The invention discloses a dyeing and finishing process of polyester-nylon composite fiber fabric. The dyeing and finishing process of the polyester-nylon composite fiber fabric comprises pretreatment, preshaping, modification treatment, dyeing, mordant removing, soaping, fixation, finishing, drying, shaping, calendering and winding. According to the dyeing and finishing process of the polyester-nylon composite fiber fabric, helicase and alhpa-amylase are adopted to conduct pretreatment, the modification treatment is conducted before dyeing, modification is conducted on performance of the polyester-nylon fabric radically, and the dyeing rate of the fabric is increased; in the dyeing process, a large amount of alkaline matter and inorganic salt are not used, a levelling agent, substitution alkali, migration inhibitor and diffusant are added, the level-dyeing property is improved, natural dyestuff is used, therefore, environmental pollution is avoided, and requirements of green environmental protection are met; self-cleaning treatment is added in finishing, and the self-cleaning capacity, crease resistant property and fastness to washing of the fabric are improved; the whole process is environmentally friendly and free of pollution.