13results about How to "Little effect on activity" patented technology

The invention belongs to the technical field of cell surface engineering, and particularly relates to a preparation method of TA-Fe < 3 + > coated chlorella capable of improving biomass accumulation. According to the invention, chlorella cells are used as a coating object, a metal compound tannic acid-iron (III) (TA-Fe < 3 + >) is used as a coating material, and the shell has small influence on the physiological activity of the chlorella cells and has good biocompatibility. Compared with a natural chlorella cell, the surface of the chlorella cell is coated with a TA-Fe < 3 + > bionic shell, so that the viability and stability of the cell in a severe environment can be remarkably improved, the utilization rate of HCO3 <-> in the environment can be effectively improved through the synergistic effect between the shell and the cell, the biomass accumulation of the chlorella cell is further improved, and the biological activity of the chlorella cell is improved. And a new solution and possibility are provided for producing clean energy.

The invention belongs to the technical field of traditional Chinese medicines, and discloses application of pulsatilla saponin B4 in preparation of a medicine for treating intervertebral disc degeneration and discogenic pain. The pulsatilla saponin B4 is used for preparing the medicine for treating the intervertebral disc degeneration and the discogenic pain, the intervertebral disc degeneration can be treated, the discogenic pain can be relieved, and the effect is good. The pulsatilla saponin B4 disclosed by the invention is used for preparing the medicine for treating intervertebral disc degeneration and intervertebral disc-derived pain, and the pulsatilla saponin B4 has small influence on cell activity, has no cytotoxicity and is good in medicine safety.

The application discloses a monoclonal antibody DL1 of Dielma bacteria and application thereof, wherein the monoclonal antibody DL1 comprises a DL1 heavy chain and a DL1 light chain, and the monoclonal antibody DL1 is coupled with activated carboxyl magnetic beads to obtain a magnetic bead-antibody conjugate. The magnetic bead-antibody conjugate is used for enrichment, and Dielma bacteria can be enriched to a large extent. The magnetic bead sorting enrichment of Dielma bacteria is selected by using a magnetic force frame, and the method has low cost and small influence on the activity of target bacteria compared with a chip or a flow sorting method. The enriched Dielma bacteria can be applied to the analysis of metabolites of the bacteria, and can also be applied to qualitative or quantitative analysis.

The invention discloses a catalyst for preparing low-carbon olefin by Fischer-Tropsch synthesis of light oil and crude oil as well as preparation and application of the catalyst. The catalyst comprises the following components in parts by mass: 0.1-30 parts of a metal active component; 30-80 parts of a molecular sieve; 30 to 60 parts of a binder; 0.1-5 parts of an alkali metal auxiliary agent; the metal active component is one or more of Mo, Mn, Ce and Ni. The preparation method of the catalyst is simple and easy to implement, and the yield of ethylene and propylene prepared by catalytic cracking of Fischer-Tropsch synthesis light oil and crude oil is high.

The invention discloses a biosensor for capturing and detecting CTCs and a preparation method, and belongs to the technical field of biosensors. The invention solves the problem of how to further improve the sensitivity and specificity of capturing and detecting the circulating tumor cells. A composite nano material AuNPs-G / GDY is adopted as a substrate for modifying a sensing interface, nano gold ions can be combined and anchored with sulfydryl of tetrahedral DNA through Au-S bonds, a hanging chain of TDN takes cDNA as a template, a multivalent aptamer network layer is generated through RCA, and the multivalent aptamer network layer can capture low trace amount of CTCs. On the basis, the circulating tumor cells are specifically bound by using the aptamer of the circulating tumor cells on the PdPtCuRu-aptamer probe, and then the circulating tumor cells can be detected and quantified by catalyzing H2O2 reduction reaction to generate an electrochemical signal value. Therefore, the circulating tumor cells are clamped between the multivalent aptamer network layer and the PdPtCuRu-aptamer probe, and bidirectional sandwich capture of the cells and signal enhancement are realized.

The invention relates to a gelatin-based high internal phase emulsion as well as a preparation method and application thereof. The gelatin-based high internal phase emulsion comprises a water phase and an oil phase; the water phase is prepared from the following raw materials: gelatin, water, glutaraldehyde and a disulfide bond cross-linking agent; the preparation raw materials of the oil phase comprise liquid paraffin and a compound emulsifier. The gelatin-based high-internal-phase emulsion foam material prepared from the gelatin-based high-internal-phase emulsion provided by the invention has the advantages of high porosity, high pore structure connectivity, good structural stability and support performance, good biocompatibility, realization of digestion, and small influence of the digestion process and product on cell activity.

This invention discloses an intracellular imaging system for simultaneously detecting UDG and miR-375 expression levels and its applications. The intracellular imaging system uses a tetrahedral DNA nanostructure as a carrier, loading a HAT-UDG probe with an on / off conformation, and coupling it with a split CRISPR / Cas12a detection component for recognizing miR-375. The split CRISPR / Cas12a detection component includes at least Cas12a protein, scaffold RNA, spacer RNA / equivalent split crRNA components, and a fluorescent reporter substrate. This invention is the first to perform a logical AND operation between two key indicators: UDG enzyme activity and miR-375 expression level. The system can only ultimately trigger a complete fluorescence signal when both are simultaneously highly expressed. This multi-target synergistic activation design constructs a precise molecular recognition logic gate, enabling extremely accurate differentiation between prostate cancer cells (RV-1) and normal cells (293T), as well as other cancer cells (5637) that highly express UDG, fundamentally avoiding misjudgments caused by fluctuations in a single indicator or non-specific expression, resulting in extremely high imaging specificity.

The utility model relates to a product extraction device for producing acetone through synthesis gas pressure fermentation, which comprises a synthesis gas pressure fermentation tank, a membrane filtration unit, a reduced pressure flash tank, a gas condensation pipe and an acetone storage tank, a liquid inlet of the membrane filtration unit is communicated with a fermentation liquid discharge port, and a reflux inlet of the membrane filtration unit is communicated with the fermentation tank; a liquid inlet of the reduced-pressure flash evaporation pipe is communicated with a liquid outlet of the membrane filtration unit; a gas inlet of the gas condensation pipe is communicated with a vapor phase outlet of the vacuum flash tank; and a condensate outlet of the gas condensation pipe is communicated with an acetone storage tank. According to the product extraction device, firstly, thalli of fermentation liquor are intercepted through the membrane filtration unit, the activity of the thalli is reserved, and the influence on the activity of the thalli is low; according to the invention, acetone is used as a raw material, then the reduced pressure flash tank is used for reducing the pressure of the fermentation liquid and then flash evaporation is carried out, the temperature is maintained at 60 DEG C by using fermentation waste heat, so that the acetone is rapidly vaporized to form gas-liquid two-phase flow, and the design not only improves the vaporization efficiency of the acetone, but also reduces the additional cooling requirement and reduces the energy consumption.

The present invention relates to the technical field of stem cell collection, and in particular to a stem cell collection control system, which comprises an image acquisition module, a centrifuge, a magnetic bead sorting column, a flow cytometer and a control mechanism, wherein the control mechanism comprises a data acquisition module and a data analysis module for determining a stem cell collection method according to an activity evaluation value of the stem cells, wherein the data analysis module determines three collection methods according to the activity evaluation value: the first collection method is a density gradient centrifugation method, the second collection method is a magnetic bead sorting method, and the third collection method is a flow cytometric sorting method, and the data analysis module calculates the activity evaluation value according to the total number of stem cells and the number of positive stem cells in a test sample. The present invention overcomes the problems of poor accuracy in stem cell sorting and great influence on stem cell activity during the sorting process in the prior art.

The invention discloses a biological material droplet spraying device which comprises a fixed part and a non-fixed part convenient to disassemble, the fixed part comprises a shell, an upper limiting threaded column, an upper limiting piece, a piezoelectric ceramic stack, a lower limiting piece and a large spring, and an upper through hole and a lower through hole which are coaxial are formed in the top end and the bottom end of the shell respectively; the upper through hole is in threaded connection with part of the upper limiting threaded column, and the upper limiting piece, the piezoelectric ceramic pile, the lower limiting piece and the large spring are sequentially arranged in the shell from top to bottom; the non-fixed part comprises a circular ring piece, a cylindrical sleeve, a small spring, a cylindrical sleeve vertical guide and a liquid storage cylindrical needle, the lower through hole is in threaded connection with the part of the cylindrical sleeve vertical guide, and the circular ring piece and the small spring are arranged in the large spring. The device is small in size and convenient to disassemble and assemble, high-viscosity bio-ink can be printed, and the size and frequency of jet biological material liquid drops can be flexibly adjusted.

The present application provides a kind of oilfield sewage biological treatment process based on microbial technology, it is characterized in that, including the following steps: the oilfield sewage containing partial hydrolyzed polyacrylamide is sent into multiple alternately arranged acid-producing bacteria pool and acid-consuming bacteria pool in turn, sewage is alternately flowed between the acid-producing bacteria pool and the acid-consuming bacteria pool, the PH value of sewage is oscillated in above-mentioned range, and each oscillation period is 3-5 hours;The treated sewage enters aerobic biological treatment tank, and the aerobic biological treatment tank is added with compounded microbial inoculum, and the compounded microbial inoculum is composed of pseudomonas, bacillus subtilis and acinetobacter with mass ratio 3:2:1.The oilfield sewage biological treatment process based on microbial technology has the advantages that after physical method pretreatment, additional chemical treatment method is not needed, and the treatment effect of polyacrylamide-containing sewage can be greatly improved.

The invention discloses an astaxanthin mask as well as a preparation method and application thereof, and belongs to the technical field of skin care product processing. The astaxanthin mask is prepared from astaxanthin, a gynura succulent extract, an eclipta prostrata extract, an artemisia stelleriana extract, a trifolium repens extract, an artemisia scoparia extract, a chondrus crispus extract, an Okouguman tree resin extract, an aloe extract and conventional auxiliary materials during skin care product preparation. The components in the mask cooperate with each other and are easily absorbed by human skin. More possibilities are provided for practical application of astaxanthin, meanwhile, the astaxanthin mask has good whitening, moisturizing and anti-oxidation effects, the skin can keep good elasticity and compactness, skin discomfort can be effectively relieved, and the good acne removing function is achieved.

The invention provides a hematopoietic stem cell storage method. According to the technical scheme, a cell cryopreservation system with brand new components is firstly constructed, and a sectional type cryopreservation scheme is designed on the basis of the cell cryopreservation system. Specifically, the fetal calf serum is added to serve as a basic nutrient under the condition that dimethyl sulfoxide is reserved, meanwhile, sodium cromoglycate and chrysin are added to serve as protective components, and the freezing protection effect is achieved through the hydrophilicity of sodium polystyrenesulfonate and glycerol; in addition, the components such as the compound amino acid, the tea polyphenol and the arachidonic acid are added, so that the cell cryopreservation time is prolonged, and the cell activity is kept. In the cryopreservation process, the three-stage pre-cryopreservation process is firstly carried out under the conditions of low concentration and high temperature, and a protective agent is added, so that the damage of the cells in the pre-cryopreservation process is relieved; compared with direct cooling to-80 DEG C, the method has the advantages that the survival rate of the pre-cryopreserved cells is higher, the influence on the cell activity is smaller, and the differentiation capacity of the recovered cells is fully ensured.