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This invention discloses a high-functionality defatted rice bran, its preparation method, and its applications. The method includes: stabilizing fresh rice bran with saturated steam to inactivate lipase, obtaining stabilized rice bran; then using the stabilized rice bran as raw material, extracting and defatting it with supercritical CO2 to separate rice bran oil and high-functionality defatted rice bran. Preferably, the saturated steam treatment temperature is 115-125℃, and the time is 15-25 min; the supercritical CO2 extraction temperature is 45-55℃, the pressure is 25-35 MPa, and the time is 1.5-2.5 h. The defatted rice bran prepared by this invention has a fat content of less than 1.5%, a water-holding capacity of more than 3.0 g / g, an oil-holding capacity of more than 2.4 g / g, and an expansion potential of more than 5.8 g / g. This method is green and environmentally friendly, significantly improving the functional properties of defatted rice bran. The resulting product can be used as a high-dietary-fiber ingredient in baked goods, showing good application prospects.