Method and kit for simultaneously determining contents of three free androgens in serum

An androgen and kit technology is applied in the field of simultaneous determination of three free androgens in serum, which can solve the problems of lack of high-quality free hormone determination methods, insufficient accuracy, and good reproducibility, and achieve rapid enrichment and Purification, accuracy and precision, and reproducible results

Pending Publication Date: 2022-07-01
HEFEI NOVA-MS BIOTECH MEDICAL EQUIP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For the detection of ASD, DHEA and DHEAS in serum by the existing high performance liquid chromatography tandem mass spectrometry technology, the immunoassay method can only measure a single substance, and the determination of multiple components requires a large amount of sample volume and time, and at the same time The determination of multi-component androgen lacks high-quality free-state hormone determination methods, and indirect tests are mostly used for the determination of free-state hormones. The basic method is to measure total hormones and total protein, and then use the mass equation to theoretically estimate free-state hormones. Due to the technical problems of insufficient accuracy and cumbersome operation, this application provides a method for simultaneously measuring the contents of three free androgens in serum, which has the advantages of low detection limit, high accuracy, good reproducibility, and short time-consuming

Method used

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  • Method and kit for simultaneously determining contents of three free androgens in serum
  • Method and kit for simultaneously determining contents of three free androgens in serum
  • Method and kit for simultaneously determining contents of three free androgens in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A kit for simultaneous determination of the content of three free androgens in serum of the present embodiment, the kit includes the following components:

[0054] Table 1 Components of the kit for detecting free androgen content in serum

[0055]

[0056] In this example, before the detection, the calibration curve solution was diluted with a surrogate matrix, and the concentration gradient of ASD was 0.02, 0.05, 0.2, 0.5, 1, 2, 5, and 10 ng / mL; the concentration gradient of DHEA was 1 , 2.5, 10, 25, 50, 100, 250, 500ng / mL; the concentration gradient of DHEAS was 10, 25, 100, 250, 500, 1000, 2500, 5000ng / mL. The quality control solution was diluted with surrogate matrix, and its concentrations were ASD: 0.1, 1, 5 ng / mL; DHEA: 5, 50, 250 ng / mL; DHEAS: 50, 500, 2500 ng / mL. The concentrations of internal standards ASD-d7, DHEA-d6 and DHEAS-d6 were 1 ng / mL, 50 ng / mL and 500 ng / mL, respectively. In the kit of this example, the collected serum samples do not need deriva...

Embodiment 2

[0058] A kit for simultaneous determination of the content of three free androgens in serum of this embodiment is basically the same as that of Embodiment 1, the difference is the concentrations and gradients of the calibrator solution, the quality control solution and the internal standard solution.

[0059] Table 2 Components of the kit for detecting free androgen content in serum

[0060]

[0061] In the kit of this example, the concentration gradient of ASD in the calibrator solution is 0.02, 0.05, 0.2, 0.5, 1, 2, 5, 10 ng / mL; the concentration gradient of DHEA is 1, 2.5, 10, 25, 50 , 100, 250, 500ng / mL; the concentration gradient of DHEAS is 10, 25, 100, 250, 500, 1000, 2500, 5000ng / mL. The concentrations of the quality control solutions were ASD: 0.1, 1, 5 ng / mL; DHEA: 5, 50, 250 ng / mL; DHEAS: 50, 500, 2500 ng / mL. There is no need to dilute before the test, the operation is simpler, and the test time is saved. It is suitable for use scenarios such as large hospitals ...

Embodiment 3

[0063] A kit for simultaneous determination of the content of three free androgens in serum of this embodiment is basically the same as that of embodiment 2, except that the types of extractants are different.

[0064] Table 3 Components of the kit for detecting free androgen content in serum

[0065]

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Abstract

The invention belongs to the technical field of hormone detection, and relates to a method and a kit for simultaneously determining the content of three free androgens in serum. The method aims at solving the problems that when ADS, DHEA and DHEAS in serum are detected through an existing high performance liquid chromatography-tandem mass spectrometry technology, a large amount of sample amount and time are needed for multi-component determination, a high-quality free-state hormone determination means is lacked, indirect testing is mostly used for free-state hormone determination, the basic means is total hormone and protein total amount measurement, and the detection time is short. In order to solve the technical problems of insufficient accuracy, troublesome operation and the like in the prior art, the invention provides the method for simultaneously detecting the content of the three free androgens in the serum, and the method has the advantages of low detection limit, high accuracy, good reproducibility and short time consumption. The invention further provides a kit for simultaneously determining the content of the three free androgens in the serum, detection can be achieved only through 300 microliters of serum samples, and operation is rapid, simple and convenient.

Description

technical field [0001] The invention belongs to the technical field of hormone detection, and in particular relates to a method and a kit for simultaneously measuring the contents of three free androgens in serum. Background technique [0002] Quantitative determination of steroid hormones is important for assessing adrenal and gonadal function, differential diagnosis, and monitoring of therapeutic drugs, both in adults, adolescents, and neonates, and is particularly important in the diagnosis of neonatal congenital adrenal hyperplasia and steroid synthesis defects. Measurements of androgens in many laboratories are often limited to testosterone. For a more accurate assessment of androgen status in both sexes, measuring other androgens, such as androstenedione (ASD), dehydroepiandrosterone (DHEA), and dehydroepiandrosterone sulfate (DHEAS), can provide a more complete picture of women Information on the most abundant circulating androgens in the body. [0003] ASD and DHEA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/72
CPCG01N30/06G01N30/72G01N2030/062
Inventor 彭军江振作刘洋谢沙莎
Owner HEFEI NOVA-MS BIOTECH MEDICAL EQUIP CO LTD
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