8 results about "Fetal protein" patented technology

The invention belongs to the technical field of biomedical detection, and particularly discloses a nucleic acid aptamer for specifically recognizing serum alpha fetoprotein and application of the nucleic acid aptamer. The nucleotide sequence of the nucleic acid aptamer comprises a sequence as shown in SEQ ID No.1, or has more than 90% of homology with the sequence as shown in SEQ ID No.1, or one or more nucleotides are deleted or added in the sequence as shown in SEQ ID No.1. The nucleic acid aptamer can realize specific efficient recognition of AFP protein, also shows extremely high selectivity in a complex serum sample environment, eliminates non-specific adsorption interference of homologous protein and other serum interference protein, greatly reduces the false positive rate, and improves the accuracy and specificity of clinical detection. And the material constructed by directionally functionalizing the aptamer on the surface of the magnetic nano-carrier has rapid magnetic response performance, separation and enrichment of target protein can be realized within seconds, and the clinical diagnosis time is greatly shortened.

The invention provides a computer-implemented method and system for non-invasive and step-by-step prediction of cancer risks and a storage medium. The method comprises the following steps: (1) determining a cancer signal score based on the level of a biomarker in a blood sample of a subject; (2) comparing the cancer signal score with a preset threshold value, and determining a primary screening positive subject; (3) further performing cancer risk determination and cancer species prediction by adopting an NGS method on the basis of the primarily screened positive subjects; wherein the biomarker is selected from at least one of AFP (Alpha Fetal Protein), CA125, CA15-3, CA19-9, CA72-4, CEA (Carcino Embryonic Acid), CYFRA (Cytochrome Factor Receptor Amplification) 21-1, ProGRP (Proso-Growth Radical Polymerase Chain Reaction), SCCA (Sequence

The invention provides a living cell surface double-tumor marker in-situ detection method based on a closed bipolar electrode (cBPE) array and an electrochemiluminescence (ECL) technology. The living cell surface double-tumor marker in-situ detection method is used for synchronously quantifying carcino-embryonic antigen (CEA) and alpha-fetoprotein (AFP). According to the system, the high specific surface area, the ordered porous structure and the rich surface amino active sites of a covalent organic framework (COF) material are utilized, and the excellent conductivity and biocompatibility of gold nanoparticles (Au NPs) are combined, so that the bifunctional signal probe COF (at) Au (at) MBApt is constructed. The probe can simultaneously load a large number of signal molecule methylene blue (MB) and is coupled with a targeting aptamer (Apt), so that high-capacity labeling and specific recognition of a target marker are realized.

This invention discloses an ultrasensitive and rapid sensor for detecting alpha-fetoprotein (AFP), constructed based on a sandwich immunoassay structure and the electrochemiluminescence resonance energy transfer (ECL-RET) principle. The advantages of this invention compared to existing technologies are: high sensitivity and ultra-low detection limit: This invention innovatively uses MIL-NH2-RuMOF@Pt as an energy donor, whose MOF structure can load a large amount of Ru(bpy)3. 2+ The luminescent molecule provides a strong and stable initial ECL signal; ZnO@PDA acts as the acceptor, with its absorption spectrum highly overlapping with the donor emission spectrum, and PDA modification improves biocompatibility and coupling efficiency. The highly efficient RET pair formed by these two molecules, combined with the sandwich immunoamplification effect, achieves efficient signal quenching, resulting in a detection limit as low as 1.1 × 10⁻⁶. ‑ 14 mg·mL⁻¹; wide linear range; excellent specificity and accuracy; good stability and repeatability; broad application prospects.

The invention belongs to the technical field of assisted reproduction, and discloses an IVM serum-free culture solution based on fetuin. The IVM serum-free culture solution comprises the following components: an IVM serum-free culture basic solution, 0-10 mg / mL of non-typing fetuin, and the non-typing fetuin comprises fetuin-A (fetuin-A) and fetuin-B (fetuin-B), and the IVM serum-free culture basic solution comprises the IVM serum-free culture basic solution and the non-typing fetuin-A (fetuin-A) and the fetuin-B (fetuin-B), the IVM serum-free culture basic solution is prepared from 2.5 to 10 mM of sodium pyruvate, 5 mg / mL of human albumin, 10 ng / mL of epidermal growth factor (EGF), 0.075 IU / mL of follicle stimulating hormone (FSH), 0.075 IU / mL of human chorionic gonadotropin (hCG), an amino acid composition, vitamins and inorganic salt. Experiments show that by adopting the formula, the maturation rate of oocytes can reach 95.55% + / -0.51%, the natural fertilization rate can reach 90.68% + / -1.12%, the blastocyst rate can reach 85.02 + / -1.85%, the problems of cumulus expansion abnormity, zona pellucida sclerosis (ZP2 abnormal cutting), embryo morphology abnormity and the like in a serum-free system can be synchronously solved, the effect is superior to that of a single serum-B (10 [mu] g / mL) culture solution, a single serum-A (0.9 mg / mL) culture solution or a serum-containing culture solution, and serum pathogen risks and ICSI operation dependence are avoided.

The invention discloses a preparation method of a photoelectrochemical biological aptamer sensor for detecting alpha fetoprotein based on a binary doped composite material, and relates to a preparation method of a photoelectrochemical biological aptamer sensor for detecting alpha fetoprotein. The technical problems that an existing electrochemical biosensor for detecting alpha fetoprotein is low in sensitivity, narrow in detection range, low in detection speed and poor in stability are solved. The preparation method comprises the following steps: 1, preparing TiO2 NRA / FTO; (2) preparing a Sn-TiO2 NRA / FTO electrode; (3) preparing a BiVO4 / Sn-TiO2 NRA / FTO (fluorine-doped tin oxide) electrode; and 4, preparing the photoelectrochemical biological aptamer sensor. The linear detection range of alpha fetoprotein detected by the sensor is 0.001 ng / mL-500 ng / mL, the detection limit is as low as 0.75 ng / mL, and excellent gt is shown; the method has long-term stability in 850 seconds, low detection limit and short detection time, and can be used in the field of alpha fetoprotein detection.

The application discloses a hepatocellular carcinoma auxiliary screening method and system. The method comprises the following steps: inputting a liver occupying gray scale ultrasonic image of a to-be-screened object into a trained hepatocellular carcinoma (HCC) diagnosis model for screening to obtain an HCC screening result of the object; the HCC diagnosis model is obtained by training a neural network model by using training image samples generated by the liver occupying gray scale ultrasonic image; and the liver occupying gray scale ultrasonic image at least comprises a liver occupying gray scale ultrasonic image of which serum alpha-fetal protein (AFP) detection is negative and pathological diagnosis is hepatocellular carcinoma. The diagnosis model obtained by the technical scheme provided by the application can accurately identify patients with serum alpha-fetal protein negative and hepatocellular carcinoma, and has low cost, thereby providing a reference for medical staff to judge whether fine examination needs to be performed on patients with serum alpha-fetal protein negative and hepatocellular carcinoma risk.

The invention belongs to the technical field of biosensor preparation, and relates to a preparation method of a lactic acid and alpha fetoprotein detection biosensor. The preparation method comprises the following steps: adding single-layer graphene oxide into N, N-dimethylformamide, uniformly mixing, adding polyacrylonitrile, uniformly mixing, and adding dopamine hydrochloride to obtain a spinning solution; and after spinning with the spinning solution, drying in a vacuum drying oven, and then fixing on a gold electrode to obtain an electrode P. And uniformly mixing lactate oxidase and chitosan, dropwise adding the mixture to the surface of the electrode P, dropwise adding an antibody solution after drying, and performing secondary drying to obtain the sensing electrode. The nanofiber membrane with proper pore diameter and fiber diameter is regulated and controlled, is used for preparing a sensor for simultaneously detecting lactic acid and alpha fetoprotein, and has higher performance in detection of a real sample. The detection range of lactic acid and alpha fetoprotein is wide, the preparation process is simple, the cost is low, and the method can be used for large-scale production.