131 results about "Extracellular polysaccharide" patented technology

The invention discloses an oral care composition and a preparation method thereof, the oral care composition comprises a friction agent, a periodontal repair agent, a thickening agent, a humectant and a foaming agent, the friction agent comprises a dental plaque release agent, and the dental plaque release agent is configured to destroy a bacterial extracellular polysaccharide matrix in dental plaque; the periodontal repair agent comprises at least one of the following components: an inflammation inhibitor, a tissue repair promoter, a mucous membrane stimulation alleviator and a bacteriostatic agent. The oral care composition contains the dental plaque release agent and the periodontal repair agent, and can synergistically improve periodontal health.

The invention relates to the technical field of microorganisms, in particular to bifidobacterium bifidum BGLP1 capable of regulating sugar digestion and producing GLP-1 at high yield and application of the bifidobacterium bifidum BGLP1. The preservation number of the bifidobacterium bifidum BGLP1 is CGMCC (China General Microbiological Culture Collection Center) No.35718, and the bifidobacterium bifidum BGLP1 can be used for preparing the feed additive. The strain has the capability of inhibiting alpha-glucosidase and alpha-amylase; sTC-1 cells are stimulated to secrete GLP-1, and the blood glucose reducing pathway capacity of the intestinal tract is activated; the activity of pancreatic lipase is obviously inhibited, and the dietary fat absorption capability can be effectively reduced; redundant cane sugar is converted into exopolysaccharide, so that the probiotic function capability is improved; the capability of converting white fat into brown fat is promoted; macrophages are activated, and the body immune function is improved. In addition, the bifidobacterium bifidum BGLP1 also has relatively strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body.

The invention relates to the technical field of mineral processing, and particularly provides a combined reagent system for flotation and magnesium reduction of talc-containing copper-molybdenum ore and application of the combined reagent system. The combined reagent system comprises a flotation collecting agent (faint yellow to orange transparent liquid, 40%-50% of active ingredients and less than or equal to 3% of free alkali) with active ingredients in the formula 1 and a flotation depressing agent (light yellow powder, extracellular polysaccharide extract of xanthomonas, the weight-average molecular weight of 1000-1500000 Daltons and rich in hydroxyl, carboxyl and pyruvic acid groups) with a structural formula in the formula 2, and the flotation depressing agent comprises extracellular polysaccharide extract of xanthomonas, the weight-average molecular weight of xanthomonas and the weight-average molecular weight of xanthomonas and is rich in hydroxyl, carboxyl and pyruvic acid groups. Through the synergistic adsorption effect of the combined reagent, the flotation grade and the recovery rate of the copper sulfide molybdenum ore are remarkably improved under the condition that the pH is 8-11. The combined reagent system has the advantages of being high in flotation efficiency, high in selectivity, low in reagent cost, environmentally friendly, high in industrial applicability and the like.

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The invention discloses double-milk-source fermented milk of double-peak camel milk and brown milk and a preparation method of the double-milk-source fermented milk, and the preparation method comprises the following steps: mixing raw materials: mixing the double-peak camel milk and the brown milk according to a mass ratio to obtain double-milk-source mixed milk; pretreatment: sequentially carrying out homogenization treatment and heat treatment on the double-milk-source mixed milk; strain inoculation: inoculating the pretreated double-milk-source mixed milk with a fermentation strain, wherein the fermentation strain comprises a core strain and an auxiliary strain; the core strains are lactobacillus and streptococcus thermophilus which have tolerance to antibacterial components of the camel milk, and the core strains contain strains capable of producing exopolysaccharides. Through scientific matching of lactobacillus, streptococcus thermophilus and specific auxiliary strains which have tolerance to antibacterial components of the camel milk, the inhibition effect of natural antibacterial components such as lysozyme and lactoferrin in the camel milk on conventional strains is successfully overcome.

The invention discloses a thallus-exopolysaccharide co-embedded envelope state microcapsule and a preparation method thereof, and belongs to the technical field of microcapsule preparation. The preparation method comprises the following steps: (1) dissolving sodium alginate, pectin and whey protein isolate, stirring for 3-4 hours, and sterilizing to obtain a composite wall material solution; and (2) inoculating the activated bacterial liquid into a composite wall material solution, then adding the enveloping matrix exopolysaccharide, uniformly stirring and mixing, then adding a calcium chloride solution to form gel, then removing redundant gel, and cleaning to prepare the thallus-exopolysaccharide co-embedded enveloping state microcapsule. The co-embedded microcapsule prepared by the invention not only has good storage stability, high temperature resistance, osmotic pressure stress resistance and the like, but also can effectively promote the proliferation of thalli.

The invention discloses antibacterial mouthwash as well as a preparation method and application thereof, and belongs to the technical field of daily chemical products. According to the invention, egg white lysozyme, ferulic acid and grape seed procyanidine are taken as core components, and the mouthwash with an efficient bacteriostatic effect is developed. And the antibacterial rates of streptococcus mutans and porphyromonas gingivalis are 96.88% and 96.30% respectively. Researches show that bacterial biofilms can be destroyed by using the mouthwash, and the biofilm quantity is reduced by 53.7% after the bacterial biofilms are treated for 24 hours; extracellular polysaccharide is inhibited, the content of water-insoluble glucan is reduced by 62.3%, and synthesis of a key cariogenic matrix is hindered; the acid production capacity is regulated and controlled, the pH change of 24-hour culture is only 1.50, and xylitol competitively inhibits glycolysis and cooperates with ferulic acid to block bacterial metabolism. The mouthwash prepared by the invention avoids the problem of enzyme activity inhibition of the ionic surfactant of the traditional mouthwash, takes effect more quickly, and provides a basis for developing natural, safe and efficient functional mouthwash.

The invention relates to the technical field of microbial fermentation and food processing, and particularly discloses Weissella fusiformis JMF-006 as well as a mutagenesis method and application thereof. The invention provides a Weissella fusiformis JMF-006 with the exopolysaccharide yield of 20-25g / L. The Weissella fusiformis JMF-006 is obtained by breeding through a mutation breeding method of a high-yield EPS strain, and the mutation breeding method can also be used for culturing other high-yield EPS strains. The invention provides a strain composition, the strain composition comprises Weissella fusiformis JMF-006 or a high-yield EPS strain, other lactic acid bacteria strains (such as lactobacillus plantarum JMF-001 and lactobacillus casei JMF-002) can be further compounded, and the obtained strain composition has more excellent fermentation performance. When the Weissella fusiformis JMF-006, the high-yield EPS strain and the strain composition provided by the invention are applied to the fermentation process of food-derived drinks, a fermented product with stable properties and better flavor can be obtained, and the technical problems that the yield of extracellular polymeric substances is low, the fermentation performance is poor and the application range is narrow in an existing lactic acid bacteria starter are solved.

The invention relates to a method for purifying mine gushing water. The method comprises the following steps: mixing filamentous microalgae and to-be-treated mine gushing water for purification treatment. According to the method, filamentous microalgae are adopted to purify the to-be-treated mine gushing water, extracellular polysaccharide secreted by the filamentous microalgae growing in the mine gushing water can adsorb suspended solids in the mine gushing water, and meanwhile, the filamentous microalgae grow in the mine gushing water to be bent and wound algae filaments can also remove the suspended solids in the mine gushing water; therefore, the purification effect of the mine gushing water is effectively improved. Moreover, compared with traditional inorganic flocculants and organic polymer flocculants, the filamentous microalgae adopted in the invention have no chemical residues, and have less pollution to the environment and less toxicity to the ecological environment.

The invention discloses a freeze-dried tablet for improving the immunity of pets and a preparation process of the freeze-dried tablet, and belongs to the technical field of pet food manufacturing. The preparation process comprises the following steps: inoculating lactobacillus plantarum on the surface of sporoderm-broken ganoderma lucidum spore powder, producing stress conditions by intermittently spraying a nutrient solution, inducing thalli to proliferate and secrete exopolysaccharide (EPS) to form an active biological membrane, and obtaining a biological membrane-base material composite functional body; and mixing with the basic meat material, and freeze-drying to obtain the finished product. The probiotics are actively induced to form an endogenous biological membrane protection layer, so that the stress resistance of the probiotics is fundamentally improved. The prepared freeze-dried tablet is high in viable count (greater than or equal to 101CFU / g) and strong in gastric acid tolerance (survival rate gt, 80%), can be stored at normal temperature for a long time (survival rate gt, 79% after 12 months), can efficiently deliver viable bacteria to intestinal tracts of pets and exert an immunological enhancement function, and solves the technical problems that existing probiotic products are low in survival rate and limited in efficacy exertion.

The present application relates to the technical field of microorganisms, and particularly relates to a bifidobacterium BGLP1 capable of regulating sugar digestion and high GLP-1 production and application thereof. Bifidobacterium bifidum The bifidobacterium BGLP1 (CGMCC No. 35718) has the abilities of inhibiting alpha-glucosidase and alpha-amylase, stimulating STC-1 cells to secrete GLP-1 and activating the intestinal glucose-lowering pathway, significantly inhibiting pancreatic lipase activity and effectively reducing dietary fat absorption, converting excess sucrose into extracellular polysaccharides to increase the probiotic function, promoting the conversion of white fat into brown fat, and activating macrophages and improving the immune function of the body. In addition, the bifidobacterium BGLP1 has strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can successfully reach the human intestinal tract.

The invention belongs to the field of microorganisms, and particularly discloses lactobacillus bulgaricus for producing extracellular polysaccharide (EPS) and application of the lactobacillus bulgaricus. According to the invention, lactobacillus bulgaricus with high yield of exopolysaccharides is screened out from dairy fan fermentation acid water of traditional dairy products in Yunnan China, and the preservation number of the lactobacillus bulgaricus is CGMCC (China General Microbiological Culture Collection Center) NO: 29906. The content of crude extracellular polysaccharide extracted by the strain can reach 400mg / L, and the strain has great application value.

The present invention discloses C 14 The invention discloses a method for regulating acidophilic bacteria leaching, which belongs to the field of biological leaching technology. The invention finds that different types of AHL signal molecules can have different effects on the growth and leaching of acidophilic bacteria at different concentrations and different addition times. The signal molecule control experiment confirms that C 14 At certain concentrations, the HSL signal molecule can promote and enhance the production of extracellular polysaccharides and fulvic acid by the strain, as well as its ability to adhere to minerals in the later stages of leaching, thereby increasing the strain's growth rate and mineral leaching efficiency. By regulating the concentration of the signal molecule, the rate of acidophilic leaching and its growth can be adjusted. This method is simple to operate, has low production costs, and is environmentally friendly. This method solves the problem of difficult control during mixed acidophilic leaching, promotes the industrial development of biometallurgical technology, and improves leaching production efficiency.

This invention discloses a *Lactobacillus plantarum* YBE2 and its applications. The *Lactobacillus plantarum* provided by this invention, which produces a high amount of extracellular polysaccharides primarily composed of rhamnose, can simultaneously degrade different types of bile salts, including specifically hydrolyzing glycocholic acid. It also exhibits cholesterol-lowering and acute inflammation-relieving effects. Furthermore, *Lactobacillus plantarum* YBE2 can reduce fat deposition in the abdomen of caged broilers, increase intramuscular fat, promote fatty acid deposition in muscles, and reduce plasma total bile acids, triglycerides, and low-density lipoprotein cholesterol. This significantly improves lipid metabolism and muscle quality in poultry, and can be widely applied in caged broiler farming to promote healthy poultry farming and improve farming efficiency.

The present application discloses a kind of hibiscus compound extract, its preparation method and application.The composite extract includes hibiscus exosome, hibiscus traditional active ingredient, snowbank flower extract and microalgae extracellular polysaccharide, wherein the hibiscus exosome and hibiscus traditional active ingredient are simultaneously extracted from hibiscus raw materials by integrated extraction process.The preparation method includes raw material pretreatment, integrated extraction, separation and purification, synergistic compounding and post-processing, and two types of active substances are efficiently and synchronously obtained by optimizing solvent, temperature, time and purification parameters.The composite extract exhibits excellent anti-aging, anti-inflammatory and moisturizing effects in cosmetics, and has excellent effects of regulating intestinal flora and enhancing immunity in functional foods.The process produces significant synergistic effect by combining specific synergistic components, and the effect is much better than single component or simple mixture, realizing the maximization of plant resources and the synergistic promotion of efficacy.

This invention discloses a composite microbial agent for improving saline-alkali soil, its preparation method, and its application. The composite microbial agent is composed of *Frederick's chamaejasminoides* and *Zoococcus halophilus* with a viable count ratio of (0.2–2):1. Research shows that, under the same total viable count conditions, the composite microbial agent exhibits significantly higher salt tolerance, IAA production, siderophore production capacity, ACC deaminase activity, phosphorus solubility, nitrogen fixation capacity, and extracellular polysaccharide production compared to single-strain agents, demonstrating a significant synergistic effect. The composite agent can also be combined with the salt-tolerant plant *Arundo donax* to construct a synergistic remediation system for saline-alkali land. This system significantly reduces the pH and sodium ion content of saline-alkali soil, improves soil nutrient availability, and significantly promotes *Arundo donax* growth and biomass accumulation, providing an innovative technical solution for rapid, efficient, and sustainable ecological restoration and agricultural utilization of saline-alkali land.

The present invention belongs to the field of biomedical materials technology, and specifically relates to a probiotic hydrogel and its preparation method and application. The probiotic hydrogel comprises a hydrogel substrate formed by oxidized methylmalonamide hyaluronic acid; and the hydrogel substrate is cross-linked with probiotics and extracellular polysaccharides secreted by the probiotics, wherein the probiotics are Lactobacillus acidophilus. L. acidipiscis 10851 , the extracellular polysaccharide is L. acidipiscis 10851 The probiotic hydrogel of the present invention is suitable for various traumas and surgical wounds, and can stop bleeding, reduce wound infection and promote wound healing.

This invention provides the application of *Ackermania guangxiensis* extracellular polysaccharide in the preparation of anti-aging products, belonging to the technical field of microbial polysaccharide pharmaceutical applications. This invention extracts extracellular polysaccharides from *Ackermania guangxiensis* using the TCA precipitation method. Animal experiments show that *Ackermania guangxiensis* extracellular polysaccharide can effectively reduce MDA content in aging model mice while increasing SOD activity; it improves the thinning of the dermal layer, reduction and loosening of collagen fibers, and other signs of aging in model mice, and increases the hydroxyproline content in the skin; furthermore, it can activate the expression of BDNF and TrKB genes in the mouse hippocampus and alleviate problems such as slow movement, cognitive impairment, and memory decline in model mice. Therefore, the *Ackermania guangxiensis* extracellular polysaccharide provided by this invention has good anti-aging and antioxidant activity and can be used to prepare various anti-aging products.

The invention provides a strain of lactobacillus plantarum ZENIYOUTH-01 and application thereof, and belongs to the technical field of probiotics and application thereof. The fermented whole grain prepared by adopting the lactobacillus plantarum disclosed by the invention effectively retains nutritional ingredients such as vitamin B, vitamin E, nicotinic acid, mineral substances and the like in the whole grain; abundant enzyme systems secreted by lactobacillus plantarum are utilized to degrade macromolecular proteins, dietary fibers and the like in the raw materials to generate bioactive peptides, short-chain fatty acids, exopolysaccharides and flavor compounds, release of functional components such as polyphenols, flavonoid compounds and the like is promoted, the contents of protein peptides, lactic acid, acetic acid, citric acid and beta-glucan are increased, and the nutritional value of the beverage is improved. The traditional Chinese medicine composition can effectively proliferate intestinal effective microbial communities and promote intestinal peristalsis, and has a remarkable effect in the aspect of improving constipation.

The application belongs to the technical field of microorganisms, and discloses a bacillus amyloliquefaciens D189 with high yield of extracellular polysaccharide. The bacillus amyloliquefaciens D189 is preserved in China Center for Type Culture Collection on April 29, 2021, and the preservation number is CCTCC NO: M2021484. The bacillus amyloliquefaciens D189 provided in the application has high yield of extracellular polysaccharide, the yield of the crude extracellular polysaccharide EPS-OD is 131.31+ / -2.13 g / L after optimization, the extracellular polysaccharide EPS is obtained by purifying the crude EPS-OD through a DEAE ion exchange column, and the composition of the crude EPS-OD and the EPS is determined. The extracellular polysaccharide of the bacillus amyloliquefaciens D189 has good alpha-glucosidase inhibitory capacity and pancreatic lipase inhibitory capacity, and good total bile acid and cholesterol micelle binding capacity in vitro, so that the extracellular polysaccharide and the lipid-lowering and glucose-lowering product have good application prospects.

The invention provides a biochar-based microbial composition and application thereof in soda saline-alkali soil, and belongs to the technical field of soil improvement. The invention provides a biochar-based microbial composition. Microorganisms for producing exopolysaccharides and the exopolysaccharides are fixed in a graded pore structure of biochar. According to the method, biochar with a hierarchical pore structure is taken as a carrier, microorganisms with exopolysaccharide (EPS) secretion capacity are colonized in the deep positions of mesopores and macropores in the biochar in a vacuum impregnation manner, and strains are subjected to in-situ synthesis in advance in the pores of the biochar and secrete a large amount of EPS. According to the biochar-based microbial composition, through dual protection of a biochar physical barrier and an EPS chemical barrier, the survival rate of strains is remarkably increased, and the soil improvement effect is achieved.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus safranin* and its application in the preparation of flocculants. The strain is *Bacillus safranin*. (Bacillu s safensis)BS‑RCEES‑ 2025‑001 The strain was deposited at the China General Microbiological Culture Collection Center on December 30, 2025, with accession number [accession number missing]. CGMCCNo.37242。 The present invention is based on Bacillus sarfus. (Bacillus safensis)BS‑RCEES‑2025‑001 The strain secretes extracellular polysaccharides that have a strong flocculation effect, and the flocculation rate of the wastewater treated with the flocculant prepared from it is 80% to 94%.

The application discloses a lactobacillus kefiri subsp. kefiri IMAU13289 and application, belongs to the technical field of microorganisms, provides a lactobacillus kefiri subsp. Lactobacillus Lactobacillus kefiranofaciens subsp. kefiranofaciens ) IMAU13289, which is preserved in the China General Microbiological Culture Collection Center on December 4, 2024, and the address is No. 1, Xibaixili, Chaoyang District, Beijing, and the preservation number is CGMCC No. 32897. The lactobacillus kefiri subsp. kefiri IMAU13289 has the ability of high yield of extracellular polysaccharide, a fast lactic acid production rate, and neomycin resistance, and can be used for preparing fermented milk with excellent coagulation characteristics and excellent storage characteristics.

This invention relates to an extracellular polysaccharide of *Lactobacillus delbrueckii* subsp. bulgaricus CB-01 with immunomodulatory effects and its applications. The extracellular polysaccharide is prepared by a method comprising the following steps: inoculating *Lactobacillus delbrueckii* subsp. bulgaricus CB-01 into sterilized sugar-containing milk for fermentation; centrifuging the fermentation broth; collecting the supernatant; precipitating with alcohol; collecting the crude polysaccharide precipitate; redissolving the precipitate in water; mixing it with trichloroacetic acid solution; centrifuging to collect the supernatant; precipitating again with alcohol; redissolving the precipitate; and dialyzing to obtain the extracellular polysaccharide. In this invention, by inoculating CB-01 into a specific sugar-containing milk for fermentation, it was unexpectedly discovered that CB-01 can efficiently secrete extracellular polysaccharides in the fermented milk matrix. The prepared *Lactobacillus delbrueckii* subsp. bulgaricus extracellular polysaccharide exhibits significant immunomodulatory capabilities, and its use in preparing immune-enhancing foods, health products, or medical nutritional products shows great promise.

The application provides a pseudomonas koreensis WF3 and a saline-alkali soil microbial fertilizer, a preparation method and application thereof, and belongs to the technical field of biological composting.The preparation method of the saline-alkali soil microbial fertilizer provided by the application comprises the following steps: (1) configuring a fertilizer carrier comprising matured organic fertilizer, humic acid, attapulgite powder and polyglutamic acid; (2) inoculating the pseudomonas koreensis WF3 into the fertilizer carrier, and carrying out sealed fermentation to obtain the saline-alkali soil microbial fertilizer.The pseudomonas koreensis WF3 has the abilities of producing extracellular polysaccharides and organic acids, and can survive in a strong saline-alkali environment; the fertilizer carrier is fermented by the pseudomonas koreensis WF3; the prepared microbial organic fertilizer can effectively improve the soil organic carbon, total nitrogen and water-stable large aggregate (>2.5mm) content, increase the corn yield, reduce the soil pH and total salt content, and has a remarkable improvement effect on the saline-alkali soil.

The application discloses a plant Lactiplantibacillus plantarum Yi-BC5 with a preservation number of GDMCC No: 67799, which shows strong inhibition on Pectobacterium carotovorum and Penicillium digitatum in vitro and in vivo, can significantly reduce the incidence and lesion diameter of pepper bacterial soft rot and citrus green mold; the Lactiplantibacillus plantarum Yi-BC5 can produce extracellular polysaccharide and other antibacterial metabolites, and the bacterial body and / or bacterial metabolite can be used for preparing products for inhibiting Pectobacterium carotovorum and / or Penicillium digitatum, and can be used for preparing products for preventing and treating bacterial diseases (including but not limited to pepper bacterial soft rot) and / or fungal diseases (including but not limited to citrus green mold) of fruits and vegetables. The Lactiplantibacillus plantarum Yi-BC5 can simultaneously control bacterial diseases and fungal diseases of fruits and vegetables after harvesting, has excellent disease control performance, provides an excellent antagonistic bacterial resource for postharvest preservation of fruits and vegetables, and lays a foundation for subsequent development of a new type of microbial bactericide.

A hydrogel composition comprises: a first gelling agent comprising alginate, carrageenan, and gellan gum; and a second gelling agent comprising succinoglycan.

This invention belongs to the field of microbial technology, specifically relating to a method for preparing and applying a fused Weissella extracellular polysaccharide. The extracellular polysaccharide of this invention is composed of glucose monosaccharide, and the glucose linkages include terminal glucose t-Glc(p), 1→4 linked glucose, 1→4,6 linked glucose, 1→3,6 linked glucose, and 1→2,6 linked glucose, with a relative molar ratio of (8~8.5):(1~1.2):(81~82):(7~7.5):(1~1.2):(0.5~1). It possesses excellent water-holding capacity, oil-holding capacity, emulsifying properties, and emulsion stability, and can be used as a highly efficient moisturizer, emulsifier, emulsion stabilizer, cosmetic additive, pharmaceutical additive, and food additive, showing broad application prospects in the food, cosmetic, and pharmaceutical fields.

The application belongs to the technical field of skin care product preparation, and discloses a natural polysaccharide composition, a preparation method and application thereof. The natural polysaccharide composition is composed of the following components in percentage by mass: Pseudoalteromonas ferment product extract 50-70%, locust bean gum 10-15%, cassia gum 5-10%, and psyllium seed gum 15-25%. The Pseudoalteromonas ferment product extract refers to extracellular polysaccharide produced by fermentation of Pseudoalteromonas mucosa DSM15203; the locust bean gum is obtained by extraction and mannanase enzymolysis of seeds of locust trees; the cassia gum is obtained by extraction and mannanase enzymolysis of seed endosperm of cassia plants; and the psyllium seed gum is obtained by extraction and xylanase enzymolysis of seed husks of psyllium.

The invention provides a method for improving a lactobacillus plantarum metabolite through illumination pretreatment, and belongs to the field of microbial fermentation. According to the method disclosed by the invention, a strain suspension is subjected to visible light irradiation with specific illumination and duration before lactobacillus plantarum is activated. Compared with a control group which is not subjected to light treatment, the lactobacillus plantarum treated by the method disclosed by the invention has the advantages that the intracellular key enzyme activity is enhanced, the thallus concentration is almost not influenced, the antioxidant activity is improved, and meanwhile, the yield of exopolysaccharides is also effectively improved.