The invention discloses a method for synthesizing rebaudioside-A through high-density fermentation. The method comprises the steps that firstly, genes encoded with a cell wall synthetase system is knocked out, and fermentation synthesis of the rebaudioside-A is achieved while the permeability of a cell wall is improved; secondly, thalluses obtained through fermentation are applied to a whole-cellcatalysis reaction, and the high recovery ratio of the rebaudioside-A is achieved; in the high-density fermentation process, when the thalluses grow to the amount of (10-25) g/L (DCW), a culture medium is subjected to fed-batch in a mode of constant dissolved oxygen index feeding, and when cells grow to the stable phase, the density OD<600> of the thalluses reaches to 100-150; the thalluses grow to the stable phase, the culture medium is subjected to fed-batch in a mode of constant speed feeding, and the content of the rebaudioside-A in fermentation liquid after fermentation is completed is 13.7+/-1.6 g/L; and the thalluses collected in fermentation are subjected to buffer cleaning treatment by using potassium phosphate and then used for a 50 L whole-cell catalysis reaction, and the concentration of the rebaudioside-A in the system is 28.2+/-4.9 g/L. According to the method, the problem that the conversion rate of stevioside in cell fermentation is low is solved well, operation is easy, production is easy to enlarge, and the good rebaudioside-A preparing prospects are achieved.