81 results about "Protein total" patented technology

The invention discloses a composite microecological preparation as well as a preparation method and application thereof. The compound microecological preparation disclosed by the invention comprises lactobacillus casei, lactobacillus plantarum and saccharomyces cerevisiae. The application effect of the composite microecological preparation in cow breeding is remarkable, the optimal addition amount of the composite microecological preparation is 100 mL / cow / day, the production performance of lactating cows can be improved, the milk yield is improved by 3.30 kg / cow / day, the milk yield of 4% standard milk is improved by 3.78 kg / cow / day, the total protein is improved by 15.9%, the albumin is improved by 14.7%, the globulin is improved by 16.5%, and the somatic cell number is reduced by 52.6%; and pollutant emission can be reduced, including 37.2% of ammonia emission reduction and 28.7% of carbon dioxide emission reduction.

The invention discloses a protein extracting solution and a protein extracting method of polysaccharide and polyphenol plant tissues, and is characterized in that the protein extracting solution comprises the following components in molar ratio: 180-250 parts of trihydroxymethyl aminomethane hydrochloride buffer solution, 200-300 parts of sugar, 45-55 parts of chlorate, 10-70 parts of beta-mercaptoethanol and 0.5-2 parts of protease inhibitor. The browning condition of the total protein solution obtained by the protein extraction formula is obviously weakened, which indicates that the oxidation of polyphenol is obviously inhibited, the release of protein in a tissue sample is facilitated, and the extracted protein effect is better.

The invention belongs to the technical field of protein interaction prediction, and discloses a protein interaction prediction method and system based on dynamic graph attention, and the method comprises the following steps: 1, constructing a protein total graph; step 2, graph node feature enhancement: adjusting feature representation of residue nodes in the protein graph by using a gating mechanism, GRU time sequence updating and an additive attention mechanism, and performing adaptive enhancement on residue node features; step 3, predicting protein-protein interaction: aggregating information from adjacent nodes by adopting a dynamic graph attention mechanism, and capturing local and global interaction between residues; and splicing the output features of the two protein maps, and generating a score for predicting protein-protein interaction after passing through a full connection layer. According to the method, more accurate and stable protein interaction prediction is realized through dynamic feature fusion of a double-protein map structure.

The invention discloses a transcription factor GPC6 for regulating and controlling the protein content of rice grains and application of the transcription factor GPC6. The amino acid sequence of the transcription factor is shown as SEQ ID NO.1. The transcription factor comprises a genome region and a promoter, the nucleotide sequence of the genome region is shown as SEQ ID NO.2, the nucleotide sequence of the promoter is shown as SEQ ID NO.3, the coding sequence (CDS) is shown as SEQ ID NO.4, and the coding sequence (CDS) is shown as SEQ ID NO.3. The transcription factor GPC6 can be used for regulating and controlling the protein content of the rice grains. And comprises all alleles thereof. Whole genome association analysis is carried out on the glutelin content and the total protein content, and a site GPC6 capable of being repeatedly detected is identified on a No.6 chromosome. Gene cloning is carried out, gene resources for rice quality genetic improvement can be enriched, and help is provided for rice quality improvement, especially protein content improvement.

The application discloses a transcription factor for regulating protein content of rice grains GPC6 and application, the amino acid sequence of the transcription factor is shown as SEQ ID NO. 1, the transcription factor comprises a genomic region and a promoter, the nucleotide sequence of the genomic region is shown as SEQ ID NO. 2, the nucleotide sequence of the promoter is shown as SEQ ID NO. 3, the coding sequence (CDS) is shown as SEQ ID NO. 4, and all alleles thereof are contained. The application identifies a repeatedly detectable locus on chromosome 6 by performing whole genome association analysis on gluten content and total protein content GPC6 . Gene cloning is carried out on the locus, so that the genetic resources for improving rice quality are enriched, and help is provided for improving rice quality, in particular, protein content.

To provide fermented milk which is high in protein but has low hardness and does not harden during storage, and a manufacturing method of the same.SOLUTION: The above-mentioned problems can be solved by making a protein content of fermented milk 8.0 wt.% or more based on the total weight, and making the weight ratio of the protein derived from a microparticulated whey protein material to the total protein 0.50 or more. The fermented milk of the present invention has a hardness of less than 50 gf. The microparticulated whey protein material used in the fermented milk of the present invention has (a) a weight ratio of the protein to the total solid of less than 0.60, (b) a 50% particle size (D50) of 0.5 to 10 μm, and (c) a modification degree of the whey protein of 60% or more.SELECTED DRAWING: Figure 1

The invention relates to a process for dephosphorylation of skim milk, ultra-filtered milk (UF milk) or micellar casein isolate (MCI) comprising (i) acidification of a skim milk, UF milk or MCI preferably not lower than pH 6.0, preferably between 6.0 and 6.7, (ii) cooling the acidified skim milk, UF milk or MCI to the temperature between 0° C. and 15° C., (iii) adding gluconate and / or maleate to the cooled skim milk, UF milk or MCI and, (iv) washing the skim milk, UF milk or MCI, to remove phosphorus, thus dephosphorylating the skim milk, UF milk or MCI, preferably to an extent that the total phosphorous content of the skim milk, UF milk or MCI is reduced with at least 20%, preferably 20-40%, more preferably 30-40% compared to the material provided to step (i). The invention also relates to dephosphorylated MPC, MPI or MCI obtainable by the process of the invention, and to a liquid heat-sterilized enteral nutritional composition comprising 2.0-3.0 kcal / ml wherein 16-35 en % is provided by protein, the combination of caloric content and relative protein caloric content selected such that there is 10-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein in the composition, wherein the protein comprises micellar casein (MC), whey protein (WP) and optionally caseinate (CAS), wherein there is at least 70 wt % MC and less than 15 wt % WP, based on total protein content, and wherein the composition has a total amount of phosphorous less than 192 mg / 100 ml and / or 30-80 mg / 100 kcal, preferably at least 30-80 mg / 100 kcal, most preferably less than 192 mg / 100 ml and 30-80 mg / 100 kcal.

The present invention relates to a method for producing a lactoferrin-containing product by performing cation exchange with milk having a lactoferrin concentration of at least 4.0 per gram of protein. The invention also relates to a lactoferrin-containing product containing at least 96 wt% of lactoferrin and less than 0.60 wt% of immunoglobulin (IgG + IgA + IgM), based on the total protein.

Disclosed in the present invention are a ketamine small molecule probe, and a preparation method therefor and the use thereof. Two small molecular probes are designed and synthesized, and the blank in the prior art is filled. A target fishing experiment is performed on the total protein in a cell lysis buffer by means of using the two probes, and 61 and 70 target proteins are obtained, respectively. In addition, more than three technical repetitions verify that the two probes can stably bind to the target proteins. A screened SIRT2 protein is used as a target protein of ketamine, and a reference basis is provided for the synthesis and preparation of an anti-inflammatory related drug.

To provide a nutritive composition for infants having an amino acid composition more similar to breast milk, making it possible to ensure sufficient amino acid nutrition without excessive ingestion of protein.SOLUTION: The present disclosure provides a nutritive composition for infants containing protein. In the composition, the total content of phenylalanine and tyrosine is 7.6 wt.% or more in terms of total amino acid content, and 90 wt.% or more of the total protein content is derived from milk.SELECTED DRAWING: Figure 1

The present invention relates to a process for the dephosphorylation of skimmed milk, ultra-filtered milk (UF milk) or micellar casein isolate (MCI) comprising (i) acidifying the skimmed milk, UF milk or MCI, preferably not below pH 6.0, preferably between 6.0 and 6.7, (ii) cooling the acidified skimmed milk, UF milk or MCI to a temperature between 0 DEG C and 15 DEG C, (iii) adding gluconate and / or maleate to the cooled skimmed milk, UF milk or MCI, and (iv) washing the skimmed milk, UF milk or MCI to remove phosphorus, thereby dephosphorylating the skimmed milk, UF milk or MCI, preferably to the extent that the total phosphorus content of the skimmed milk, UF milk or MCI is reduced by at least 20%, preferably 20-40%, more preferably 30-40%, compared to the material provided to step (i). The invention also relates to a dephosphorylated MPC, MPI or MCI obtainable by the process of the invention and to a liquid enteral nutritional composition comprising 2.0-3.0 kcal / ml of heat sterilization wherein 16 en%-35 en% is provided by a protein, the combination of the calorie content and the relative protein calorie content being selected such that 10-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein is present in the composition, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein. Wherein the protein comprises micellar casein (MC), whey protein (WP) and optionally caseinate (CAS) wherein at least 70 wt% MC and less than 15 wt% WP are present, based on the total protein content, and wherein the composition has a total amount of phosphorus of less than 192 mg / 100 ml and / or 30-80 mg / 100 kcal, preferably at least 30-80 mg / 100 kcal, most preferably less than 192 mg / 100 ml and 30-80 mg / 100 kcal.

The present invention relates to the field of nutritional compositions, methods of providing nutritional compositions and uses thereof. More particularly, the present invention relates to a ready-to-eat powdered infant formula or ready-to-eat powdered growth formula exhibiting desirable gastric digestion characteristics. There is provided a nutritional composition comprising casein, whey protein comprising alpha-lactalbumin (aLac) and beta-lactoglobulin (bLac), and protein-coated fat droplets wherein the composition has a protein content of from 5% to 20% (w / w) based on total solids; the bLac content is at least 3% (w / w) based on the total protein level, and the aLac + bLac content is at least 25% (w / w) based on the total protein level; the fat content is in the range of 10% to 50% (w / w) based on total solids; the fat droplets are coated with an average protein load of 2 to 10 mg / m2, and wherein the weight ratio of casein: (aLacc + bLac) on the fat droplets is at least 4 times the weight ratio of casein: (aLacc + bLac) in the total composition; the level of sphingomyelin per 100 g of the composition is between 70 mg and 200 mg; the level of phospholipids per 100 g of the composition is between 280 mg and 950 mg.

To provide a beer taste non-alcohol beverage that is excellent in flavor (for example, taste thickness, bitterness, and harshness) and in foam keeping.SOLUTION: A beer taste beverage has an alcohol content of less than 1 v / v%, a total polyphenol amount of 100 mg / L or more, a total protein amount of more than 0 μg / mL and 100 μg / mL or less, and an NIBEM value of 250 s or more.SELECTED DRAWING: None

[Problem] There is a demand for a protein-rich food having excellent protein quality and quantity and a texture required in the market, and having excellent flavor. [Solution] The problem is solved by providing a protein-rich food having a total protein content of 10 g or more per 100 g and contains (A) a soybean protein and (B) a micellar casein protein, wherein the content of the soybean protein to the total protein mass is 2-70 mass%.

The present invention proposes a dry chemical reagent sheet for quantitatively detecting total protein and a preparation method thereof, and relates to the field of chemical reagent sheets. The dry chemical reagent sheet for quantitatively detecting total protein comprises a support layer, a reagent layer and a diffusion layer, wherein the reagent layer is arranged above the support layer, the diffusion layer is arranged above the reagent layer, the diffusion layer comprises a diffusion layer reagent or is formed by a diffusion layer reagent, the diffusion layer reagent comprises an adhesive, a surfactant and particulate matter, and the particulate matter comprises a first particulate matter and a second particulate matter; the number ratio of the first particulate matter to the second particulate matter is 1:(0.25-4), and the difference in particle size between the first particulate matter and the second particulate matter is 10-40 μm. The dry sheet for quantitative detection of total protein provided by the present invention is used for in vitro diagnosis, and its detection result is accurate, storage is stable, and it has the advantages of rapid diagnosis, simple operation, cost saving, etc.

The invention relates to a swimming bladder hydrolysate capable of improving a male reproductive function as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention provides a swimming bladder hydrolysate capable of improving a male reproductive function. The swimming bladder hydrolysate is obtained by performing enzymolysis on sturgeon swimming bladders through trypsin. The total protein content of the swimming bladder hydrolysate is 80%-90%; the amino acid composition of the swimming bladder hydrolysate comprises glycine, proline, glutamic acid, asparaginic acid, arginine, alanine, serine, lysine, threonine, phenylalanine, leucine, isoleucine, valine, histidine, tyrosine and methionine. Researches show that the swim bladder hydrolysate has very strong antioxidant activity, and the swim bladder hydrolysate can effectively relieve matt hair, unstable spirit, reduced exploration behavior, weight loss and male reproductive function damage of model mice caused by exposure of heavy metal cadmium, and has great application prospects.

The invention provides a children formula food capable of improving visual function and a preparation method and application thereof, the children formula food contains calcium and galactose, and the mass ratio of calcium to galactose is 1: (1-18); wherein in terms of dry matter of the infant formula food, the total protein content is 7.8 g to 30 g / 100 g, the fat content is 10 g to 35 g / 100 g, and the carbohydrate content is 37.5 g to 79.3 g / 100 g. The children formula food disclosed by the invention is beneficial to improving the visual function.

The invention relates to the technical field of food processing, and discloses a preparation method of bean curd with a double-network structure, and the bean curd with the double-network structure is prepared from the following raw materials in parts by weight: a recombinant protein matrix and a composite coagulator, the recombinant protein matrix is an aqueous dispersion with the total protein concentration of 10% and contains 7S globulin and 11S globulin, and the mass ratio of the 7S globulin to the 11S globulin is (30: 70)-(35: 65); the final total ion concentration of the system is 0.15-0.25 mol / L due to the addition amount of the composite coagulator; the mass ratio of 7S globulin to 11S globulin in the recombinant protein matrix is 32: 68. By constructing a 7S / 11S double-network interpenetrating structure and performing variable-frequency ultrasonic coupling ion / pH gradient regulation and control, the high hardness, high elasticity and strong water binding capacity of the soybean protein gel are synergistically improved, and the dry matter utilization rate and the product homogeneity are improved.

The present disclosure provides a sample analyzer for analyzing a urine sample, a system, and a method. The sample analyzer comprises: a sample preparation device; an optical detection device, comprising an optical detector; and a controller, configured to: control the sample preparation device to prepare a first test solution from a urine sample to be tested and a colorimetric reagent, and control the optical detector to test the first test solution so as to obtain a first urine total protein test result; control the sample preparation device to prepare a second test solution from said urine sample and a turbidimetric reagent, and control the optical detector to test the second test solution so as to obtain a second urine total protein test result; when said urine sample is proteinuria, on the basis of the first urine total protein test result and the second urine total protein test result, calculate a kidney injury marking parameter used for characterizing the kidney injury state of a subject; and output the kidney injury marking parameter.

This invention provides cheeses with increased protein content without increasing hardness, a method for producing the same, and a method for controlling the hardness of cheeses. [Solution] The above problem can be solved by having cheeses that contain dairy raw materials including raw cheese, a plant protein material, molten salt, and water, with a total protein content of 16% by mass or more and 28% by mass or less, and a mass ratio of plant protein to total protein of 0.03 or more and 0.32 or less. The cheeses can be manufactured by a manufacturing method comprising a raw material preparation step of preparing a mixture containing dairy raw materials including raw cheese, a plant protein material, molten salt, and water, and a heating and stirring step of heating and stirring the mixture, wherein the total protein content of the cheeses is 16% by mass or more and 28% by mass or less, and the mass ratio of plant protein to total protein is 0.03 or more and 0.32 or less.

Heat-treated, shelf-stable liquid nutritional compositions comprising whey protein and non-whey protein, and methods of producing and using these compositions. The compositions have a pH of from about 6.0 to about 8.0, a total protein content of at least about 6 g per 100 mL of the composition. The whey protein comprises or is provided by an ingredient that comprises heat-denaturable protein of which at least about 55% is present in a denatured state, and the non-whey protein comprises or consists of casein, or one or more non-dairy proteins, or casein and one or more non-dairy proteins.

The invention provides meat protein liquid food suitable for people with dysphagia and a preparation method of the meat protein liquid food, and relates to the field of food. The meat protein liquid food comprises the following raw materials: a protein solution and a thickening agent, the protein solution contains meat protein and whey protein, the total protein concentration is 6-8 wt%, and the mass ratio of the meat protein to the whey protein is 1: (1-3); the thickening agent comprises the following components in percentage by mass: 0.25%-0.75% of sodium carboxymethyl cellulose, 0.25%-0.75% of xanthan gum and 0.25%-0.75% of inulin, and the sum of the mass concentrations of the sodium carboxymethyl cellulose, the xanthan gum and the inulin is 1.25%-1.75%. The meat protein liquid diet provided by the invention is high in total protein concentration, can effectively make up for insufficient protein intake caused by degeneration of chewing and swallowing functions of old people, and prevents muscle atrophy and malnutrition; the prepared meat protein liquid food reaches IDDSI level 3 or level 4, and the risks of food residues and aspiration in the swallowing process can be effectively reduced.

To provide an instant soup composition or the like containing protein at a high concentration and having improved texture of soup.SOLUTION: A solid instant soup composition comprising (A) a particulate insoluble proteinaceous material and (B) water-soluble collagen, wherein a weight ratio (A: B) of a content of the (A) to a content of the (B) is 1:0.18 to 1:1.5, and a total protein amount is 40 to 75% by weight.SELECTED DRAWING: None

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica with high content of Cys, Met and His as well as a construction method and application of the yarrowia lipolytica. Specifically, the yarrowia lipolytica strain with high Cys, Met and His contents is successfully constructed through a genetic engineering means, the comprehensive content of three target amino acids accounts for 3.0% or above of the dry cell weight, meanwhile, the construction method of the strain is simple and efficient, the content of the target amino acids can be remarkably increased through overexpression of a single gene, and industrial popularization is easy. The total protein content of the strain is kept at a higher level of 40%-65%, and the prepared single-cell protein is excellent in nutritional value and wide in application prospect by combining with rich target amino acids.

The invention discloses a method for increasing protein content and amino acid content of phaeodactylum tricornutum and application. According to the method, a DPH gene in wild type phaeodactylum tricornutum is knocked out through a gene knockout technology, so that the total protein content and the amino acid content of the phaeodactylum tricornutum are increased, a method for knocking out the DPH gene in the wild type phaeodactylum tricornutum comprises the steps that the DPH gene is knocked out in a targeted mode through a CRISPR / Cas9 editing carrier, the nucleotide sequence of gRNA of the DPH gene is knocked out in a targeted mode through the CRISPR / Cas9 editing carrier is shown as SEQ ID No.3, and the content of the total protein in the wild type phaeodactylum tricornutum is increased. The total protein content and the essential amino acid content of the DPH gene knockout phaeodactylum tricornutum strain are remarkably improved compared with those of a wild type strain. The transformed algal strain can be used as a high-quality protein source to be applied to the field of aquaculture, the nutritional value and the transformation efficiency of feed are effectively improved, and the growth performance and the health condition of bred animals are improved. Wide market prospects and important economic values are realized.

The present application provides a preparation method of human serum albumin, which comprises inoculating human serum albumin-producing Pichia pastoris into a culture medium for fermentation, and obtaining human serum albumin from the fermentation liquor; the culture medium is BSM culture medium with the concentration of all components reduced to at most 1 / 4 except for two components of glycerol and Pichia pastoris trace elements 1, preferably reduced to 1 / 4-1 / 2. The present application also provides a purification method of human serum albumin. The yield and the proportion of human serum albumin in total protein obtained by the preparation method of the present application are significantly higher than those of the prior art, the cost of the culture medium used in the preparation method is significantly reduced, and there is no risk of animal-derived virus contamination, thereby significantly reducing the cost of the preparation method. The recovery rate is significantly improved by using the purification method of the present application, and the purity of the final product is also significantly further improved.

The invention discloses an application of a rice phosphorus transporter gene and an encoding protein thereof in improving nutrient utilization efficiency and rice quality. According to the invention, OsPHT1 is mutated or inhibited; 1 gene expression can significantly improve nutrient utilization efficiency and rice taste quality and nutritional quality. The method is mainly embodied in that accumulation of nitrogen and phosphorus nutrients of rice is remarkably reduced, the total protein content, glutelin content, prolamin content, globulin content, albumin content and phytic acid content of rice grains are remarkably reduced, the gel consistency of the rice is increased, the reduction value is reduced, and the eating quality and the nutritional quality of the rice are further improved.

A lyophilized composition comprising a polyclonal immunoglobulin M (IgM) comprising the steps of: a) providing an initial aqueous solution comprising IgM at a concentration of from about 10 mg / mL to about 50 mg / mL, the polyclonal IgM being at least about 90% by weight of the total protein content in the composition; b) adding an amino acid selected from the group consisting of proline, glycine, alanine, valine and hydroxyproline, or mixtures thereof, at a final concentration of from about 0.15 M to about 0.45 M, polysorbate 80 (PS80) at a concentration of from about 50 to about 200 ppm, and succinic acid at a concentration of from about 1 mM to about 20 mM; and c) lyophilizing the IgM composition obtained in the step b), wherein the content of pentamer IgM in the lyophilized composition is greater than about 90% of the total IgM content, and the content of IgM aggregate is less than about 1.5%.

The invention provides modified milk powder capable of relieving chronic gastritis as well as a preparation method and application of the modified milk powder. The modified milk powder disclosed by the invention has the total protein content of 16.5-30 g / 100g, the fat content of 1.0-26 g / 100g, the dietary fiber content of 0-15.0 g / 100g and the carbon-water content of 30-69 g / 100g. Moreover, the modified milk powder contains hericium erinaceus and / or an extract thereof and dried ginger and / or an extract thereof, the weight ratio of the sum of the hericium erinaceus and the extract thereof to the sum of the dried ginger and the extract thereof is 1: (0.2-4) according to the amount of dry matter, and the total content of the hericium erinaceus and / or the extract thereof and the dried ginger and / or the extract thereof in the modified milk powder is 0.1-2 g / 100 g. The modified milk powder can relieve chronic gastritis and improve gastric mucosal lesion.

A liquid egg substitute composition containing a plant protein and a heat-setting gelling agent, the liquid egg substitute composition having a total protein content of 8-15% by mass (inclusive), when the protein contained in the supernatant when the liquid egg substitute composition is centrifuged for 15 minutes at a centrifugal force of 8000 * g is used as the supernatant protein, the ratio of the content of the supernatant protein to the total protein content is 6-21%.