117 results about "Protein total" patented technology

The present invention relates to a whey protein product and a preparation method therefor. The product is derived from a whey protein-based protein composition, and the protein composition comprises at least a portion of whey protein degradation components, wherein protein components in the protein composition have a molecular weight distribution of: (i) the content of the protein component having a molecular weight of less than 5000 Da is 80-96% by mass; (ii) the content of the protein component having a molecular weight of 5000 Da to 10,000 Da is 0.5-10% by mass; and (iii) the content of protein component having a molecular weight of greater than 10,000 Da is 0-10% by mass, the content being the content of each of the three protein components in the total protein components based on dry weight.

The present invention provides an infant formula milk powder and a preparation method thereof. The infant formula milk powder contains a protein composition capable of improving the digestibility of protein in the infant formula milk powder; wherein, based on the total protein content of the infant formula milk powder as 100%, the protein composition contains 13.0%-50.5% of α-lactalbumin and 15.0%-35.0% of β-casein. The technical solution of the present invention can significantly improve the digestibility of whey protein in infant formula milk powder.

The invention discloses a composite microecological preparation as well as a preparation method and application thereof. The compound microecological preparation disclosed by the invention comprises lactobacillus casei, lactobacillus plantarum and saccharomyces cerevisiae. The application effect of the composite microecological preparation in cow breeding is remarkable, the optimal addition amount of the composite microecological preparation is 100 mL / cow / day, the production performance of lactating cows can be improved, the milk yield is improved by 3.30 kg / cow / day, the milk yield of 4% standard milk is improved by 3.78 kg / cow / day, the total protein is improved by 15.9%, the albumin is improved by 14.7%, the globulin is improved by 16.5%, and the somatic cell number is reduced by 52.6%; and pollutant emission can be reduced, including 37.2% of ammonia emission reduction and 28.7% of carbon dioxide emission reduction.

The invention belongs to the technical field of protein content determination, and particularly relates to a method for determining the protein content of corn kernels. The method comprises the following steps: extraction of corn kernel proteins: extracting different types of corn kernel proteins respectively; determining the protein content of the corn kernels: drawing a standard curve by using a bovine serum standard protein solution; calculating to obtain different types of corn kernel proteins; the sum of the contents of the proteins of the different types of corn kernels is the total protein content of the corn kernel sample. According to the method, the protein components in the corn kernel sample are extracted by adopting a sequential extraction method, each protein component can be completely extracted, the use amount of the sample is small, the operation process is simple, the cost is relatively low, and a basis is provided for accurately measuring the content of the protein components in the corn kernels. According to the method, toxic reagents such as concentrated sulfuric acid, lauryl sodium sulfate, phenolic substances and mercaptoethanol are not used, so that the health of operators is not damaged, and the safety in the experiment process is ensured.

A lyophilized composition including polyclonal immunoglobulin M (IgM), including the steps of: a) providing an initial aqueous solution including IgM at a concentration between about 10 mg / mL and about 50 mg / mL, the polyclonal IgM being at least about 90% by weight of the total protein content of the composition; b) adding amino acids selected from the group consisting of proline, glycine, alanine, valine and hydroxyproline or a mixture thereof at a final concentration of about 0.15 M to about 0.45 M; polysorbate 80 (PS80) at a concentration between about 50 and about 200 ppm; and succinic acid at a concentration between about 1 mM and about 20 mM; and c) lyophilizing the IgM composition obtained in step b); wherein the lyophilized composition has a content of pentameric IgM higher than about 90% of the total IgM content, a content of IgM aggregates of less than about 1.5%.

The invention discloses a protein extracting solution and a protein extracting method of polysaccharide and polyphenol plant tissues, and is characterized in that the protein extracting solution comprises the following components in molar ratio: 180-250 parts of trihydroxymethyl aminomethane hydrochloride buffer solution, 200-300 parts of sugar, 45-55 parts of chlorate, 10-70 parts of beta-mercaptoethanol and 0.5-2 parts of protease inhibitor. The browning condition of the total protein solution obtained by the protein extraction formula is obviously weakened, which indicates that the oxidation of polyphenol is obviously inhibited, the release of protein in a tissue sample is facilitated, and the extracted protein effect is better.

The invention belongs to the technical field of protein interaction prediction, and discloses a protein interaction prediction method and system based on dynamic graph attention, and the method comprises the following steps: 1, constructing a protein total graph; step 2, graph node feature enhancement: adjusting feature representation of residue nodes in the protein graph by using a gating mechanism, GRU time sequence updating and an additive attention mechanism, and performing adaptive enhancement on residue node features; step 3, predicting protein-protein interaction: aggregating information from adjacent nodes by adopting a dynamic graph attention mechanism, and capturing local and global interaction between residues; and splicing the output features of the two protein maps, and generating a score for predicting protein-protein interaction after passing through a full connection layer. According to the method, more accurate and stable protein interaction prediction is realized through dynamic feature fusion of a double-protein map structure.

The invention discloses application of bacillus subtilis QST713 in improving the production performance and reproduction traits of Xiaoshan chickens. The production performance of the Xiaoshan chickens is improved by any one or more than two of the following steps: (1) reducing the feed-egg ratio of the Xiaoshan chickens; (2) the egg weight of Xiaoshan chicken is increased; (3) the egg protein height of Xiaoshan chicken is increased; (4) increasing any one or more of total protein, globulin and albumin in serum of the Xiaoshan chicken; (5) reducing any one or more of cholesterol, triglyceride, glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and low-density lipoprotein in serum of the Xiaoshan chicken; and (6) increasing any one or more of immune globulin A, immune globulin G and immune globulin M in the serum of the Xiaoshan chicken. By adding the bacillus subtilis QST713 strain, the feed-egg ratio of the Xiaoshan chicken can be remarkably reduced, the egg quality and serum biochemical indexes of the Xiaoshan chicken can be effectively improved, the immune function is enhanced, and the ovarian gene expression of the Xiaoshan chicken is influenced.

The invention discloses a transcription factor GPC6 for regulating and controlling the protein content of rice grains and application of the transcription factor GPC6. The amino acid sequence of the transcription factor is shown as SEQ ID NO.1. The transcription factor comprises a genome region and a promoter, the nucleotide sequence of the genome region is shown as SEQ ID NO.2, the nucleotide sequence of the promoter is shown as SEQ ID NO.3, the coding sequence (CDS) is shown as SEQ ID NO.4, and the coding sequence (CDS) is shown as SEQ ID NO.3. The transcription factor GPC6 can be used for regulating and controlling the protein content of the rice grains. And comprises all alleles thereof. Whole genome association analysis is carried out on the glutelin content and the total protein content, and a site GPC6 capable of being repeatedly detected is identified on a No.6 chromosome. Gene cloning is carried out, gene resources for rice quality genetic improvement can be enriched, and help is provided for rice quality improvement, especially protein content improvement.

The application discloses a transcription factor for regulating protein content of rice grains GPC6 and application, the amino acid sequence of the transcription factor is shown as SEQ ID NO. 1, the transcription factor comprises a genomic region and a promoter, the nucleotide sequence of the genomic region is shown as SEQ ID NO. 2, the nucleotide sequence of the promoter is shown as SEQ ID NO. 3, the coding sequence (CDS) is shown as SEQ ID NO. 4, and all alleles thereof are contained. The application identifies a repeatedly detectable locus on chromosome 6 by performing whole genome association analysis on gluten content and total protein content GPC6 . Gene cloning is carried out on the locus, so that the genetic resources for improving rice quality are enriched, and help is provided for improving rice quality, in particular, protein content.

To provide fermented milk which is high in protein but has low hardness and does not harden during storage, and a manufacturing method of the same.SOLUTION: The above-mentioned problems can be solved by making a protein content of fermented milk 8.0 wt.% or more based on the total weight, and making the weight ratio of the protein derived from a microparticulated whey protein material to the total protein 0.50 or more. The fermented milk of the present invention has a hardness of less than 50 gf. The microparticulated whey protein material used in the fermented milk of the present invention has (a) a weight ratio of the protein to the total solid of less than 0.60, (b) a 50% particle size (D50) of 0.5 to 10 μm, and (c) a modification degree of the whey protein of 60% or more.SELECTED DRAWING: Figure 1

The invention discloses a corn ZmGLN3 gene and application of the corn ZmGLN3 gene in increasing the protein content of plant stems. QTL positioning is carried out based on a BC2S2 group of wild corn Ames21814 and modern cultivated corn B73, a key gene ZmGLN3 for controlling the corn stalk protein content is excavated, and structural variation analysis finds that the ZmGLN3 gene has tandem repeat variation in the Ames21814, so that the expression quantity of the gene in the Ames21814 is remarkably improved, and the total protein content of the stalk and the whole plant of the Ames21814 is further effectively improved. The invention also finds that the stem protein content of the ZmGLN3 gene overexpression material is also obviously improved. The discovery of the ZmGLN3 gene and the excellent haplotype thereof provides an efficient means for genetic improvement and germplasm resource innovation of whole-plant silage corn, and has important application value and economic prospect.

The invention relates to a process for dephosphorylation of skim milk, ultra-filtered milk (UF milk) or micellar casein isolate (MCI) comprising (i) acidification of a skim milk, UF milk or MCI preferably not lower than pH 6.0, preferably between 6.0 and 6.7, (ii) cooling the acidified skim milk, UF milk or MCI to the temperature between 0° C. and 15° C., (iii) adding gluconate and / or maleate to the cooled skim milk, UF milk or MCI and, (iv) washing the skim milk, UF milk or MCI, to remove phosphorus, thus dephosphorylating the skim milk, UF milk or MCI, preferably to an extent that the total phosphorous content of the skim milk, UF milk or MCI is reduced with at least 20%, preferably 20-40%, more preferably 30-40% compared to the material provided to step (i). The invention also relates to dephosphorylated MPC, MPI or MCI obtainable by the process of the invention, and to a liquid heat-sterilized enteral nutritional composition comprising 2.0-3.0 kcal / ml wherein 16-35 en % is provided by protein, the combination of caloric content and relative protein caloric content selected such that there is 10-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein in the composition, wherein the protein comprises micellar casein (MC), whey protein (WP) and optionally caseinate (CAS), wherein there is at least 70 wt % MC and less than 15 wt % WP, based on total protein content, and wherein the composition has a total amount of phosphorous less than 192 mg / 100 ml and / or 30-80 mg / 100 kcal, preferably at least 30-80 mg / 100 kcal, most preferably less than 192 mg / 100 ml and 30-80 mg / 100 kcal.

The invention relates to the field of nutritional compositions, methods for providing them and uses thereof. More in particular, it relates to ready-to-feed and powdered infant or growing up milk formulas showing desirable gastric digestion properties. Provided is a nutritional composition comprising casein, whey proteins comprising α-lactalbumin (aLAc) and β-lactoglobulin (bLac), and protein-coated fat droplets, wherein (i) the protein content of the composition is 5 to 20% (w / w), preferably 7-16 w %, based on total solids; (ii) the aLac+bLac content is at least 25% (w / w) based on the total protein level; (iii) the fat content is in the range of 10% to 50% (w / w) based on total solids; and (iv) the fat droplets are coated with an average protein load of 2-10 mg / m2 and wherein the weight ratio of casein:(aLac+bLac) on the fat droplets is at least 4 times higher, preferably at least 4.5 times higher, than the weight ratio of casein:whey protein in the total composition.

The present invention relates to a method for producing a lactoferrin-containing product by performing cation exchange with milk having a lactoferrin concentration of at least 4.0 per gram of protein. The invention also relates to a lactoferrin-containing product containing at least 96 wt% of lactoferrin and less than 0.60 wt% of immunoglobulin (IgG + IgA + IgM), based on the total protein.

Corn-unconventional protein source growing pig diet and feeding method thereof, relating to an improved unconventional protein source feed and feeding method thereof, belonging to the technical field of growing pig feed preparation. The invention is to solve the problem of soybean meal shortage. The corn-unconventional protein source growing pig diet is as follows: it consists of 55.0-65.0 parts of corn, 1.0-5.0 parts of wheat bran, 15.0-20.0 parts of soybean meal, 1.5-3.0 parts of rapeseed meal, 1.5-3.0 parts of cottonseed meal, 6.0-10.0 parts of distiller's grains protein feed (DDGS), 1.0-3.0 parts of sucrose, 1.0-3.0 parts of soybean oil, 0.1-0.5 parts of lysine hydrochloride, 0.01-0.05 parts of methionine, 0.05-0.1 parts of cysteine, 0.05-0.1 parts of threonine, 1.0-1.5 parts of stone powder, 1.0-1.5 parts of calcium hydrogen phosphate, 0.5-1.0 parts of sodium chloride and 1.0 parts of premix; the feeding method of the corn-unconventional protein source growing pig diet is feeding 6 times a day. Unconventional protein sources account for 20.0%-25.0% of total protein, reducing soybean meal by 5.0%-8.0%.

Disclosed in the present invention are a ketamine small molecule probe, and a preparation method therefor and the use thereof. Two small molecular probes are designed and synthesized, and the blank in the prior art is filled. A target fishing experiment is performed on the total protein in a cell lysis buffer by means of using the two probes, and 61 and 70 target proteins are obtained, respectively. In addition, more than three technical repetitions verify that the two probes can stably bind to the target proteins. A screened SIRT2 protein is used as a target protein of ketamine, and a reference basis is provided for the synthesis and preparation of an anti-inflammatory related drug.

To provide a nutritive composition for infants having an amino acid composition more similar to breast milk, making it possible to ensure sufficient amino acid nutrition without excessive ingestion of protein.SOLUTION: The present disclosure provides a nutritive composition for infants containing protein. In the composition, the total content of phenylalanine and tyrosine is 7.6 wt.% or more in terms of total amino acid content, and 90 wt.% or more of the total protein content is derived from milk.SELECTED DRAWING: Figure 1

The present invention relates to a process for the dephosphorylation of skimmed milk, ultra-filtered milk (UF milk) or micellar casein isolate (MCI) comprising (i) acidifying the skimmed milk, UF milk or MCI, preferably not below pH 6.0, preferably between 6.0 and 6.7, (ii) cooling the acidified skimmed milk, UF milk or MCI to a temperature between 0 DEG C and 15 DEG C, (iii) adding gluconate and / or maleate to the cooled skimmed milk, UF milk or MCI, and (iv) washing the skimmed milk, UF milk or MCI to remove phosphorus, thereby dephosphorylating the skimmed milk, UF milk or MCI, preferably to the extent that the total phosphorus content of the skimmed milk, UF milk or MCI is reduced by at least 20%, preferably 20-40%, more preferably 30-40%, compared to the material provided to step (i). The invention also relates to a dephosphorylated MPC, MPI or MCI obtainable by the process of the invention and to a liquid enteral nutritional composition comprising 2.0-3.0 kcal / ml of heat sterilization wherein 16 en%-35 en% is provided by a protein, the combination of the calorie content and the relative protein calorie content being selected such that 10-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein is present in the composition, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein. Wherein the protein comprises micellar casein (MC), whey protein (WP) and optionally caseinate (CAS) wherein at least 70 wt% MC and less than 15 wt% WP are present, based on the total protein content, and wherein the composition has a total amount of phosphorus of less than 192 mg / 100 ml and / or 30-80 mg / 100 kcal, preferably at least 30-80 mg / 100 kcal, most preferably less than 192 mg / 100 ml and 30-80 mg / 100 kcal.

The present invention relates to the field of nutritional compositions, methods of providing nutritional compositions and uses thereof. More particularly, the present invention relates to a ready-to-eat powdered infant formula or ready-to-eat powdered growth formula exhibiting desirable gastric digestion characteristics. There is provided a nutritional composition comprising casein, whey protein comprising alpha-lactalbumin (aLac) and beta-lactoglobulin (bLac), and protein-coated fat droplets wherein the composition has a protein content of from 5% to 20% (w / w) based on total solids; the bLac content is at least 3% (w / w) based on the total protein level, and the aLac + bLac content is at least 25% (w / w) based on the total protein level; the fat content is in the range of 10% to 50% (w / w) based on total solids; the fat droplets are coated with an average protein load of 2 to 10 mg / m2, and wherein the weight ratio of casein: (aLacc + bLac) on the fat droplets is at least 4 times the weight ratio of casein: (aLacc + bLac) in the total composition; the level of sphingomyelin per 100 g of the composition is between 70 mg and 200 mg; the level of phospholipids per 100 g of the composition is between 280 mg and 950 mg.

To provide a beer taste non-alcohol beverage that is excellent in flavor (for example, taste thickness, bitterness, and harshness) and in foam keeping.SOLUTION: A beer taste beverage has an alcohol content of less than 1 v / v%, a total polyphenol amount of 100 mg / L or more, a total protein amount of more than 0 μg / mL and 100 μg / mL or less, and an NIBEM value of 250 s or more.SELECTED DRAWING: None

Provided is a homogeneous composition comprising protein, wherein at least 1 wt % of a total protein content is bacterial protein: and a carrier selected from the group consisting of water, oil and a combination thereof, wherein a total protein content in the composition is at least 25 wt % or wherein the composition comprises at least one animal protein and a total protein content in the composition is at least 5 wt % of the total composition. Further provided are food ingredients and food products comprising the composition.

[Problem] There is a demand for a protein-rich food having excellent protein quality and quantity and a texture required in the market, and having excellent flavor. [Solution] The problem is solved by providing a protein-rich food having a total protein content of 10 g or more per 100 g and contains (A) a soybean protein and (B) a micellar casein protein, wherein the content of the soybean protein to the total protein mass is 2-70 mass%.

The present invention proposes a dry chemical reagent sheet for quantitatively detecting total protein and a preparation method thereof, and relates to the field of chemical reagent sheets. The dry chemical reagent sheet for quantitatively detecting total protein comprises a support layer, a reagent layer and a diffusion layer, wherein the reagent layer is arranged above the support layer, the diffusion layer is arranged above the reagent layer, the diffusion layer comprises a diffusion layer reagent or is formed by a diffusion layer reagent, the diffusion layer reagent comprises an adhesive, a surfactant and particulate matter, and the particulate matter comprises a first particulate matter and a second particulate matter; the number ratio of the first particulate matter to the second particulate matter is 1:(0.25-4), and the difference in particle size between the first particulate matter and the second particulate matter is 10-40 μm. The dry sheet for quantitative detection of total protein provided by the present invention is used for in vitro diagnosis, and its detection result is accurate, storage is stable, and it has the advantages of rapid diagnosis, simple operation, cost saving, etc.

The invention relates to a swimming bladder hydrolysate capable of improving a male reproductive function as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention provides a swimming bladder hydrolysate capable of improving a male reproductive function. The swimming bladder hydrolysate is obtained by performing enzymolysis on sturgeon swimming bladders through trypsin. The total protein content of the swimming bladder hydrolysate is 80%-90%; the amino acid composition of the swimming bladder hydrolysate comprises glycine, proline, glutamic acid, asparaginic acid, arginine, alanine, serine, lysine, threonine, phenylalanine, leucine, isoleucine, valine, histidine, tyrosine and methionine. Researches show that the swim bladder hydrolysate has very strong antioxidant activity, and the swim bladder hydrolysate can effectively relieve matt hair, unstable spirit, reduced exploration behavior, weight loss and male reproductive function damage of model mice caused by exposure of heavy metal cadmium, and has great application prospects.

The invention provides a children formula food capable of improving visual function and a preparation method and application thereof, the children formula food contains calcium and galactose, and the mass ratio of calcium to galactose is 1: (1-18); wherein in terms of dry matter of the infant formula food, the total protein content is 7.8 g to 30 g / 100 g, the fat content is 10 g to 35 g / 100 g, and the carbohydrate content is 37.5 g to 79.3 g / 100 g. The children formula food disclosed by the invention is beneficial to improving the visual function.

The invention relates to the technical field of food processing, and discloses a preparation method of bean curd with a double-network structure, and the bean curd with the double-network structure is prepared from the following raw materials in parts by weight: a recombinant protein matrix and a composite coagulator, the recombinant protein matrix is an aqueous dispersion with the total protein concentration of 10% and contains 7S globulin and 11S globulin, and the mass ratio of the 7S globulin to the 11S globulin is (30: 70)-(35: 65); the final total ion concentration of the system is 0.15-0.25 mol / L due to the addition amount of the composite coagulator; the mass ratio of 7S globulin to 11S globulin in the recombinant protein matrix is 32: 68. By constructing a 7S / 11S double-network interpenetrating structure and performing variable-frequency ultrasonic coupling ion / pH gradient regulation and control, the high hardness, high elasticity and strong water binding capacity of the soybean protein gel are synergistically improved, and the dry matter utilization rate and the product homogeneity are improved.

The present disclosure provides a sample analyzer for analyzing a urine sample, a system, and a method. The sample analyzer comprises: a sample preparation device; an optical detection device, comprising an optical detector; and a controller, configured to: control the sample preparation device to prepare a first test solution from a urine sample to be tested and a colorimetric reagent, and control the optical detector to test the first test solution so as to obtain a first urine total protein test result; control the sample preparation device to prepare a second test solution from said urine sample and a turbidimetric reagent, and control the optical detector to test the second test solution so as to obtain a second urine total protein test result; when said urine sample is proteinuria, on the basis of the first urine total protein test result and the second urine total protein test result, calculate a kidney injury marking parameter used for characterizing the kidney injury state of a subject; and output the kidney injury marking parameter.

This invention provides cheeses with increased protein content without increasing hardness, a method for producing the same, and a method for controlling the hardness of cheeses. [Solution] The above problem can be solved by having cheeses that contain dairy raw materials including raw cheese, a plant protein material, molten salt, and water, with a total protein content of 16% by mass or more and 28% by mass or less, and a mass ratio of plant protein to total protein of 0.03 or more and 0.32 or less. The cheeses can be manufactured by a manufacturing method comprising a raw material preparation step of preparing a mixture containing dairy raw materials including raw cheese, a plant protein material, molten salt, and water, and a heating and stirring step of heating and stirring the mixture, wherein the total protein content of the cheeses is 16% by mass or more and 28% by mass or less, and the mass ratio of plant protein to total protein is 0.03 or more and 0.32 or less.

Heat-treated, shelf-stable liquid nutritional compositions comprising whey protein and non-whey protein, and methods of producing and using these compositions. The compositions have a pH of from about 6.0 to about 8.0, a total protein content of at least about 6 g per 100 mL of the composition. The whey protein comprises or is provided by an ingredient that comprises heat-denaturable protein of which at least about 55% is present in a denatured state, and the non-whey protein comprises or consists of casein, or one or more non-dairy proteins, or casein and one or more non-dairy proteins.