204 results about "Bacillariophytes" patented technology

The invention provides a biological bacterial fertilizer for preventing and treating moss in a water body and a preparation method thereof. The biological bacterial fertilizer is prepared from sodium humate, amino acid raw powder, Bacillus subtilis and sodium silicate. The sodium humate dissolved in water can turn water into light black or black and perform the function of shielding sunlight, so that the moss or mud cake on the tank bottom dies due to loss of lighting; the free amino acids and silicic acid can quickly promote the growth of the beneficial algae, thereby quickly cultivating the algae and enriching the water; and the Bacillus subtilis in the biological bacterial fertilizer can generate multiple enzymatic systems to decompose and utilize organic matters in the moss, mud cake, residual bait and manure in the culture water body, provides nutrients for algae growth, forms favorable diatom water color and lowers the transparency of the water body. The biological bacterial fertilizer has multiple biological water enrichment functions of killing the moss, decomposing the organic matters and quickly cultivating the beneficial algae. The biological bacterial fertilizer has the advantages of no environmental pollution, no public nuisance, low cost, wide application range and the like, is biodegradable and is simple to operate.

The invention relates to a preparation method for a diatomaceous earth-supported nanometer carbon composite adsorption material, and belongs to the field of nonmetallic mineral material deep-processing and environmental engineering. The surface and pore passages of diatomaceous earth are taken as micro-reaction areas, glucose or starch is taken as a carbon source, and generated nanometer carbon particles are uniformly supported to the surface and the pore passages of diatomaceous earth to obtain the efficient nanometer carbon composite adsorption material with diatomaceous earth as a supporter by a hydrothermal synthesis method. According to the method, a process is simple, the nanometer carbon particles are uniformly and densely distributed on the surface and in the pore passages of diatomaceous earth, the adsorption of nanometer carbon and diatomaceous earth to pollutants in water is greatly improved, and nanometer carbon and diatomaceous earth have high potential application value in the field of wastewater treatment.

The invention discloses a bait-food for promoting the growth speed of sea hedgehog living, which consists of ulva immature spore attached to an attaching base and benthic diatom. The quantity ratio between the ulva immature spore and the benthic diatom is 1 : 40-80. The preparation method has the following specific steps: natane of the ulva is put in a sea pool which the attaching base is placed. The sea pool is cultured until the attaching base has attachments with a density of 1,500-2,500 / mm<2> and the attaching base is then poured into a new pool; 20g / m<3> of nutritive salt NaNO3 and 2g / m<3> of KH2PO4 are added into the sea water in the new pool; illumination intensity is 3800-4700lux and water temperature is 18-23 DEG C; pH is 31-33.4 and the attachments are cultured in an air inflation way until the ulva immature spore is produced; the benthic diatom is gathered on the attaching base and the quantity ratio between the ulva immature spore and the benthic diatom is 1 : 2-6; 30g / m<3> of NaNO3, 3.5g / m<3> of KH2PO4 and 0.2g / m<3> of NaSiO3 are added into the sea water and the illumination intensity is 2,800-3,600lux; the ulva immature spore and the benthic diatom are cultured until the quantity ratio thereof is 1 : 40-80.

The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and / or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and / or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceæ such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and / or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and / or vectors. A further part of the invention relates to oils, lipids and / or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

The invention discloses a device and a method for cultivating diatom. The device for cultivating the diatom comprises a cultivation container, a diatom attaching plate and inflation pipes. The cultivation container is used for accommodating cultivation media, the diatom attaching plate is arranged inside the cultivation container, and the inflation pipes are arranged inside the cultivation container and are used for blowing air to the diatom attaching plate. The method for cultivating the diatom includes a diatom seed cultivation step, a mass cultivation step, a harvesting step and a low-temperature stimulation step. According to the technical scheme, the device and the method have the advantages that problems of strict conditions, high energy consumption, vulnerability to pollution and low cell density of existing micro-diatom liquid cultivation can be solved by the aid of the device and the method; the device is simple in structure, the diatom biomass yield can be effectively increased by the device and the method, micro-diatom can be cultivated without strict conditions, the device and the method are low in energy consumption, pollution can be prevented, and high-density diatom cells can be generated.

The invention discloses a novel environment-friendly wall material. The novel environment-friendly wall material is prepared from, by weight, 25-28 parts of diatomite, 15-20 parts of ground calcium carbonate, 15-20 parts of quartz sand, 8-10 parts of attapulgite, 5-8 parts of sepiolite, 5-8 parts of zeolite powder, 8-10 parts of sierozem powder, 0.5-1 part of redispersible rubber powder, 0.1-0.3 part of lignocelluloses, 0.01-0.03 part of fragrant plants and 0.1-1 part of pigments. The attapulgite and the fragrant plants are jointly roasted at a low temperature to realize complete fragrance adsorption, fragrance of the prepared wall material lasts for half a year, and extracted essential oil can be used for fragrance replenishment to prolong fragrance duration. The diatom ooze wall material with a healthcare efficacy endows a room with natural fragrance by making the best of fragrance and antibacterial effects of the fragrant plants while providing colors, thereby giving residents full experience. In addition, contents of raw materials are more reasonable, and besides characteristics of existing diatom ooze, the prepared wall material is stronger in texture and easier for construction.

The invention belongs to the field of pesticide sustained-release agents and discloses an adsorptive pesticide sustained-release agent preparation method. The method includes: preparing purified diatomite from diatomite, modifying lignin to obtain epoxidized lignin, preparing a modifier from the epoxidized lignin, and mixing the modifier with the purified diatomite to realize modification reaction to obtain an adsorptive pesticide sustained-release agent. By the modifier prepared from the lignin, adsorption performance of bentonite can be improved, and various active functional groups of the modifier have a certain adsorption effect on organic matters. By the prepared pesticide sustained-release agent, pesticide loading capacity is improved, pesticide efficacy duration is prolonged, pesticide utilization rate is increased, and environment pollution caused by pesticides is relieved.

The invention discloses diatom mud and a preparation method thereof. The diatom mud comprises the following raw materials by weight parts: 1,500-3,000 parts of diatomite, 0.1-0.5 part of mica, 0.1-0.2 part of cellulose, 1,000-2,000 parts of talcum powder, 0.1-0.2 part of titanium platinum, 0.1-0.5 part of shadowless glue and the balance of water. The porous diatom mud is prepared from the materials such as the diatomite, the mica and the like; indoor harmful substances, especially chemical substances such as formaldehyde and the like are adsorbed through the porous diatom mud; the degradation rate and the removal rate of the indoor harmful substances are increased; the removal rate of indoor formaldehyde by the diatom mud as a coating material is more than 80%; moreover, the situations of cracking and the like of the diatom mud applied to a wall can not be caused, and then the interior decoration is more attractive.

The invention provides a nano diatom water purifying agent and a preparation method thereof. The nano diatom water purifying agent provided by the invention is mainly prepared by mixing diatomite andaluminum-containing and iron-containing minerals. According to the invention, two modification means of roasting and acid leaching are carried out on a natural diatom ore product, so that the pore size, the specific surface area and the adsorption capacity of the diatomite are increased; and by adding the nano diatom composite water purifying agent in a sewage treatment process, ultrafine particlesubstances are adsorbed to the surface of the diatomite by using the huge specific surface area and the strong adsorption force of the diatomite, and the flocculation effect is combined, such that the pollution in sewage can be rapidly flocculated and precipitated so as to substantially improve pollutants, inorganic substances and the like and improve the biodegradability of the sewage.

The invention provides a biological agent for preventing and treating mosses in a water body and a preparation method of the biological agent. The biological agent is prepared from the following raw materials: food-grade brilliant blue, bacillus subtilis, yeast and sodium silicate. The brilliant blue in the biological agent provided by the invention is dissolved into water to enable the water to become light blue, so that the sunlight is shielded, and mosses and mud cakes at the bottom of a pool are dead of losing illumination; bacillus subtilis and yeast in the biological agent generate various enzyme systems to decompose and utilize the mosses, mud cakes and residual feeds in the water body and organic matters in faeces and provide nutrients for the growth of algae, and sodium silicate induces useful algae and diatoms to rapidly growth to form a preponderant algae species. The biological agent plays various biological water regulating roles in killing the mosses, decomposing the organic matters and promoting the growth of the useful algae. The biological agent can be used for preventing and treating mosses in a culture pond, a landscape water body and offshore seawater and has the advantages of no environment pollution and public hazards, biodegradablity, low dosage, simplicity in operation, wide application range, long duration time and the like.

The invention discloses a synergistic bio-organic fertilizer and a preparation method thereof. The invention relates to the technical field of bio-organic fertilizer production. The synergistic bio-organic fertilizer is prepared by mixing and fermenting the following raw materials in percentage by weight: 50-70% of livestock and poultry manure, 10-21.95% of crop straws, 5-15% of soybean meal powder, 0.05% of an organic material decomposition agent, 1-5% of diatom shale, 0.5-2% of activated carbon, 2-10% of turf, 0.5-3% of bacillus subtilis and 0.5-2% of paenibacillus mucilaginosus. The bio-organic fertilizer disclosed by the invention meets the bio-organic fertilizer detection standard of the Ministry of Agriculture, is high in fertilizer efficiency and short in fermentation period, does not cause secondary pollution to the environment, and is suitable for industrial production. The problems of long fermentation period, low fertilizer efficiency of organic fertilizer products and soil hardening caused by long-term use of chemical fertilizers in the organic fertilizer production process can be solved, and large-scale application can be realized.

The invention discloses a nutrition formula used for promoting rapid growth of benthic naviculaceae. The nutrition formula is characterized in that the nutrition formula comprises macroelements, microelements and vitamins, wherein the macroelements comprise nitrogen, phosphorus and silicon, the microelements comprise iron, zinc, copper, manganese, molybdenum and cobalt, and the vitamins comprise vitamin B1, vitamin B12 and vitamin H. According to growth characteristics of requirements of the benthic naviculaceae to different nutritive salts, the formula can achieve an objective of promoting rapid growth of purebred benthic diatom cultures. The nutrition formula has reasonable nutrition collocation, and is capable of effectively promoting rapid division growth of the naviculaceae, promoting absorption and utilization of a variety of nutrients, accelerating metabolism of naviculaceae cells and improving the production quantity.

The invention provides a termite attracticide, which comprises the following raw materials: paper, chlorfenapyr, ivermectin, asparaginic acid, chitosan, saccharose, pollen pini and diatom powder, and further comprises tea tree root powder and bismuth oxyhalide. The invention further discloses a preparation method of the termite attracticide. The method comprises the following steps: mixing and spraying raw materials on paper; preparing into the required shape; and drying to obtain the termite attracticide. By virtue of the formula, the dosage of a chemical agent is greatly reduced; and the security threats to the environment and human are reduced.

The invention relates to an ecological dredging recycling method. The method is realized in the following steps: sediment is pretreated, sediment organic liquid fertilizer is fermented, a bacterial sediment liquid is added to a lake, reservoir or river to be used as a microbial strain, a nutrient solution or a concentrated nutrient solution passes through an aeroponic cultivation system, a residual plant absorption liquid is obtained through absorption of nutrient substances by plants, bacterial sediment is flavored by a flavoring agent, mycoprotein feed is prepared, and an alga liquid is obtained through alga culture of the residual plant absorption liquid; an alga culture container is made of a high-transmittance high-polymer hard material; algae comprise green algae and diatom; the culture period lasts 48-72 h; the illumination intensity is 3,000-7,000 LX, and the illumination time lasts 48-72 h; the obtained mycoprotein and the alga liquid enter a high-efficiency bioreactor via a feed adding system and a pipeline, residual feed and excrement of all aquatic organisms in the high-efficiency bioreactor are collected and dewatered and then enter an organic fertilizer fermentation system for fermentation recycle, and aquiculture water is directly discharged into a water body after being disinfected by a biological purification system in the high-efficiency bioreactor.

The invention discloses a utility model efficient purification diatom mud wall material as well as a preparation method and a construction method thereof, and belongs to the field of wall material preparation. The utility model efficient purification diatom mud wall material is prepared from the following components: 10 to 30 parts of diatomite, 30 to 60 parts of an inorganic alkalescent porous material, 20 to 70 parts of an adhesive, 1 to 10 parts of active clay, 5 to 20 parts of pigment, 5 to 10 parts of an auxiliary material, 1 to 10 parts of a nano functional material and 10 to 20 parts of glucoside plant extract. According to the utility model efficient purification diatom mud wall material disclosed by the invention, the inorganic alkalescent material is used as a main base material, and the glucoside plant extract is used as a core, so that a better formaldehyde removing effect is achieved through a synergistic effect with the nano functional material; by utilizing the adsorption of zeolite powder and the diatomite, and under the coexistence of the glucoside plant extract and the nano functional material, adsorbed formaldehyde can be effectively decomposed, and the function of purifying indoor air can be achieved. A preparation method and a construction technology are simple, and large-scale production is convenient.

The invention discloses an induced type hydrobios collecting and water purifying device. The induced type hydrobios collecting and water purifying device comprises an induced type artificial fish spawning nest and a bottom biology collecting device which are connected integrally through an anchoring rope, wherein the induced type artificial fish spawning nest comprises an upper module, a lower module, a consolidation diatom attaching plate; the upper module comprises floating island modules, aquatic plates and an upper frame; the lower module comprises a lower frame, viscid egg attaching modules and viscid egg attaching matrix; the bottom biology collecting device comprises an upper housing and a lower housing, wherein the upper housing is a curved surface housing in the shape of a shell,and a plurality of circular holes are uniformly formed in the upper housing; and a gravel layer, fixed pebbles and movable pebbles are sequentially stuffed in the lower housing from bottom to top, anddiversion blades are respectively arranged at the rear end of the upper housing and the rear end of the lower housing. The device can realize induced collection of adherence fish eggs, adherence sunkfish eggs, consolidation diatom, and demersal animals, and besides, has the function of purifying water bodies.

Various aspects provide for extracting siliceous particles. Siliceous particles may include or be derived from diatoms. Certain embodiments provide for segregating suspensions into two or more segregation products. In some cases, a first product includes siliceous particles, and a second product may include hydrophobic species. Certain aspects provide for extracting non-siliceous biomass (e.g., lipids).

The invention discloses a bostrychus sinensis feeding method and an acoustic domestication feeding system thereof. The method is characterized in that excellent diatom is inoculated after water changing, chaetoceros is selected as the diatom, the feed feeding is decreased progressively according to the levels of 8%, 7%, 6%, 5% and 4% of the total weight of fish, each level is fed for 3 days, 15 days serve as a period, after 15 days, 100% water change is carried out, a fishing pond is cleaned, weighing is carried out, the above operation is repeated, an acoustic domestication feeding system isadopted for feeding during feeding, the acoustic domestication feeding system comprises a support frame, and a hydrophone and a camera which are positioned in a culture pond, four feeding machines, aloudspeaker and a controller are arranged on the support frame, and the feeding machines, the loudspeaker, the hydrophone and the camera are all electrically connected with the controller. The methodhas the functions of improving the feeding efficiency, reducing the feed loss, adjusting the pH value of the water body and inhibiting the growth of bacteria, so that the fatality rate of the bostrychus sinensis is reduced, and the survival rate of the bostrychus sinensis is increased.

The invention relates to a growth-beneficial algae growth accelerant and a preparation method and application thereof. The preparation method of the growth-beneficial algae growth accelerant comprisesthe following steps of mixing aluminum oxide, silicon source, a catalyst and a first solvent by a sol-gel method, so as to obtain the aluminum oxide-modified nanometer silicon dioxide powder; mixingthe aluminum oxide-modified nanometer silicon dioxide powder, water-soluble organic iron and a second solvent to react, so as to obtain the growth-beneficial algae growth accelerant. The growth-beneficial algae growth accelerant is used for well accelerating the growth of diatom.

The invention belongs to the field of environmental protection, and particularly relates to a preparation method for a diatomite membrane and determination of a method for treating a eutrophic water body. The diatomite membrane is a microfiltration ceramic film taking diatomite as a raw material, and comprises the following components in percentage by weight: 65-94% of diatomite, 0.5-8% of natural zeolite and 5-30% of starch. Before the eutrophic water body is treated by utilizing the diatomite membrane, a coagulant is added firstly to pre-treat the water body, so that contaminants such as algae, N, P and the like in the water body can be effectively removed. The coagulation diatomite membrane has the characteristics of high treatment efficiency, no secondary pollution and no damage to algae cell bodies.

The invention provides soil microalgae of a mining area, a separation and purification method for the soil microalgae of the mining area and application of the soil microalgae of the mining area. Thesoil microalgae of the mining area are separated and purified from biological crusts of surfaces of tailings and comprise one or more selected from Chlorella, Cylindrotheca, Diatom, Nitzschia, Schizochytrium, Dunaliella, Scenedesmus, Nannochloropsis oculata, Chlamydomonas, Tetraselmis and Eudovina. The soil microalgae of the mining area, provided by the invention, are separated and purified from the biological crusts of the surfaces of the tailings; the obtained microalgae are relatively extensive in variety; and the obtained microalgae have relatively good adsorption and absorption capabilityto contaminants such as heavy metals and phosphorus in contaminated soil and water and then can effectively treat the contaminants such as the heavy metals and phosphorus in the contaminated soil andwater, so that the treatment cost of the contaminants such as the heavy metals and phosphorus in the contaminated soil and water is reduced greatly.

The invention provides a water treatment agent containing high-porous diatom, which is composed of the high-porous diatom, a flocculating agent and an assistant agent according to the mass ratio of 60-70:20-30:1-10. The water treatment agent has the effects of adsorption and flocculation when being added during wastewater treatment. The water treatment agent also is combined with microorganisms in a bio-pool to serve as a bio-carrier, thus greatly improving treatment efficiency. The water treatment agent can be used for treatment of various industrial and living wastewaters, has good treatment effect, low cost, small occupied area and reduced sludge amount, and can treat the wastewater fundamentally.

The embodiments of the invention disclose water purification powder for killing euglena. The water purification powder is prepared from the following components in parts by mass: 1-10 parts of zeolitepowder, 51-90 parts of polyaluminum chloride, 0.01-1.5 parts of an adsorbent, 0.2-5.6 parts of a water antidote, 0.2-4.5 parts of sodium percarbonate powder and 0.01-1.5 parts of a disinfectant. Theembodiments of the invention also provide a preparation technology of the water purification powder for killing the euglena. The water purification powder for killing the euglena has selectivity for algae, only kills the euglena and has no effect on other algae, is beneficial to breeding of beneficial algae such as green alga, diatom and chlorella, and is capable of precipitating and purifying water quality.

The invention discloses a preparation method of a diatom ooze special for walls and belongs to the technical field of building materials. The method comprises performing ultrasonic dispersion, filtering, carbonization and cooling onto diatomite and peach gum liquid to obtain carbonized materials; stirring and mixing the carbonized materials, nano iron powder, boric oxide, sodium carbonate and calcium carbonate, performing decompressed distillation, high-temperature reaction and cooling to obtain preprocessed diatomite; mixing the preprocessed diatomite, biogas slurry, blue-green algae and water for fermentation, and performing filtering, washing and drying processes to obtain dried, fermented and filtered cakes; heating, stirring and mixing the dried, fermented and filtered cakes with potassium permanganate solution, adjusting the pH, and performing soaking, filtering and washing processes to obtain modified diatomite; mixing glycol, the modified diatomite, pigments and fillers, quartzsand, silicon powder, redispersible powder, water, ethylene-vinyl acetate copolymer and silane coupling agent, and then adding in and stirring sulfuric acid and hydrogen peroxide mixture solution formixing to obtain the diatom ooze special for walls. The diatom ooze special for walls is good in bonding strength.

The invention discloses a method for extracting pure diatom shells from marine unicellular diatom. The method comprises the following steps: (1) preparing algae mud; (2) treating algae mud by an acid-heat method; and (3) washing and drying. Compared with the prior art, the method has the advantages that the operation process is simple and feasible; the completeness is good, and the diatom shell treated by using the mixed acid solution of sulfuric acid and nitric acid is complete in structure and clear in pore; the cleanliness is high, organic matter and harmful solvent residues do not exist on the surfaces of the diatom shells, and the purity is high; the invention provides a simple and efficient method for extracting and separating high-purity diatom shells from marine diatom and other unicellular organisms containing the diatom shells, and further research of a factory-like large-scale diatom shell extraction and separation process is facilitated.

The invention relates to a riverway sludge repairing material and a preparation method thereof. The riverway sludge repairing material is prepared from, by weight, 15-25 parts of diatom ooze, 3-5 parts of disodium edetate dihydrate, 4-8 parts of calcium peroxide, 25-35 parts of carrier base material, 9-13 parts of compound flora, 4-6 parts of chitin, 5-7 parts of polyacrylamide, 10-15 parts of humus, 3-4 parts of sorbic acid and 100-120 parts of deionized water. The riverway sludge repairing material is easy to obtain, a fixed layer is formed on the surface of bottom sediment through biological breeding, the relation between a water source and sludge is effectively blocked, effective nutrients in the sludge are absorbed and released into the water source, heavy metal ions, sulfide and phosphide in the water source are effectively removed, the dissolve oxygen concentration in the water source is improved, the stink is eliminated, water quality is improved, aquatic plant growth is promoted, and restoration and development of a riverway ecosystem are accelerated.

The present invention is generally directed to processes that integrate CO2-producing conversions of hydrocarbonaceous assets with biofuels processes that utilize CO2 in photosynthesis. In some embodiments, such processes involve the absorption of CO2 in an absorption liquid. In some such embodiments, such absorption is carried out in an absorption tower. In some other such embodiments, there is a subsequent desorption of the CO2. Generally, at least some of the CO2 captured by the absorption liquid is used to grow microbes or diatom species.

The invention relates to an epiphytic diatom culture and collection device. The culture and collection device comprises an on-water floating body, a suspending cage, culture medium, a stainless steel wire rope, an anchor device, a porcelain dish, a brush, a narrow-mouthed bottle, formaldehyde reagent and the like, wherein the suspending cage is installed with a water temperature and flow rate integrated intelligent remote sensor; a signboard is placed at the top of the on-water floating body, the floating body is installed with a signal acquisition and transmitting device and a power supply, and water temperature and flow rate are monitored through wireless remote monitoring and are transmitted to a data center through wireless transmission. The diatom culture and collection device disclosed by the invention can flexibly and conveniently realize the culture and collection of epiphytic diatom in rivers and lakes which lack for qualified sampling medium, and can acquire the water temperature and flow rate of the region on line according to the needs.

The invention provides a microbial agent and a preparation method thereof, and relates to the technical field of biology. The present invention provides a microbial agent and a preparation method thereof. The microbial agent is prepared from the following raw materials in parts by weight: 65-90 parts of lactobacillus casei bacterial liquid, 35-45 parts of rhodospirillum rubrum bacterial liquid, 35-45 parts of bacillus subtilis bacterial liquid, 5-9 parts of red algae powder, 10-15 parts of glucose, 8-12 parts of skimmed milk, 6-9 parts of serum, 4-6 parts of guar gum, 7-10 parts of starch, 6-7 parts of sodium bicarbonate, 4-5 parts of citric acid, 4-8 parts of vermiculite powder and 1-2 parts of diatom element. The lactobacillus casei is matched with rhodospirillum rubrum and bacillus subtilis to prepare the microbial agent, the digestive ability of aquatic animals can be improved, harmful germs can be killed, the water environment can be purified, the adaptability of the microbial agent to high-altitude areas is improved by screening low-pressure-resistant flora in a low-pressure environment and then propagating the low-pressure-resistant flora, and the microbial agent is worthy of being vigorously popularized.

The invention belongs to the industry of aquaculture and particularly relates to a benthic diatom culture method used for culturing breeding feed. The method is characterized by comprising the following steps: inoculating navicula tenera obtained by separation and purification and nitzchia palea to a liquid F culture medium in certain cell density, putting a circulating pump in the liquid F culture medium, and performing intermittent circulation, namely performing circulation for 10 minutes at intervals of every 2 hours, wherein the formula of the improved F culture medium comprises 149.6mg ofNaNO3, 8.8mg of NaH2PO4, 3.9mg of FeC6H5O7.5H2O, 1mL of trace element solution mother liquor, 1mL of vitamin solution mother liquor, 1000mL of pure water, vitamin mother liquor, 100mg of vitamin B1,0.5mg of vitamin B12 and 500mL of pure water; culturing benthic diatom in a 5000mL conical flask; and normally boiling seawater by adopting the improved F culture medium, wherein the salinity is 31, the illumination intensity is 50mu mol E.m<-2>s<-1>, the illumination period is 16L:8D, and the temperature is 20 DEG C. The benthic diatom culture method is simple, convenient and fast in operation, low in cost and suitable for popularization and application.