Analysis method for neuron injury related differentially expressed protein

A technology for differentially expressed proteins and neuron damage, which can be used in the analysis of materials, material analysis by electromagnetic means, material separation, etc., and can solve problems such as lack of drug treatment mechanism

Active Publication Date: 2016-04-06
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Abstract
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Problems solved by technology

In the research on the mechanism of Yangxue Qingnao Granules in the treatment of chronic cerebral insufficiency, there is a lack of research on the mechanism of drug treatment with protein expression as an indicator.

Method used

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  • Analysis method for neuron injury related differentially expressed protein
  • Analysis method for neuron injury related differentially expressed protein
  • Analysis method for neuron injury related differentially expressed protein

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Embodiment Construction

[0133] The present invention is further illustrated by the following examples, but not as a limitation of the present invention.

[0134] Instruments and reagents

[0135] The ultra-speed low-temperature centrifuge is a product of Beckman; high-performance liquid chromatography: model: RIGOL3220 (Beijing Puyuan Jingdian); chromatographic column: C18 reversed-phase column (Agela, C18 chromatographic column, 250 × 4.6mmi.d., packing particles Diameter: 5 μm); Tianjin Bona Aegel; Eksigent liquid phase-ABSCIEX TripleTOF5600 mass spectrometer and tandem mass spectrometry data analysis using ProteinPilot4.2 software are all products of ABI Company.

[0136] iTRAQ kit: iTRAQReagentMulti-PlexKit (AppliedBiosystems); Protein concentration detection kit (Bradford company); Tris (BBI company); NaF (Fluka company); Ethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) was purchased from Sigma; Leupeptin and Aprotinin were purchased from Shanghai Bioengineering Company. Acetone and ammoni...

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Abstract

The present invention relates to an analysis method for a neuron injury related differentially expressed protein. The method comprises: 1, constructing a zebrafish brain and central nerve injury model, and grouping; 2, carrying out drug treatment on the drug group; 3, extracting the total protein in each group; 4, loading the sample onto a mass spectrometer, and analyzing; and 5, carrying out bioinformatics analysis.

Description

Technical field: [0001] The present invention relates to the use of a protein analysis method in screening drugs, in particular to an analysis method for screening a differentially expressed protein related to neuron damage for the treatment of chronic cerebral insufficiency Background technique: [0002] Yangxue Qingnao Granule is a new traditional Chinese medicine independently developed by Tasly for the treatment of chronic cerebral ischemia caused by blood deficiency and liver hyperactivity. Mainly by improving meningeal microcirculation, increasing cerebral blood flow, significantly reducing the symptoms of neurological deficits caused by cerebral ischemia, significantly inhibiting nerve cell apoptosis and synaptic structural changes, and playing a role in the prevention and treatment of pathological changes in chronic cerebral ischemia . Neuronal damage caused by ischemia-reperfusion injury is one of the main causes of chronic cerebral insufficiency, and the pathogene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N27/62
Inventor 佟玲李晓稳李东翔陈楠孙万阳周水平朱永宏
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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