Method for highly effective production and separating purification of Chinese caterpillar fungus extracellular polysaccharide

A high-efficiency technology for exopolysaccharides, applied in the field of rapid separation and purification of exopolysaccharides, can solve the problems of destroying polysaccharide structures, increasing industrial production costs, and destroying biological activities, achieving high purity and good uniformity

Inactive Publication Date: 2008-06-18
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Application Information

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Problems solved by technology

It not only destroys the structure of polysaccharides and greatly destroys their biological ac

Method used

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  • Method for highly effective production and separating purification of Chinese caterpillar fungus extracellular polysaccharide
  • Method for highly effective production and separating purification of Chinese caterpillar fungus extracellular polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In a 5-liter culture bottle, inoculate Cordyceps militaris, the inoculation amount is 10%, the amount of the culture medium is: 3 liters, according to the culture medium, yeast extract powder 1.5%, brown sugar 2.5%, male silkworm moth oil 5%, KNO 3 0.95%, MgSO 4 0.2%, KH 2 PO 4 0.2% formula, static culture for 8 days. The obtained culture solution is filtered with ordinary filter paper, the filtrate is centrifuged at 12000rmp / min, and the filtrate is filtered with a 0.22 μm filter membrane, and the filtrate is continuously filtered with an ultrafiltration membrane with a molecular weight of 0.5 million Daltons, and the molecular weight cut-off value is 1, 5. 1. Different ultrafiltration membranes of 100,000 Daltons are used to concentrate the obtained filtrate by graded high-pressure ultrafiltration, and vacuum freeze-dry the obtained intercepted concentrated solution to obtain a single component with uniform molecular weight distribution and purity ≥ 95%.

Embodiment 2

[0023] In a 50 liter fermenter, inoculate Cordyceps militaris, the inoculum amount is 6%, the amount of the fermenter medium is: 30 liters, according to the medium yeast extract powder 0.8%, brown sugar 1.5%, male silkworm moth oil 4%, KNO 3 0.85%, MgSO 4 0.1%, KH 2 PO 4 0.1% formula, static culture for 6 days. The obtained culture solution is filtered with ordinary filter paper, the filtrate is centrifuged at 12000rmp / min, and the filtrate is filtered with a 0.22 μm filter membrane, and the filtrate is continuously filtered with an ultrafiltration membrane with a molecular weight of 0.5 million Daltons, and the molecular weight cut-off value is 1, 5. 1. Different ultrafiltration membranes of 100,000 Daltons are used to concentrate the obtained filtrate by graded high-pressure ultrafiltration, and vacuum freeze-dry the obtained intercepted concentrated solution to obtain a single component with uniform molecular weight distribution and purity ≥ 95%.

Embodiment 3

[0025] In a 100-liter fermenter, inoculate Cordyceps militaris, the inoculum amount is 8%, the amount of the fermenter medium is: 60 liters, according to the medium yeast extract powder 1%, brown sugar 2.0%, male silkworm moth oil 3%, KNO 3 0.88%, MgSO 4 0.15%, KH 2 PO 4 0.15% formula, static culture for 7 days. The obtained culture solution is filtered with ordinary filter paper, the filtrate is centrifuged at 12000rmp / min, and the filtrate is filtered with a 0.22 μm filter membrane, and the filtrate is continuously filtered with an ultrafiltration membrane with a molecular weight of 0.5 million Daltons, and the molecular weight cut-off value is 1, 5. 1. Different ultrafiltration membranes of 100,000 Daltons are used to concentrate the obtained filtrate by graded high-pressure ultrafiltration, and vacuum freeze-dry the obtained intercepted concentrated solution to obtain a single component with uniform molecular weight distribution and purity ≥ 95%.

[0026] Beneficial ...

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Abstract

The main aim of the invention is to provide a cultivation method of producing aweto extracellular polysaccharide in a highly efficient industrialized way and use the membrane dialysis technology to rapidly purify extracellular polysaccharide single component with biological activity. And the purity is more than or equal to 95 percent. The purify technology does not use any organic solvent and chemical reagent to ensure that the chemical structure of the aweto extracellular polysaccharide is not destroyed, thereby maintaining the biological activity.

Description

technical field [0001] The invention belongs to the field of biological fermentation and separation and purification, and in particular relates to a fermentation culture method for efficiently producing extracellular polysaccharides by submerged fermentation of Cordyceps mycelium and a method for rapid separation and purification of extracellular polysaccharides. Background technique [0002] Polysaccharides are important components in animal cell membranes, plant and microbial cell walls. The research on polysaccharides was first carried out in the 1940s, but it was in the 1960s that people paid great attention to polysaccharides as broad-spectrum immune enhancers. After more than 40 years of continuous development, people have a new understanding of polysaccharides, an important life substance, making this subject one of the most active research fields in life sciences. [0003] At present, researches on polysaccharides from Cordyceps sinensis mainly focus on fruiting bod...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12N1/14C08B37/00
Inventor 计东风范雷法钟石李有贵
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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