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Preparation method for TIA yeast peptide fermentation product filtrate and function application thereof in skin-whitening and freckle-removing

A fermentation product and skin whitening technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as product safety problems, unfavorable human body care use, and many chemical impurities, and achieve cell damage repair capabilities. Strong, stable, and tyrosinase inhibitory

Inactive Publication Date: 2018-05-25
重庆雅素生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its process is cumbersome and introduces many chemical impurities, which is easy to cause damage to active ingredients and has problems with product safety, which is not conducive to human body care.

Method used

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  • Preparation method for TIA yeast peptide fermentation product filtrate and function application thereof in skin-whitening and freckle-removing
  • Preparation method for TIA yeast peptide fermentation product filtrate and function application thereof in skin-whitening and freckle-removing
  • Preparation method for TIA yeast peptide fermentation product filtrate and function application thereof in skin-whitening and freckle-removing

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preparation example Construction

[0028] The present invention provides a technical solution: a preparation method of TIA yeast peptide fermentation product filtrate and its functional application in skin whitening and freckle removal, comprising the following steps:

[0029] S1. Select the rice, wash it 1-2 times with deionized water, soak it at room temperature for 6-12 hours, and control the room temperature at 20-25°C.

[0030] S2. Crush the soaked rice with a grinder, add distilled water to stir and homogenize, saccharify for 2-12 hours, and cool to 25-30°C.

[0031] S3. Use the Pseudomonas yeast strains screened out by amplification to make the density of the bacteria reach 0.5OD-2.5OD, and add them for use after fermentation.

[0032] S4. Add the saccharified rice homogenate to the medium, then add 1.0-10% vegetable oil rich in oleic acid to the medium, steam sterilize for 30-45 minutes, cool to 25-30° C. for inoculation.

[0033] S5. Put the inoculated culture medium into a thermostatic chamber, and f...

Embodiment 1

[0063] A method for preparing TIA yeast peptide fermentation product filtrate and its functional application in skin whitening and freckle removal, comprising the following steps:

[0064] S1. Select the rice, wash it once with deionized water, soak it at room temperature for 6 hours, and control the room temperature at 20°C.

[0065] S2. Crush the soaked rice with a grinder, add distilled water, stir and homogenize, saccharify for 2 hours, and cool to 25°C.

[0066] S3. Use the Pseudomonas yeast strains screened out by amplification to make the density of the strains reach 0.50D, and add them for use after fermentation.

[0067] S4. Add the saccharified rice homogenate to the medium, then add 1.0% vegetable oil rich in oleic acid to the medium, steam sterilize for 30 minutes, and cool to 25° C. for inoculation.

[0068] S5. Put the inoculated culture medium into a thermostatic chamber, and ferment for 48 hours with controlled temperature and oxygen feeding, and the temperatu...

Embodiment 2

[0074] A method for preparing TIA yeast peptide fermentation product filtrate and its functional application in skin whitening and freckle removal, comprising the following steps:

[0075] S1. Select the rice, wash it twice with deionized water, soak it at room temperature for 9 hours, and control the room temperature at 23°C.

[0076] S2. Grinding the soaked rice with a grinder, adding distilled water, stirring and homogenizing, saccharifying for 2-12 hours, and cooling to 28°C.

[0077] S3. Use the Pseudomonas yeast strains screened out by amplification to make the density of the strains reach 1.0 OD, and add them for use after fermentation.

[0078] S4. Add the saccharified rice homogenate to the medium, then add 5% vegetable oil rich in oleic acid to the medium, steam sterilize for 40 minutes, and cool to 28° C. for inoculation.

[0079] S5. Put the inoculated culture medium into a thermostatic chamber, and ferment for 72 hours with controlled temperature and oxygen feedi...

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Abstract

The invention discloses a preparation method for TIA yeast peptide fermentation product filtrate and a function application thereof in skin-whitening and freckle-removing. The preparation method comprises the following steps: S1, carefully selecting rice, washing for 1-2 times by using deionized water, and soaking for 6-12 hours in the room temperature; S2, crushing the soaked rice by using a crushing machine, adding distilled water and stirring until the homogenate, saccharifying for 2-12 hours, and cooling to 25-30 DEG C; S3, using pseudomonas saccharomycetes screened out by the amplification, enabling the bacteria density to be 0.5-2.5 OD, for the future fermentation adding and using; and S4, adding the saccharified rice homogenate to a culture medium. The preparation method is capableof performing the saccharomycetes fermentation by using the food material rice and vegetable edible fat and oil. The obtained wall-broken filtrate is safe completely without any external stimulation,and a cytotoxicity test shows the zero. The raw material obtained by using the preparation method can be used for preparing skin-whitening freckle-removing drugs or cosmetics, the cytotoxicity is zero, the raw material is safe and effective without the stimulation, good in stability, and is capable of avoiding the defects that a chemical whitening agent and a plant extractant can injure and stimulate a human body while used for whitening the skin, and the stability is bad.

Description

technical field [0001] The invention relates to the technical field of preparation method of TIA yeast peptide fermentation product filtrate and its functional application in skin whitening and freckle removal, specifically a preparation method of TIA yeast peptide fermentation product filtrate and its functional application in skin whitening and freckle removal. Background technique [0002] TIA Yeast Peptide uses an original microbial fermentation process to prepare active peptides, and directly uses the hydrolyzed protein to cultivate protease-producing bacteria, and uses the protease secreted by the bacteria to directly dissolve the yeast. It is separated and purified by high-tech biological shearing and broken cells. It is a natural, organic, efficient, healthy and safe bionic whitening skin care active ingredient to protect the skin from external stress damage, and the skin derives natural and healthy skin. White. [0003] Tyrosinase is a copper-containing enzyme deri...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/9794A61Q19/02A61Q19/08
CPCA61K8/64A61K8/97A61K2800/85A61Q19/02A61Q19/08
Inventor 周朝强陈洋
Owner 重庆雅素生物科技有限公司
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