160results about How to "Little effect on activity" patented technology

The invention discloses a catalysts grading method for coker dry gas hydrogenation for preparation of ethylene cracking feed. According to the method, coker dry gas is contacted with a hydrogenation catalyst to carry out a hydrogenation reaction, and olefin in coker dry gas is saturated to further obtain an ethylene cracking feedstock, wherein three hydrogenation reaction zones are successively arranged in a hydrogenation reactor. By reasonable grading of catalysts in different systems, advantages of the respective catalysts during different hydrogenation processes are fully performed. By synergism among the catalysts and by cooperation of reaction temperature gradients of the three hydrogenation reaction zones, activity of the monolithic catalyst is raised.

The invention relates to a preparation method and application of a magnetic silicon dioxide composite microsphere. Superparamagnetic ferroferric oxide nano particles of which the diameter ranges from 4 nm to 30 nm are prepared through a high-temperature pyrolysis method, a silicon dioxide shell of which the thickness ranges from 5 nm to 20nm covers the outer surfaces of the magnetic ferroferric oxide nano particles through a reverse microemulsion method, amination modification is conducted on the silicon dioxide surface, glutaraldehyde is used as a crosslinking arm, ligand protein is connected into, and protein separation is conducted through specific binding of ligand protein and target protein. The prepared magnetic microsphere is small in particle diameter and good in monodispersity, the composite microsphere with amine is large in specific surface area, nucleophilic addition is utilized, after the crosslinking arm glutaraldehyde is connected into, multiple kinds of ligand protein can be connected into, and then multiple kinds of target protein can be separated. The method is suitable for rapid separation and application of protein in biological samples, and has wide application prospect and great application value in the biomedical field and other fields.

The invention provides a micro-fluidic chip capable of being used for single particle screening and forming drop wrappage for exporting. The micro-fluidic chip comprises at least one sample channel and at least one oil reservoir. The micro-fluidic chip is connected with a liquid sample introduction device to form a micro-fluidic chip device for forming a single particle wrapped drop, and the micro-fluidic chip device and a particle capture device can further form a microfluidic operating system capable of being used for forming the single particle wrapped drop. The invention further provides amethod of forming and separately exporting the target single particle wrapped drop in the micro-fluidic chip.

The invention discloses a heavy metal biological adsorbent using an eggshell membrane as a matrix and a preparation method thereof. The heavy metal biological adsorbent adopts chitosan as an immobilized carrier and eggshell membrane powder as a basis material, and comprises the following components in part by weight: 10 to 13 parts of the eggshell membrane powder, 20 to 30 parts of the chitosan of which the deacetylation degree is 86.9 percent, and a minute amount of gelatinous substance of a complex which contains sodium and potassium and is formed in the preparation process. The particle size of the biological adsorbent is 2 to 3 millimeters. The method comprises the steps of performing vacuum freeze drying and mechanical disintegration on a fresh eggshell membrane (a fibrous membrane between an eggshell and egg white), and then embedding the eggshell membrane with chitosan glue solution to obtain the biological adsorbent for adsorbing heavy metals. The biological adsorbent can be used for treating waste water containing the heavy metals of nickel, cobalt, cadmium, copper, lead and the like of which the concentration is lower than 100 mg / L, and the adsorption rate achieves 42 to 98 percent.

A process for extracting tea polyase from the leftover generated by extracting tea polyphenol includes extracting toxic theocin and most of protein by chloroform and drying. Its advantage is high extracting rate (40% or more).

The invention relates to a method to detect the activity of alga cells by means of neutral red staining, which comprises centrifugation of the solution containing alga to be measured; cleaning the solution with distilled water to get the alga cell precipitate; adding neutral red Ringer solution of 0.15mg / ml to 0.20mg / ml to stain the alga cell precipitate for ten to fifteen minutes to get cell suspension; detecting the stained cell number in the cell suspension under ordinary biomicroscopes; numeration of twenty visual fields for each sample, and calculating the cell death rate by using the formula. In the formula, r represents the cell death rate; A represents the number of unstained cells; B represents the number of cells stained red. The method overwhelms the defect of the prior art, and is a convenient and quick method to detect the activity of the frustules by means of neutral red staining.

The invention discloses a biosorbent removing lead ions in wastewater and preparation, application and regeneration methods thereof, the biosorbent is granular and uses calcium alginate and gelatin as carriers in which grapefruit peel powder is embedded, mass ratio of the calcium alginate, the gelatin to the grapefruit peel powder is (8-10):(4-5):1, the biosorbent is prepared by using mixed glue of the calcium alginate and the gelatin to cover the grapefruit peel powder to result in a sorbent embedding body, instilling the sorbent embedding body into CaCl2 solution for curing and washing the body with de-ionized water, and implementing freeze drying. The biosorbent of the invention is added into wastewater for proceeding adsorption treatment for at least 30 minutes at normal temperature in which pH value is 3.5-7.0, which can basically remove lead ions in the wastewater. The biosorbent of the invention has the advantages of abundant sources of raw materials, low cost, high mechanical strength and excellent adsorption effect on the lead ions in the wastewater.

The invention discloses an Ag / g-C3N4 / TiO2 / AC catalyst as well as a preparation method and application of the catalyst. The Ag / g-C3N4 / TiO2 / AC catalyst is prepared by adopting tetrabutyl titanate, melamine, activated carbon, silver nitrate and the like as main raw materials in a way of controlling the hydrolysis reaction, polycondensation reaction and photochemical reaction. The catalyst is not only wide in spectral response, but also has a thermocatalytic function, and has a good effect on degrading of volatile organic pollution gases; moreover, the humidity of the gas has slight impact on the activity. The Ag / g-C3N4 / TiO2 / AC catalyst has a specific advantage of degrading damp and hot organic pollution gases released from factory lines.

The invention provides a method for preparing a solid enzyme reagent which is used to fast measure the organophosphorus and carbamate toxicity of pesticide residue and its using method. The agent is formed by choline esterase, buffer solution and carrier. The solid enzyme reagent can be stored at indoor temperature above 20 days.

The invention belongs to the field of deep processing of garlic, and particularly discloses a method for purifying alliinase by double-water-phase separation. The method comprises the following steps: (1) peeling and precooling garlic squamous bulbs, immersing in a precooled extract, homogenizing, centrifuging, and discarding garlic slag, thereby obtaining the supernate crude enzyme solution; (2) dissolving ammonium sulfate, sodium dihydrogen phosphate or trisodium citrate in a Britton-Robinson buffer solution, oscillating to completely dissolve, adding a PEG (polyethylene glycol) solution, evenly mixing by oscillation, and standing for phase splitting, thereby obtaining a double-water-phase extractant; (3) adding the crude enzyme solution into the double-water-phase extractant, oscillating, centrifuging, and standing for phase splitting; and (4) ultrafiltering the lower phase of the enriched alliinase, and carrying out vacuum freeze drying on the trapped fluid to obtain the alliinase solid. The method is simple to operate, has the advantages of simple apparatuses, time saving, low cost, and higher alliinase purification multiple and enzyme activity recovery rate than the prior art, and can easily implement scale-up production.

The invention belongs to the field of environmental protection and particularly relates to an advanced sewage treatment technology which comprises the following steps: after being subjected to biochemical treatment, sewage sequentially passes through a preoxidation unit, an electrolysis catalytic oxidation unit, a regeneration unit, a catalyst ultrasonic cleaning unit and a catalyst separating unit to meet the discharge standard. The static bed catalytic oxidation technology is adopted to reduce the COD load to a certain level, so that the follow-up COD load of a subsequent electrolysis catalytic oxidation unit is also reduced, the improvement on the sewage treatment depth is facilitated, and the effluent quality is better and more stable. With a static bed catalytic oxidation unit arranged in front, heavy metals and other materials in water toxic to a catalyst can be intercepted, so that a suspended particle catalyst with higher production cost can be protected, and the service life of the catalyst is prolonged. By timely adjusting the dosage of an oxidizing agent, the problem about load fluctuation can be instantly solved, the problem that the suspended particle catalyst is attached by dirt is also solved, the recycling of the suspended particle catalyst is realized, and the cost is lower.

The invention discloses a chocolate microbacterium magnetic cell as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, preparing a Fe3O4 magnetic nano material wrapped by chitosan, then preparing the cell suspension of a chocolate microbacterium, finally adding the obtained Fe3O4 magnetic nano material wrapped by the chitosan into the cell suspension of the chocolate microbacterium, controlling the temperature to below 30 DEG C, and stirring and adsorbing for 10-600 min, so that the chocolate microbacterium magnetic cell is obtained. The chocolate microbacterium magnetic cell is used for catalyzing asymmetric hydrolysis reaction of meso dimethyl ester to generate (4S and 5 R) semi-ester, and can be recycled. After the chocolate microbacterium magnetic cell is recycled for 10 times, the catalytic conversion rate of the chocolate microbacterium magnetic cell for the substrate biotin dimethyl ester is still more than 70 percent. The chocolate microbacterium magnetic cell has the advantages of simple preparation method, high recovery rate of enzyme activity and good stability, and can be conveniently and quickly recycled and reused in a magnetic field.

The invention is about the process to produce the chitose. The process is : first to dissolve the chitosan in the acid and add into the H2O2 at 50-80deg.C for 2h, next to add into the alkali to adjust pH7-10 for 1-3h, last to add into the ethanol of 3-10 times volumes to deposit the chitose. The process has the good efficiency and short reaction time.

The invention is a method of making microencapsulated functional cell. It mixes evenly the filtered and axenic bubble enveloping liquid and good cells in proportion, then places the mixture in the multichannel generating equipment, spray the cell suspended liquid to form bubble, and then processes and reserves. The generating equipment includes injection pump, connecting hose (2-1), union joint, hose core, gland, sealing ring, fixing ring, jacket and compressed gas generator.

The present invention is process of preparing immobilized microbe and relates to microbe immobilizing method. The immobilizing process of the present invention adopts PVA and sodium alginate as the embedding material and sodium sulfate to replace boric acid, and includes a two-step immobilizing reaction with calcium chloride and sodium sulfate. The prepared immobilized microbe has no adhesion, less influence of the reaction reagents and reaction conditions on the activity of cell, no toxicity, simple operation, high strength and high stability. The preparation process may be performed continuously, and possesses excellent application foreground in biological waste water treatment, biomedicine preparation, food fermentation and other fields.

The invention belongs to biological technique field, relates to small molecular inhibitor against staphylococci tryptophanyl-tRNA synthetase (WRS), molecular structure of the inhibitor shown in formula I, method of the inhibitor as medicament for preventive or treating relevant bacterial disease. Biochemical and biological experiments have shown that the inhibitor has strong binding force with target protein staphylococcus epidermidis or Staphylococcus aureus tryptophanyl-tRNA synthetase, can inhibit the activity of the synthetase, inhibit the growth of staphylococcus epidermidis Staphylococcus aureus. It has no toxicity to mammalian cell. The inventive inhibitor can be used for preparing medicament for treating disease caused by staphylococci, and preparing into disinfectant liquid.

The invention provides a compound fungicide comprising Trichoderma asperellum spore powder of and application thereof. The fungicide comprises the following components byweight: aculeatus, 2-30% of Trichoderma asperellum spore powder, 5-40% of a chemical fungicide, 5-10% of a surfactant and the balance of a carrier. The chemical fungicide is one of phenamacril, picoxystrobin, dimethomorph, cyazofamid, fluopicolide, hymexazol, propamocarb hydrochloride and thifluzamide; and the a surfactant comprises a dispersant and an active agent. The fungicide of the invention has the advantages of quick result, long duration and small amount, can effectively control soil-borne diseases of crops; and chemical fungicide and the surfactant in the product are repeatedly screened and have little influence on the activity of Trichoderma asperellum spore powder.

The invention relates to a microfluidic chip and a microfluidic cell sorting system. A cell sample sequentially passes through a liquid flow gathering area, an optical detection area and a jet flow sorting area on the microfluidic chip, and then is sorted to obtain target cells; and the jet flow sorting area comprises at least one jet flow pool, at least one surface of the jet flow pool is of a membrane structure, the volume of the jet flow pool is changed by the change of the membrane structure, jet flow is generated, and the jet flow impacts the cells to enter different outlets. Meanwhile, the microfluidic cell sorting system provided by the invention comprises the microfluidic chip, a sample injection system, an optical detection system, a jet flow sorting system and a control system. The technology provided by the invention has small influence on cell activity, the microfluidic chip can be replaced, and cross contamination is not generated; and the microfluidic chip adopts a two-layer design, the processing is simple and cheap, the jet flow pool and the membrane structure are directly made of materials such as glass forming the microfluidic chip, and when the processing of themicrofluidic chip is completed, the jet flow sorting structure is completed, the membrane structure does not need to be additionally processed, and the process is simpler.

Disclosed are a preparation method for hepatic receptor imaging agent 99mTc-galactosyl-human serum albumin fusion interferon and the application of the albumin fusion interferon, belonging to the nuclear medicine technical field. The invention adopts a stannous reduction method for the radioactive technetium tagging of the hepatic receptor imaging agent 99mTc-galactosyl-human serum albumin fusion interferon GHSA-IFN; upon polyamide-66 thin layer chromatography analysis, the radiochemical purity of the prepared 99mTc-GHSA-IFN is more than 90% and can be stabilized for more than 6 hours at room temperature, in line with the requirements of scientific research and clinical use. The distribution in mice and the imaging results of rats show that the 99mTc-GHSA-IFN can be incepted by liver in a specific way, and the liver imaging is very clear within 10-30 minutes which is the best time for liver imaging. Therefore, the 99mTc-GHSA-IFN is a good new liver receptor function SPECT imaging agent and can be used to evaluate the liver receptor functions, diagnose liver diseases and evaluate the liver disease treatment drug efficacy; at the same time, the 99mTc-GHSA-IFN is also used to prepare liver targeted therapy drugs and the treatment drugs for viral hepatitis.

The invention discloses a method for preparing recombinant heparinase III by utilizing an SUMO fusion expression system and the heparinase III prepared by the method, the preparation method comprises the following steps: selecting a heparinase III sequence from Pedobacter heparinus, wherein the amino acid sequence of the heparinase III is shown as SEQ ID No.1, and the total number is 659aa; removing a signal peptide sequence to obtain a DNA sequence of the heparinase III, such as SEQ ID No.2; inserting the DNA sequence of the heparinase III into a pSMART vector plasmid with an N-terminal SUMO protein tag; transforming the correct plasmids into BL21 (DE3) escherichia coli competent cells, selecting monoclone, and obtaining fusion protein through fermentation and purification; and cutting the SUMO tag protein from the fusion protein by using the SUMO protease to obtain the heparinase III. The method has the advantages that the solubility of the target protein is improved, correct folding of the target protein is promoted, and inclusion bodies are prevented from being formed; the purification cost is reduced and the purification efficiency is improved; the molecular weight of the SUMO protein tag is small, the influence on the enzymatic activity of the heparinase III is small, and the purified heparinase III is obtained.

The invention relates to a rapid influenza virus labeling and tracing method based on aggregation-induced emission molecules. According to the method, the aggregation-induced emission molecules are used as fluorescent labels, and the labeling of influenza viruses by the aggregation-induced emission molecules is realized through electrostatic interaction. Through a laser confocal imaging analysis system, the whole process tracing of an infection process of infecting host cells with the influenza viruses can be realized. The labeling method is simple and efficient, has little influence on virusactivity, and can fully reflect a group dynamic infection behavior of the viruses in a single cell. By combining excellent optical properties of the aggregation-induced emission molecules, a multi-objective, real-time and long-time influenza virus tracing method is established, and a powerful tool is provided for globally and efficiently researching an influenza virus infection mechanism.

The invention belongs to the field of processing agricultural products, and particularly relates to a preparation method of dioscorea opposita thunb slime polysaccharide and resistant starch. The preparation method of dioscorea opposita thunb slime polysaccharide and resistant starch comprises the following steps: washing and peeling fresh dioscorea opposita thumb, protecting color, slicing, crushing, homogenizing, putting homogenate into a high-pressure pulsed electric field for extraction, adding water into the treated dioscorea opposita thumb homogenate and performing high-speed centrifugation, and centrifuging starch residues at the lower layer for three times; respectively collecting supernatant and lower-layer starch residues obtained through repeated centrifugation; filtering the centrifuged supernatant through a molecularly imprinted membrane and eluting with water, to obtain a dioscorea opposita thumb slime polysaccharide solution, concentrating and drying to obtain dioscoreaopposita thumb slime polysaccharide, adding water into the lower-layer starch residues and performing membrane filtration, preparing a starch emulsion after filtering, performing pressure-heating treatment in an autoclave and then adding pancreatic amylase and pullulanase for enzymolysis, performing enzyme denaturing and performing refrigeration and ageing, hot-air drying, pulverizing and screening to obtain the dioscorea opposita thumb resistant starch.

The present invention provides an electromagnetic stirring separation device for biopharmacy. The device includes an operation surface, a body, a stirring base and a support bar. The outer surface of the operation surface comprises a display screen, a switch and an automatic switch; a support plate is fixedly mounted on the upper end face of the body; the body is internally provided with a cavity; the cavity is internally provided with a control unit, a variable-frequency power source and a sensor; and the control unit includes a printed circuit board and an A / D converter welded to the printed circuit board, an embedded central processing unit, a storage, a D / A converter, a relay and a battery. Compared with the prior art, the device provided by the present invention has the following beneficial effects: the device prevents inadvertent tough of switch by the staff in case of an accident leading to accidental opening of the device, so as to enhance the use safety; the device can be automatically controlled, and has the advantages of minimal impact on the pharmaceutically activity, high separation efficiency, easiness to use and operation, good stability and high reliability.

The invention relates to a preparation method and an application of a galactosyl proserum fused interferon, belonging to the targeted biological medicine technical field. The invention takes a proserum fused interferon as a galactosyl precursor, can provide enough sites for glycosylation, and then more effectively prepare the galactosyl proserum fused interferon with different sugar densities (between 7 and 35). Compared with the proserum fused interferon, the compound has obvious liver targeting character and the same pharmacological activity, is hopeful to be used for preparing a novel virus hepatitis targeted therapeutic medicine, obviously improves the medicine concentration of IFN in the liver, prolongs the biological half-life of the galactosyl proserum fused interferon, effectively reduces the dosage of the clinic IFN, eases the pain of patients due to repeated injection, reduces generation of side reaction, greatly reduces the treatment cost of the patients, improves the treatment level of virus hepatitis in China, promotes excellent health of Chinese people, and has good social benefit and significant economic benefit.

The invention discloses a polypeptide for recognizing immune cells. The polypeptide comprises the following amino acid sequences: (a) an amino acid sequence of a C-terminal fragment sequence EQPDPGAVAAAAILRAILE containing human Triokinase / FMN cyclase; or (b) an amino acid sequence which is basically the same as the amino acid sequence in (a), and the basically the same means that the amino acid sequence in (a) has more than 80% of identity with that in (b). The invention also discloses a nucleic acid sequence for encoding the polypeptide; the polypeptide for targeted recognition of immune cells comprising the polypeptide and a polypeptide probe with a reporter group; a kit comprising the probe; and an application of the polypeptide or the probe in preparation of the kit for imaging livingcells of mammals.

The invention provides an antibody conjugate and a method thereof, and the antibody conjugate comprises: (1) an activation buffer which is a 0.05 M MES buffer with a pH of 5.0-6.0; (2) a reaction buffer which is a 0.04 M phosphate buffer with a pH of 7.0-8.5; (3) a stopping solution which comprises a 0.04 M phosphate buffer with a pH of 7.0-8.5, and 0.04 M Gly; (4) a blocking solution which comprises a 0.04 M phosphate buffer with a pH of 7.0-8.5, and a BSA solution with a mass fraction of 0.01%-1%; (5) a preservation solution which is a 0.02 M Tris buffer with a pH of 7.0-7.8; (6) a reaction buffer which comprises a 0.02 M phosphate buffer with a pH of 7.0-7.5, 0.15 M sodium chloride, and sodium dodecyl benzene sulfonate with a mass fraction of 0.005%-0.5%; (7) latex which is latex particles with carboxyls.

Disclosed is a polypeptide that recognizes immune cells, the polypeptide comprising the following amino acid sequence: (a) an amino acid sequence containing C terminal fragment sequence EQPDPGAVAAAAILRAILE of human Triokinase / FMN cyclase and the homologous sequence thereof; or (b) an amino acid sequence in which the amino acid sequence is substantially the same as described in (a), the substantially the same referred to amino acid sequence having at least 80% sequence identity to the amino acid sequence as described above in (a). Also disclosed in the present invention are a nucleotide sequence encoding the polypeptide; a polypeptide probe that targets recognized immune cells, comprises the polypeptide as described above and has a reporter group; a kit comprising the above-mentioned probe;and the use of the polypeptide or probe as described above in the preparation of the kit for mammalian living cell imaging.

The invention discloses polypeptide for target identification of immunocyte. The polypeptide comprises following amino acid sequences: (a) an amino acid sequence of a C terminal fragment sequence AILEVLQS containing human Triokinase / FMN cyclase of and a homologous sequence of the amino acid sequence; or (b) an amino acid sequence which is basically the same as the amino acid sequence as shown in (a), wherein the basic same meaning lies in that the amino acid sequence in (a) has 70% or above of sequence identity. The invention further discloses a nucleic acid sequence for coding the polypeptide, a polypeptide probe for target identification of immunocyte, containing the polypeptide and with a reported group, and a reagent kit comprising the probe, and applications of the polypeptide or theprobe.

The invention discloses a preparation method for encapsulating SOD drug carrier with new microsphere biological materials, which comprises the preparation of blank chitosan microspheres, the preparation of SOD chitosan microspheres, the drug loading capacity of SOD chitosan microspheres and the encapsulation thereof. The determination of sealing rate and the in vitro drug release test of SOD chitosan microspheres completed its preparation; the surface of the SOD chitosan microspheres was observed under an optical microscope to be smooth and uniform, good dispersibility in normal saline, and a particle size range of 1.2~3.8um ; The reaction conditions of the preparation process of SOD chitosan microspheres are mild, no organic solvent is needed, the pH is close to neutral, and the activity loss of SOD is small; the average value of the samples to be tested is 0.3, and the SOD content in chitosan microspheres is 5KU=10mg or 5.31× 10 4 U / g, encapsulation rate 97.3%; The inventive method is suitable for the mass production of SOD health products, beauty enzyme preparation SOD cosmetics in China, and also provides a new technical method for the production and application of SOD medicinal enzyme preparation.

The invention relates to application and a method of fluorine oil as a solvent for reducing the water sensitivity of a liquid phase biological sample for terahertz wave detection, by utilization of the principle that the fluorine oil has very low absorption in THz frequency band, and can replace solvent water with very high absorption around a biological substance, the problem of the water sensitivity of the liquid phase biological sample for terahertz wave detection can be solved, the invention also discloses a method for fixing a to-be-tested substance in the to-be-tested biological sample in a detection device, using the fluorine oil for scouring the sample for replacement of water molecules around the substance, and finally performing THz spectrum detection, the method has the advantages of simple operation, can reduce the signal loss of a THz detection signal passing through the liquid sample, improves the detection sensitivity, and has important significance for the application of THz spectroscopy in the biomedical field.