, the marked video is high in continuity, and the mark of the video file cannot be affected by new cutting, editing or deleting of a part of video contents.

The invention discloses a biomarker for the esophagus cancer and an application of the biomarker. The biomarker is a mesoderm specific transcription factor, the amino acid sequence of the biomarker isshown in SEQ ID NO:1, the biomarker used for diagnosis of metastasis of the esophagus cancer as well as animation prognosis, the mesoderm specific transcription factor is used to prepare an agent and / or medicine for assisted diagnosis of the esophagus cancer, evaluation of the therapeutic effect and monitoring of metastasis and relapse, has an obvious effect, and is good in specificity.

The invention discloses a wireless sensor network variational Bayesian expectation maximization positioning method and system. The method includes: positioning a wireless sensor network containing holes to obtain positioning information; determining the location on the boundary of the hole according to the positioning information node; adopt multi-hop mode to obtain the distance information between each of the nodes; determine the knuckle information of each of the nodes; according to the distance information and the knuckle information, establish a maximum likelihood estimation model with hidden variables; according to the The above maximum likelihood estimation model establishes a variational Bayesian expectation maximization positioning model; updates the parameters of the variational Bayesian expectation maximization positioning model, adjusts the estimated position of the unknown node, and after multiple iterations, the unknown node is obtained location information. The positioning accuracy of the wireless sensor network can be improved by adopting the invention.

System of the assets identification 0nd protection which is bound to marking 0nd reverse identification of stolen things or lost transport means, things and animals by the means of electronic records, in order to determinate correctly the origin and the owner. It consists of protection elements (microdots and microchips bearing unique codes), registered in the database of protected property of the central register, which is connected to the terminals of authorized persons (clients with the access to the central register via Internet) and of the protection elements database of the central register, which is connected to the set of registered terminals places. Each terminal, in the set of terminals of authorized persons, is equipped by the readers of protection elements. Each terminal in the set of registration places is equipped by applicators of the protection elements. All protection elements, used in the identification sets and placed on registered protected property, are registered in the central register of the system for the assets identification and protection.