45 results about "Fetal protein" patented technology

The invention discloses a preparation method of an unlabelled photoelectrochemical alpha fetoprotein immunosensor, and belongs to the fields of novel nano functional materials and biosensors. On the basis of a titanium dioxide nano particle base, a trimetal alloy nano material in a dendrite nanorod form is prepared by a photoelectrochemical synthetic method, so that the prepared unlabelled photoelectrochemical alpha fetoprotein immunosensor for detecting alpha fetal protein is low in cost, high in sensitivity, good in specificity, fast in detection and simple to prepare.

The invention provides a hepatocellular carcinoma targeting gene expression element AP and applications thereof, and the gene expression element AP has a nucleotide sequence indicated in SEQ ID No.1. After the expression element AP is guided into Alpha-fetoprotein positive tumor cells, the artificial microRNA aiming at DNA polymerase can be expressed in the cell and the expression of the DNA polymerase in the cell can be effectively inhibited, the copy of the DNA in the cell is further affected; the cell cycle of is blocked finally, the multiplication of the AFP positive hepatoma cell is specially inhibited and the expression element AP can be applied to the preparation of the targeting gene curing medicine of AFP (alpha fetal protein) positive liver cancer.

The invention discloses an AFP and GM-CSF dual-gene co-expression recombinant vector, which is sequentially linked with an AFP gene, an IRES sequence and a GM-CSF gene in a vector transcription direction, or is sequentially linked with a GM-CSF gene, an IRES sequence and an AFP gene in a vector transcription direction, wherein nucleotide sequence of the AFP gene is represented in SEQ ID NO: 1 in a sequence list, nucleotide sequence of the GM-CSF gene is represented in SEQ ID NO: 2 in the sequence list, and nucleotide sequence of the IRES sequence is represented in SEQ ID NO: 3 in the sequence list. The dual-gene co-expression recombinant vector, which links the AFP gene and the GM-CSF gene through the IRES sequence, can simultaneously express alpha fetal protein and a granulocyte-macrophage colony stimulating factor in a same vector; and the recombinant vector, in immunogene therapy of liver cancer, not only can develop an immune regulating function of a cell factor but also can generate a specific anti-tumor effect targeted for the liver cancer.

The invention discloses a high-sensitivity antenatal screening kit of Down's Syndrome in pregnant women at second trimester as well as preparation and detection methods of the high-sensitivity antenatal screening kit. The kit comprises a kit body, AFP (Alpha Fetal Protein) and beta-HCG (Human Chorionic Gonadotropin) coated plates, AFP and beta-HCG standard products, AFP and beta-HCG quality control products, AFP and beta-HCG enzyme conjugates, sample diluent, light-emitting substrate liquids and concentrated scrubbing liquid. The preparation method comprises the following steps of: preparing monoclonal antibody coated plates of AFP and beta-HCG; preparing the standard products and the quality control products of AFP and beta-HCG; and preparing the enzyme conjugates of AFP and beta-HCG; and preparing the sample diluent, a light-emitting substrate liquid A, a light-emitting substrate liquid B, the concentrated scrubbing solution and the like. The high-sensitivity antenatal screening kit of the Down's Syndrome in the pregnant women at the second trimester has the advantages that the purpose of the antenatal screening of the Down's Syndrome in the pregnant women at the second trimester is realized, and the accordance rate with the clinic diagnosis reaches more than 99.7%; and the kit is high in sensitivity, strong in the specificity and accurate and reliable in result.

The invention discloses a colorimetric immunoassay method convenient for detecting a tumor marker. The colorimetric immunoassay method comprises preparation of a functional magnetic bead, preparation of a urease functional gold-nano probe and detection of contents of CEA in a standard solution and a sample. The method can be also used for detecting alpha fetal proteins (AFP), pancreatic oncofetal antigens (POA), isoenzyme (NSE), hormone (HCG) and the like. The method has the characteristics of simple operation, low cost and quick reaction; quantitative detection of an object to be detected can be carried out with only eyes or a simple ultraviolet-visible spectrophotometer, so that the colorimetric immunoassay method has an important significance for popularization of actual application of colorimetric immunoassay to aspects such as clinical diagnosis, medicine analysis and food safety.

The invention relates to an alpha fetal protein (AFP) kit based on an aptamer fluorescent probe, further relates to a method for measuring AFP concentration and reagent composition and components, and belongs to the technical field of medical detection and measurement. The kit mainly comprises the components of red blood cell lysis buffer, a phosphate buffer solution (PBS), an AFP standard and the AFP aptamer fluorescent probe. The AFP concentration is measured through blood sample splitting and mixed egg cultivation in conjunction with a fluorescent spectrophotometer. The alpha fetal protein kit has the advantages of simple sample treatment, easiness and convenience in operation, short detection time, high detection specificity, high flexibility, high detection result repeatability and the like.