The invention relates to preparation of cyclodextrin bonded stationary phase, in particular to a method for preparing cyclodextrin bonded stationary phase by taking click chemistry as bonding reaction process. The preparation method comprises two steps: firstly introducing triazo- or alkynyl onto the surface of silica gel by silane coupling agent to prepare triazo-silica gel derivative or alkynyl silica gel derivative, then carrying out 1,3-cycloaddition reaction on triazo- and alkynyl, namely, click chemistry, in aqueous solution at 20-80 DEG C for 24-72 hours, and binding cyclodextrin decorated with alkynyl or triazo- onto the surface of silica gel. The bonding reaction provided in the invention has high selectivity, can maintain cyclodextrin structure well, so that the preparation method is simple and broad in application range, the surface binding amount of the prepared cyclodextrin bonded stationary phase is high, is suitable to be as high performance liquid chromatography separation material, and especially suitable for separating strongly polar compound in hydrophilic liquid chromatography mode.