Direct exposure in-vitro toxicity test method for electronic cigarette aerosol

Abstract

The invention provides a direct exposure in-vitro toxicity test method for electronic cigarette aerosol. The method is characterized in that testing of various cytotoxic terminal points and detectionof release levels of various inflammatory factors can be carried out, and the in-vitro toxicity effect of the electronic cigarette aerosol is reflected more comprehensively. The method specifically comprises the following steps of generation of the electronic cigarette aerosol, direct exposure of cells, testing of cytoxicity and detection of the inflammatory factors. According to the method, an in-vitro toxicity determination method of the electronic cigarette aerosol is established, and an in-vitro cell direct exposure experiment of the electronic cigarette aerosol, testing of the cytotoxic terminal points and the detection of the release levels of the inflammatory factors are integrated. After one-time exposure, the testing of the cytotoxic terminal points can be carried out on the cells, the detection of the inflammatory factors can be carried out on a cell supernatant, the evaluated toxic terminal points are numerous and efficient, the flux is high, and the in-vitro toxicity effectof the electronic cigarette aerosol can be represented comprehensively from multiple aspects.

Description

technical field [0001] The invention relates to the research field of in vitro toxicological evaluation of electronic cigarettes, in particular to an in vitro toxicity test method for direct exposure of electronic cigarette aerosol. Background technique [0002] Electronic cigarette, also known as electronic nicotine delivery system, is a product that atomizes electronic cigarette liquid through an atomizer to deliver nicotine and / or other substances to the respiratory system. Compared with traditional cigarettes, e-cigarettes have a different way of generating smoke aerosols, and there is no combustion and cracking process. The emergence and use of e-cigarettes has also aroused public health concerns. However, there are very few preclinical toxicological evaluation studies on e-cigarettes. The existing in vitro toxicological evaluation of e-cigarettes is mainly based on toxicological testing experiments on e-cigarette liquid, and there is no toxicity evaluation on the aero...

AI Technical Summary

Problems solved by technology

The evaluation of freshly generated e-cigarette aerosols can better reflect the biological effects of e-cigarette smoke in the actual exposure environment, while the previous method of poisoning with e-cigarette liquid solution cannot evaluate the toxicology of actually inhaled e-cigarette aerosol components effect

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