7 results about "Cytotoxicity testing" patented technology

This invention discloses a method and system for cell viability detection using dual-channel differential dynamic adhesion impedance analysis. The system utilizes a microfluidic chip integrating detection and reference channels to synchronously apply fluid disturbances to both channels, inducing a dynamic process of "adhesion → detachment → re-adhesion" in cells. During this process, the differential impedance curve (after background subtraction) is acquired in real time, and six dynamic feature parameters, including the amplitude and rate of decrease and recovery, are extracted. A ridge regression algorithm is used to construct a cell viability prediction model, and the six dynamic feature parameters are input into the model to obtain cell viability values. This invention effectively eliminates environmental and fluid common-mode noise through differential structure and achieves label-free, in-situ, highly sensitive measurement of cell viability using impedance analysis. It has advantages such as accurate results, good stability, and high automation, and is suitable for fields such as cytotoxicity testing and drug screening.

The invention discloses application of neochlorogenic acid in preparation of a medicine for preventing and / or treating hand-foot-and-mouth disease caused by A16 type coxsackie virus (CVA16) infection, and belongs to the technical field of biological medicine. Researches prove that the neochlorogenic acid has the effect of resisting CVA16 infection on HEK293T cells and H4 cells, after the two cells treated by the neochlorogenic acid are infected by the CVA16, the viral genome RNA abundance is obviously reduced, and the cell lesion death condition is obviously relieved; meanwhile, a cytotoxicity test shows that the neochlorogenic acid has no obvious cytotoxicity to HEK293T cells and only has weak cytotoxicity to H4 cells. Therefore, in a reasonable dosage range, the neochlorogenic acid, which is a low-toxicity natural small molecule compound, can be used for preparing the anti-CVA16 medicine for preventing and / or treating the hand-foot-and-mouth disease caused by CVA16 infection.

The invention provides low-toxicity and low-sensitization fluororubber watchband glue as well as a preparation method and application thereof, and belongs to the technical field of rubber materials. According to the technical scheme of the invention, the raw fluororubber is polymerized by adopting the surfactant containing no fluorine element, and meanwhile, the reinforcing filler system is optimized, so that the skin sensitization and stimulation risks are remarkably reduced. The fluororubber watchband adhesive prepared by the preparation method disclosed by the invention shows excellent biological safety in a cytotoxicity test. The invention provides a novel environment-friendly material solution with safety and reliability for the field of wearable equipment, particularly watchband products in long-term contact with human skin, and has definite market application prospect and popularization value.

This invention provides naphthoquinone compounds targeting the novel coronavirus and their pharmaceutical uses. In vitro activity assays show that the compounds disclosed in this invention can inhibit the main protease (Mpro) activity of the SARS-CoV-2 novel coronavirus. In vitro activity assays show that some compounds, at a concentration of 1 μM, exhibit an enzyme inhibition rate of over 90%, significantly superior to the positive control drug shikonin, and can effectively reduce the replication capacity of wild-type and various mutant strains of the novel coronavirus in the host. In vitro cytotoxicity assays show that the compounds disclosed in this patent have significantly lower toxicity to normal host cells (HSF cells) than the positive control drug shikonin, indicating promising application prospects as drug candidates against COVID-19 infection.

The invention provides an acylhydrazone-Mn complex as well as a preparation method and application thereof. The structural formula of the acylhydrazone-Mn complex is shown in the specification, or in the structural formula, R is a substituent group. According to the invention, 4-diphenylaminobenzoylhydrazone reacts with MnCl2, the acylhydrazone-Mn complex is obtained by utilizing easy formation, strong coordination ability, diverse applications and biological activity of acylhydrazone compounds and utilizing N, O atoms and a plurality of metal ions to form complexes with different structures and properties, and some complexes have the characteristics of anti-tumor and antibacterial activity. A cytotoxicity test, an in-vitro proliferation inhibition activity experiment and an antibacterial experiment show that the synthesized Mn complex not only has a relatively good inhibition effect on tumor cells, but also has relatively low toxicity on normal cells; in addition, the proliferation of P.micro can be effectively inhibited.

The application discloses a lysosome-targeting near-infrared fluorescent probe and a preparation method and application thereof in carboxylesterase detection, wherein the structure of the lysosome-targeting near-infrared fluorescent probe is shown in the following formula: The lysosome-targeting near-infrared fluorescent probe of the application shows a rapid response (response time is 40 seconds) to carboxylesterase (CE) in an in-vitro experiment. Cell toxicity test shows that the probe has low biological toxicity, and confocal fluorescence microscopic imaging experiment shows that the fluorescent probe has good light stability in HeLa cells and can effectively target lysosomes (localization coefficient is 0.86), and is suitable for CE near-infrared region fluorescence imaging and detection in lysosomes of living cells.

The application discloses a kind of detection tyrosinase dual-channel near-infrared ratio fluorescent probe and its preparation method and purposes, wherein the structure of detection tyrosinase dual-channel near-infrared ratio fluorescent probe is as follows: the dual-channel near-infrared ratio fluorescent probe LZD of the application shows good selectivity and sensitive dual-channel fluorescence signal to tyrosinase.Cytotoxicity test shows that the ratio probe has good biocompatibility, and confocal fluorescence microscopic imaging experiment shows that the ratio probe has good light stability to HepG2 cells, can respond to tyrosinase through dual-channel fluorescence, and can carry out confocal fluorescence imaging to extracellular and endogenous tyrosinase of cell.