95 results about "Extracellular polysaccharide" patented technology

The invention relates to the technical field of mineral processing, and particularly provides a combined reagent system for flotation and magnesium reduction of talc-containing copper-molybdenum ore and application of the combined reagent system. The combined reagent system comprises a flotation collecting agent (faint yellow to orange transparent liquid, 40%-50% of active ingredients and less than or equal to 3% of free alkali) with active ingredients in the formula 1 and a flotation depressing agent (light yellow powder, extracellular polysaccharide extract of xanthomonas, the weight-average molecular weight of 1000-1500000 Daltons and rich in hydroxyl, carboxyl and pyruvic acid groups) with a structural formula in the formula 2, and the flotation depressing agent comprises extracellular polysaccharide extract of xanthomonas, the weight-average molecular weight of xanthomonas and the weight-average molecular weight of xanthomonas and is rich in hydroxyl, carboxyl and pyruvic acid groups. Through the synergistic adsorption effect of the combined reagent, the flotation grade and the recovery rate of the copper sulfide molybdenum ore are remarkably improved under the condition that the pH is 8-11. The combined reagent system has the advantages of being high in flotation efficiency, high in selectivity, low in reagent cost, environmentally friendly, high in industrial applicability and the like.

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The invention discloses antibacterial mouthwash as well as a preparation method and application thereof, and belongs to the technical field of daily chemical products. According to the invention, egg white lysozyme, ferulic acid and grape seed procyanidine are taken as core components, and the mouthwash with an efficient bacteriostatic effect is developed. And the antibacterial rates of streptococcus mutans and porphyromonas gingivalis are 96.88% and 96.30% respectively. Researches show that bacterial biofilms can be destroyed by using the mouthwash, and the biofilm quantity is reduced by 53.7% after the bacterial biofilms are treated for 24 hours; extracellular polysaccharide is inhibited, the content of water-insoluble glucan is reduced by 62.3%, and synthesis of a key cariogenic matrix is hindered; the acid production capacity is regulated and controlled, the pH change of 24-hour culture is only 1.50, and xylitol competitively inhibits glycolysis and cooperates with ferulic acid to block bacterial metabolism. The mouthwash prepared by the invention avoids the problem of enzyme activity inhibition of the ionic surfactant of the traditional mouthwash, takes effect more quickly, and provides a basis for developing natural, safe and efficient functional mouthwash.

The invention relates to the technical field of microbial fermentation and food processing, and particularly discloses Weissella fusiformis JMF-006 as well as a mutagenesis method and application thereof. The invention provides a Weissella fusiformis JMF-006 with the exopolysaccharide yield of 20-25g / L. The Weissella fusiformis JMF-006 is obtained by breeding through a mutation breeding method of a high-yield EPS strain, and the mutation breeding method can also be used for culturing other high-yield EPS strains. The invention provides a strain composition, the strain composition comprises Weissella fusiformis JMF-006 or a high-yield EPS strain, other lactic acid bacteria strains (such as lactobacillus plantarum JMF-001 and lactobacillus casei JMF-002) can be further compounded, and the obtained strain composition has more excellent fermentation performance. When the Weissella fusiformis JMF-006, the high-yield EPS strain and the strain composition provided by the invention are applied to the fermentation process of food-derived drinks, a fermented product with stable properties and better flavor can be obtained, and the technical problems that the yield of extracellular polymeric substances is low, the fermentation performance is poor and the application range is narrow in an existing lactic acid bacteria starter are solved.

The present application relates to the technical field of microorganisms, and particularly relates to a bifidobacterium BGLP1 capable of regulating sugar digestion and high GLP-1 production and application thereof. Bifidobacterium bifidum The bifidobacterium BGLP1 (CGMCC No. 35718) has the abilities of inhibiting alpha-glucosidase and alpha-amylase, stimulating STC-1 cells to secrete GLP-1 and activating the intestinal glucose-lowering pathway, significantly inhibiting pancreatic lipase activity and effectively reducing dietary fat absorption, converting excess sucrose into extracellular polysaccharides to increase the probiotic function, promoting the conversion of white fat into brown fat, and activating macrophages and improving the immune function of the body. In addition, the bifidobacterium BGLP1 has strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can successfully reach the human intestinal tract.

This invention discloses a *Lactobacillus plantarum* YBE2 and its applications. The *Lactobacillus plantarum* provided by this invention, which produces a high amount of extracellular polysaccharides primarily composed of rhamnose, can simultaneously degrade different types of bile salts, including specifically hydrolyzing glycocholic acid. It also exhibits cholesterol-lowering and acute inflammation-relieving effects. Furthermore, *Lactobacillus plantarum* YBE2 can reduce fat deposition in the abdomen of caged broilers, increase intramuscular fat, promote fatty acid deposition in muscles, and reduce plasma total bile acids, triglycerides, and low-density lipoprotein cholesterol. This significantly improves lipid metabolism and muscle quality in poultry, and can be widely applied in caged broiler farming to promote healthy poultry farming and improve farming efficiency.

The present application discloses a kind of hibiscus compound extract, its preparation method and application.The composite extract includes hibiscus exosome, hibiscus traditional active ingredient, snowbank flower extract and microalgae extracellular polysaccharide, wherein the hibiscus exosome and hibiscus traditional active ingredient are simultaneously extracted from hibiscus raw materials by integrated extraction process.The preparation method includes raw material pretreatment, integrated extraction, separation and purification, synergistic compounding and post-processing, and two types of active substances are efficiently and synchronously obtained by optimizing solvent, temperature, time and purification parameters.The composite extract exhibits excellent anti-aging, anti-inflammatory and moisturizing effects in cosmetics, and has excellent effects of regulating intestinal flora and enhancing immunity in functional foods.The process produces significant synergistic effect by combining specific synergistic components, and the effect is much better than single component or simple mixture, realizing the maximization of plant resources and the synergistic promotion of efficacy.

This invention discloses a composite microbial agent for improving saline-alkali soil, its preparation method, and its application. The composite microbial agent is composed of *Frederick's chamaejasminoides* and *Zoococcus halophilus* with a viable count ratio of (0.2–2):1. Research shows that, under the same total viable count conditions, the composite microbial agent exhibits significantly higher salt tolerance, IAA production, siderophore production capacity, ACC deaminase activity, phosphorus solubility, nitrogen fixation capacity, and extracellular polysaccharide production compared to single-strain agents, demonstrating a significant synergistic effect. The composite agent can also be combined with the salt-tolerant plant *Arundo donax* to construct a synergistic remediation system for saline-alkali land. This system significantly reduces the pH and sodium ion content of saline-alkali soil, improves soil nutrient availability, and significantly promotes *Arundo donax* growth and biomass accumulation, providing an innovative technical solution for rapid, efficient, and sustainable ecological restoration and agricultural utilization of saline-alkali land.

This invention provides the application of *Ackermania guangxiensis* extracellular polysaccharide in the preparation of anti-aging products, belonging to the technical field of microbial polysaccharide pharmaceutical applications. This invention extracts extracellular polysaccharides from *Ackermania guangxiensis* using the TCA precipitation method. Animal experiments show that *Ackermania guangxiensis* extracellular polysaccharide can effectively reduce MDA content in aging model mice while increasing SOD activity; it improves the thinning of the dermal layer, reduction and loosening of collagen fibers, and other signs of aging in model mice, and increases the hydroxyproline content in the skin; furthermore, it can activate the expression of BDNF and TrKB genes in the mouse hippocampus and alleviate problems such as slow movement, cognitive impairment, and memory decline in model mice. Therefore, the *Ackermania guangxiensis* extracellular polysaccharide provided by this invention has good anti-aging and antioxidant activity and can be used to prepare various anti-aging products.

The invention provides a strain of lactobacillus plantarum ZENIYOUTH-01 and application thereof, and belongs to the technical field of probiotics and application thereof. The fermented whole grain prepared by adopting the lactobacillus plantarum disclosed by the invention effectively retains nutritional ingredients such as vitamin B, vitamin E, nicotinic acid, mineral substances and the like in the whole grain; abundant enzyme systems secreted by lactobacillus plantarum are utilized to degrade macromolecular proteins, dietary fibers and the like in the raw materials to generate bioactive peptides, short-chain fatty acids, exopolysaccharides and flavor compounds, release of functional components such as polyphenols, flavonoid compounds and the like is promoted, the contents of protein peptides, lactic acid, acetic acid, citric acid and beta-glucan are increased, and the nutritional value of the beverage is improved. The traditional Chinese medicine composition can effectively proliferate intestinal effective microbial communities and promote intestinal peristalsis, and has a remarkable effect in the aspect of improving constipation.

The application belongs to the technical field of microorganisms, and discloses a bacillus amyloliquefaciens D189 with high yield of extracellular polysaccharide. The bacillus amyloliquefaciens D189 is preserved in China Center for Type Culture Collection on April 29, 2021, and the preservation number is CCTCC NO: M2021484. The bacillus amyloliquefaciens D189 provided in the application has high yield of extracellular polysaccharide, the yield of the crude extracellular polysaccharide EPS-OD is 131.31+ / -2.13 g / L after optimization, the extracellular polysaccharide EPS is obtained by purifying the crude EPS-OD through a DEAE ion exchange column, and the composition of the crude EPS-OD and the EPS is determined. The extracellular polysaccharide of the bacillus amyloliquefaciens D189 has good alpha-glucosidase inhibitory capacity and pancreatic lipase inhibitory capacity, and good total bile acid and cholesterol micelle binding capacity in vitro, so that the extracellular polysaccharide and the lipid-lowering and glucose-lowering product have good application prospects.

The invention provides a biochar-based microbial composition and application thereof in soda saline-alkali soil, and belongs to the technical field of soil improvement. The invention provides a biochar-based microbial composition. Microorganisms for producing exopolysaccharides and the exopolysaccharides are fixed in a graded pore structure of biochar. According to the method, biochar with a hierarchical pore structure is taken as a carrier, microorganisms with exopolysaccharide (EPS) secretion capacity are colonized in the deep positions of mesopores and macropores in the biochar in a vacuum impregnation manner, and strains are subjected to in-situ synthesis in advance in the pores of the biochar and secrete a large amount of EPS. According to the biochar-based microbial composition, through dual protection of a biochar physical barrier and an EPS chemical barrier, the survival rate of strains is remarkably increased, and the soil improvement effect is achieved.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus safranin* and its application in the preparation of flocculants. The strain is *Bacillus safranin*. (Bacillu s safensis)BS‑RCEES‑ 2025‑001 The strain was deposited at the China General Microbiological Culture Collection Center on December 30, 2025, with accession number [accession number missing]. CGMCCNo.37242。 The present invention is based on Bacillus sarfus. (Bacillus safensis)BS‑RCEES‑2025‑001 The strain secretes extracellular polysaccharides that have a strong flocculation effect, and the flocculation rate of the wastewater treated with the flocculant prepared from it is 80% to 94%.

The application discloses a lactobacillus kefiri subsp. kefiri IMAU13289 and application, belongs to the technical field of microorganisms, provides a lactobacillus kefiri subsp. Lactobacillus Lactobacillus kefiranofaciens subsp. kefiranofaciens ) IMAU13289, which is preserved in the China General Microbiological Culture Collection Center on December 4, 2024, and the address is No. 1, Xibaixili, Chaoyang District, Beijing, and the preservation number is CGMCC No. 32897. The lactobacillus kefiri subsp. kefiri IMAU13289 has the ability of high yield of extracellular polysaccharide, a fast lactic acid production rate, and neomycin resistance, and can be used for preparing fermented milk with excellent coagulation characteristics and excellent storage characteristics.

This invention relates to an extracellular polysaccharide of *Lactobacillus delbrueckii* subsp. bulgaricus CB-01 with immunomodulatory effects and its applications. The extracellular polysaccharide is prepared by a method comprising the following steps: inoculating *Lactobacillus delbrueckii* subsp. bulgaricus CB-01 into sterilized sugar-containing milk for fermentation; centrifuging the fermentation broth; collecting the supernatant; precipitating with alcohol; collecting the crude polysaccharide precipitate; redissolving the precipitate in water; mixing it with trichloroacetic acid solution; centrifuging to collect the supernatant; precipitating again with alcohol; redissolving the precipitate; and dialyzing to obtain the extracellular polysaccharide. In this invention, by inoculating CB-01 into a specific sugar-containing milk for fermentation, it was unexpectedly discovered that CB-01 can efficiently secrete extracellular polysaccharides in the fermented milk matrix. The prepared *Lactobacillus delbrueckii* subsp. bulgaricus extracellular polysaccharide exhibits significant immunomodulatory capabilities, and its use in preparing immune-enhancing foods, health products, or medical nutritional products shows great promise.

The application provides a pseudomonas koreensis WF3 and a saline-alkali soil microbial fertilizer, a preparation method and application thereof, and belongs to the technical field of biological composting.The preparation method of the saline-alkali soil microbial fertilizer provided by the application comprises the following steps: (1) configuring a fertilizer carrier comprising matured organic fertilizer, humic acid, attapulgite powder and polyglutamic acid; (2) inoculating the pseudomonas koreensis WF3 into the fertilizer carrier, and carrying out sealed fermentation to obtain the saline-alkali soil microbial fertilizer.The pseudomonas koreensis WF3 has the abilities of producing extracellular polysaccharides and organic acids, and can survive in a strong saline-alkali environment; the fertilizer carrier is fermented by the pseudomonas koreensis WF3; the prepared microbial organic fertilizer can effectively improve the soil organic carbon, total nitrogen and water-stable large aggregate (>2.5mm) content, increase the corn yield, reduce the soil pH and total salt content, and has a remarkable improvement effect on the saline-alkali soil.

The application discloses a plant Lactiplantibacillus plantarum Yi-BC5 with a preservation number of GDMCC No: 67799, which shows strong inhibition on Pectobacterium carotovorum and Penicillium digitatum in vitro and in vivo, can significantly reduce the incidence and lesion diameter of pepper bacterial soft rot and citrus green mold; the Lactiplantibacillus plantarum Yi-BC5 can produce extracellular polysaccharide and other antibacterial metabolites, and the bacterial body and / or bacterial metabolite can be used for preparing products for inhibiting Pectobacterium carotovorum and / or Penicillium digitatum, and can be used for preparing products for preventing and treating bacterial diseases (including but not limited to pepper bacterial soft rot) and / or fungal diseases (including but not limited to citrus green mold) of fruits and vegetables. The Lactiplantibacillus plantarum Yi-BC5 can simultaneously control bacterial diseases and fungal diseases of fruits and vegetables after harvesting, has excellent disease control performance, provides an excellent antagonistic bacterial resource for postharvest preservation of fruits and vegetables, and lays a foundation for subsequent development of a new type of microbial bactericide.

A hydrogel composition comprises: a first gelling agent comprising alginate, carrageenan, and gellan gum; and a second gelling agent comprising succinoglycan.

This invention belongs to the field of microbial technology, specifically relating to a method for preparing and applying a fused Weissella extracellular polysaccharide. The extracellular polysaccharide of this invention is composed of glucose monosaccharide, and the glucose linkages include terminal glucose t-Glc(p), 1→4 linked glucose, 1→4,6 linked glucose, 1→3,6 linked glucose, and 1→2,6 linked glucose, with a relative molar ratio of (8~8.5):(1~1.2):(81~82):(7~7.5):(1~1.2):(0.5~1). It possesses excellent water-holding capacity, oil-holding capacity, emulsifying properties, and emulsion stability, and can be used as a highly efficient moisturizer, emulsifier, emulsion stabilizer, cosmetic additive, pharmaceutical additive, and food additive, showing broad application prospects in the food, cosmetic, and pharmaceutical fields.

The invention provides a method for improving a lactobacillus plantarum metabolite through illumination pretreatment, and belongs to the field of microbial fermentation. According to the method disclosed by the invention, a strain suspension is subjected to visible light irradiation with specific illumination and duration before lactobacillus plantarum is activated. Compared with a control group which is not subjected to light treatment, the lactobacillus plantarum treated by the method disclosed by the invention has the advantages that the intracellular key enzyme activity is enhanced, the thallus concentration is almost not influenced, the antioxidant activity is improved, and meanwhile, the yield of exopolysaccharides is also effectively improved.

The invention relates to the technical field of microorganisms, and particularly discloses a bifidobacterium breve BB18 and an application of an exopolysaccharide BB18-EPS (extracellular polysaccharide) produced by the bifidobacterium breve BB18. The strain is preserved in the China General Microbiological Culture Collection Center on June 9, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34621. The exopolysaccharide BB18-EPS secreted by the strain can be used as a food additive for gel modification of shrimp myofibrillar protein (MP), and the product performance is remarkably improved. Experiments prove that BB18-EPS drives ordered crosslinking of myosin through a hydrophobic effect, promotes transformation of a secondary structure of MP to alpha-helix, and optimizes the recombination efficiency of a molecular chain; meanwhile, through interaction of hydroxyl, sugar residues and water molecules, the density of a gel network is enhanced, and the thermal stability and the deformation resistance are improved. The composite gel system can be applied to the fields of minced penaeus vannamei products and the like, and the product texture and the processing yield are effectively improved.

The application provides a multifunctional microalgae-based hydrogel wound dressing and a preparation method and application thereof, and belongs to the field of hydrogel dressings. Microalgae are embedded in a hydrogel network formed by modified gelatin and cross-linked sodium alginate, and a multifunctional alginate hydrogel is constructed for chronic wound repair of diabetes. The selected microalgae can realize in-situ synthesis and uniform distribution of small-sized gold nanoparticles on the surface thereof during co-culture with high-concentration gold ions, stimulate high-efficiency secretion of extracellular polysaccharides by the microalgae, and maintain high cell activity. The prepared dressing is helpful for relieving a local hypoxic microenvironment of a wound and promoting vascular regeneration, can effectively remove excess active oxygen at a wound site, and can induce macrophages to polarize to M2 type; in addition, the gold nanoparticles in the hydrogel network have good antibacterial performance.

This invention relates to a Bacillus thuringiensis strain for controlling fall armyworm. The strain is IPPO68, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37126. The extracellular polysaccharides produced by this strain exhibit insecticidal activity against fall armyworm.

This invention discloses a strain of Codonopsis pilosula endophytic fungus that produces extracellular polysaccharides and its applications. This Codonopsis pilosula endophytic fungus is a short-spore bacterium, named... Brevibacillus schisleri CpRG-1. The Codonopsis pilosula endophytic bacteria of the present invention exhibit excellent ability to produce extracellular polysaccharides, producing high concentrations of extracellular polysaccharides, which can greatly reduce the cost of extracting polysaccharides from the medicinal herb Codonopsis pilosula; at the same time, the extracellular polysaccharides produced by the Codonopsis pilosula endophytic bacteria not only have antibacterial and hypoglycemic activities, but also can promote the healing of diabetic foot ulcers and improve the intestinal microbiota, providing new ideas for the development of antibacterial drugs, diabetes prevention foods and drugs, and diabetic foot ulcer treatment foods and drugs.

The invention discloses a drought-resistant salt-tolerant growth-promoting strain and application thereof. The bacterial strain is bacillus parlicheniformis XT33, and the preservation number of the bacterial strain is CCTCC (China Center For Type Culture Collection) NO: M 20252789. The strain has remarkable drought resistance, salt resistance and multiple growth promoting functions, and can still grow well under the stress of 20% PEG and 5% NaCl; the strain can secrete heteroauxin, produce exopolysaccharide and form a biological membrane, and has the capabilities of dissolving phosphorus, producing siderophores and the like. The invention also provides a microbial agent containing the strain, a preparation method of the microbial agent, and a method for promoting seed germination and seedling growth of plants under drought and / or salt stress by soaking seeds with the microbial agent. The strain has composite functions and strong stress resistance, and provides an excellent strain resource for developing a green microbial inoculant for agricultural production in arid and saline-alkali areas.

The application discloses a kind of porous high-dispersion Ag / Cu / Cu2O composite material to enhance the antibacterial characteristics of microbial extracellular polysaccharide (EPS) and be applied to food packaging, belongs to the field of antibacterial material.The application aims to solve the technical problem that the application of lactic acid bacterial extracellular polysaccharide in food preservation, anti-infection medical dressing and other fields is limited.The application uses PVP as carbon source and template agent, uses Cu (NO3) 2 as Cu source, and uses a small amount of AgNO3 as Ag source to synthesize porous high-dispersion Ag / Cu / Cu2O nanocomposite, which is simple in method and cheap in raw materials.After connecting EPS with Ag / Cu / Cu2O nanocomposite, the bactericidal characteristics of EPS-Ag / Cu / Cu2O composite are greatly improved compared with EPS-Ag and EPS-Cu / Cu2O.The Ag / Cu / Cu2O nanocomposite of the application can greatly reduce the use amount of noble metal Ag and exhibit very excellent antibacterial characteristics, so it has more extensive practical application value.

The invention relates to the technical field of microbial fermentation. The invention provides application of clinacanthus nutans leaf powder in preparation of a fermentation culture medium for improving the yield and antioxidant activity of ganoderma lucidum exopolysaccharides and a method for improving the yield and antioxidant activity of ganoderma lucidum exopolysaccharides by using the fermentation culture medium. According to the invention, clinacanthus nutans leaf powder with a specific concentration is added into a ganoderma lucidum liquid fermentation culture medium, so that the yield of the exopolysaccharide is synergistically improved, the chemical structure of the exopolysaccharide is changed, and the antioxidant activity of the product is also remarkably enhanced due to the change of the structure of the exopolysaccharide. The invention provides an innovative method for efficiently producing high-activity fungal polysaccharide, and the method also has a wide application prospect in the fields of functional foods and medicines.

The present application provides a grifola frondosa liquid culture medium and its culture method and application, relates to the fermentation technical field.The present application provides a grifola frondosa liquid culture medium, including glucose, proteose peptone, KH2PO4, MgSO4, vitamin B1, gibberellin and tyrosol, the culture medium can significantly improve grifola frondosa growth and the secretion of extracellular polysaccharide, and can be used for the preparation of food, medicine and health care product.

This invention relates to a Bacillus subtilis ( Bacillus subtle Y32, with accession number CGMCC No. 34819, is a strain that produces heat-resistant and highly stable xylanase, as well as various hydrolases such as β-mannanase and protease. It also produces extracellular polysaccharides, inhibits Botrytis cinerea, and is acid-resistant, resistant to porcine bile salts, and resistant to 10% NaCl. The optimal temperature for the xylanase produced by this strain is 55℃, and the optimal pH is 6.0. Under these conditions, the half-life is 12.5 h, and the relative enzyme activity remains above 80% after 3 h of incubation, demonstrating good thermal stability. This strain can also increase the yield of cucumbers and Pinellia ternata, and increase the egg production rate of 70-week-old Hy-Line Brown hens from 70.4% to 90.1%. This strain has promising applications in agriculture, food processing, and cosmetics production.

Provided is a method for infecting a bacterium, in which a host bacterium is exposed to a phage depolymerase and a bacteriophage. The depolymerase catalyzes degradation of at least one cell-surface or extracellular polysaccharide of the host bacterium, thereby increasing the susceptibility of the host bacterium to infection by the bacteriophage. The method may be used to increase production of the bacteriophage and / or to increase the host range of the bacteriophage. Further provided is use of a phage depolymerase selected from B5, As11, TaPaz, APK2, APK37, and IME200, or a composition comprising the same, for increasing the susceptibility of an A. baumannii to infection by a bacteriophage. The A. baumannii may be an AB030, MRSN 843, MRSN 1183, MRSN 7446, MRSN 15088, MRSN 24008, MRSN 31159, MRSN 31468, or MRSN 32866 strain.