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High-fluorescence quantum efficiency red-light polymer, quantum dot solution and application

A quantum dot solution, fluorescent quantum technology, applied in the direction of fluorescence/phosphorescence, measuring devices, material analysis through optical means, etc., can solve the problems of complex design process, difficult synthesis and preparation, etc., and achieve little effect on cell activity and good biophase capacitive effect

Active Publication Date: 2019-04-05
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complex structure design process and the difficulty in synthesis and preparation of semiconducting polymer quantum dots emitting near-infrared fluorescence, there have been few reports in recent years, especially semiconducting polymers whose emission wavelength is within the first optical window (650-900nm) of biological tissues. quantum dot

Method used

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  • High-fluorescence quantum efficiency red-light polymer, quantum dot solution and application
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  • High-fluorescence quantum efficiency red-light polymer, quantum dot solution and application

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Embodiment 1

[0032] A method for preparing a red photopolymer with high fluorescent quantum efficiency, comprising the steps of:

[0033] 1. Preparation steps of monomer M1:

[0034] (1) A1 (2g, 6.8mmol), thiophene borate (3.57g, 17mmol), NaHCO 3 (5.72g, 68mmol), tris(dibenzylideneacetone)dipalladium (0.31g, 0.34mmol), tris(o-methylphenyl)phosphine (0.41g, 1.36mmol) were added to tetrahydrofuran (80mL) and water ( 30mL) of the mixed solution, repeated degassing, in N 2 Under the atmosphere, the temperature was raised to 90°C for 72h.

[0035] The reaction solution was cooled to room temperature, extracted with chloroform and saturated brine, the organic phase was collected, dried by adding anhydrous magnesium sulfate, and the solvent was spin-dried. After passing through a silica gel column, the crude product was recrystallized from a mixed solvent of dichloromethane and petroleum ether to obtain an orange-red solid B1 (1.2 g, 60%).

[0036] (2) Add B1 (130mg, 0.43mmol) and N-bromosucc...

Embodiment 2

[0060] A red photopolymer quantum dot solution with high fluorescent quantum efficiency is prepared by the following method: the red photopolymer with high fluorescent quantum efficiency shown in formula I is dissolved in tetrahydrofuran to form a solution with a concentration of 0.5mg / mL The solution is solution one; polystyrene-maleic anhydride with a number average molecular weight of 1.7kg / mol is dissolved in tetrahydrofuran to make a solution with a concentration of 0.5mg / mL as solution two; the volume ratio is 5:1 Ratio, mix solution 1 and solution 2 to obtain a precursor solution, inject the precursor solution into deionized water with a volume ratio of 3:10 under ultrasonic conditions; ), at N 2 Heated to 90°C under protection (the experiment proved that the temperature can be 90-95°C), evaporated to remove tetrahydrofuran, and stopped N 2 , stop heating, lower to room temperature, pass through a 220nm water filter head, and the filtrate is a red photopolymer quantum ...

Embodiment 3

[0065] Biosynthesis of Red Photopolymer Quantum Dots with High Fluorescence Quantum Efficiency.

[0066] Add 20 μL of 5% weight / volume polyethylene glycol aqueous solution (weight average molecular weight = 3.35 kg / mol) and 20 μL of HEPES (hydroxyethylpiperazine ethylsulfuric acid) buffer (1 M, pH = 7.2) to 1 mL Streptavidin (1 mg / mL, 60 μL) was added to the 50 μg / mL aqueous solution of red photopolymer quantum dots with high fluorescence quantum efficiency, and mixed. Add 20 μL of freshly prepared EDC (1-ethyl 3-[3-dimethylaminopropyl]carbodiimide hydrochloride) solution (5 mg / mL in Milli-Q water), mix, and dissolve the above mixture Stir at room temperature for 4 hours. Centrifuge with an ultrafiltration centrifuge tube (molecular weight cut-off = 100,000) to obtain streptavidin-biochemical red photopolymer quantum dots.

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Abstract

The invention discloses a high-fluorescence quantum efficiency red-light polymer, quantum dot solution and an application. A structural formula of the high-fluorescence quantum efficiency red-light polymer is as shown in the specification, and the weight-average molecular weight of the polymer is 35kg / mol. The high-fluorescence quantum efficiency red-light polymer transmits fluorescence in a deepand near infrared area (650nm-900nm), a high-fluorescence quantum efficiency red-light polymer quantum dot with particle diameter smaller than 20nm is prepared from the polymer by a nano re-precipitation method, and a cell micro-tubular protein structure is successfully and effectively marked. A cytotoxicity test proves that the high-fluorescence quantum efficiency red-light polymer quantum dot has low influence on cell activity and good biocompatibility and provided with a near infrared fluorescence probe for in-vivo imaging.

Description

technical field [0001] The invention belongs to the field of polymer preparation, and in particular relates to a red light polymer with high fluorescence quantum efficiency, quantum dot solution and application thereof. Background technique [0002] As a hotspot in the field of emerging materials, organic polymers have attracted extensive attention from many researchers. This is mainly due to the fact that the polymer backbone composed of long repeating units can achieve good energy transfer, so that it has strong light-harvesting ability and optical signal amplification effect. Compared with most small molecular compounds, organic polymers exhibit better optical properties, cell compatibility, low toxicity and easy modification, which make them widely used in light-emitting materials, chemical biosensing and imaging, etc. field. [0003] In the research of cell biology, in order to observe structures and molecules of interest, the most common method is to label them with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G61/12G01N21/64
CPCC08G61/126C08G2261/122C08G2261/1412C08G2261/1646C08G2261/19C08G2261/3142C08G2261/3223C08G2261/3246C08G2261/411C08G2261/522G01N21/6428G01N2021/6439
Inventor 陈于蓝严春梅
Owner TIANJIN UNIV
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