27 results about "Monocotyledon" patented technology

The invention belongs to the field of gene engineering, and relates to a plant genome directional editing tool based on transposon-coded nuclease. The invention aims to solve the technical problem that the application range of a plant genome editing tool based on CRISPR-Cas9 and Cas12a is limited to a great extent due to the fact that CRISPR-Cas9 and Cas12a are large in protein size and difficult to deliver. The invention provides transposon nuclease suitable for plant genome editing. The transposon nuclease is IsDge10, IsAam1 or enIscB subjected to codon preference optimization; and a gene editing system containing the three transposon nuclease is constructed. The gene editing system can be suitable for editing a coding region, a non-coding region and the like; the method can be widely applied to monocotyledonous plants, dicotyledonous plants, gymnosperm and the like.

Provided are a prime editing system-based method for achieving inversion editing of a large genomic fragment and a composition used. By means of the efficient multi-gene prime editing protein construct ePPEplus in plants, the inversion of large fragments in plants is achieved by means of designing paired pegRNAs (pegL and pegR), and then by means of processes such as reverse transcription, DNA double-stranded annealing, DNA strand synthesis, and repair. The pegL comprises RTT-L, and the pegR comprises RTT-R. The nucleotide sequence of RTT-L is identical to that of strand A at the right terminus of a double-stranded DNA to be inverted. The nucleotide sequence of RTT-R is identical to that of strand B at the left terminus of said DNA. By means of testing in the monocotyledonous plant wheat and dicotyledonous plants Nicotiana benthamiana and tomato, it is indicated that the method is an effective method for mediating precise inversion of large-fragment DNA in plants.

The invention belongs to the technical field of plant biology, and particularly relates to a protein coded by a larch LarGRF1 gene and application of the protein to plant growth promotion. The invention aims to provide a new choice for plant genetic breeding by exploring growth regulatory factors (GRFs) of larch. The technical scheme of the invention is that the protein coded by the larch LarGRF1 gene has an amino acid sequence as shown in SEQ ID No. 2. Through overexpression of the MarGRF1 gene in dicotyledonous plants and monocotyledonous plants, it is found that an overexpression vector can promote proliferation of plant cells, especially proliferation of calluses and accelerate regeneration and growth.

The invention belongs to the technical field of gene engineering, and discloses application of a Camellia oleifera CoSPX-MFS3 gene in improvement of phosphorus absorption of plants, and the nucleotide sequence of the Camellia oleifera CoSPX-MFS3 gene is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the Camellia oleifera CoSPX-MFS3 gene. The plant is any one of dicotyledonous plants or monocotyledonous plants. The dicotyledonous plant is any one of arabidopsis thaliana, tobacco or tomato; the monocotyledonous plant is any one of rice, wheat or corn. The low-phosphorus stress can induce the expression of the gene, and the abundance in roots and fruits is the highest. The CoSPX-MFS3 is constructed to an overexpression vector pBI121, and the overexpression vector pBI121 is converted into the silver gray poplar, so that a positive strain is obtained. The invention discloses the application of the Camellia oleifera CoSPX-MFS3 gene in improvement of phosphorus absorption of the plant for the first time, and experiments carried out in the Populus arvensis show that the Populus arvensis plant over-expressed with the CoSPX-MFS3 gene can significantly improve the phosphorus absorption capacity of the plant.

The invention provides a sclerotinia sclerotiorum mutant strain SEEN10 and application of the sclerotinia sclerotiorum mutant strain SEEN10 in monocotyledon yield increase, and belongs to the technical field of microorganism application. The invention provides a Sclerotinia sclerotiorum mutant strain SEEN10, wherein a gene knocked out of the Sclerotinia sclerotiorum mutant strain SEEN10 is a xylan hydrolase gene SsXyl2 (SS1G03618), and the gene knocked out of the Sclerotinia sclerotiorum mutant strain SEEN10 is a xylan hydrolase gene SsXyl2 (SS1G03618). When the mycelium suspension of the strain SEEN10 is applied to seed dressing treatment of wheat seeds, compared with a wild sclerotinia sclerotiorum strain, the yield increase of monocotyledons can be more remarkably promoted, and the pathogenicity of the strain SEEN10 to oilseed rape is greatly reduced. The growth promoting advantage of the strain SEEN10 provides a technical path for green agriculture.

The invention relates to an organic herbicidal composition based on extracts of waste plant matter from the leaves of plants of the Asparagales order, with added amino acids that inhibit the enzyme responsible for glutamine synthesis, which is effective in controlling and eradicating dicotyledon weeds (broadleaf) and monocotyledon weeds (grasses).

The application discloses a Gliocladium catenatum PE-1, which is classified as Gliocladium catenatum Clonostachys rosea f. catenulata Clonostachys rosea f , is preserved in the China General Microbiological Culture Collection Center on February 2, 2024, and has a preservation number of CGMCC NO. 41164. The application also discloses a bacterial agent prepared from the Gliocladium catenatum PE-1. The application further discloses applications of the Gliocladium catenatum PE-1 and the bacterial agent in preventing and treating plant diseases. The Gliocladium catenatum PE-1 screened in the application can widely induce the burst of active oxygen of a plant immune marker in monocotyledonous plants and dicotyledonous plants, and can effectively prevent and treat various plant diseases, such as soybean phytophthora root rot, rice rice blast, wheat powdery mildew and wheat scab, and provides a new strain resource and technical support for biological control of plant diseases.

The invention discloses a strain of pyrethrum delavayi PE-1, the classification name of the pyrethrum delavayi PE-1 is pyrethrum delavayi, the pyrethrum delavayi PE-1 is pyrethrum delavayi, the pyrethrum delavayi is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is February 2, 2024, and the preservation number is CGMCC NO.41164. The pyrethrum delavayi has the advantages that the pyrethrum delavayi can be used for preparing the pyrethrum delavayi; the invention also discloses a microbial inoculum prepared from the strain. The invention further discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis to prevention and treatment of plant diseases. The fermentation liquor of the screened euphorbia emerald PE-1 can widely induce the burst of reactive oxygen of plant immune markers in monocotyledonous plants and dicotyledonous plants, and can effectively prevent and treat various plant diseases such as phytophthora sojae root rot, rice blast, wheat powdery mildew, wheat scab and the like; new strain resources and technical support are provided for biological control of plant diseases.

The invention discloses a heat-resistant transgenic rice comprehensive cultivation method oriented to an extreme high-temperature environment, and relates to the technical field of agricultural biology. A core heat-resistant gene is screened from an extreme high-temperature biological resource, a transgenic expression vector is constructed by combining a monocotyledonous plant specific regulation element, abnormal cells are eliminated through detection such as imprinting hybridization on a plant cell level and multi-level identification, efficient and directional expression of a target gene in rice is ensured, and the gene expression efficiency is improved. Based on heat resistance and agronomic traits, differentiated high-temperature stress parameters are set according to the key growth stage of rice in a simulated extreme high-temperature environment, the survival rate and photosynthetic efficiency of plants at high temperature are guaranteed, and meanwhile it is guaranteed that the agronomic traits are not sacrificed through yield detection; the cultivated strain is suitable for planting in different high-temperature areas, reference is provided for forest breeding and seedling raising industries, and agronomic traits are guaranteed while heat resistance is improved.

The application provides a Lemna australis endogenous strong promoter, a recombinant vector containing the Lemna australis endogenous strong promoter, and application of the Lemna australis endogenous strong promoter and the recombinant vector in starting expression of a target gene in monocotyledonous plants and dicotyledonous plants. The Lemna australis endogenous strong promoter provided by the application has high activity in monocotyledonous plants and dicotyledonous plants, can maintain high activity under conditions of different concentrations of screening agents, and basically does not affect expression of adjacent genes under conditions of different concentrations of screening agents. The application can solve problems of the 35S promoter widely used in the prior art, such as difficulty in further improving activity, low activity in monocotyledonous plants, and influence on expression of adjacent genes.

The invention relates to application of Col05, Col06 or Col29 protein, the Col05, Col06 or Col29 protein is used for inhibiting or killing insects, the Col05, Col06 or Col29 protein respectively has an amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5, dicotyledonous plant tobacco is selected as a first host, monocotyledonous plant corn is selected as a second host, the Col05, Col06 or Col29 protein is subjected to plant codon optimization, and the Col05, Col06 or Col29 protein is obtained. And respectively obtaining nucleotide sequences as shown in SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 6. Compared with the prior art, the insect inhibition protein Col05, Col06 or Col29 and the application of the coding gene have the advantages that a more effective insect inhibition method, particularly a prevention and control method for diplodia juncoides, is provided, the use of insecticides can be effectively reduced, and the ecological risk can be remarkably reduced. The invention provides a brand-new inhibition method for the diplodia juncosa, the existing genes for resisting the diplodia juncosa are mainly RNAi, and the method provided by the invention is completely different from a method for resisting insects by using proteins in the aspect of insect resistance mechanism.

Monocot plants heterozygous for centromeric histone 3 (CenH3) and optionally expressing gene editing constructs, for use in inducing haploids of a monocot target plant and optionally pass-through gene editing are provided. The monocot haploid inducer plants are typically composed of diploid plant cells having only one allele encoding a functional CENH3 protein. The diploid plant cells can also include, for example, one CenH3 allele encoding non-functional CENH3 protein. In some embodiments, the allele encoding non-functional CENH3 protein is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The monocot haploid inducer plant can also include gene editing machinery, such as a site-directed nuclease and optionally a guide RNA stably expressed by cells of the monocot plant. Methods of inducing formation of a target haploid monocot plant while optionally simultaneously modifying the target monocot plant's genome are also provided.

Disclosed is a gene for boosting genetic transformation efficiency of wheat and use thereof, which belongs to the field of plant genetic engineering technology. The use of a TaHRF1 gene and an encoded protein thereof can promote integration of an exogenous nucleic acid molecule into a genome of a plant of interest, and boost efficiency of the integration of the nucleic acid molecule, and the transformed plant grows and develops normally. The plant of interest includes, but is not limited to, monocotyledonous plants such as wheat. The present disclosure provides a new and important genetic resource for overcoming the genotype dependence in genetic transformation of wheat.

The invention belongs to the field of plant genetic engineering, and particularly relates to a rice panicle development regulation gene PD5, a protein coded by the gene PD5 and application of the gene PD5. The invention discloses a rice panicle development regulation gene PD5, the nucleotide sequence of the gene PD5 is shown as Seq ID No: 1, and the cDNA nucleotide sequence of the gene PD5 is shown as Seq ID No: 2. The invention also provides application of the gene PD5. The PD5 is knocked out to promote the increase of the number of secondary branches and the number of grains per spike of monocotyledonous plants (such as rice) and increase of per unit area.

The disclosure provides novel methods for improving the regeneration throughput and / or plugging frequency of monocot embryo explants by flotation and selective collection of viable and regenerable explants. Genetic modification or transformation of monocot plants may include preparation of seed excised embryo explants, rehydration, inoculation and co-culture, bud induction, extended bud induction, and regeneration of genetically modified plants or plant parts. To improve regeneration and plugging frequency, cultured monocot seed excised embryo explants following the bud induction or extended bud induction step are placed in a flotation medium to separate the explants into top and bottom layers or fractions, such that the top fraction can be selectively collected and advanced to regeneration media. The top fraction is shown to have a higher or similar plugging frequency than the bottom fraction and thus the flotation step reduces costs and improves transformation throughput by removing unproductive explants in the bottom fraction having a much lower regeneration and plugging frequency.

The utility model discloses a light combined arched farmland flying prevention isolation device, including the isolation cover of coverable setting above the plant, the both ends of isolation cover along width direction are detachably penetrated with support piece, the support piece has the elastic deformation ability, and the support piece after bending deformation can maintain isolation cover is round arch structure setting. The utility model provides technical scheme, sets above the monocotyledon or dicotyledon crop with a detachable isolation device, combines good and fixes above the plant before spraying pesticide or herbicide, can avoid pesticide or herbicide mist drop to scatter to above the plant, can play the protection effect to monocotyledon or dicotyledon crop, and whole can be freely combined according to the size of crop area, and the more wide application range is suitable.

Disclosed is a gene for boosting genetic transformation efficiency of wheat and use thereof, which belongs to the field of plant genetic engineering technology. The use of a TaHRF1 gene and an encoded protein thereof can promote integration of an exogenous nucleic acid molecule into a genome of a plant of interest, and boost efficiency of the integration of the nucleic acid molecule, and the transformed plant grows and develops normally. The plant of interest includes, but is not limited to, monocotyledonous plants such as wheat. The present disclosure provides a new and important genetic resource for overcoming the genotype dependence in genetic transformation of wheat.

The application provides application of a bacterial protein bGM to improve plant disease resistance, and relates to the technical field of biological gene engineering. The bGM protein with the amino acid sequence shown in SEQ ID No. 3 is used to improve plant disease resistance. The bGM protein in bacteria is used to improve plant disease resistance for the first time, and the available disease resistance gene resources can be greatly expanded. The bacterial bGM protein in the application can improve disease resistance in both dicotyledonous plants tobacco and monocotyledonous plants rice, while traditional disease resistance genes are often limited to specific species to play a function. The bGM protein in the application is a protein derived from bacteria, and the research on disease resistance in bacteria is relatively deep, and there are a large number of disease resistance gene resources, which are directly applied to plant disease resistance breeding, and time and labor are saved.

The invention discloses an application of a sugarcane ScWRKY24G gene in improving bacterial blight disease resistance of plants, the ScWRKY24G gene is amplified from a sugarcane variety cinnamyl sugar 42, and the nucleotide sequence of the ScWRKY24G gene is as shown in SEQ ID NO.1. The invention also discloses an application of the sugarcane ScWRKY24G gene in improving bacterial blight disease resistance of plants. According to the invention, an overexpression vector p35S-ScWRKY24G-Flag of ScWRKY24G is constructed, Nipponbare rice (Nip, WT) is transformed by using agrobacterium, an overexpression plant is obtained, and an analysis result shows that the overexpression ScWRKY24G gene can improve the resistance of rice to bacterial blight. According to the invention, bacterial leaf blight resistance gene resources are enriched, and the ScWRKY24G gene is applied to breeding of monocotyledonous plants for disease resistance, so that the cultivation of new varieties or germplasm materials of the bacterial leaf blight resistant monocotyledonous plants is facilitated.

The present application relates to a kind of monocotyledon green wisteria rapid propagation method, using the characteristics of green wisteria specific tissue cell totipotency, under open condition, using liquid medium to induce green wisteria specific site of in vitro leaf split differentiation, produce regenerative root and regenerative bud, finally form plant.The present application aims at the rapid propagation method of green wisteria leaf, without sterile environment, convenient to take material, easy to operate, low in cost, short in period, high in survival rate, with the advantages of high efficiency, economy, easy to scale, factory, etc., in green wisteria rapid seedling, specific material preservation and propagation has greater application value.

Disclosed is a method for boosting genetic transformation efficiency of wheat using an Agrobacterium-mediated method, which belongs to the field of plant genetic engineering technology. A TaHRF2 gene and an encoded protein thereof used in the present disclosure can promote introduction of a nucleic acid molecule into a plant of interest and boost genetic transformation efficiency of the introduction of the nucleic acid molecule into the plant of interest. The plant of interest includes, but is not limited to, monocotyledonous plants such as wheat. By introducing a TaHRF2 gene high-expression vector into an Agrobacterium strain, and infecting a wheat explant using an Agrobacterium-mediated method, genetic transformation capacities of backbone parents such as Beijing 8 and Lumai 1 are enhanced, providing important information and fundamental technical support for the establishment of an efficient genetic transformation system for major wheat cultivars with difficulty in regeneration.

The application discloses application of a rice OsRbohI gene in promoting growth of rice and relates to the technical field of bioengineering. The application can improve plant height and root length and increase grain size by cloning the rice OsRbohI gene, performing genetic transformation in rice and overexpressing the rice OsRbohI gene; loss of the function of the OsRbohI gene can cause dwarfing of the plant, shortening of the root and reduction in the grain size. Changing the plant type and grain type of rice by means of genetic engineering technology has important research significance for improving crop production. Based on the close relationship between rice and other monocotyledonous plants, the rice OsRbohI gene can regulate growth and development of rice, especially the plant height, root development and grain size. The research result can also be applied to other monocotyledonous plants to cause change in the plant type and grain type.

This invention provides a method for establishing a rapid propagation system of *Iris tectorum* through tissue culture using autumn dormant buds. The method uses autumn dormant buds of *Iris tectorum* as explants. Aseptic buds are obtained by removing the base and excess above-ground parts, fixing, peeling, and clamping. The buds are then longitudinally cut to increase the propagation coefficient, inducing clustered lateral buds, and then inducing rooting to form aseptic mature plants. The aseptic rate after 7 days is 57.12%, and the fungal rate is 0.15%. The aseptic rate after 20 days of the first subculture is 84.66%, and the rooting rate after 50 days is 63.33%. This method eliminates the need for callus induction and differentiation stages, significantly shortening and simplifying the time and procedure for obtaining aseptic *Iris tectorum* seedlings. The method is easy to control, low in cost, and highly efficient. It provides a new approach for further subculture or callus redifferentiation to establish a highly efficient rapid propagation system for monocotyledonous plants, and provides a solid foundation for the breeding, asexual reproduction, polyploid breeding, and even genetic engineering breeding research of *Iris tectorum* original species and varieties.

The application discloses corn ZmDLR9 Gene in regulating plant lateral root development belongs to the technical field of plant genetic engineering and crop genetic improvement. ZmDLR9 The gene can not only play a role in promoting lateral root development in corn, but also can replace the function of a homologous gene in dicotyledonous plants Arabidopsis thaliana. Through a back-up experiment, it is proved that ZmDLR9 The gene can save the lateral root development defect of Arabidopsis thaliana homologous protein APUM24 after loss of function, which indicates that the function of the gene in promoting lateral root development has cross-species conservation, and further proves that ZmDLR9 The gene promotes the development of plant lateral roots, and is not a specific regulation mechanism of corn. Therefore, ZmDLR9 The gene regulates the development of plant lateral roots in monocotyledons and dicotyledons, and has important agricultural production application value for cultivating new varieties with more developed lateral root systems, higher nutrient and water absorption efficiency and stronger stress resistance (such as drought resistance and tolerance to barrenness).

The invention relates to the technical field of agricultural biology, in particular to application of carbamylglutamic acid to improvement of pregnancy characteristics of sheep. The application of the faecium carbamylglutamic acid in improving the pregnancy characteristics of the sheep comprises the following steps: improving uterus and fetus development, including increasing the average weight of uterus, the number of fetuses, the weight of nests and fetuses, the number of corpus luteum and the corpus luteum ratio of the fetuses, increasing the number of uterine glands and reducing the thickness of uterine mucosa; and improving the development of cotyledons, including increasing the total weight of the cotyledons and the average weight of monocotyledons.

The application discloses a kind of spirocyclic amino acid skeleton compounds, it is compound shown as formula I with structural general formula, there are 29 kinds of compounds.The application also discloses the preparation method of spirocyclic amino acid skeleton compound.The application also discloses the application of spirocyclic amino acid skeleton compound in as or preparation herbicide, in the application in weed control or preparation in weed control preparation, the control object or weed of the herbicide is selected from the group consisting of barnyard grass, dogtail grass, Chinese alpine paper, crabgrass and other monocotyledonous plants, and purslane, amaranthus retroflexus, goosefoot, solanum nigrum, abutilon theophrasti and other dicotyledonous plants.Spirocyclic amino acid skeleton compound of the application is obtained after molecular modification to microsphaeropsis ochracea ketonic acid, and experimental research shows that the weed control effect is good, especially compound C3, compound C22, compound C23, the growth of crop has no influence basically.Spirocyclic amino acid skeleton compound of the application is suitable for industrial production, and has great application potential.

Provided herein are polynucleotides encoding recombinant plant transcription factors, recombinant plant transcription factors, and methods of producing recombinant monocotyledonous plants using recombinant plant transcription factors.