119 results about "Protein total" patented technology

The invention relates to a whey protein product having a ratio of whey protein to casein in the range from about 90:10 to about 50:50 and the total protein content of at least 20% on dry matter basis, and a method for its preparation. The product has a favourable amino acid composition and is especially suitable for athletes.

The invention relates to a dimensional electrophoresis method for a total protein of a jute root system, belonging to the field of biotechnology. The total protein of the jute root system capable of existing in the coastal beach is separated by the dimensional electrophoresis technique, and by adopting the technical scheme, the jute root system existing in the coastal beach is tested with good effect. At present, repeated experiments prove that the jute root system is separated by the dimensional electrophoresis method to obtain more protein points; and after detected by PDQuest software, the protein points are more than 1100, the map is clear without transverse and longitudinal grains, the protein points are circular, and the repeatability is good, thus the invention is a dimensional electrophoresis method suitable for the analysis of the total proteomics of the jute root system.

The present invention relates to pharmaceutical compositions comprising MBL and/or MBL variants. In particular the invention relates to pharmaceutical compositions comprising at least 200 mug/ml protein containing material, wherein mannan binding lectin (MBL) and/or MBL variants constitutes at least 35% (w/w) of the total protein; or to compositions comprising at least 400 mug/ml mannan binding lectin (MBL) and/or MBL variants. In addition the invention relates to pharmaceutical compositions comprising MBL and/or MBL variants and divalent cations. The invention also describes methods of preparing said compositions. The pharmaceutical compositions according to the invention may for example be used in methods of treatment of a number of different clinical conditions including infections. Uses of the compositions for preparation of medicaments for treatment of a clinical condition are also described.

Disclosed are flavored nutritional beverages comprising (A) fat; (B) milk protein representing from about 10% to 100% by weight of total protein; (C) carbohydrate comprising from about 75% to 100% by weight of at least one of (i) from about 0.1% to about 10% sucrose, trehalose, or combination thereof, by weight of the beverage, and (iii) from about 0.1% to about 20% maltodextrin by weight of the nutritional liquid, the maltodextrin having a DE value of from about 1 to about 10, and (iii) combinations of (i) and (ii); (D) an iron-containing material, and (E) a flavorant. The flavored nutritional beverages, unlike many iron-fortified milk-based beverages available today, do not readily develop beige or gray hues during formulation, processing and storage, and are thus more easily formulated with little or no color distortion and with improved or more accurately matched flavor-color combinations.

The present invention relates to one kind of yak bone marrow protein polypeptide osseine comprising protein total nitrogen 13-15.8 wt%, free amino acid accounting in nitrogen 1-3 wt%, calcium 0.1-1 wt%, and total phosphorus 0.01-0.1 wt%. The yak bone marrow protein polypeptide osseine contains protein over 80 wt% and various kinds of polypeptide and amino acid. It has simple preparation process and low cost. It may be prepared into composition via adding plant additive and the composition has improved taste, hydroscopicity and flowability. The present invention can provide human body with protein, polypeptides and amino acids.

The invention discloses a procaryotic cell non-merge solubility pressing system, which comprises the following steps: building one type auxiliary protein gene or two type auxiliary protein gene and pre-expressing exogenesis goal gene in a transcription unit; proceeding intracellular co-express through each self-contained translational control system of auxiliary protein gene and pre-expressing exogenesis goal gene in the same pronucleus expression host cell; utilizing the auxiliary protein to realize non-merge solubility expression especially to realize non-merge solubility expression with multiple disulfide bond peptide chain. This system can make the expressing quantity of procaryotic cell occupy above 105 of thallus total protein under the normal condition.

The present invention provides a high quality, ready-to-drink, non-cultured nutritional beverage comprising (1) a protein component, (2) an emulsified fat component, (3) a fortification component, and (4) an optional sweetener component. Preferably the protein component comprises minimally processed milk; the emulsified fat component comprises dairy fat; the beverage contains about 1.5 to 10 percent total protein and about 0.15 to about 5 percent total fat; the beverage has a first ratio of the minimally processed milk protein divided by the total protein which is greater than or equal to about 0.05; the beverage has a second ratio of the dairy fat divided by the total fat which is greater than or equal to about 0.15; and the fortification component provides at least 10 percent of the daily value per single beverage serving of at least 6 vitamins and minerals.

The invention provides maternized formula milk powder containing nucleotide for 0-12 month infants and a preparation method thereof. Every 100g of the milk powder contains 10.5-18g total protein, 1.25-1.8g of alpha-whey protein, 2.2-3.7g of beta-casein, 35-60mg of lactoferrin, 20-29g of fat, 2600-4500mg of linoleic acid, 350-450mg of alpha-linolenic acid, 52-56g of carbohydrate and 26-58mg of total nucleotides by the total molecular weight of nucleotide, wherein whey protein is 48-61 percent of the weight of the total protein. The milk is close to breast milk, and can be used for improving the immunity of infants.

The invention relates to an extraction method of protein from animal tissues, in particular to an extraction method of total protein from a rumen epithelial tissue of a milk cow. The method comprises the steps of putting a rumen epithelial tissue sample of the milk cow in a precooled mortar, adding liquid nitrogen, grinding into powder, adding an acetone solution of precooled trichloroacetic acid, oscillating, eddying, uniformly mixing for overnight aging, centrifugally collecting sediment, freeze-drying the tissue sediment, adding lysate, incubating and eddying at intervals, and centrifugally collecting supernate, namely the total protein of the rumen epithelial tissue of the milk cow. For the total protein of the rumen epithelial tissue of the milk cow, extracted by the method, a total protein mixture can be separated by a two-dimensional gel electrophoresis technology according to an isoelectric point and molecular weight of the protein, the relative abundance of the protein is acquired, the isoelectric point, the molecular weight and a relative transcript level of each protein can be clearly and visually shown in a gel atlas, and the method has the advantages of more obvious integrity and visualization in comparison with the prior arts such as a high efficiency liquid chromatography separation method, an enzyme-linked immunosorbent assay and immunoblotting.

The invention discloses method for detecting and evaluating quality of peanuts suitable for soluble protein processing. A method for detecting the quality of the peanuts suitable for the soluble protein processing comprises the following steps of: detecting the following indexes of a peanut sample, such as crude fat content, total protein content, total sugar content, cystine content, arginine content, content of conarrachin I, mass percentage content of a subunit with molecular weight of 37.5kDa based on total protein, the mass percentage content of the subunit with the molecular weight of 23.5kDa based on the total protein, the mass percentage content of the subunit with the molecular weight of 15.5kDa based on the total protein, a protein extraction ratio and a kernel ratio; and substituting measured values into a formula (1) so as to obtain dissolubility of the peanut sample. The invention also provides a method for evaluating the quality of the peanuts suitable for the soluble protein processing, namely, detecting the dissolubility of the peanut sample to be detected according to the method, and classifying the peanut sample to be detected according to standards of the following 1) to 3): 1) if a calculated value of the dissolubility is not less than 86, the peanut sample is suitable for the soluble protein processing; 2) if the calculated value of the dissolubility ranges from 68-86, the peanut sample is substantially suitable for the soluble protein processing; and 3) if the calculated value of the dissolubility is not more than 86, the peanut sample is not suitable for the soluble protein processing.

The invention provides a purification method for split influenza virus vaccine. An influenza virus strain is inoculated onto a chick embryo and cultured to obtain a virus solution; the virus solution is sequentially subjected to inactivation and ultrafiltration concentration to obtain an ultrafiltrate; the obtained ultrafiltrate is subjected to cane sugar density gradient centrifugation by using a KII continuous flow centrifuge to obtain an ultra centrifugal solution; the ultra centrifugal solution is subjected to ultrafiltration dialysis for cane sugar removal and molecular sieve gel chromatography to obtain a virus purification solution; the obtained virus purification solution is subjected to virus split by using a split agent TritonX-100 and sodium deoxycholate; after the split is over, the split agent is removed via ultrafiltration dialysis; impure protein is centrifugally removed from the obtained split solution; supernatant is collected, filtered and sterilized so as to obtain the purified influenza virus vaccine primary solution. The purification method is simple and convenient to operate, high in centrifugation capacity and suitable for large-scale production; by adopting a dual-split agent and a centrifugal process, the finished product is greatly improved in activity (hemagglutinin content/protein total content), and approaches the activity ratio of subunit vaccine, as a result, the high-grade split influenza virus vaccine product is obtained.

The invention provides a method for producing yeast cells by fermenting a protein and impurity removed soybean liquid. A liquid left after separating soybean proteins from degreased soybeans and centrifuging for removing impurities is called as the protein and impurity removed soybean liquid. The protein and impurity removed soybean liquid also contains large amounts of low-molecular-weight proteins, carbohydrates, peptides and the like, and the method for producing the yeast cells by fermenting the protein and impurity removed soybean liquid allows the total protein yield of a single yeast to be 2.42g/L, the COD to be obviously reduced, the COD removal rate to reach 40%, and substantially solves resource waste and environmental pollution problems.

The invention belongs to the field of tobacco gene engineering, and particularly relates to a regulatory gene tobacco lycopene epsilon-cyclase gene Nt epsilon-LCY2 for reducing total protein of tobacco leaves and phenol content in smoke. The base sequence of the gene is shown as SEQ ID NO.1. The tobacco lycopene epsilon-cyclase Nt epsilon-LCY2 is composed of 498 amino acid residues, wherein the 105 to 287 amino acid and the 290 to 475 amino acid are conservative transport protein structural domains. The protein is related to the total protein content in plant leaves, and after the gene expression is reduced, the total protein content in the leaves is obviously reduced. Preliminary research on the specific Nt epsilon-LCY2 shows that the specific Nt epsilon-LCY2 is highly related to the total protein content of the tobacco, and after the gene is mutated, the total protein content of the tobacco is obviously reduced. On the basis of the characteristic, a certain application basis and reference can be provided for the cultivation of new varieties of low-phenol-content tobaccos.

The invention relates to a fluorescent microsphere labeled antibody as well as a preparation method and application thereof. The preparation method comprises the following steps: adding 100-1000mg/mLof a chemical cross-linking agent solution into 1-50mg/mL of a fluorescent microsphere solution for activation; mixing the labeled antibody with quality control protein to obtain total protein, and adding the total protein into the obtained activated product for reaction; and adding a sealing agent into the obtained solution for sealing, and centrifuging to obtain the fluorescent microsphere labeled antibody. The labeled antibody/antigen and other proteins are simultaneously labeled on the surface of the marker, thereby lowering the consumption of the labeled antibody/antigen and lowering thecost; and moreover, the original performance of the labeled antibody/antigen can be kept good and stable.

The invention relates to a method and system for washing corn starch by reduced swirler separation. A three-stage diversion swirl system I is used for diverting crude starch milk, the protein solution from the top flow enters a three-stage diversion swirl system II, and through the three-stage diversion swirl system II, corn protein powder with the total protein content of 60-63% can be obtained only by refluxing instead of water treatment of the centrifuge. The invention uses 1.5 times of washing water consumption of the refluxing swirl method to achieve the goal of reducing the stages of swirlers on the premise of ensuring the washing effect. The invention greatly shortens the swirl washing time and the energy consumption of the swirl system; and the whole technique can shorten the swirl washing step time by 80% and reduce the energy for the swirl system by 72%.

The invention discloses a method for detecting transgenic ingredients in food. The method comprises the steps of step I. extracting total proteins of a raw material; step II. injecting the total protein obtained in the step I into a detection device, and enabling the total protein to pass through the detection device, wherein the detection device comprises a sample feeding device, a plurality of hollow fiber polypropylene films and a first porous plate body, each hollow fiber polypropylene film is provided with a first antibody which is specifically combined with the transgenic protein in an attaching manner, the sample feeding device comprises a second porous plate body and a cone, and two ends of each hollow fiber polypropylene film are respectively fixed on the first porous plate body and the second porous plate body and are respectively communicated with holes in the first and second porous plate bodies; step III. washing; step IV. injecting a second antibody which is specifically combined with the transgenic protein and provided with marks into sample feeding holes of the detection device, and enabling the second antibody to pass through the detection device; step V. washing; and step VI, detecting whether the hollow fiber polypropylene film has the mark or not, and correlating the detection result with whether the transgenic ingredient exists in the raw material.