Method for transforming pentosan and dietary fiber by wheat bran enzyme engineering method
A wheat bran and dietary fiber technology, applied in the field of food processing, can solve the problems of low added value, etc., and achieve the effects of increasing dietary fiber content, increasing economic income, and large flexibility
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[0036]The wheat bran is first processed by a bran breaker, and then sieved through 80 mesh to remove the sticky flour in the bran, and then crushed through a 40 mesh sieve. After crushing, the bran is adjusted to pH 5 with dilute hydrochloric acid at a ratio of material to water of 1:6, and cell wall decomposing enzymes (xylanase, cellulase or pentosanase, 40u / 100mL) are added, and enzymatic hydrolysis at 45°C for 1 hour , Centrifugation, and then use an extruder to treat the wheat bran. On the one hand, the enzyme is inactivated, and on the other hand, the structure of the wheat bran is further loosened to facilitate subsequent extraction and separation. The expanded wheat bran is then crushed to 40 meshes. screen. Weigh 50g of crushed bran sample into a reactor equipped with a stirring device, add 250mL of water, 120u of cellulase, stir in a water bath at 45℃ for 1h, and then use saturated Ca(OH) 2 The solution was adjusted to pH=10 and stirred at 50°C for 2h; the reaction solut...
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