The present application relates to the technical field of polypeptide compounds, and particularly relates to a whitening polypeptide compound, a preparation method and application thereof, wherein the whitening polypeptide compound is obtained by free arrangement and combination of
tryptophan,
tyrosine and beta-
alanine through an
amide bond. The whitening polypeptide compound provided by the present application has excellent whitening activity, and is verified by B16-F10 cells induced by alpha-MSH. The whitening polypeptide compound can efficiently inhibit
skin melanin synthesis, and has the effects of whitening and
freckle lightening. Meanwhile, the whitening polypeptide compound has high whitening activity, good mild safety, a simple preparation process, high synthesis efficiency, low cost, strong stability in a cosmetic
system, good
skin permeability, can simultaneously realize efficient whitening,
freckle lightening and
skin barrier repair, can be widely applied to whitening
cosmetics to prepare various dosage forms, improve product whitening durability and stability, has high application value, and meets the demand of the
cosmetic industry for high-performance mild whitening active ingredients.