Application and method of fluorine oil as solvent for reducing water sensitivity of liquid phase biological sample for terahertz wave detection
A biological sample, water-sensitivity technology, applied in the field of medical testing, can solve the problems of insufficient water sensitivity, strong absorption of THz waves, and limited effect.
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Embodiment 1
[0036] Example 1. Optimization of conditions for flushing biological samples with fluorine oil to replace surrounding water molecules
[0037] The condition optimization method for flushing biological samples with fluorine oil to replace surrounding water molecules, the specific steps are as follows:
[0038] (1) Taking tumor cells as the biological material model, the concentration of 5ml was 1.5×10 6 The cell / ml breast cancer MDA-MB-231 cell suspension was dropped on polyethylene (PE) sheets to make single-layer adherent cell slides;
[0039] (2) Clamp the cell slide into a detection fixture with a sample inlet and a sample outlet (the liquid sample detection pool of Bruker, Germany, model: A145, purchase route: Bruker), from the inlet to the outlet Fluorine oil was injected into the microfluidic syringe pump, the PE sheet was flushed at a flow rate of 20 μl / min, and the THz spectrum detection results of the sample were recorded every 1 min.
[0040] Test results such as ...
Embodiment 2
[0041] Example 2, using fluorine oil as a solvent to detect tumor cells
[0042] The method of using fluorine oil as a solvent to detect tumor cells, the specific steps are as follows:
[0043] (1) The concentration of 5ml is 1.5×10 6 The cell / ml breast cancer MDA-MB-231 cell suspension was dropped on a polyethylene (PE) sheet to make a single-layer adherent cell slide;
[0044] (2) Clamp the cell slide into a detection jig with a sample inlet and a sample outlet (same as Example 1). Experimental group: Inject fluorine oil from the injection hole with a micro-flow syringe pump to wash the PE sheet with or without adherent cells at a flow rate of 20 μl / min for at least 5 minutes; Wash the PE sheet with or without adherent cells at a flow rate of 20 μl / min for at least 5 minutes;
[0045] (3) Close the sample inlet and sample outlet of the fixture, and perform THz spectrum detection.
[0046] Test results such as Figure 5 shown. The results showed that when the cells were...
Embodiment 3
[0047] Embodiment 3, utilize fluorine oil as solvent detection bacterium
[0048] The method of using fluorine oil as a solvent to detect bacteria, the specific steps are as follows:
[0049] (1) Pick a single bacterial colony on the blood plate after pure culture and apply it evenly on a polyethylene (PE) sheet by the glass slide spin coating method to make a bacterial smear;
[0050] (2) Clamp the bacterial smear into a detection fixture (same as Example 1) with a sampling hole and a sampling hole, experimental group: inject fluorine oil from the sampling hole with a micro-fluid injection pump to have bacteria Wash the smears with a flow rate of 20 μl / min for at least 5 minutes; control group: inject PBS buffer solution from the injection hole with a microfluidic syringe pump, wash the smears without bacteria at a flow rate of 20 μl / min for at least 5 minutes;
[0051] (3) Close the sample inlet and sample outlet of the fixture, and perform THz spectrum detection.
[0052]...
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