204 results about "Metal ion sequestering" patented technology

The invention discloses a soluble alkaline salt scale inhibitor and a preparation method thereof, and belongs to the technical field of oilfield chemistry. The soluble alkaline salt scale inhibitor is prepared from amphiphilic modified graphene oxide, a surfactant, inorganic salt and deionized water, the amphiphilic modified graphene oxide contains a large amount of carboxyl groups, sulfonic acid groups, hydroxyl groups and the like, and has good metal ion chelating property; meanwhile, through the hydrophobic association effect and hydrogen-bond interaction between modified graphene grafted long carbon chains, the bonding probability between sodium ions of the soluble saline-alkali salt and bicarbonate radicals, carbonate radicals and chloride ions is further weakened, and through the synergistic effect of the amphiphilic modified graphene oxide, the surfactant and the inorganic salt, the salt scale inhibition effect is further improved; the method is especially suitable for soluble alkali salt water quality conditions of high temperature, high alkali and high salinity, can solve the problems of oilfield water injection, fracturing flowback strata and wellbore salt deposition, and has a good popularization prospect.

The invention discloses a nano-antibacterial agent for treating biofilm infection through Prussian blue and a preparation method and application thereof.According to the nano-antibacterial agent, hollow Prussian blue (HPB) serves as a carrier to load a substance with anti-inflammatory and anti-oxidation effects, a metal polyphenol network structure is formed through chelating of tannic acid (TA) modified by hyaluronic acid (HA) and high-valence metal ions, and the nano-antibacterial agent is used for treating biofilm infection through Prussian blue. And the MPNs are packaged to obtain the product. All the components of the nano antibacterial agent have a synergistic effect, MPNs formed by chelating TA modified by hyaluronic acid and high-valence metal ions in the antibacterial agent have good photo-thermal performance, and light energy can be converted into heat energy for photo-thermal therapy. According to the invention, the nanoparticles for photo-thermal antisepsis and anti-inflammation are constructed, so that comprehensive treatment of anti-biofilm and anti-inflammatory effect is realized, and healing of infected parts is promoted. And the antibacterial agent has good biocompatibility and high biological safety, and is very suitable for replacing antibiotics to treat biological membrane infection.

The invention provides silk fibroin hydrogel containing divalent bioactive metal ions and a preparation method of the silk fibroin hydrogel, and belongs to the technical field of soft and hard tissue repair and medical dressings. The invention provides a silk fibroin hydrogel preparation method, which comprises: S1, dissolving degummed silk by using a dissolving agent to prepare a silk fibroin stock solution; s2, dialyzing the silk fibroin stock solution in water dissolved with divalent metal salt to obtain a silk fibroin composite solution; s3, carrying out heat preservation treatment on the silk fibroin composite solution, then applying shearing force, and incubating to obtain the silk fibroin hydrogel containing the metal ions. The preparation method of the silk fibroin hydrogel is simple, the pH value does not need to be adjusted in the preparation process, a gelatinizing agent does not need to be added, two cross-linking mechanisms of beta-folding cross-linking of a silk fibroin hydrophobic chain segment are promoted under the action of shearing force by chelating and cross-linking a silk fibroin hydrophilic chain segment through divalent metal ions, so that a silk fibroin aqueous solution is rapidly gelatinized, and the silk fibroin hydrogel is prepared. And meanwhile, one or more divalent metal ions can be compounded according to expected purposes, and good operability, biological safety and multifunctionality are achieved.

A photocurable composition includes quantum dots, quantum dot precursor materials, a chelating agent, one or more monomers, and a photoinitiator. The quantum dots are selected to emit radiation in a first wavelength band in the visible light range in response to absorption of radiation in a second wavelength band in the UV or visible light range. The second wavelength band is different than the first wavelength band. The quantum dot precursor materials include metal atoms or metal ions corresponding to metal components present in the quantum dots. The chelating agent is configured to chelate the quantum dot precursor materials. The photoinitiator initiates polymerization of the one or more monomers in response to absorption of radiation in the second wavelength band.

The invention relates to the technical field of cleaning agents, and discloses a biodegradable environment-friendly cleaning agent, which comprises: a composite surfactant system comprising at least one nonionic surfactant, an ampholytic surfactant and a chelating functional surfactant; the plant extract oil system comprises limonene and a eucalyptus oil mixture; the functional particle system comprises nanoscale enzyme microcapsules and slow-release type oxidized particles; the natural polysaccharide stable network system comprises at least two polysaccharide thickeners and a bio-based cross-linking agent, and the assistant system comprises a metal ion chelating agent, a buffer acid system and water. By introducing sodium polyaspartate as a green chelating surfactant, efficient chelating of calcium and magnesium ions under a hard water condition is realized, the cleaning power is remarkably improved, water stain residues are reduced, and the cleaning performance with wider adaptability is obtained.

The invention relates to the technical field of OLED (Organic Light Emitting Diode) manufacturing process etching processes, in particular to a silver etching solution for an OLED manufacturing process as well as a preparation method and application of the silver etching solution. The silver etching liquid comprises nitric acid, phosphoric acid, a buffer agent, a metal ion chelating agent and an ionic liquid inhibitor, the ionic liquid inhibitor comprises 1-butyl-3-methylimidazole phosphate, the metal ion chelating agent is selected from at least one of pyromellitic acid, diethylenetriamine pentaacetic acid and ethylenediamine tetramethylene phosphoric acid, and the buffer agent is selected from at least one of sodium chloride, sodium chloride and sodium chloride. The buffering agent comprises acetic acid, and all the components are prepared according to a specific mass ratio. The etching solution can balance the etching rate of silver and indium tin oxide, avoid silver residues and black spot defects, prolong the service life of the solution, reduce the use of a volatile buffer agent, adapt to the OLED fine manufacturing process, improve the product yield and reduce the production cost, and is suitable for etching of a metal substrate in the OLED manufacturing process.

The invention relates to the technical field of anaerobic adhesives, and particularly discloses a structural anaerobic adhesive for bonding high-temperature-resistant magnetic steel and a preparation method thereof.The anaerobic adhesive is prepared from, by mass, 60%-80% of urethane acrylate, 10%-20% of methacrylate, 0.01%-1% of polymerization inhibitor, 0.1%-5% of metal ion chelating agent, 0.1%-5% of accelerant and 0.1%-5% of auxiliary accelerant. 1-15% of a bismaleimide heat-resistant auxiliary agent, 1-10% of a core-shell structure impact modifier, and 1-5% of an initiator. By adding the heat-resistant additive and the impact modifier, the temperature resistance and toughness of the anaerobic adhesive are improved, and the anaerobic adhesive is especially suitable for magnetic steel bonding in a high-temperature environment.

The present invention relates to a bimetallic layered hydroxide / phytic acid synergistic flame-retardant nylon 6 composite material and its preparation method and application, belonging to the technical field of flame retardant material synthesis. The composite material is prepared from nylon 6, bimetallic layered hydroxide and phytic acid. Wherein, the phosphate functional group in the phytic acid can chelate with the metal ion in the bimetallic layered hydroxide, thereby forming a novel flame retardant. When combustion occurs, the layered structure and thermal decomposition products such as metal oxides, metal phosphates, and carbon-containing aromatic network structure substances in the flame retardant help to suppress the release of smoke and toxic gases, and the release of metal ions and phosphorus-containing free radicals helps to suppress the spread of fire. Compared with pure nylon 6, the peak heat release rate and total heat release rate of the composite material are greatly reduced, the limiting oxygen index is greatly increased, and the vertical combustion test reaches V-0 level. At the same time, the raw material sources in the preparation method of the composite material are abundant, the operation is simple, and it is suitable for scaled-up production.

The invention provides a stable acetylcysteine injection and a preparation method thereof. The acetylcysteine injection provided by the invention only contains a small amount of metal ion chelating agent, and can still improve the content of related substances in the preparation and obviously reduce the content of hydrogen sulfide in the preparation by using a novel package and performing terminal high-temperature sterilization under the condition of a pre-cleaning process. More importantly, in the long-term storage process of the acetylcysteine injection provided by the invention, the content of hydrogen sulfide presents a trend of continuously reducing.

This application relates to a deoxyribonucleic acid and pseudovirus preservation solution, its preparation method and application. The deoxyribonucleic acid and pseudovirus preservation solution includes: a buffer solution with a final concentration of 5 mM to 50 mM, a surfactant with a volume percentage of 0.05% to 1.0%, trehalose with a final concentration of 50 mM to 500 mM, a metal ion chelator with a final concentration of 0.5 mM to 5 mM, betaine with a final concentration of 100 mM to 1000 mM, and mannitol with a mass percentage of 1% to 10%. Each specific component in the above-mentioned deoxyribonucleic acid and pseudovirus preservation solution cooperates with each other at a specific concentration, which has the effect of stabilizing the state and structure of nucleic acids. It can achieve the preservation of nucleic acids and pseudoviruses without ultra-low temperature, and can stably preserve nucleic acids and pseudoviruses for up to 12 months at 30°C without affecting subsequent detection tests.

The technology of the present disclosure generally relates to the field of power storage devices, and more specifically to a solid electrolyte, an electrode, a solid-state battery, and a method for producing the same. An aspect of the present disclosure relates to a process for producing a solid electrolyte, comprising the steps of: - contacting, in the presence of a catalyst: - a polymer precursor compound comprising polyethylene glycol (PEG) and / or polytetrahydrofuran (PTHF), wherein the polymer precursor compound comprises on average per polymer precursor molecule, -at least 2.0 alkoxysilyl functional groups, with a functionality of at least 2.0, preferably at least 2.5, preferably at least 3.0; and / or -at least 1.0 alkoxysilyl functional groups, with a functionality of at least 3.0; - optionally, an organo-tri-alkoxy silane, an organo-tetra-alkoxy silane, and / or a mixture thereof comprising a metal ion (M+) chelating moiety being a cyanurate, a crown ether, an aza-crown ether, a thia-crown ether, a cryptand, a carbamate, a cyano group, a thiocyanate group, or a metallacrown,, - a metal (M+) salt, preferably does the metal salt comprises Li+, Na+, Mg+, Ca+, Al+, and / or a combination thereof, preferably lithium (Li+); - a solvent, preferably an organic solvent; and, - optionally, an ionically conductive compound; thereby obtaining a solid electrolyte; - optionally, drying the obtained solid electrolyte.

The invention discloses soluble powder for preventing and treating chicken necrotic enteritis, and belongs to the technical field of veterinary medicine preparations. The soluble powder preparation comprises the following components: 30-80% of penicillin V potassium, 1-20% of a beta-lactam inhibitor, 0.5-6% of a pH regulator, 0-10% of an anti-caking agent, 0.02-0.5% of a metal ion chelating agent and the balance of a filler, totaling 100%. The soluble powder is used for drinking water of poultry, and the content of penicillin V potassium in an aqueous solution of the soluble powder is still kept at 99% or above after 12 hours and is still kept at 95% or above after 24 hours. The water solution of the soluble powder does not block a water drinking line in clinical use, so that the clinical use effect and convenience are greatly improved.

The invention discloses a process for preparing a multifunctional liquid fertilizer from tylosin waste liquid, and belongs to the technical field of fertilizer preparation. The method comprises the following steps: pretreating the tylosin waste liquid to obtain pretreated waste liquid; adjusting the pH value of the pretreated waste liquid to obtain the pH-adjusted waste liquid; sterilizing and filtering the waste liquid subjected to pH regulation to obtain pure treatment liquid; the pure treatment liquid is subjected to nutrient substance blending, and the multifunctional liquid fertilizer prepared from the tylosin waste liquid is obtained. According to the method, a tylosin degradation catalyst is synthesized in the waste liquid in the pretreatment and pH adjustment processes, tylosin in the waste liquid is degraded in the sterilization process, and soil pollution caused by residual tylosin in the waste liquid is prevented while the steps are shortened; in the blending of the nutrient substances, the acidic substances are added to chelate metal ions, so that the release of the nutrient substances in the fertilizer is delayed, the resource utilization rate is increased, and the fertilizer is worthy of wide-range popularization.

The invention relates to a stable melogabalin besylate solid preparation containing an antioxidant, and belongs to the technical field of pharmaceutical preparations. The invention specifically provides a melogabalin besylate solid preparation, the solid preparation comprises melogabalin besylate and an antioxidant, the antioxidant is an amino acid antioxidant, and the solid preparation also comprises a metal ion chelating agent. The chemical stability of the pharmaceutical preparation provided by the invention is far better than that of a commercially available preparation under a high-temperature condition, the preparation process of the tablet is powder direct compression, the process is simple, the preparation process is stable, and the obtained pharmaceutical preparation is relatively low in related substance level and stable during storage.

The invention relates to the technical field of medical dressings, and discloses a compound hydrogel dressing based on recombinant mussel mucin and a preparation method of the compound hydrogel dressing. The compound hydrogel based on the recombinant mussel mucin is prepared from the following raw material components in parts by weight: 0.01 to 0.3 part of the recombinant mussel mucin, 0.1 to 2.0 parts of a thickening agent, 10 to 21 parts of a polysaccharide composition, 1 to 8 parts of a humectant, 0.01 to 0.5 part of a pH (Potential of Hydrogen) regulator, 0.05 to 0.2 part of a preservative, 0.05 to 0.2 part of a metal ion chelating agent and 68 to 88 parts of purified water, the polysaccharide composition is prepared from glucan, chitosan and hyaluronic acid. The polysaccharide is compounded into the hydrogel of the recombinant mussel mucoprotein to play a role synergistically, and the polysaccharide and the recombinant mussel mucoprotein are closely combined and cross-linked, so that the adhesion of the compound hydrogel dressing is effectively improved, wound healing is promoted, the anti-inflammatory effect is improved, and meanwhile, a good protective barrier environment is formed for wound repair and healing.

The invention provides an efficient and environment-friendly printing and dyeing sewage decolorizing agent which is mainly prepared from the following raw materials in parts by mass: 10-17 parts of nanoscale silicon dioxide, 5-12 parts of concentrated sulfuric acid, 8-13 parts of a polyacrylamide emulsion, 5-17 parts of lignosulfonate, 3-10 parts of a metal ion chelating agent and 10-25 parts of deionized water. The decolorizing agent provided by the invention has good adsorption performance and chemical stability, and is low in cost and high in decolorizing efficiency on printing and dyeing sewage.

The present invention relates to a preparation method of a barium strontium sulfate descaling agent, comprising the following steps: adding a prepared additive solution to a prepared chelating agent alkaline solution, fully mixing and dissolving the solution, and adjusting the pH to alkaline, thereby obtaining the barium strontium sulfate descaling agent. The present invention has the following beneficial effects: the preparation method of the present invention can significantly improve the descaling effect of the descaling agent on barium strontium sulfate scale, increase the contact area with metal ions, thereby improving the overall descaling effect, effectively destroying the formation of barium strontium sulfate scale, and the descaling agent still maintains a stable descaling effect under high temperature conditions, and has broad industrial application prospects. The chelating agent used not only has good metal ion chelating effect, can effectively complex barium, strontium ions, and destroy the formation of barium sulfate and strontium sulfate crystals, but also has the characteristics of being stable under high temperature and high pH value, being suitable for complex industrial conditions, and performing well in neutral and alkaline water, and is also more environmentally friendly.

The invention relates to the technical field of daily chemicals, and particularly discloses a dishwashing liquid for a low-temperature dishwasher and a preparation method of the dishwashing liquid. The dishwashing liquid for the low-temperature dishwashing machine comprises a colored stain removal synergistic composition, in the dishwashing liquid for the low-temperature dishwashing machine, the mass percentage of the colored stain removal synergistic composition is 30-40%; the colored stain removal synergistic composition is prepared by mixing sodium glucoheptonate and trisodium methylglycine diacetate according to a mass ratio of (1-3): 1. The synergistic composition for removing the colored stains has excellent low-temperature stability and high-efficiency metal ion chelation capacity, the dishwashing liquid containing the above content can efficiently remove the colored stains such as tea stains, coffee stains and fruit stains in a low-temperature environment, the decontamination effect of the dishwashing liquid in the low-temperature environment is improved, and the dishwashing liquid has the advantages of being environmentally friendly and free of toxic and side effects. And the defect that a liquid detergent cannot clean the stains is overcome.

The invention provides a vegetable protein composition and a production method thereof, and belongs to the technical field of protein. Comprising the following steps: S1, removing bean substance resistance factors; s2, carrying out mixed fermentation on the microalgae, bean substances and protein mulberry, and separating to obtain protein peptide; s3, chelating metal ions of the protein peptide; s4, condensing the protein peptide chelated with the metal ions; s5, preparation of carboxylated pullulan polysaccharide; s6, preparation of hydrophobic modified carboxylated pullulan polysaccharide; and S7, preparing the plant protein composition. The prepared plant protein composition has good physiological activity, through the embedding technology, the storage stability, heat resistance, acid resistance and moisture resistance of protein peptide are improved, growth and development of livestock are promoted, the meat quality is improved, and the plant protein composition has wide application prospects.

The invention provides a composition of 14 amino acids and glucose stored in a double-chamber bag and a preparation method of the composition. The composition of 14 amino acids and glucose stored in the double-chamber bag does not contain sulfite antioxidants and metal ion chelating agents. The preparation method comprises the following steps: pretreating production equipment by adopting a pretreatment solution containing inorganic acid, organic acid and ammonium salt, and then preparing liquid medicine, filtering, making bags, filling, sealing outer bags, sterilizing and the like. Double-chamber bag packaging is adopted, so that the Maillard reaction of amino acid and glucose in the production and storage process can be avoided, meanwhile, the aluminum migration risk in the packaging material is reduced, and the clinical medication safety and effectiveness of the product are improved. During clinical use, liquid medicine in the two cavities is mixed through extrusion, the bioavailability of all the components can be synergistically improved, meanwhile, osmotic pressure is reduced, and clinical infusion compliance is improved.

The invention relates to a cerebroprotein peptide and casein hydrolysate composition and application thereof in improving sleep and memory, and belongs to the technical field of biological protein peptides. According to the preparation method disclosed by the invention, Maillard modification of the casein enzymolysis primary product is realized by screening the enzymolysis protease of the bovine milk casein and screening the carbonyl compound which is subjected to Maillard reaction with the casein enzymolysis primary product, so that the reducing power, the metal ion chelating capacity and the antioxidant capacity of the casein hydrolysate are effectively improved; furthermore, free radicals in the body are removed, oxidative stress reaction is relieved, and a good foundation is laid for improving sleep quality. The brain protein peptide and the casein hydrolysate are compounded, so that the sleep latency can be effectively shortened, the sleep rate can be increased, the sleep can be effectively improved, the content of dopamine and noradrenaline in hypothalamus can be reduced, the content of 5-hydroxytryptamine and the content of aminobutanoin can be increased, and the brain protein peptide composition has good effects of relieving and treating sleep disorders and improving memory.

The invention relates to perillaseed oil nanoemulsion eye drops as well as a preparation method and application thereof, and belongs to the technical field of medicines. The perillaseed oil nanoemulsion eye drops comprise the following raw material components in parts by mass: 1-20 parts of perillaseed oil, 0.1-10 parts of an emulsifier, 0-10 parts of a co-emulsifier, 0.05-3 parts of a metal ion chelating agent and 1-5 parts of an osmotic pressure regulator. By adding the metal ion chelating agent and controlling the preheating temperature, the problem that perillaseed oil is easy to oxidize is solved. According to the perilla oil nanoemulsion eye drops, the perilla oil serves as an effective component to exert the drug effect and also serves as an oil phase, meanwhile, the particle size of the nanoemulsion is accurately controlled by adjusting the variety of the co-emulsifier, and the safety and stability of the perilla oil nanoemulsion eye drops are further improved. The perillaseed oil eye drops provided by the invention can effectively relieve the symptoms of xerophthalmia and asthenopia, and a new medication choice is provided for treating xerophthalmia and myopia.

This invention discloses a method, system, equipment, and medium for activating and promoting the release of humic acid from silkworm excrement. The method includes: mechanochemical synergistic crystal-breaking pretreatment, i.e., dry ball milling to destroy the physical encapsulation structure and induce a local alkaline microenvironment under chemical solvent-free conditions; stepwise enzymatic hydrolysis bridging activation, utilizing the acid buffering properties of silkworm excrement, sequentially adding uricase, cellulase / hemicellulase, and laccase or manganese peroxidase to form a natural pH fluctuation curve; directed microbial induction and release promotion, inoculating with Bacillus amyloliquefaciens and Bacillus lentigines for liquid fermentation to achieve metal ion chelation and surface charge regulation; and finally, low-temperature drying and molding to obtain the product. This invention also discloses the system, computer equipment, and storage medium for implementing this method. This invention does not require the addition of external acids, alkalis, or chemical chelating agents, shortens the total cycle to 2-4 days, and the obtained product has a high water-soluble humic acid content, which enhances the resistance to refixation after application to the soil.

Provided is a sheet material configured so that a metal-organic framework is not likely to become separated. This sheet material comprises: a metal-organic framework including metal ions and an organic ligand; and a paper substrate including a fiber material. The fiber material includes a chelate fiber that has a chelate functional group capable of chelate bonding with the metal ions.

This invention discloses a method for preparing vicinal diamine derivatives, belonging to the field of pharmaceutical intermediates and organic synthesis technology. Specifically, under an inert atmosphere, an aldehyde imine, an N-aryl tertiary amine, a base, a cadmium selenide quantum dot catalyst, and a solvent are mixed and reacted under blue light irradiation for 8-24 hours. After extraction with ethyl acetate and saturated brine, the organic phase is collected, dried, the solvent is removed by vacuum distillation, and purified by column chromatography to obtain the vicinal diamine derivatives. The vicinal diamine derivatives synthesized by this invention can be used as synthetic intermediates and metal ion chelating agents in drug synthesis and fine chemical industries.

This invention provides a battery separator with ion adsorption capabilities and its preparation method, belonging to the field of new energy battery technology. It solves the problem of battery capacity decay and deterioration of cycle performance caused by ion leaching damaging the SEI film and consuming active lithium. The battery separator of this invention includes a base film and a ceramic coating on at least one surface of the base film. The raw materials for preparing the ceramic coating include porous organic framework materials, metal ion chelating agents, ceramic particulate materials, wetting agents, and binders. This invention significantly improves the dispersion ability of the slurry and shortens the dispersion time by using metal ion chelating agents instead of traditional dispersants. Simultaneously, the use of binders containing specific functional groups crosslinks with the porous organic framework materials and forms a synergistic effect with the chelating agents, thereby jointly enhancing the battery separator's ability to adsorb metal ions. This effectively solves the problem of poor stability in high-energy-density battery systems, thus significantly improving the battery's energy density and cycle life.

The invention discloses a bio-responsive high polymer material and application thereof, and belongs to the technical field of biomedical materials. The bio-responsive high-molecular material provided by the invention contains polymers shown as formulas i to viii. Wherein the polymers of the formulas i to viii have excellent fluorescence performance, aggregation-induced fluorescence enhancement performance, multicolor luminescence performance and biological response capability based on a redox reversible switch, and can be applied to sensing, counterfeiting prevention, metal ion detection, multicolor imaging of in-vivo and in-vitro cells and / or tissues and quantitative detection of in-vivo and in-vitro oxidized and / or reducing substances; active oxygen in in-vivo and in-vitro tissues and cells can be removed to achieve long-acting anti-inflammatory and anti-oxidation effects, and the compound can be used for treatment of oxidative stress related diseases and visual monitoring of the treatment process; the biological activity of enhancing tissue retention, stimulating collagen generation, repairing tissue and resisting oxidation and aging of the tissue is achieved, and the effect of filling and repairing in-vivo cells and tissue can be effectively improved; the compound also has the capability of being chelated with metal ions to form a high-molecular metal complex, and can be used for resisting tumors, resisting bacteria, filling and repairing tissues, promoting collagen generation and the like.

The present invention relates to ammonium salt solution, high iron diamine alician blue staining solution and its application, belong to the field of medical detection technology. A metal ion chelating agent is added to the ammonium salt solution of the present invention, because the metal ion chelating agent can chelate the metal ions in the solution, reduce the release of oxygen free radicals, suppress and slow down the color change reaction of N, N-dimethyl-p-phenylenediamine dihydrochloride, so as to better protect N, N-dimethyl-m-phenylenediamine dihydrochloride, improve the storage stability of ammonium salt solution, significantly improve the problem of color deepening or blackening when ammonium salt solution is stored for a long time. In addition, when the ammonium salt solution is used for high iron diamine alician blue staining solution, the staining effect of the staining solution in pathological tissue is not affected, and the background is clean and the staining specificity is good.

The application discloses a storage-stable methylglycine-N,N-diacetic acid trialkali salt composition and a preparation method and application thereof, which comprises methylglycine-N,N-diacetic acid trialkali salt, N,N-dimethylglycine metal salt, sulfite and water. The composition can be used for storing and / or stabilizing transportation of methylglycine-N,N-diacetic acid trialkali salt, improving the stability of the metal ion chelating ability of the composition product, especially the stability of the chelating ability of calcium ions and magnesium ions, and the composition product can be stably applied to the fields of daily washing, water softening and sewage treatment.

The invention relates to the technical field of nucleic acid detection, in particular to a lysis buffer solution, a kit and an extraction method for extracting free DNA of peripheral blood. The lysis buffer solution for extracting the free DNA of the peripheral blood comprises a first lysis solution and a second lysis solution, the first lysis solution takes guanidine hydrochloride as a main chaotropic agent and at least one of ammonium thiocyanate and guanidine isothiocyanate as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate and ascorbic acid; the second lysis solution takes guanidine hydrochloride as a main chaotropic agent and urea as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate, ascorbic acid and polyethylene glycol. The buffer solution of the lysate does not contain SDS (sodium dodecyl sulfate), so that the residue of SDS in an extracted product can be avoided, and the amount of extracted free DNA (deoxyribonucleic acid) is more.