4results about How to "Little effect on activity" patented technology

The application discloses a monoclonal antibody DL1 of Dielma bacteria and application thereof, wherein the monoclonal antibody DL1 comprises a DL1 heavy chain and a DL1 light chain, and the monoclonal antibody DL1 is coupled with activated carboxyl magnetic beads to obtain a magnetic bead-antibody conjugate. The magnetic bead-antibody conjugate is used for enrichment, and Dielma bacteria can be enriched to a large extent. The magnetic bead sorting enrichment of Dielma bacteria is selected by using a magnetic force frame, and the method has low cost and small influence on the activity of target bacteria compared with a chip or a flow sorting method. The enriched Dielma bacteria can be applied to the analysis of metabolites of the bacteria, and can also be applied to qualitative or quantitative analysis.

The invention relates to a gelatin-based high internal phase emulsion as well as a preparation method and application thereof. The gelatin-based high internal phase emulsion comprises a water phase and an oil phase; the water phase is prepared from the following raw materials: gelatin, water, glutaraldehyde and a disulfide bond cross-linking agent; the preparation raw materials of the oil phase comprise liquid paraffin and a compound emulsifier. The gelatin-based high-internal-phase emulsion foam material prepared from the gelatin-based high-internal-phase emulsion provided by the invention has the advantages of high porosity, high pore structure connectivity, good structural stability and support performance, good biocompatibility, realization of digestion, and small influence of the digestion process and product on cell activity.

This invention discloses an intracellular imaging system for simultaneously detecting UDG and miR-375 expression levels and its applications. The intracellular imaging system uses a tetrahedral DNA nanostructure as a carrier, loading a HAT-UDG probe with an on / off conformation, and coupling it with a split CRISPR / Cas12a detection component for recognizing miR-375. The split CRISPR / Cas12a detection component includes at least Cas12a protein, scaffold RNA, spacer RNA / equivalent split crRNA components, and a fluorescent reporter substrate. This invention is the first to perform a logical AND operation between two key indicators: UDG enzyme activity and miR-375 expression level. The system can only ultimately trigger a complete fluorescence signal when both are simultaneously highly expressed. This multi-target synergistic activation design constructs a precise molecular recognition logic gate, enabling extremely accurate differentiation between prostate cancer cells (RV-1) and normal cells (293T), as well as other cancer cells (5637) that highly express UDG, fundamentally avoiding misjudgments caused by fluctuations in a single indicator or non-specific expression, resulting in extremely high imaging specificity.

The utility model relates to a product extraction device for producing acetone through synthesis gas pressure fermentation, which comprises a synthesis gas pressure fermentation tank, a membrane filtration unit, a reduced pressure flash tank, a gas condensation pipe and an acetone storage tank, a liquid inlet of the membrane filtration unit is communicated with a fermentation liquid discharge port, and a reflux inlet of the membrane filtration unit is communicated with the fermentation tank; a liquid inlet of the reduced-pressure flash evaporation pipe is communicated with a liquid outlet of the membrane filtration unit; a gas inlet of the gas condensation pipe is communicated with a vapor phase outlet of the vacuum flash tank; and a condensate outlet of the gas condensation pipe is communicated with an acetone storage tank. According to the product extraction device, firstly, thalli of fermentation liquor are intercepted through the membrane filtration unit, the activity of the thalli is reserved, and the influence on the activity of the thalli is low; according to the invention, acetone is used as a raw material, then the reduced pressure flash tank is used for reducing the pressure of the fermentation liquid and then flash evaporation is carried out, the temperature is maintained at 60 DEG C by using fermentation waste heat, so that the acetone is rapidly vaporized to form gas-liquid two-phase flow, and the design not only improves the vaporization efficiency of the acetone, but also reduces the additional cooling requirement and reduces the energy consumption.