132 results about "Bacillariophytes" patented technology

The invention relates to a bacteria-algae composite adsorption module wetland substrate filler as well as a preparation method and application thereof, and belongs to the technical field of biology. The bacteria-algae composite adsorption module wetland substrate filler is prepared by the following steps: preparing an adsorption module, fungicide microspheres and a diatom biological membrane layer, placing the diatom biological membrane layer on the upper layer of the fungicide microspheres to form a diatom fungicide composite layer, and placing the diatom fungicide composite layer on the upper layer of the adsorption module. The bacteria-algae composite adsorption module wetland substrate filler disclosed by the invention can effectively remove pollutants such as nitrogen, phosphorus, lead, cadmium and the like in sewage by independently using the bacteria-algae composite adsorption module wetland substrate filler, effectively reduces the chemical oxygen demand (COD) of the sewage, and simplifies the hierarchical design of an artificial wetland; and the regeneration capacity is high, the replacement period is prolonged, and the operation and maintenance cost is reduced.

The invention particularly relates to a method for cultivating biological bait in an outdoor pond. According to the method, after an outdoor pond is disinfected and cleaned, a biological fertilizer source and various beneficial bacteria combination are used in a combined mode, cryptococcus ovale, composite diatom and chlorella are inoculated, a stable pond bacterium-algae system is constructed, and various cultivated microalgae are used for strengthening zooplanktons of rotifers, cladocerans and copepods in the bait pond. The breeding period of the biological bait is shortened, the survival rate of the biological bait is increased, the yield of the biological bait is increased, meanwhile, the nutritional value of the biological bait is enhanced, and opening breeding of the fry is facilitated. The breeding method is low in cost and suitable for various weathers, and sufficient bait guarantee is provided for large-scale breeding of fish fries.

The invention discloses a phenyl ether bimetal organic framework antifouling material, a preparation method and application, and belongs to the technical field of marine antifouling materials. The preparation method comprises the following steps: 1) dissolving a carboxyl-containing phenyl ether organic ligand, a zinc salt and a lanthanide metal salt in a polar organic solvent, and carrying out a coordination reaction in a closed reaction container, so that zinc and lanthanide metal ions and carboxyl form coordinate bonds, and bridging the metal ions to form tetra-metal clusters; washing and drying crystals obtained after the reaction to obtain the phenyl ether-based bimetal organic framework; and 2) grinding the phenyl ether-based bimetal organic framework, uniformly dispersing the ground phenyl ether-based bimetal organic framework into the waterborne polyurethane resin, and fully mixing to obtain the phenyl ether-based bimetal organic framework antifouling material. The invention also provides an application of the antifouling material in marine antifouling and anticorrosion. The prepared antifouling material is low in synthesis cost and difficulty and stable in property, and has a remarkable inhibition effect on biological sedimentation of diatom, barnacle and the like.

The invention belongs to the technical field of lake and reservoir water source risk prediction, and provides a lake and reservoir water source algae bloom risk early warning system based on a 16S / 18SrRNA gene expression quantity threshold, and the method comprises the following steps: S1, collecting a sample from a lake and reservoir water source surface layer water body; s2, using 7-gate water bloom algae specific primers for blue-green algae, green algae, diatom, euglena, dinoflagellate, chrysophyta and cryptoalga; s3, based on the qPCR standard curve, calculating the copy number of the 16SrRNA / 18SrRNA gene of the seven water bloom algae; s4, when the gene expression quantity of a certain algal bloom algae continuously reaches 106-7 copy number / mL for 7-12 days, determining that the algal bloom algae is in a window phase; according to the threshold value of the gene expression quantity of the water bloom algae 16SrRNA / 18SrRNA, the window period of water bloom algae cells can be accurately recognized, so that early warning of the algae bloom risk in the lake and reservoir water source is achieved, and early warning of algae bloom outbreak in the lake and reservoir water source is achieved one week or above in advance.

The invention discloses a lake and reservoir dominant algae community structure prediction method and system based on machine learning and a storage medium, and relates to the technical field of water environment monitoring and ecological prediction. Comprising the following steps that multi-source heterogeneous spatio-temporal data are obtained and fused, and the multi-source heterogeneous spatio-temporal data are historical time sequence data of a target water area; generating a feature vector from the fused multi-source heterogeneous spatio-temporal data; constructing a multi-output regression machine learning model, and inputting the feature vector into the multi-output regression machine learning model for training to obtain a multi-output prediction model; and inputting real-time monitoring data into the multi-output prediction model to generate a prediction result of a future dominant algae community structure. According to the method, whether cyanobacterial bloom or diatom bloom exists in the future can be clearly early warned, so that a manager can take targeted measures, for example, a specific algicide or ultrasonic equipment is used for the cyanobacteria, blind pesticide application is avoided, and the cost and the secondary pollution risk are reduced.

The invention discloses a method for directionally culturing diatom by using culture tail water and application, and belongs to the crossing field of aquaculture and wastewater treatment.The method for directionally culturing diatom in the culture tail water comprises the following steps: S1, precipitation: discharging the culture tail water into a precipitation tank to precipitate silt and large particles; s2, sterilization and algae removal: ultraviolet rays are adopted to irradiate the culture tail water to kill bacteria and original algae in the culture tail water; s3, adjusting the nutrition structure: adjusting the total salinity of the culture tail water to 20-30% o, the silicate content to 500-1000 [mu] mol / L, the phosphate content to 0.6-0.9 mg / L and the ammonium salt content to 4-6 mg / L; s4, algae species and bacteria inoculation and culture: diatom and diatom symbiotic bacteria are inoculated into culture tail water. By using the method provided by the invention, the directional regulation and control of the microalgae community in the culture tail water can be realized, and the removal rate of nutritive salts in the culture tail water and the resource recovery rate are greatly improved. Meanwhile, by-product shellfish organisms can appear on the market through subsequent culture, the economic benefit is improved, and the purification cost is reduced.

The utility model embodiment discloses a diatom culture device for aquaculture, including a transparent circular culture box and an L-shaped ventilation pipe, a collection tank is provided on the transparent bottom plate of the circular culture box, a drain valve connected to one end of the collection tank is installed at the bottom of the transparent bottom plate; a plurality of transparent attachment plates are mounted on the vertical part of the ventilation pipe, and a scraper is fixedly connected to one side of the lower end of the ventilation pipe, the width of the scraper is greater than the width of the collection tank, and when the scraper covers the collection tank, a sewage channel is formed inside the collection tank. The present application can greatly improve the illumination rate of diatoms and the growth rate of diatoms through the transparent circular culture box, the transparent bottom plate and the reflective mirror; a sewage channel is formed through the combination of the scraper and the collection tank, and when the scraper pushes the diatoms at the bottom into the collection tank, the drain valve is opened, and the bottom can be cleaned when the water is not completely drained, which is very convenient to operate and saves time and effort.

The invention provides a method for culturing beneficial algae by resource utilization of cyanobacterial blooms, which comprises the following steps: 1, obtaining cyanobacterial (microcystis) blooms, and concentrating the cyanobacterial (microcystis) blooms until the concentration of chlorophyll a is 80-150mg / L; 2, carrying out continuous hydrodynamic disturbance on the high-concentration cyanobacterial bloom, wherein the disturbance intensity is suitable for preventing the cyanobacterial bloom from blackening and stinking; 3, continuously disturbing for 20-60 days to obtain cyanobacterial bloom which turns light yellow green or white; 4, tap water is used for diluting cyanobacterial blooms with changing colors, the concentration during dilution is 0.5-2.3 mL / cm < 2 >, and beneficial algae such as diatom and green algae are inoculated for culture under the conditions of normal temperature and weak light; and 5, when the culture is performed for 12-30 days, the growth density of the beneficial algae reaches the highest peak, and the cell density of the beneficial algae can reach up to 2.5 * 10 < 6 >-5.6 * 10 < 7 > cells / cm < 2 >. According to the method, algal toxin can be removed, beneficial algae such as diatom and green algae can be cultured through resource utilization of the cyanobacterial bloom, and a new way is provided for control and resource utilization of the cyanobacterial bloom.

The present invention discloses a phenyl ether-based bimetallic organic framework antifouling material, its preparation method, and its application, belonging to the technical field of marine antifouling materials. The preparation method is as follows: 1) A phenyl ether organic ligand containing a carboxyl group, a zinc salt, and a lanthanide metal salt are dissolved in a polar organic solvent and placed in a sealed reaction vessel for a coordination reaction, whereby the zinc and lanthanide metal ions form coordination bonds with the carboxyl groups, bridging the metal ions to form a tetrametallic cluster; the resulting crystals are washed and dried to obtain the phenyl ether-based bimetallic organic framework; 2) the phenyl ether-based bimetallic organic framework is ground and uniformly dispersed in an aqueous polyurethane resin, followed by thorough mixing to obtain the phenyl ether-based bimetallic organic framework antifouling material. The present invention also provides the application of the antifouling material in marine antifouling and anticorrosion applications. The antifouling material prepared by the present invention has low synthesis cost and difficulty, stable properties, and significant inhibitory effect on the settlement of organisms such as diatoms and barnacles.

The invention relates to a jellyfish disaster early warning method, jellyfish disaster early warning equipment and a medium. The method comprises the following steps: acquiring the copepod dominance of a target water area on a date T and the diatom abundance of a date T-# imgabs0 #; if the copepod dominance is equal to or greater than a first threshold value and the diatom abundance is equal to or greater than a second threshold value, determining a jellyfish outbreak risk index of the target water area on a date T + # imgabs1 # according to the copepod dominance and the diatom abundance; otherwise, inputting the copepod dominance of the target water area on the date T, the diatom abundance of the date T-# imgabs2 # and the water quality parameter of the date T into a target prediction model corresponding to the target water area, and outputting a jellyfish outbreak risk index of the target water area on the date T + # imgabs3 #; and determining a jellyfish outbreak risk level of the target water area according to the jellyfish outbreak risk index. According to the scheme, the jellyfish outbreak risk index can be predicted in advance by combining the copepod dominance degree of the date T and the diatom abundance of the date T-# imgabs4 #.

The invention discloses a preparation method and application of an anti-fouling coating system capable of preventing biological adhesion, the anti-fouling coating system capable of preventing biological adhesion comprises a primer and a topcoat, and the primer comprises a first component and a second component. According to the preparation method and application of the anti-fouling coating system capable of preventing biological adhesion, the anti-traditional thinking is used for developing an anti-fouling coating, the surface coating is used for developing a nano composite particle coating, a high-surface-energy nano material is firstly used as a basis, a dark catalytic electrolysis technology is matched for preparation, the characteristic of high surface energy of the nano material is adopted, and the anti-fouling coating system capable of preventing biological adhesion is prepared. Energy is absorbed, stored, converted and released through the composite nano-material, the nano-material breaks walls of marine microorganisms and a generated electric field effectively prevents generation of a biological membrane, then growth of marine bacteria, shells and benthic diatom is effectively inhibited, meanwhile, two-dimensional nano-graphene is adopted to replace traditional graphene oxide for modification, the overall engineering is simple, and the cost is low. More atoms are distributed on the surface, and the coating surface has higher energy.

The present invention discloses a method for constructing acid-resistant engineered algae, belonging to the fields of biology and molecular biology. The specific method is as follows: Using Phatr3_J33543 with the sequence structure shown in SEQ ID No.1, Phatr3_J50516 with the sequence structure shown in SEQ ID No.2, or Phatr3_Jdraft1806 with the sequence structure shown in SEQ ID No.3 as the target gene, removing the stop codon of the target gene, and connecting the fluorescent protein gene without the start codon through the DNA sequence encoding glycine to obtain a recombinant gene; inserting the recombinant gene into the multiple cloning site of the pPhaNR plasmid to obtain a vector plasmid; introducing the vector plasmid into diatom cells by electroporation and then inoculating them onto the ESAW solid selection medium containing bleomycin, and growing brown colonies; selecting the colonies with fluorescent protein signals in the colonies, which are the acid-resistant engineered algae. Compared with the wild algal species, the acid-resistant ability of the engineered algae obtained by the present invention is significantly improved. The present invention has important reference significance for the cultivation of acid-resistant diatoms.

The application discloses a kind of long-acting targeted cultivation alga high-density complex of water body and preparation method thereof, it is related to aquaculture field, the high-density complex includes the following weight parts components: small material premix 3-5 parts, sodium chloride 35-40 parts, gelatinized starch 1-3 parts, attapulgite 40-50 parts, cement 3-7 parts, titanate coupling agent 1-3 parts, molasses 2-4 parts and purified water 1-5 parts.The preparation method of the high-density complex includes the following steps: small material premix is subpackaged, is stirred uniformly with other components and adds molasses to carry out humidity control, then subpackaging and compression molding are formed, in turn plastic envelope, package and film winding.The long-acting targeted cultivation alga high-density complex of water body provided in the application can directional cultivation diatom and chlorella, and continuously release functional material, stabilize the abundance of beneficial algae, effectively solve the problem that directional cultivation beneficial organism cannot be carried out and functional material release speed is too fast.

The invention relates to the technical field of microbial culture, in particular to a heterotrophic culture method of benthic diatom, which comprises the following steps: S1, adding benthic diatom into an improved SE culture medium, and culturing for 5-7 days until OD540 is 0.8-1.2 to obtain a benthic diatom seed solution; according to the method disclosed by the invention, a nutrition system is constructed by taking waste glycerol as a carbon source and sodium alginate coated urea as a slow-release nitrogen source, and the benthic diatom, nitrogen-fixing bacteria and phosphate solubilizing bacteria are co-cultured on a porous ceramic carrier to be cultured under a dark condition, so that compared with the prior art, the method disclosed by the invention thoroughly gets rid of the dependence on illumination, and the yield is increased. The rapid, high-yield and stable heterotrophic culture of the benthic diatom is realized, meanwhile, the raw material and energy consumption cost is greatly reduced, and the resource recycling rate is improved; the obtained diatom biomass can be used for aquatic bait, biological energy, environmental restoration and development of high-added-value products, and has a wide industrial application prospect.

The application belongs to the technical field of cosmetics, and discloses a fucoidan polysaccharide composition with bacteriostatic efficacy, and a preparation method and application thereof. The fucoidan polysaccharide composition comprises phaeophycus triquetrum fucoidan, small new moon rhombus fucoidan and false micro-halochain fucoidan, and the mass ratio of the phaeophycus triquetrum fucoidan, the small new moon rhombus fucoidan and the false micro-halochain fucoidan is (1-2):(1-2):(1-2). The fucoidan polysaccharide composition is prepared by compounding specific phaeophycus triquetrum fucoidan, small new moon rhombus fucoidan and false micro-halochain fucoidan, has a synergistic effect, and has excellent antibacterial efficacy. When the fucoidan polysaccharide composition is applied to cosmetics, it has good formula adaptability, does not affect the exertion of efficacy and the stability of the cosmetics.

The invention provides a nutrition regulator based on bioactive substances and a preparation method of the nutrition regulator based on the bioactive substances. Comprising the following raw materials in parts by weight: 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of compound biological enzyme, 8-15 parts of algae auxin, 8-12 parts of compound probiotics, 3-8 parts of betaine, 15-25 parts of compound mineral elements and 5-10 parts of compound trace elements. According to the nutrient regulator, the algae auxin is formed by compounding the undaria pinnatifida fermentation product, the pomegranate peel fermentation product and the enteromorpha fermentation product, the undaria pinnatifida fermentation product promotes diatom growth, the enteromorpha fermentation product promotes green algae growth, the pomegranate peel fermentation product can inhibit growth of harmful algae such as blue-green algae, and the nutrient regulator has a synergistic effect on the algae auxin. Therefore, the proportion of beneficial algae such as diatom is increased and maintained for a long time, stable, efficient and high-quality water color beneficial to culture is formed, and the productivity structure of the pond is fundamentally optimized.

The invention discloses a diatom and dinoflagellate growth and population competition simulation method and system under pesticide stress. The method comprises the following steps: acquiring ecological environment parameters in a target sea area; the method comprises the following steps: constructing a pesticide biogeochemical process module, coupling the pesticide biogeochemical process module with a nutritive salt-diatom-dinoflagellate ecological dynamic model (NbPD), introducing photosynthesis enzyme activity regulation factors and pesticide effect factors of diatom and dinoflagellate into the coupled model, and establishing an agricultural input chemical geochemical-ecological dynamic model; based on the obtained ecological environment parameters, the model is used for simulating the growth process and population competition process of diatom and dinoflagellate after multiple groups of agricultural chemicals are added, and a dynamic response and dominant species replacement trend mechanism of the diatom and dinoflagellate is obtained. By means of the method, the ecological competition mechanism and succession path between the marine diatom and the dinoflagellate under the pesticide stress condition can be accurately reproduced, and a scientific tool and a simulation basis are provided for revealing the evolution rule of a phytoplankton community structure.

To provide an article made of a mineral composite material, and a method for manufacturing the same.SOLUTION: A composite material comprises by weight: one or more thermoplastic resins that are at least partially bio-based, the total percentage of the one or more thermoplastic resins being between 20% and 74.9%; a mineral filler having a seashell-based mineral material and a porous silica-based mineral material derived from diatom skeletons, a percentage of the mineral filler being between 25% and 79.9%; and a dispersant, a percentage of the dispersant being between 0.1% and 5%; optionally a stain system, a percentage of the stain system being between 0% and 5%; optionally a reinforcing material, a percentage of the reinforcing material being between 0% and 8%; and / or optionally a coupling agent, a percentage of the coupling agent being between 0% and 5%.SELECTED DRAWING: None

The present invention discloses a method for processing marine unicellular diatoms and a processed product thereof, comprising the following steps: step A: preparing fresh algae mud using fresh marine unicellular diatoms, and obtaining the pickled algae mud after pickling; step B: dissolving the polysaccharide in the pickled algae mud by microwave heating using urea-alkaline solution to obtain a first solution, ultrafiltering the first solution and concentrating, and obtaining chitosan composite polysaccharide powder through vacuum freeze drying; step C: granulating the chitosan composite polysaccharide powder using an ethanol-glycerol mixture, and obtaining a processed product after drying; the processed product is a chitosan composite polysaccharide particle, including chitosan and its derivatives, hemicellulose and cellulose. The processing method provided by the present invention is simple, and raw materials are easy to breed and enrich, and the processed chitosan composite polysaccharide has moisture-retaining properties and can be widely used in aquaculture feed, biomedicine, environmental engineering, engineering materials or chemical and chemical fields.

[Problem] It is an object of the present invention to provide a method for collecting seawater that contains plankton and producing DHMBA, which is an antioxidant, from the plankton contained in the seawater.[Solution] The method of the present invention includes: filtering collected seawater containing the plankton using a filter; taking out a cell content from the plankton remaining on the filter; subsequently heating / pressurizing the cell content taken out; and producing 3,5-dihydroxy-4-methoxybenzyl alcohol from the heated / pressurized product. The plankton is a diatom.

The invention provides a durable antibacterial environment-friendly marine antifouling coating for machinery as well as a preparation method and application of the durable antibacterial environment-friendly marine antifouling coating, and belongs to the technical field of marine antifouling materials. Nano titanium dioxide; a curing agent; a pigment; the fluorine-containing quaternary ammonium salt polysiloxane prepolymer is prepared by carrying out dehydration condensation reaction on a quaternary ammonium salt silane monomer A, a phenyl-containing silane monomer B and a fluorine-containing silane monomer C in an organic solvent under the action of a basic catalyst; the quaternary ammonium salt compound adopted by the invention has the characteristics of excellent environmental compatibility, outstanding fast-acting antibacterial and algae-killing properties and the like, can endow the surface of a material with continuous microbial inhibition and killing properties, and blocks the colonization and reproduction chain of marine attachment organisms from the source by destroying the integrity of bacterial cell membranes and interfering the activity of biological enzymes, so that the marine attachment organisms can be effectively inhibited. The resistance of the coating to typical fouling organisms such as barnacles and diatom is obviously improved; and meanwhile, the modulus and hardness of the coating can be remarkably improved, so that the coating can better resist scratch, abrasion and collision.

The application provides a method for preparing a coating layer by using diatom shells and application, comprising the following steps: 1, adding distilled water into diatom mud or diatom dry powder, adjusting pH value by using strong acid, and then standing to obtain precipitate, centrifuging and washing the obtained precipitate to obtain primary diatom shells; 2, digesting or calcining the primary diatom shells to remove organic matter to obtain inorganic diatom shells; 3, mixing the inorganic diatom shells or the primary diatom shells with anhydrous ethanol and ammonia water to obtain diatom shell sol; and 4, coating the diatom shell sol on the surface of a substrate and heat treating to obtain a coating layer. The application utilizes the porous structure, large specific surface area and high light trapping efficiency of diatom shells, and prepares diatom shell sol by using diatom mud or diatom dry powder as raw material to form a coating layer on a substrate, without the need of complex pretreatment and modification steps, so that the coating layer has high light transmittance, hydrophobic characteristics, good antireflection effect, and is widely applied to solar cells, automobile, greenhouse and building glass.

This application discloses a composite diatom powder live food water purifier for purifying water quality, a preparation method, and applications. The composite diatom powder live food water purifier comprises, by weight, 90-95 parts of a dormant diatom strain and 5-10 parts of a support matrix. The dormant diatom strains include dormant marine strains and dormant freshwater strains. The dormant marine strains include Isochrysis, Thalassiosira wissenii, Thalassiosira pseudodwarf, Nitzschia closterium, Cyclotella cryptica, Cyclotella meni, and Phaeodactylum triangularis. The dormant freshwater strains include Achillea millefolium, Navicula, and Cylindrospermum tenuis. The support matrix comprises filter soil. The composite diatom powder live food water purifier provided in this application has the ability to withstand harsh aquatic environmental conditions, remove ammonia, and produce high oxygen, and plays an important role in improving water quality.

The invention relates to the technical field of water body microorganism growth pollution and adhesion fouling treatment, in particular to a microorganism inactivation device and method for supplying power to a copper electrode through a friction nanometer generator, and the microorganism inactivation device comprises the friction nanometer generator, a copper oxide nanowire modified copper electrode, an electric field action area and a control system. A high-voltage and low-current electric field generated by the friction nano-generator is combined with the copper oxide nanowire modified copper electrode, dual synergy is achieved, a stable and controllable electric field source can be provided, the copper oxide nanowire modified copper electrode modifies the copper oxide nanowire with a high aspect ratio on the surface of a copper net, and when the copper electrode is powered, the copper oxide nanowire with the high aspect ratio can be provided for the copper electrode. Due to the lightning rod effect of the nanowires, the electric field of the tip part can generate an amplification phenomenon, and the remarkably enhanced electric field intensity can more effectively drive diatom and algal microorganisms in a water body and strongly destroy the integrity of cell membranes, so that cell contents are quickly leaked, and efficient physical inactivation is realized.

Methods for the fermentative production of O-phosphoserine, cysteate, or taurine in microbes or unicellular organisms that contain a serB mutation that either decreases serB expression, reduces the amount of the serB gene product or results in a serB gene product with low enzymatic activity. Genetic modifications of the O-phosphoserine, cysteate, or taurine and / or substrate biosynthetic pathways in unicellular organisms that include bacteria, algae, microalgae, diatoms, yeast, or fungi are disclosed. Also disclosed are fermentation and processing methods for the production of various O-phosphoserine-, cysteate-, or taurine-containing products and the use of the cells, fermentation broth or extracts that contain O-phosphoserine, cysteate, or taurine to produce products for use in food, feed, beverages, dietary and health supplements, cosmetics, personal care, pharmaceuticals, agricultural production, or surfactants.

The present invention relates to a method for the production of polyunsaturated fatty acids in an organism by introducing into the organism nucleic acids encoding polypeptides with Δ5-elongase activity. Advantageously, these nucleic acid sequences can be expressed in the organism if they are compatible with further nucleic acid sequences encoding polypeptides of fatty acid biosynthesis or lipid metabolism. Particularly advantageous are nucleic acid sequences encoding a Δ6-desaturase, Δ5-desaturase, Δ4-desaturase and / or Δ6-elongase activity. These desaturases and elongases are advantageously derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and / or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids.In a preferred embodiment, the present invention further relates to a method for producing unsaturated ω3 fatty acids and a method for producing triglycerides with an elevated content of unsaturated fatty acids, especially ω3 fatty acids with more than three double bonds.The invention relates to the creation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an elevated content of unsaturated ω3 fatty acids, oils or lipids with ω3 double bonds as a result of expression of elongases and desaturases used in the method according to the invention, advantageously together with ω3 desaturases, for example an ω3 desaturase from fungi of the family Pythiaceae such as the genus Phytophthora, for example the genus and species Phytophthora infestans or an ω3 desaturase from algae such as the family Prasinophyceae, for example the genus Ostreococcus, specifically the genus and species Ostreococcus tauri or diatoms such as the genus Thalassiosira, specifically the genus and species Thalassiosira pseudonana.The invention further relates to nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences of the invention, vectors comprising the nucleic acid sequences and / or nucleic acid constructs and transgenic organisms comprising the above-mentioned nucleic acid sequences, nucleic acid constructs and / or vectors. A further part of the invention relates to oils, lipids and / or fatty acids produced by the method of the invention and their use. The invention further relates to unsaturated fatty acids and triglycerides with an elevated content of unsaturated fatty acids and their use.

The invention relates to a ship body steel structure composite electrogenesis bacterium biological membrane anticorrosive coating and a preparation method and application thereof, and belongs to the technical field of marine ship steel structure anticorrosive coating preparation. According to the specific scheme, the ship body steel structure composite electrogenesis bacterium biological membrane anticorrosive coating comprises a coating I and a coating II, the outer surface of a ship body steel structure is coated with the coating I, and the outer surface of the ship body steel structure is coated with the coating II; the surface of the coating I is coated with the coating II, the coating I comprises diatom ooze I and seawater salt-tolerant anaerobic electricity-producing bacteria, and the seawater salt-tolerant anaerobic electricity-producing bacteria grow in pores of the diatom ooze I; the coating II comprises diatom ooze II and seawater salt-tolerant facultative anaerobic saccharomycetes, and the seawater salt-tolerant facultative anaerobic saccharomycetes grow in internal pores of the diatom ooze II. Corrosion prevention of the ship body steel structure is achieved through the environment-friendly microbial electrochemical technology, traditional epoxy resin and polyurethane anti-corrosion paint are replaced, and the technology belongs to the environment-friendly anti-corrosion technology.

The invention discloses a method for identifying biological siliceous shale, which comprises the following steps of: firstly, identifying a bright stripe layer in the biological siliceous shale as a diatom, calcium algae and radiation insect concentrated development section by utilizing an optical microscope, then quantitatively identifying the total siliceous mineral content of a sample by utilizing energy spectrum minerals, and measuring organic matters and porosity by utilizing a field emission scanning electron microscope. According to the method, the biological siliceous shale is identified from different microscales by adopting three methods, the results are mutually verified, and the method provides effective technical support for evaluating favorable layer sections of the shale and is of great significance for improving the exploration and development effect of shale gas.

The invention discloses an acid-resistant microalgae construction method based on bicarbonate ion transporter protein, and belongs to the field of biology and molecular biology. The method specifically comprises the following steps: taking Phatr3J33543 with a sequence structure as shown in SEQ ID No.1, Phatr3J50516 with a sequence structure as shown in SEQ ID No.2 or Phatr3Jdraft1806 with a sequence structure as shown in SEQ ID No.3 as a target gene, removing a termination codon of the target gene, and connecting a fluorescent protein gene with an initiation codon removed through a DNA (Deoxyribose Nucleic Acid) sequence for coding glycine to obtain a recombinant gene; inserting the recombinant gene into multiple cloning sites of a pPhaNR plasmid to obtain a vector plasmid; introducing the carrier plasmids into diatom cells through electroporation, and inoculating the diatom cells to an ESAW solid selective culture medium containing bleomycin to grow brown algal colonies; selecting the algal colonies with fluorescent protein signals from the algal colonies, namely the acid-resistant engineering algae. Compared with wild algae, the acid resistance of the engineering algae obtained by the invention is obviously improved. The method has important reference significance on acid-resistant diatom cultivation.

A method for detecting phytoplankton microorganisms based on deep learning includes the following steps: Step 1: preprocessing the original training data, organizing the data set into unlabeled samples and labeled samples, selecting diatoms, green algae and cyanobacteria as target categories in the labeled samples, and the format meets the COCO format requirements; Step 2: establishing a hybrid neural network structure for fast inference of image classification, combining attention bias and deformable convolution, using the backbone network to extract features, and combining a Deformable DETR detection model with better small target and density effects; Step 3: using the labeled data set as the input of the network; Step 4: training the network: first pre-training with a large number of unlabeled samples; using labeled data sets from diatoms, green algae and cyanobacteria to complete the downstream task of algae target detection. The present invention realizes fast and high-precision phytoplankton target detection.