293 results about "Monocotyledon" patented technology

A process for integrating a DNA fragment into the genome of a cell of a monocotyledonous plant, the process comprising the steps of: 1) incubating, prior to contacting with the DNA fragment, a culture of untransformed monocotyledonous plant cells on a medium comprising a plant phenolic compound, for a period of time sufficient to stimulate cell division and enhance competence for integration of foreign DNA; and 2) contacting the untransformed cells with the DNA fragment under conditions in which the DNA fragment is taken up by the untransformed cells and is stably integrated in the genome of the untransformed cells, to generate transformed cells.

The invention relates to a promoter, in particular to a promoter for monocotyledons such as rice, and a preparation method and application thereof. The promoter of the invention has a nucleotide sequence shown in SEQ ID NO: 1, or has a variant which has the functions of the promoter and is selected from the following sequences: 1) a nucleotide sequence hybridized with the nucleotide sequence shown in SEQ ID NO: 1 under the condition of advanced tightness; 2) a nucleotide sequence carrying out substitution, loss, modification and addition of one or more basic groups on the nucleotide sequence shown in SEQ ID NO: 1; and 3) a nucleotide sequence having at least 90 percent of sequence identity with the nucleotide sequence shown in SEQ ID NO: 1. In addition, the invention also relates to a method for preparing the promoter and the application of the promoter in adjusting and controlling the target gene expression in the monocotyledons.

The invention discloses a plant gene knockout vector based on a CRISPR/Cas9 technology. The monocotyledon plant gene knockout vector is characterized in that the plant gene knockout vector is a monocotyledon plant CRISPR/Cas9 knockout vector pCambin1300-OsU3-Cas9, and contains the AarI insertion site. According to the present invention, with the CRISPR/Cas9 plant knockout double-component vector pCambin1300-OsU3-2x35s-Cas9 formed from a sgRNA-OsU3 structure sequence comprising the AarI insertion site and a 2x35s-Cas9-ter sequence, the purpose of the efficient and rapid construction of the monocotyledon plant gene knockout vector can be achieved through the one-step digestion linkage method; and the provided CRISPR/Cas9 knockout vector pCambin1300-OsU3-2x35s-Cas9 has characteristics of high targeting efficiency and extremely low undershoot efficiency, and can be used for plant target gene knockout or can be used for plant gene editing in a kit form.

The invention discloses a special expressing promoter of plant endosperm and appliance, which is characterized by the following: 1) possessing DNA sequence of sequence 1 in sequence table; 2) possessing 70% or above 70% homologous property with limited DNA sequence of sequence 1 in sequence table; possessing DNA sequence with same function; 3) possessing crossing nucleic acid sequence with limited NDA sequence of sequence 1 in sequence table under high strict condition. This promoter can start special expression of external source gene in plant endosperm, which is fit for any plant with endosperm such as monocotyledon or dicotyledon.

The invention relates to a gene deletion system for the security control of transgene monocotyledon, comprising two homodromous loxP and FRT fused specificity recognition sites loxP-FRT, wherein a section of multiple clone site sequence for inserting a target gene to be led in plant is contained between the two specificity recognition sites. The gene deletion system can completely delete foreign genes in a specific plant organ by a plant expression vector which is formed by leading a monocotyledon tissue-specific promoter sequence, and can achieve the deletion efficiency of 99.8 percent, thereby being used for preparing the safe transgene monocotyledon.

The invention relates to a method of enhancing the seed yield and promoting the growth of monocotyledonous or dicotyledonous plants by overexpression of TMT (tonoplast monosaccharide transporter) protein in osogenic or transgenic plant cells. The invention further concerns a transgenic plant having the property of an enhanced seed yield and increased growth as compared with the wild type, comprising a nucleotide sequence which codes for a TMT (tonoplast monosaccharide transporter) protein, as well as a regulatory nucleotide sequence, operably linked therewith, for the control of an increased gene expression of the TMT protein in the plant cells of the transgenic plant. It further relates to the use of the transgenic plant for the cultivation or production of cultivated plants or useful plants, or biomass, oils or proteins produced therefrom.

The invention provides novel methods and compositions for modulating gene function in plants. In particular, the invention provides methods and compositions that allow, for the first time, virus-induced gene silencing in rice. The invention is significant in that prior techniques were not available for rice and because of the major importance of rice to agriculture. The invention therefore provides techniques for the analysis of gene function in rice, as well as in other monocotyledonous species and dicotyledonous plant species.

The invention provides an insect-resistant gene Cry1Ab-Material. The synthesis of the gene is as follows: according to the amino acid sequence of the N-terminal of the original Bacillus thuringiensis (Bt) protein (Cry1Ab), the preferred codons of monocotyledon (corn) is used to perform human reformation and synthetize a new Cry1Ab DNA sequence; and the prokaryotic expression vector and plant expression vector are constructed, and transformation is performed in the host cells. Vitro tests prove that the transformed and synthetized Bt gene toxic protein has obvious insecticidal effect on Ostrinia nubilalis. The expression of the insect-resistant gene Cry1Ab-Ma in monocotyledon is stable and efficient, thus the gene can be used to produce insect-resistant transgenic plant.

The invention discloses a soybean zinc finger protein gene STF-2 which is a C2H2 type zinc finger protein gene related to drought and newly discovered in soybeans. Experiments show that: the STF-2 encoded protein can be located in the nucleus; after over-expression, the gene can obviously improve the drought resistance of the transgenic plants, and has a function of improving the comprehensive stress resistance of the plants; the gene disclosed by the invention comes from soybeans and has an optimized codon suitable for the dicotyledons such as soybeans and the like, and the genetic engineering receptor thereof is mainly suitable for the dicotyledons such as soybeans, tobaccos, cotton and the like; and moreover, the gene is also suitable for the monocotyledons such as rice, wheat, corn and the like.

Disclosed herein are media, systems and methods for achieving micropropagation of monocotyledonous plants.

The invention discloses an oidium heveae Steinmann in-vivo promoter WY51 and application thereof. The promoter WY51 has a nucleotide sequence as shown in SEQ ID NO: 1, or is provided with a variant with functions of the promoter. The invention further relates to a nucleic acid builder, a carrier, reconstitution cells, a transgenic plant, an explant and a callus which comprises the promoter. The promoter can be used for regulating and controlling expression of exogenous genes in dicotyledon and monocotyledon, and a brand-new tool and selection are provided for expression of genes of the transgenic plant.

The invention relates to a promoter, in particular to a promoter of a monocotyledon such as paddy rice, and a preparation method and application of the promoter. The promoter has a nucleotide sequence shown by SEQ ID NO:1, or has a variant which has a function of the promoter and is selected from the following sequences: 1) a nucleotide sequence in hybridization with the nucleotide sequence shown by the SEQ ID NO:1 under a high-grade stringent condition; 2) a nucleotide sequence for performing substitution, deletion and adding modification on one or more basic groups of the nucleotide sequence shown by the SEQ ID NO:1; and 3) a nucleotide sequence having at least 90 percent of sequence identity with the nucleotide sequence shown by the SEQ ID NO:1. The invention also relates to the preparation method of the promoter and the application of the promoter in adjusting the expression of a target gene in the monocotyledon and adjusting rice breeding.

This invention relates to a method for the introduction of genes encoding desirable traits into both monocotyledonous and dicotyledonous plants and to plants and parts thereof produced by growing plants using this method. The time required for the production of transgenic plants is significantly decreased, while the number of transgenic plants is significantly increased. These increases are not dependent upon the use of super-virulent Agrobacterium strains. The invention also relates to an improved technique for in vitro regeneration of mono- and di-cotyledonous plants in a suitable medium containing a novel growth regulator regime that promotes cell elongation in the production of numerous somatic embryos that are regenerable into fertile plants.

The invention provides a cold inducible expression promoter Poscold3 of a plant stem leaf and an application of the promoter. The invention further provides an expression box with the promoter, a plant expression vector, a host bacterium and a converter. Particularly, the invention obtains the promoter and uses the promoter in plant transgenetic engineering. The promoter provided by the invention can drive an exogenous gene to be specifically expressed in stems and leaves of a plant under a cold inducible condition. The promoter is suitable for monocotyledon cereal, and particularly can drive the exogenous gene to be expressed under induction in stems and leaves of rice plant, so that the promoter can be used for enhancing and improving the growth characteristic and cold resistance of rice, thereby cultivating an ideal rice variety.

The invention discloses a novel beta-1,6-glucanase, an encoding gene thereof, and applications of the encoding gene. The invention provides the beta-1,6-glucanase gene belonging to the glucoside hydrolase system, wherein the nucleotide sequence of the beta-1,6-glucanase gene is shown in SEQ ID NO.1, and the protein (amino acid) sequence of the encoded outer membrane glucoside hydrolase is shown in SEQ ID NO.2. The beta-1,6-glucanase can effectively prevent the infection of plant pathogenic fungi to plants. An engineered strain established by the gene realizes the prokaryotic expression of the beta-1,6-glucanase gene, and verifies the function of the glucoside hydrolase of the beta-1,6-glucanase gene, the result shows that GluM can effectively inhibit the germination of rice blast spores, and decompose the rice blast spores. After the beta-1,6-glucanase gene is transplanted into the dicotyledonous model plant arabidopsis thaliana and the monocotyledonous model plant rice, the result shows that the obtained transgenic plants have good anti-infection effects for grey mould and rice blast.

The invention was involved in gene engineering field. The method was about using RNA construction region of FCA gene to control blooming to modify crop economic characteristic. Encoded sequences of one or several whole construction region in proteins encoded by FCA whole gene were transferred into target plants. RRM2 construction region covered by rFCA-1 whole gene was amplified by RT-PCR method and was inserted into expression vector pB-1/520, then it was transferred into iaponica rice breed medium flower 11.T0 plant with modified gene and T1 (filial generation) showed obvious change on plant shape, leaf shape, size and weight of seeds. The method was applied in economic characteristic of both dicotyledon and monocotyledon.

The invention discloses application of paddy rice BG1 proteins and encoding genes of the paddy rice BG1 proteins to adjusting growth and development of plants and particularly provides application of proteins consisting of the amino acid sequences represented by Sequence 2 in a sequence table or the encoding genes of the proteins to adjusting and controlling growth and development of plants. Experimental results show that overexpression of the BG1 genes in wild Nipponbare causes increase of the sizes of paddy rice grains, the thousand seed weight, the single-plant yield and biomass, and imply the possible application potential of the BG1 genes in agricultural production. Meanwhile, overexpression of the BG1 genes in arabidopsis can enlarge organs (seeds and leaves), which shows that the BG1 genes probably have a common conservative mechanism in adjusting and controlling the sizes of grains (seeds) in monocotyledons and dicotyledons. The BG1 proteins and the encoding genes have a wide market and application prospect in the fields of crop genetic improvement and the like.