133 results about "Protein total" patented technology

The invention relates to a whey protein product having a ratio of whey protein to casein in the range from about 90:10 to about 50:50 and the total protein content of at least 20% on dry matter basis, and a method for its preparation. The product has a favourable amino acid composition and is especially suitable for athletes.

The invention relates to a dimensional electrophoresis method for a total protein of a jute root system, belonging to the field of biotechnology. The total protein of the jute root system capable of existing in the coastal beach is separated by the dimensional electrophoresis technique, and by adopting the technical scheme, the jute root system existing in the coastal beach is tested with good effect. At present, repeated experiments prove that the jute root system is separated by the dimensional electrophoresis method to obtain more protein points; and after detected by PDQuest software, the protein points are more than 1100, the map is clear without transverse and longitudinal grains, the protein points are circular, and the repeatability is good, thus the invention is a dimensional electrophoresis method suitable for the analysis of the total proteomics of the jute root system.

A method is disclosed for producing an infant formula base, wherein by means of microfiltration, ultrafiltration, and nanofiltration the components of milk are separated into a casein fraction, a whey protein fraction, and a lactose fraction to produce, by suitably combining, a composition in which the amino acid composition is close to that of human milk. An infant formula base having a total protein concentration of about 1.0 to about 1.5% and a beta-casein concentration of at least about 11 % of the total protein concentration is also disclosed. The infant formula base is suitable for the production of an infant formula.

The present invention relates to pharmaceutical compositions comprising MBL and / or MBL variants. In particular the invention relates to pharmaceutical compositions comprising at least 200 mug / ml protein containing material, wherein mannan binding lectin (MBL) and / or MBL variants constitutes at least 35% (w / w) of the total protein; or to compositions comprising at least 400 mug / ml mannan binding lectin (MBL) and / or MBL variants. In addition the invention relates to pharmaceutical compositions comprising MBL and / or MBL variants and divalent cations. The invention also describes methods of preparing said compositions. The pharmaceutical compositions according to the invention may for example be used in methods of treatment of a number of different clinical conditions including infections. Uses of the compositions for preparation of medicaments for treatment of a clinical condition are also described.

The invention is directed to seed and seed extract compositions containing levels of a human milk protein between 3-40% or higher of the total protein weight of the soluble protein extractable from the seed. Also disclosed is a method of producing the seed with high levels of extractable human milk protein. The method includes transforming a monocotyledonous plant with a chimeric gene having a protein-coding sequence encoding a protein normally present in human milk under the control of a seed maturation-specific promoter. The method may further includes a leader DNA sequence encoding a monocot seed-specific transit sequence capable to target a linked milk protein to a storage body.

Disclosed are flavored nutritional beverages comprising (A) fat; (B) milk protein representing from about 10% to 100% by weight of total protein; (C) carbohydrate comprising from about 75% to 100% by weight of at least one of (i) from about 0.1% to about 10% sucrose, trehalose, or combination thereof, by weight of the beverage, and (iii) from about 0.1% to about 20% maltodextrin by weight of the nutritional liquid, the maltodextrin having a DE value of from about 1 to about 10, and (iii) combinations of (i) and (ii); (D) an iron-containing material, and (E) a flavorant. The flavored nutritional beverages, unlike many iron-fortified milk-based beverages available today, do not readily develop beige or gray hues during formulation, processing and storage, and are thus more easily formulated with little or no color distortion and with improved or more accurately matched flavor-color combinations.

The invention discloses a continuous zymolysis synthesis technique of peanut protein active peptides and the preparation method thereof. The active peptides account for more than or equal to 90 percent of the total proteins, and the protein active peptides account for more than or equal to 80 percent of the total proteins, fat is less than equal to 1 percent, water is less than equal to 6 percent, ash is less than equal to 2 percent. The invention uses peanut protein as raw materials, and prepares peanut protein active peptide through continuous zymolysis synthesis technique, compared with powder protein, the peanut protein active peptides have better dissolubility, fluidity and stability, therefore, the peanut protein active peptides can be widely applied to such fields as medicine, everyday chemical and foods, etc.

This invention relates to a walnut oil composition, its extraction method and its application to intravements emulsion. Envery 100 ml emulsion of this invention contains walnut oil composition for injection 5-30 g, emulsifier 0.5-3.0g, isosmotic agent 0.5-3.5g and the rest being injection water. Beside that it can supplement nutritive energy for organism, resist fatigue, tolerate oxygen deficiency, raise immunity function and total protein action of serum, it also has a certain depression action for animal transplanted tumour, Lewis tumour, mouse HAC and concurrently has functions for invigorating kidney, warming lung and loosening the bowel.

The invention discloses an extraction and dielectrophoresis analytical method for total protein of cattail cauloid, comprising the following steps of: firstly, efficiently extracting protein from the cattail cauloid by screening through many experiments; under a preferred condition, separating the protein components from the secondary metabolite (such as pigments, phenols, quinones and the like) components by a dielectrophoresis method; and performing qualitative analysis by a silver nitrate staining method under a preferred condition, and / or performing qualitative and quantitative analysis on the separated protein by a mass spectrometer. Shown by experimental results, more protein spots are obtained by separation through the dielectrophoresis analytical method, and detected by PDQuest image analysis software, the number of the protein spots is more than 1000, and the spectrum is clear without transverse and longitudinal stripes; and the method has good repeatability and stability, can be used for conveniently identifying the authenticity of the pharmaceutical / food resource plant cattail, and provides a quality control standard and scientific base for in-depth study, scientific planting, culture, development and utilization of cattail.

The present invention relates to one kind of yak bone marrow protein polypeptide osseine comprising protein total nitrogen 13-15.8 wt%, free amino acid accounting in nitrogen 1-3 wt%, calcium 0.1-1 wt%, and total phosphorus 0.01-0.1 wt%. The yak bone marrow protein polypeptide osseine contains protein over 80 wt% and various kinds of polypeptide and amino acid. It has simple preparation process and low cost. It may be prepared into composition via adding plant additive and the composition has improved taste, hydroscopicity and flowability. The present invention can provide human body with protein, polypeptides and amino acids.

The present invention relates to an analysis method for a neuron injury related differentially expressed protein. The method comprises: 1, constructing a zebrafish brain and central nerve injury model, and grouping; 2, carrying out drug treatment on the drug group; 3, extracting the total protein in each group; 4, loading the sample onto a mass spectrometer, and analyzing; and 5, carrying out bioinformatics analysis.

The invention discloses a procaryotic cell non-merge solubility pressing system, which comprises the following steps: building one type auxiliary protein gene or two type auxiliary protein gene and pre-expressing exogenesis goal gene in a transcription unit; proceeding intracellular co-express through each self-contained translational control system of auxiliary protein gene and pre-expressing exogenesis goal gene in the same pronucleus expression host cell; utilizing the auxiliary protein to realize non-merge solubility expression especially to realize non-merge solubility expression with multiple disulfide bond peptide chain. This system can make the expressing quantity of procaryotic cell occupy above 105 of thallus total protein under the normal condition.

The present invention provides a high quality, ready-to-drink, non-cultured nutritional beverage comprising (1) a protein component, (2) an emulsified fat component, (3) a fortification component, and (4) an optional sweetener component. Preferably the protein component comprises minimally processed milk; the emulsified fat component comprises dairy fat; the beverage contains about 1.5 to 10 percent total protein and about 0.15 to about 5 percent total fat; the beverage has a first ratio of the minimally processed milk protein divided by the total protein which is greater than or equal to about 0.05; the beverage has a second ratio of the dairy fat divided by the total fat which is greater than or equal to about 0.15; and the fortification component provides at least 10 percent of the daily value per single beverage serving of at least 6 vitamins and minerals.

The invention provides maternized formula milk powder containing nucleotide for 0-12 month infants and a preparation method thereof. Every 100g of the milk powder contains 10.5-18g total protein, 1.25-1.8g of alpha-whey protein, 2.2-3.7g of beta-casein, 35-60mg of lactoferrin, 20-29g of fat, 2600-4500mg of linoleic acid, 350-450mg of alpha-linolenic acid, 52-56g of carbohydrate and 26-58mg of total nucleotides by the total molecular weight of nucleotide, wherein whey protein is 48-61 percent of the weight of the total protein. The milk is close to breast milk, and can be used for improving the immunity of infants.

A wheat bioactive peptide additive used for a cell culture medium is provided. The mass of oligopeptides having molecular weights lower than 1000 Dalton is not less than 90% of the total protein mass in the additive, and the oligopeptides at least comprise one or more of VN, TFN, QVSQ, HGAQ, GGDNP and GTCGAG. The additive can be blended with various base culture media and used for serum-free culture of a plurality of animal cells. The additive largely reduces the cost of the cell culture medium, reduces pollutions and other problems caused by animal-source components, promotes cell proliferation, improves cell viability and enhances expression of cell products.

The invention relates to a method for producing a milk-based product, comprising the steps of: providing a milk raw material; altering a ratio of casein to total protein of the milk raw material to less than about 0.80; subjecting the milk raw material with the altered ratio of casein to total protein from step b) to a heat treatment at the temperature of at least about 150° C. for a period of at most about 0.3 sec; cooling the heat-treated milk raw material from step c) to provide the milk-based product. The method provides milk-based products with long shelf life and good organoleptic properties.

The invention relates to an extraction method of protein from animal tissues, in particular to an extraction method of total protein from a rumen epithelial tissue of a milk cow. The method comprises the steps of putting a rumen epithelial tissue sample of the milk cow in a precooled mortar, adding liquid nitrogen, grinding into powder, adding an acetone solution of precooled trichloroacetic acid, oscillating, eddying, uniformly mixing for overnight aging, centrifugally collecting sediment, freeze-drying the tissue sediment, adding lysate, incubating and eddying at intervals, and centrifugally collecting supernate, namely the total protein of the rumen epithelial tissue of the milk cow. For the total protein of the rumen epithelial tissue of the milk cow, extracted by the method, a total protein mixture can be separated by a two-dimensional gel electrophoresis technology according to an isoelectric point and molecular weight of the protein, the relative abundance of the protein is acquired, the isoelectric point, the molecular weight and a relative transcript level of each protein can be clearly and visually shown in a gel atlas, and the method has the advantages of more obvious integrity and visualization in comparison with the prior arts such as a high efficiency liquid chromatography separation method, an enzyme-linked immunosorbent assay and immunoblotting.

The present invention provides a corn active peptide additive for cell culture medium, wherein in the corn active peptide additive, oligopeptides with molecular weight of lower than 1000 Dalton account for equal to or more than 90 wt % of total proteins, and the oligopeptides at least comprise one or more of AP, SAP, PAL, VNAP, PSSQ, and TQPGPQ. The corn active peptide additive of the present invention can be compounded with various basic culture mediums for serum-free culture of various animal cells, which not only substantially lowers the cost for cell culturing and reduces pollution and other problems caused by an animal derived component, but also can promote cell proliferation, improve cell viability and enhance expression of cell products.

The invention discloses method for detecting and evaluating quality of peanuts suitable for soluble protein processing. A method for detecting the quality of the peanuts suitable for the soluble protein processing comprises the following steps of: detecting the following indexes of a peanut sample, such as crude fat content, total protein content, total sugar content, cystine content, arginine content, content of conarrachin I, mass percentage content of a subunit with molecular weight of 37.5kDa based on total protein, the mass percentage content of the subunit with the molecular weight of 23.5kDa based on the total protein, the mass percentage content of the subunit with the molecular weight of 15.5kDa based on the total protein, a protein extraction ratio and a kernel ratio; and substituting measured values into a formula (1) so as to obtain dissolubility of the peanut sample. The invention also provides a method for evaluating the quality of the peanuts suitable for the soluble protein processing, namely, detecting the dissolubility of the peanut sample to be detected according to the method, and classifying the peanut sample to be detected according to standards of the following 1) to 3): 1) if a calculated value of the dissolubility is not less than 86, the peanut sample is suitable for the soluble protein processing; 2) if the calculated value of the dissolubility ranges from 68-86, the peanut sample is substantially suitable for the soluble protein processing; and 3) if the calculated value of the dissolubility is not more than 86, the peanut sample is not suitable for the soluble protein processing.

The invention relates to a process for producing staphylococcus aureus protein A by utilizing recombinant pichia pastoris. According to the production process, plasmid pPIC9K is utilized for integrating genes in a protein A antibody-binding fraction into a genome of the pichia pastoris GS115, and screening, expression and optimization of purification conditions are performed to get the active excretion protein A. The invention provides preparation and screening of a recombinant strain, as well as a culture and fermentation method by utilizing the strain to express the protein A, and a purification method of the expressed protein A. The strain is firstly cultured in a BMGY (buffered glycerol-complex medium) culture medium for about 17h and then expressed, screened and optimized in a culture medium BMMY (buffered methanol-complex medium) with the pH of 2.6-5.6 and the methanol content of 0.5%-2.0%. Then the expression of the recombinant protein A is further optimized in a fermentation tank, the expression level of the final protein A can achieve 8-10g per liter of bacterial liquid, accounting for about 80% of the secreted total protein. The high-purity protein A can be obtained by filtering through a desalting column and purifying by ion exchange chromatography, and the yield is about 80%. The process creates favorable conditions for low-cost and large-scale production of the high-purity protein A.

The invention provides a purification method for split influenza virus vaccine. An influenza virus strain is inoculated onto a chick embryo and cultured to obtain a virus solution; the virus solution is sequentially subjected to inactivation and ultrafiltration concentration to obtain an ultrafiltrate; the obtained ultrafiltrate is subjected to cane sugar density gradient centrifugation by using a KII continuous flow centrifuge to obtain an ultra centrifugal solution; the ultra centrifugal solution is subjected to ultrafiltration dialysis for cane sugar removal and molecular sieve gel chromatography to obtain a virus purification solution; the obtained virus purification solution is subjected to virus split by using a split agent TritonX-100 and sodium deoxycholate; after the split is over, the split agent is removed via ultrafiltration dialysis; impure protein is centrifugally removed from the obtained split solution; supernatant is collected, filtered and sterilized so as to obtain the purified influenza virus vaccine primary solution. The purification method is simple and convenient to operate, high in centrifugation capacity and suitable for large-scale production; by adopting a dual-split agent and a centrifugal process, the finished product is greatly improved in activity (hemagglutinin content / protein total content), and approaches the activity ratio of subunit vaccine, as a result, the high-grade split influenza virus vaccine product is obtained.

A method of determining and evaluating quality of peanut raw material suitable for processing protein. The method includes the following step: determining fruit shape score, total protein content, leucine content, arginine content, conarachin I content and the mass percentage of the subunit with molecular weight of 23.5 kDa to total protein in the peanut sample to be tested; putting the determined values into formula (1) to obtain the protein powder quality of the peanut sample. The disclosure reduces the analysis step. The disclosure establishes the model of evaluating raw material quality for peanut protein processing, and the peanut protein powder quality can be determined by 6 peanut quality characteristics. The determination of indexes in the model can be predicted by the near infrared analyzer. Through the near infrared analysis of peanut kernel, the indexes in the model can be simultaneously predicted without any damage to the peanut kernel.

The invention discloses an industrial production method of preparing corn peptide powder by means of bionic enzymatic hydrolysis from corn proteins. The method is characterized by comprising the step of performing bionic enzymatic hydrolysis, high-speed centrifugation, ultrafiltration, electrodialysis, concentration and drying to obtain the corn peptide powder by taking the corn proteins as a raw material. The total protein content of the corn peptide powder is 90% above, the content of small molecule peptide which can be dissolved by trichloroacetic acid is 85% above, and the corn peptide powder has the advantages of meeting the selecting mechanism of human absorption, being free of toxic and side effects and low in cost and the like, and can be applied to food, health food and drugs.

The invention relates to a rabies and tetanus double valence human normal immunoglobulin, the rabies valence of antibody is not less than 100IU / mL, the tetanus valence of antibody is not less than 60IU / mL, the content of protein is not more than 175g / L, the immunoglobulin purity is not lower than 92% of the total protein content. The preparation method of source plasma of the protein is to select plasma supplier to be inoculated with rabies vaccine and then with adsorbed tetanus vaccine at least 1-5 days later to obtain the source plasma with rabies valence of antibody being not less than 6IU / mL and the tetanus valence of antibody being not less than 5IU / mL and ensure that the rabies valence of antibody of the source plasma is not less than 10IU / m and the tetanus valence of antibody is not less than 8IU / mL after mixture. The immunoglobulin of the invention can be used to immunize patients suffering exposed wounds caused by biting and scratching of a mad dog or other mad animals.

The invention discloses a method for measuring and evaluating quality of a peanut raw material for protein processing. The method for measuring comprises measuring a fruit shape score, total protein content, leucine content, arginine content, conarachin I content and mass percentage content of subunit having molecular weight of 23.5 kDa in total proteins of a peanut sample to be measured, substituting the above measured values into the formula (1) and acquiring protein powder quality of the peanut sample to be measured. The method reduces analysis steps and is suitable for enterprise application. A peanut protein processing raw material quality evaluation model is built. Through six peanut quality characteristics, the peanut protein powder quality is determined. A near-infrared analyzer can conveniently and fast forecast indexes such as amino acids in the model. Through near-infrared analyzing detection of peanut kernels, all indexes in the forecasting model can be acquired simultaneously and the damage to peanut kernels is avoided. The method is convenient and fast.

The present invention discloses a method for producing protein feed by utilizing fermentation of rice dregs. Said method includes the following steps: adding bulking agent into the rice dregs whose water content is 55-60% according to the ratio of 1:0.05-0.1, adding waste water obtained by producing monosodium glutamate according to the ratio of 1:0.08-0.15 and adding solid yeast according to the ratio of 1:0.08-0.15, uniformly stirring them for 8-15 sec. and mixing them and making fermentation for 22-26 hr. and drying so as to can obtain the invented product. The protein total content of said protein feed can be raised by 15%-21%.

In certain embodiments, the invention provides methods for treating cancer, comprising: (a) obtaining a specimen of cancer tissue and normal tissue from a patient; (b) extracting total protein and RNA from the cancer tissue and normal tissue; (c) obtaining a protein expression profile of the cancer tissue and normal tissue; (d) identifying over-expressed proteins in the cancer tissue; (e) comparing the protein expression profile to a gene expression profile; (f) identifying at least one prioritized protein target by assessing connectivity of each said over-expressed protein to other cancer-related or stimulatory proteins; (g) designing a first RNA interference expression cassette to modulate the expression of at least one gene encoding the prioritized target protein; (h): designing a first RNA interference expression cassette to modulate the expression of at least one gene encoding a protein of higher priority in the signaling pathway in which the first protein is a component; (i) incorporating the first cassette into a first delivery vehicle; (j) providing a patient with an effective amount of the first delivery vehicle; (k) extracting total protein and RNA from the treated cancer tissue; (l) identifying over-expressed proteins in the treated cancer tissue; (m) designing a second RNA interference expression cassette to modulate the expression of a second prioritized protein in the treated tissue; (n) incorporating the second cassette into a second delivery vehicle; (o) providing the previously treated patient with an effective amount of the second delivery vehicle; (p) identifying a novel protein signal following prior treatment with protein specific knockdown; (q) identifying a gene mutation provided by gene sequencing / microarray on assessment of other protein signals; and (r) identifying of a novel protein signal as a result of determination of the gene mutation and assessment of other protein signals to, directly or indirectly, modify the expression (i.e., production) of such proteins.

There is provided protein food products including Vicia faba and pea protein, wherein the total protein content is in the range of from 45 weight % to 55 weight % and the Vicia faba:pea protein ratio is in the range of from 1.1 to 1.8. There is also provided a process for making the disclosed protein food products.

The invention provides a method for producing yeast cells by fermenting a protein and impurity removed soybean liquid. A liquid left after separating soybean proteins from degreased soybeans and centrifuging for removing impurities is called as the protein and impurity removed soybean liquid. The protein and impurity removed soybean liquid also contains large amounts of low-molecular-weight proteins, carbohydrates, peptides and the like, and the method for producing the yeast cells by fermenting the protein and impurity removed soybean liquid allows the total protein yield of a single yeast to be 2.42g / L, the COD to be obviously reduced, the COD removal rate to reach 40%, and substantially solves resource waste and environmental pollution problems.