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Variovorax and application thereof

A technology of greedy bacteria and plant growth-promoting bacteria, applied in the field of greedy bacteria and its application, can solve the problems of saline-alkali hazards, low crop yield, low soil nutrients, etc., to promote absorption, increase soil mineral nitrogen content, The effect of increasing nitrogen use efficiency

Active Publication Date: 2013-09-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Huang-Huai-Hai Plain with the largest distribution area of ​​fluvo-aquic soil suffers from frequent droughts and floods, and there are still saline-alkali hazards. In addition, the soil nutrients are low or lacking.

Method used

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  • Variovorax and application thereof
  • Variovorax and application thereof
  • Variovorax and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] First prepare the following three media.

[0033] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-7.2, sterilized at 121°C, 20min.

[0034] LB liquid medium: without agar, other conditions are the same as above.

[0035] Ashby nitrogen-free medium: mannitol 10g, KH 2 PO 4 0.29, MgSO 4 ·7H 2 O0.29, NaCI0.29, CaSO 4 ·2H 2 O0.29, CaCO 3 5g, pH7.2, 121°C sterilization, 20min.

[0036] Inorganic phosphate bacteria culture medium (PKO medium): Tricalcium phosphate 5g, glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.3g, magnesium sulfate heptahydrate 0.3g, potassium chloride 0.3g, manganese sulfate 0.03g, heptahydrate Ferrous sulfate 0.03g, pH7.0 agar 20g, distilled water 1000ml, pH7.0~7.2. Sterilize at 121℃, 20min.

[0037] Pick the roots of the fluvial soil taken from Banqiao, Nanjing, weigh 10g and place it in a 250ml Erlenmeyer flask containing 100ml of sterilized water, in a shaker, 30℃, 150r·min -1 Shake for 20 minutes ...

Embodiment 2

[0050] Aerobic test

[0051] Pour the sterilized LB medium into 3 sterilized test tubes, about 2 / 3 of the place, on the aseptic operation table, use the inoculation needle to pick up the inclined surface cultured JX14, and puncture and inoculate the above medium (It must be punctured to the bottom of the tube). Incubate at 30°C and observe the results in 3 to 7 days. Those growing on the surface of the agar column are aerobic bacteria, such as those growing along the puncture line are anaerobes or facultative anaerobes. The test results show that JX14 (CGMCC No.5623) colonies grow along the surface of the agar column, and the aseptic colonies grow within the puncture line, which is strictly aerobic.

[0052] Determination of catalase

[0053] Put 1 drop of 3% H on a clean glass slide 2 O 2 , Take 1 ring of JX14 (CGMCC No.5623) LB slant culture 18~24h, in H 2 O 2 Medium smear, if there are bubbles, it is positive, otherwise it is negative. The test results show that JX14 (CGMCC No...

Embodiment 3

[0079] JX14 (CGMCC No.5623) has an obvious growth-promoting effect on peanuts, which will be explained by pot experiment below.

[0080] Collect fresh fluvo-aquic soil in a soil layer of 0-20cm under natural conditions, pass a 5mm sieve, 200g soil per pot, plant peanuts, adjust the water content to 60% of the maximum water holding capacity in the field, sample after 30 days, use a root scanner (LA1600+ Scanner, Canada) After scanning the root image, use the root analysis software (Winrhizo2003b, Canada) to analyze the relevant root index, and use the flow analyzer to determine the soil mineral nitrogen content. The molybdenum blue colorimetric method was used to determine the soil available phosphorus content.

[0081] Peanut seeds: Surface peanut seeds are disinfected with 20% hydrogen peroxide for 20 minutes, rinsed with sterile water several times, germination is accelerated for 2 days, and seeds with consistent germination are selected for use.

[0082] Inoculation treatment: In...

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Abstract

The invention discloses variovorax and application of the variovorax and belongs to the field of agriculture microbe. Plant growth-promoting rhizobacteria JX14 is conserved in the common microbe center of China microbial culture collection management committee on Dec 20, 2011; the class name is variovorax (variovorax sp.), and the conservation number is CGMCC No.5623. The strain can fix nitrogen and grow by utilizing insoluble phosphate as a phosphorus source, so the utilization rate of the fertilizer is improved, and root development of plants and absorption of fertilizer are facilitated. The variovorax has a good growth-promoting effect on peanut; nitrogen fixation facilitates growth development of the peanut, and the utilization rate of the peanut on phosphatic fertilizer is higher due to the phosphate-solubilizing ability.

Description

Technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a greedy phage and its application. Background technique [0002] Fluvo-aquic soil is the soil formed by river sediments affected by groundwater movement and farming activities. It is named after the night tide phenomenon. In China, it is mostly distributed in the alluvial plains of the middle and lower reaches of the Yellow River and the plains of Jiangsu and Anhui to the south, and river, lake plains and deltas in the middle and lower reaches of the Yangtze River. The fluvo-aquic soil distribution area is flat, with deep soil layers, abundant water and heat resources, and wide variety of planting. It is the main dryland soil in my country and is rich in grain and cotton. However, the Huanghuaihai Plain, which has the largest distribution area of ​​fluvo-aquic soil, has frequent droughts and floods, and there are still salt-alkali hazards. In addition, soil nutrients are low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C05F11/08A01G1/00A01G7/00C12R1/01
Inventor 李辉信姜瑛徐文思张振李方卉胡锋焦加国徐莉刘满强陈小云
Owner NANJING AGRICULTURAL UNIVERSITY
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