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Ensiferadhaerens and application thereof

The technology of a sticky arrow fungus and an application method is applied in the application field of improving the germination rate of soybean, and can solve the problems of plant inhibition, low land yield, insufficient agricultural comprehensive production capacity and the like

Inactive Publication Date: 2013-01-02
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When the salt concentration reaches a certain value, the normal life activities of plant cells will be destroyed, and the growth of plants will be inhibited. Therefore, soil salinization will cause low land output rate and serious shortage of comprehensive agricultural production capacity, which restricts the development of agricultural production.
There is no research report on E. adhaerens as a growth-promoting bacterium in plant rhizosphere

Method used

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  • Ensiferadhaerens and application thereof
  • Ensiferadhaerens and application thereof
  • Ensiferadhaerens and application thereof

Examples

Experimental program
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Effect test

example 1

[0015] Example 1: Isolation, screening, identification and biological characteristics of nitrogen-fixing bacterial strains in plant rhizosphere soil

[0016] 1. Strain Isolation

[0017] The soil was collected from Nanjing City, Jiangsu Province. Take 2g of soil and add it to 18mL sterile water containing 10 glass beads, shake for 5min, and after standing for 10min, take 10ml of the suspension and add it to 250ml of mineral salt medium containing 1% glucose. The composition of the mineral salt medium is: 1.36 g / L KH 2 PO 4 , 2.13g / L Na 2 HPO 4 , 0.5 g / L MgSO 4 ·7H 2 O and 10mL / L metal ionic liquid, pH 7.5. The metal ionic liquid composition is: 0.40 g / L CaCl 2 2H 2 O, 0.30 g / L H 3 BO 3 , 0.04 g / L CuSO 4 ·5H 2 O, 0.10 g / L KI, 0.20 g / L FeSO 4 ·7H 2 O, 0.40 g / L MnSO 4 ·7H 2 O, 0.20 g / L NaMoO 4 2H 2 O and 10.0 mL / L concentrated hydrochloric acid. The samples were incubated at 30°C for 1 month in a shaker with a rotation speed of 220rpm. Take 10ml of the sus...

example 2

[0025] Example two: E. adhaerens PGPR Activity Determination of CGMCC 6315 Strain

[0026] Refer to the method reported by Bric et al. in 1991 to determine the content of indole acetic acid IAA; refer to the method reported by Alexander and Zuberer in 1991 to measure the secretion of siderophore siderophore on the chrome azure solid plate; refer to the method reported by Reeves et al. in 1983 to determine salicylic acid The content of SA and 2,3-dihydroxybenzoic acid DHBA; the determination of exopolysaccharide EPS content refers to the method reported by Mody et al. in 1989; the release of phosphorus, hydrogen cyanide and ammonia is detected according to the method reported by Schippers et al. in 1990 . The measurement results show that E. adhaerens CGMCC 6315 strain produced 8.2 μg / mL indole acetic acid, 5.7 mg / mL exopolysaccharide after 24 hours of culture, 6.9 μg / mL salicylic acid and 0.48 μg / mL 2,3-dihydroxybenzoic acid after 48 hours of culture; E. adhaerens C...

example 3

[0027] Example three : Inoculation on soybean surface under salt stress conditions E. adhaerens CGMCC 6315 can promote its germination

[0028] Pick several soybeans of the same size and soak them in tap water for 6 h. Sterilize with 70% ethanol for 30S and rinse with sterile water to remove ethanol. E. adhaerens CGMCC 6315 bacteria were cultured in LB liquid medium for 16 hours, centrifuged, washed with sterile water, suspended with different concentrations of sodium chloride solution, and the concentration of bacteria was adjusted to 10 8 Colony forming units (CFU). Soak the above-mentioned treated soybeans in NaCl solutions of different concentrations containing the strains for 30 min.

[0029] Put a layer of sterilized filter paper at the bottom of a 9 cm sterile petri dish, and add 4 mL of NaCl solutions of different concentrations mentioned above. Put 20 above-mentioned treated soybeans into each petri dish, put the petri dish into an incubator, and cul...

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Abstract

The invention discloses Ensiferadhaerens CGMCC 6315 which is a bacterium having a nitrogen-fixing capability. The strain is also a plant growth promoting rhizobacterium (PGPR), and can produce indoleacetic acid (IAA), extracellular polysaccharide (EPS), siderophore, salicylic acid (SA), 2,3-dihydroxybenzoic acid (DHBA), hydrogen cyanide (HCN) and ammonia. Under the stress condition of a salt containing sodium chloride, soybeans inoculated with the E.adhaerens CGMCC 6315 can have a higher germination rate in comparison with a control group which is not inoculated with the strain.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to nitrogen-fixing bacteria and plant rhizosphere growth-promoting bacterium Sagitta agglutinans Ensifer adhaerens Application of CGMCC 6315 to increase soybean germination rate under salt stress. Background technique [0002] Plant rhizosphere growth-promoting bacteria PGPR is a kind of rhizosphere microorganisms that can promote crop vigor and increase yield, and inhibit plant pathogenic bacteria and harmful microorganisms in the rhizosphere. PGPR is an important resource bank of biofertilizers and biopesticides, and its screening, development and application have received more and more attention. The mechanism of PGPR promoting plant vigor can be divided into two ways: direct and indirect. The direct promotion effect includes the production of plant growth hormone, nitrogen fixation, and enhancement of the absorption and utilization of nutrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/202C12R1/01A23L11/20
Inventor 戴亦军王颖翟闪周广灿葛峰袁生
Owner NANJING NORMAL UNIVERSITY
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