Method of enhancing the seed yield and promoting the growth of plants

A technology for promoting growth and monocotyledonous plants, applied in botany equipment and methods, chemical equipment and methods, biochemical equipment and methods, etc.

Inactive Publication Date: 2013-05-08
TECH UNIV KAISERSLAUTERN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No difference was detected for the disaccharide sucrose

Method used

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  • Method of enhancing the seed yield and promoting the growth of plants
  • Method of enhancing the seed yield and promoting the growth of plants
  • Method of enhancing the seed yield and promoting the growth of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] Cloning of plasmid constructs for overexpression of TMT protein (Attmt1)

[0037] The cDNA of the Arabidopsis TMT (tonoplast monosaccharide transporter) protein TMT1 (=Attmt-cDNA) was used for the expression of the sugar transporter. The hydrophilic loop region between putative transmembrane domains 6 and 7 was used to make overexpression constructs with modified Attmt-cDNA containing the c-myc motif. This is the construct pMUT3 (see figure 1 ).

[0038] The complete cDNA sequence was first cut from plasmid pMUT3 with restriction enzymes EcoRI and Clal, ligated into plasmid pHannibal and linearized with the same enzymes (pSR3). An expression cassette was formed in which the cauliflower mosaic virus 35S promoter was located upstream of the TMT1-cDNA and the OCS terminator was located downstream of the downstream terminal gene (polyadenylation signal of the octopine synthase gene). Plasmid pSR3 was further subjected to restriction digestion with the restriction enzyme ...

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Abstract

The invention relates to a method of enhancing the seed yield and promoting the growth of monocotyledonous or dicotyledonous plants by overexpression of TMT (tonoplast monosaccharide transporter) protein in osogenic or transgenic plant cells. The invention further concerns a transgenic plant having the property of an enhanced seed yield and increased growth as compared with the wild type, comprising a nucleotide sequence which codes for a TMT (tonoplast monosaccharide transporter) protein, as well as a regulatory nucleotide sequence, operably linked therewith, for the control of an increased gene expression of the TMT protein in the plant cells of the transgenic plant. It further relates to the use of the transgenic plant for the cultivation or production of cultivated plants or useful plants, or biomass, oils or proteins produced therefrom.

Description

technical field [0001] The present invention relates to a method for increasing seed yield and promoting the growth of monocotyledonous and dicotyledonous plants by overexpressing TMT (tonoplast monosaccharide transporter) protein or its homologue or its equivalent in isogenic or transgenic plant cells realized by analogs. The present invention also relates to a transgenic plant having improved seed yield and growth-promoting properties compared to the wild type, said transgenic plant comprising a nucleotide sequence encoding a TMT (tonoplast monosaccharide transporter) protein and operably linked thereto A regulatory nucleotide sequence for controlling increased gene expression of a TMT protein in a plant cell of a transgenic plant. The invention also relates to the use of said transgenic plants for cultivation and production of cultivated plants and useful plants or biomass, oil or protein produced therefrom. The present invention also relates to a gene construct, which in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/82A01H5/00
CPCC07K14/415C12N15/8261A01H5/00C12N15/11Y02A40/146
Inventor 埃克哈德·纽豪斯奥利弗·坦安特曼亚历山德拉·沃米特卡琳娜·温恩特
Owner TECH UNIV KAISERSLAUTERN
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